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甘薯块根膨大机制研究进展

分子植物育种 2024年第20期22卷 6796-6804页

作者：宋炜涵后猛张允刚李强江苏徐淮地区徐州农业科学研究所徐州221131 农业农村部甘薯生物学与遗传育种重点实验室徐州221131

甘薯[Ipomoea batatas(L.)Lam.]兼粮食作物和经济作物,在中国粮食安全和经济发展中具有重要作用。甘薯块根是甘薯收获的主要器官,营养价值丰富,并且其发育及膨大直接影响甘薯的产量。因此,了解块根膨大的机理对提高甘薯产量有重要意义... 详细信息

甘薯[Ipomoea batatas(L.)Lam.]兼粮食作物和经济作物,在中国粮食安全和经济发展中具有重要作用。甘薯块根是甘薯收获的主要器官,营养价值丰富,并且其发育及膨大直接影响甘薯的产量。因此,了解块根膨大的机理对提高甘薯产量有重要意义。本研究从影响甘薯块根膨大的温度、光照、水分、施肥水平和塑料薄膜等环境因素,淀粉、可溶性糖、蛋白质和必需氨基酸等块根代谢物,细胞分裂素(CTK)、脱落酸(ABA)、生长素(IAA)、赤霉素(GA)和茉莉酸(JA)等内源激素,以及MADS-box和Homeobox等转录因子和淀粉合成、块根形成等相关基因4个方面综述了近年来有关甘薯块根膨大的研究进展,为甘薯块根膨大机理的深入研究提供参考。

关键词：甘薯块根膨大代谢物内源激素基因

Internal force and deformation matrixes and their applications in load path

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2010年第8期11卷 563-570页

作者： Qi CHEN Xin-jian kou Yan-meng ZHANG Department of Civil Engineering Shanghai Jiao Tong University Shanghai 200240 China

This paper deals with the internal force and the deformation matrixes, both of which can be used to analyze the topological relationship of a structure. Based on the reciprocal theorem, the relationship between the two matrixes is established, which greatly simplifies the computation of the internal force matrix. According to the characteristics of the internal force matrix, the transfer law of the matrix itself (due to the removal of components) is established based on the principle of linear superposition. With the relation of the two matrixes, the transfer law of the deformation matrix is also obtained. The transfer law illuminates the change regularity of internal force or deformation of the remnant structure when certain members are cut off one after another. The results of numerical examples show that the proposed methods are correct, reliable and effective.

关键词： Truss structure Redundancy Robustness Topological relationship of structure Removal of members Transfer law

生物炭-硅酸钙联合修复铅镉污染土壤的持续性效应

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农业资源与环境学报 2024年第2期41卷 473-481页

作者：苏梦贤张磊周小平寇萌熊娟汪明霞谭文峰华中农业大学资源与环境学院武汉430070

为探究棕榈生物炭与硅酸钙联合施用对Pb-Cd复合重金属污染土壤的钝化效果及其稳定性,本研究采用蔬菜种植盆栽实验,测定土壤溶液pH和Pb、Cd浓度以及蔬菜可食部分Pb、Cd含量。结果表明:与不添加钝化剂的对照组相比,添加钝化剂能够有效提... 详细信息

为探究棕榈生物炭与硅酸钙联合施用对Pb-Cd复合重金属污染土壤的钝化效果及其稳定性,本研究采用蔬菜种植盆栽实验,测定土壤溶液pH和Pb、Cd浓度以及蔬菜可食部分Pb、Cd含量。结果表明:与不添加钝化剂的对照组相比,添加钝化剂能够有效提高土壤溶液pH,使土壤溶液中Pb、Cd浓度显著降低,种植小白菜可食部分Pb含量下降62.20%~96.77%、Cd含量下降92.76%以上,多数处理符合国家食品安全标准。土壤种植适宜性研究结果表明,五种供试蔬菜可食部分重金属含量与富集系数规律一致,小白菜对Pb的富集能力最强,苋菜对Cd的富集能力最强。当土壤Pb0.6 mg·kg^(-1)、Pb>500 mg·kg^(-1)时,不适宜种植上述五种蔬菜。本研究探明了钝化剂的持续修复效果,研究结果可为土壤修复提及中低污染风险地区的适宜蔬菜种植提供技术和数据支撑。

