Inhibition of miR397 by STTM technology to increase sweetpotato resistance to SPVD

作     者：LI Chen LIU Xuan-xuan ABOUELNASR Hesham MOHAMED HAMED Arisha KOU Meng TANG Wei YAN Hui WANG Xin WANG Xiao-xiao ZHANG Yun-gang LIU Ya-ju GAO Run-fei MA Meng LI Qiang LI Chen;LIU Xuan-xuan;ABOUELNASR Hesham;MOHAMED HAMED Arisha;KOU Meng;TANG Wei;YAN Hui;WANG Xin;WANG Xiao-xiao;ZHANG Yun-gang;LIU Ya-ju;GAO Run-fei;MA Meng;LI Qiang

作者机构：Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Sweetpotato Research InstituteChinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Breeding of SweetpotatoMinistry of Agriculture and Rural AffairsXuzhou 221131P.R.China School of Life ScienceJiangsu Normal UniversityXuzhou 221116P.R.China Plant Pathology DepartmentAgriculture and Biology Research DivisionNational Research CenterGiza 12622Egypt Department of HorticultureFaculty of AgricultureZagazig UniversityZagazig 44511Egypt 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2022年第21卷第10期

页      面：2865-2875页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：financially supported by the National Key R&D Program of China (2019YFD1001300 and 2019YFD1001305) the earmarked fund for CARS-10-Sweetpotato the Jiangsu Postgraduate Scientific Research and Practical Innovation Program Project, China (KYCX19-2207) 

主　　题：sweetpotato miR397 STTM SPVD lignin content 

摘      要：As a critical food crop,sweetpotato(Ipomoea batatas(L.)Lam.)is widely planted all over the world,but it is deeply affected by Sweetpotato Virus Disease(SPVD).The present study utilized short tandem target mimic(STTM)technology to effectively up-regulate the expression of laccase(Ib LACs)by successfully inhibiting the expression of mi *** upstream genes in the lignin synthesis pathway were widely up-regulated by feedback regulation,including phenylalanine ammonialyase(PAL),4-coumarate-Co Aligase(4 CL),hydroxycinnamoyl Co A:shikimatetransferase(HTC),caffeicacid O-methyltransferase(COMT),and cinnamyl alcohol dehydrogenase(CAD).Meanwhile,the activities of PAL and LAC increased significantly,finally leading to increased lignin *** deposition in the cell wall increased the physical defence ability of transgenic sweetpotato plants,reduced the accumulation of SPVD transmitted by Bemisia tabaci(Gennadius),and promoted healthy sweetpotato *** results provide new insights for disease resistance breeding and green production of sweetpotato.