关键词：土壤复合污染铅镉棕榈生物炭硅酸钙

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甘薯U6启动子克隆及其转录活性分析

江苏农业学报 2024年第6期40卷 969-974页

作者：唐维后猛宋炜涵闫会王欣李臣高闰飞张允刚李强江苏徐淮地区徐州农业科学研究所/农业农村部甘薯生物学与遗传育种重点实验室江苏徐州221131

在CRISPR/Cas9基因编辑系统中,U6启动子可以通过驱动sgRNA的表达来编辑目的基因,因而其转录活性会影响基因编辑效率。尽管人们已经在拟南芥、玉米、大豆、棉花等植物中开展了U6启动子的克隆与应用研究,然而目前在甘薯中U6启动子的相关... 详细信息

在CRISPR/Cas9基因编辑系统中,U6启动子可以通过驱动sgRNA的表达来编辑目的基因,因而其转录活性会影响基因编辑效率。尽管人们已经在拟南芥、玉米、大豆、棉花等植物中开展了U6启动子的克隆与应用研究,然而目前在甘薯中U6启动子的相关研究还未见报道。本研究利用拟南芥的AtU6 SnRNA的保守序列在三浅裂野牵牛(Ipomoea trifida)基因组数据库中搜索候选U6 RNA,然后在其上游搜索到2个不同的候选U6启动子,长度分别为526 bp(IbU6p-1)和532 bp(IbU6p-2);序列比对分析结果显示,甘薯的U6启动子具有上游序列元件(USE)以及TATA-box,其序列也与拟南芥高度相似。然后,利用获得的U6启动子核酸序列构建了能够驱动萤火虫荧光素酶基因(LUC)表达的重组框U6::LUC。最后,将含有上述重组载体的根癌农杆菌瞬时转化到本氏烟草叶片和甘薯愈伤组织中,并通过荧光成像技术分析荧光素酶活性。结果发现,在烟草及甘薯愈伤组织中2个甘薯U6启动子均能驱动LUC基因表达,具有转录活性。同时,IbU6p-2的转录活性无论是在烟草叶片中还是在甘薯愈伤组织中都显著高于拟南芥U6启动子。本研究结果为进一步发展甘薯基因编辑技术提供了参考。

关键词：甘薯 U6启动子克隆基因编辑转录活性

Sensitivity analyses of random cave groups on karst tunnel stability based on water–rock interaction using a novel contact dynamic method

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Underground Space 2024年第5期18卷 162-186页

作者： Chengzhi Xia Zhenming Shi Huanjia kou Shaoqiang meng Maomao Liu College of Civil Engineering Tongji UniversityShanghai 200092China Key Laboratory of Geotechnical and Underground Engineering of Ministry of Education Tongji UniversityShanghai 200092China

This paper concentrates on the sensitivity and dynamic simulation of randomly distributed karst cave groups on tunnel stability and connectivity extended ratio based on water–rock interaction using a novel contact dynamic method(CDM).The concept of karst cave group connectivity extended ratio during tunneling and water inrush is *** effects of cave shape and spatial distribution on Qiyueshan tunnel are *** deformation and damage index,and connectivity extended ratio with uniform random karst cave groups are *** results demonstrate that the connectivity extended ratio is verified as a crucial judgment in predicting the safe distance and assessing the stability of the tunnel with the karst cave *** model captures the fracture propagation and contact behavior of rock mass,surface flow,as well as the bidirectional water–rock interaction during the water inrush of Qiyueshan tunnel with multiple caves.A larger cave radius and smaller minimum distance between the cave and tunnel increase the deformation and damage index of the surrounding *** the cave radius and cave area ratio increase,the failure pattern shifts from overall to local *** findings potentially have broad applications in various surface and subsurface scenarios involving water–rock interactions.

关键词： Karst tunnel stability Contact dynamic method Connectivity extended ratio Karst cave groups Qiyueshan tunnel Water-rock interaction

Flexible assistance strategy of lower limb rehabilitation exoskeleton based on admittance model

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Science China(Technological Sciences) 2024年第3期67卷 823-834页

作者： kou JianGe WANG YiXuan CHEN ZhenLei SHI Yan GUO Qing XU meng School of Automation Science and Electrical Engineering Beihang UniversityBeijing 100191China School of Aeronautics and Astronautics University of Electronic Science and Technology of ChinaChengdu 611731China Department of Musculoskeletal Tumor Senior Department of Orthopedicsthe Fourth Medical Centerof PLA GeneralHospitalBeijing 100853China

Exercise training based on exoskeleton is an effective rehabilitation method for stroke patients. However, some rehabilitation exoskeletons still have poor wearable performance and obvious human-robot impedance during training, which easily cause secondary injuries to the patients. In this study, a variable admittance control strategy is proposed to improve the operator's wearable comfort, which can adapt to different operators by regulating admittance parameters. The admittance controller has two feedback loops: the position inner-loop and the admittance outer-loop. Meanwhile, the exoskeleton Lagrange model is constructed with unknown friction disturbance. By using Lyapunov technique and backstepping with state observer, the joint position error of exoskeleton is convergence into a zero neighborhood. The effectiveness of the proposed admittance controller is verified by both simulation and experiment. The tracking error of hip and knee joint is less than 4 degrees while the human-robot interaction torque is constrained in a tolerable range of the operator.

关键词： human-robot interaction exoskeleton backstepping admittance control

Morphological Evolution of Self-Assembled Sodium Dodecyl Sulfate/Dodecyltrimethylammonium Bromide@Epoxy-β-Cyclodextrin Supramolecular Aggregates Induced by Temperature

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Journal of Renewable Materials 2024年第4期12卷 629-641页

作者： Qingran meng Wenwen Xu Zuobing Xiao Qinfei Ke Xingran kou Key Laboratory of Textile Science&Technology Ministry of EducationCollege of TextilesDonghua UniversityShanghai201620China Collaborative Innovation Center of Fragrance Flavor and Cosmetics School of Perfume and Aroma Technology(Shanghai Research Institute of Fragrance&Flavour Industry)Shanghai Institute of TechnologyShanghaiChina

Bio-based cyclodextrins(CDs)are a common research object in supramolecular *** special cavity structure of CDs can form supramolecular self-assemblies such as vesicles and microcrystals through weak interaction with guest *** different forms of supramolecular self-assemblies can be transformed into each other under certain *** regulation of supramolecular self-assembly is not only helpful to understand the self-assembly principle,but also beneficial to its *** the present study,the self-assembly behavior of epoxy-β-cyclodextrin(EP-β-CD)and mixed anionic and cationic surfactant system(sodium dodecyl sulfate/dodecyltrimethylammonium bromide,SDS/DTAB)in aqueous solution was *** and particle size characterization found that the SDS/DTAB@EP-β-CD complex,as the basic building unit,self-assembled into worm-like micelles at lower temperatures and vesicles at higher *** magnetic resonance(NMR)and Fourier transform infrared spectroscopy(FT-IR)analysis revealed that the driving force for the formation of vesicles and worm-like micelles was the hydrogen bonds between EP-β-CD molecules,while water molecules played an important role in promoting vesicle formation between SDS/DTAB@EP-β-CD ***,the mechanism of the morphologic transformation of SDS/DTAB@EP-β-CD supramolecular aggregates induced by temperature was elucidated by exploring the self-assembly process,which may provide an excellent basis for the development of delivery carriers.

关键词： Epoxy-β-cyclodextrin SDS/DTAB self-assembly temperature morphological evolution

Inhibition of miR397 by STTM technology to increase sweetpotato resistance to SPVD

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Journal of Integrative Agriculture 2022年第10期21卷 2865-2875页

作者： LI Chen LIU Xuan-xuan ABOUELNASR Hesham MOHAMED HAMED Arisha kou meng TANG Wei YAN Hui WANG Xin WANG Xiao-xiao ZHANG Yun-gang LIU Ya-ju GAO Run-fei MA meng LI Qiang Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Sweetpotato Research Institute Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Breeding of SweetpotatoMinistry of Agriculture and Rural AffairsXuzhou 221131P.R.China School of Life Science Jiangsu Normal UniversityXuzhou 221116P.R.China Plant Pathology Department Agriculture and Biology Research DivisionNational Research CenterGiza 12622Egypt Department of Horticulture Faculty of AgricultureZagazig UniversityZagazig 44511Egypt

As a critical food crop,sweetpotato(Ipomoea batatas(L.)Lam.)is widely planted all over the world,but it is deeply affected by Sweetpotato Virus Disease(SPVD).The present study utilized short tandem target mimic(STTM)technology to effectively up-regulate the expression of laccase(Ib LACs)by successfully inhibiting the expression of mi *** upstream genes in the lignin synthesis pathway were widely up-regulated by feedback regulation,including phenylalanine ammonialyase(PAL),4-coumarate-Co Aligase(4 CL),hydroxycinnamoyl Co A:shikimatetransferase(HTC),caffeicacid O-methyltransferase(COMT),and cinnamyl alcohol dehydrogenase(CAD).Meanwhile,the activities of PAL and LAC increased significantly,finally leading to increased lignin *** deposition in the cell wall increased the physical defence ability of transgenic sweetpotato plants,reduced the accumulation of SPVD transmitted by Bemisia tabaci(Gennadius),and promoted healthy sweetpotato *** results provide new insights for disease resistance breeding and green production of sweetpotato.

关键词： sweetpotato miR397 STTM SPVD lignin content

Genome-wide analysis of expression quantitative trait loci(eQTLs)reveals the regulatory architecture of gene expression variation in the storage roots of sweet potato

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Horticulture Research 2020年第1期7卷 1713-1724页

作者： Lei Zhang Yicheng Yu Tianye Shi meng kou Jian Sun Tao Xu Qiang Li Shaoyuan Wu Qinghe Cao Wenqian Hou Zongyun Li Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics School of Life SciencesJiangsu Normal UniversityXuzhou 221116 Jiangsu ProvincePeople’s Republic of China Xuzhou Academy of Agricultural Sciences/Sweet Potato Research Institute CAASXuzhou 221121 Jiangsu ProvincePeople’s Republic of China

Dissecting the genetic regulation of gene expression is critical for understanding phenotypic variation and species ***,our understanding of the transcriptional variability in sweet potato remains ***,we analyzed two publicly available datasets to explore the landscape of transcriptomic variations and its genetic basis in the storage roots of sweet *** comprehensive analysis identified a total of 724,438 high-confidence single nucleotide polymorphisms(SNPs)and 26,026 expressed *** quantitative trait locus(eQTL)analysis revealed 4408 eQTLs regulating the expression of 3646 genes,including 2261 local eQTLs and 2147 distant *** distant eQTL hotspots were found with target genes significantly enriched in specific functional *** combining the information from regulatory network analyses,eQTLs and association mapping,we found that IbMYB1-2 acts as a master regulator and is the major gene responsible for the activation of anthocyanin biosynthesis in the storage roots of sweet *** study provides the first insight into the genetic architecture of genome-wide expression variation in sweet potato and can be used to investigate the potential effects of genetic variants on key agronomic traits in sweet potato.

关键词： roots analysis sweet