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Analysis of Genetic Diversity and Differentiation of Seven Stocks of Litopenaeus vannamei Using Microsatellite Markers

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Journal of Ocean University of China 2014年第4期13卷 647-656页

作者： ZHANG Kai WANG Weiji LI Weiya ZHANG Quanqi kong jie College of Marine Life Sciences Key Laboratory of Marine Genetics and Breeding (Ministry of Education) Ocean University of China Key Laboratory for Sustainable Utilization of Marine Fisheries Resources (Ministry of Agriculture) Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Shandong Marine Fishing and Production Management Station

Seven microsatellite markers were used to evaluate the genetic diversity and differentiation of seven stocks of Litopenaeus vannamei, which were introduced from Central and South America to China. All seven microsatellite loci were polymorphic, with polymorphism information content(PIC) values ranging from 0.593 to 0.952. Totally 92 alleles were identified, and the number of alleles(Na) and effective alleles(Ne) varied between 4 and 21 and 2.7 and 14.6, respectively. Observed heterozygosity(Ho) values were lower than the expected heterozygosity(He) values(0.526–0.754), which indicated that the seven stocks possessed a rich genetic diversity. Thirty-seven tests were detected for reasonable significant deviation from Hardy-Weinberg equilibrium. Fis values were positive at five loci, suggesting that there was a relatively high degree of inbreeding within stocks. Pairwise Fst values ranged from 0.0225 to 0.151, and most of the stock pairs were moderately differentiated. Genetic distance and cluster analysis using UPGMA revealed a close genetic relationship of L. vannamei between Pop2 and Pop3. AMOVA indicated that the genetic variation among stocks(11.3%) was much lower than that within stocks(88.7%). Although the seven stocks had a certain degree of genetic differentiation and a rich genetic diversity, there is an increasing risk of decreased performance due to inbreeding in subsequent generations.

关键词：遗传多样性分析微卫星标记凡纳滨对虾遗传分化 Hardy-Weinberg平衡多态信息含量等位基因近亲繁殖

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老年肝癌病人经肝动脉化疗栓塞术联合仑伐替尼治疗的疗效及安全性分析

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实用老年医学 2024年第4期38卷 362-366页

作者：孔杰姜锐刘正立何旭赵伯翔公茂峰王小平陆照璇顾建平张磊南京医科大学附属南京医院(南京市第一医院)普外科江苏省南京市210006

目的比较肝动脉化疗栓塞术(transcatheter arterial chemoembolization,TACE)单独治疗和联合仑伐替尼治疗老年中晚期肝细胞肝癌(HCC)的临床疗效及安全性。方法收集2018年6月至2020年8月南京市第一医院老年HCC病人的临床资料,根据治疗方... 详细信息

目的比较肝动脉化疗栓塞术(transcatheter arterial chemoembolization,TACE)单独治疗和联合仑伐替尼治疗老年中晚期肝细胞肝癌(HCC)的临床疗效及安全性。方法收集2018年6月至2020年8月南京市第一医院老年HCC病人的临床资料,根据治疗方法分为TACE组63例和TACE联合仑伐替尼组(联合组)76例,比较2组病人的肿瘤应答情况、生存情况及不良反应发生率。结果联合组的客观缓解率(43.7%)和疾病控制率(60.1%)均显著高于TACE组的23.8%、38.1%(P<0.001)。TACE组和联合组病人的中位无疾病进展时间(PFS)分别为7.5和12.5个月;中位生存时间(OS)分别为9.6和15.8个月,差异均有统计学意义(P<0.05)。2组随访过程中的不良反应均为Ⅰ~Ⅱ度,经过对症处理均有效控制。结论在老年中晚期HCC病人中,TACE联合仑伐替尼治疗方案可显著提高疗效,延长生存期,并且安全性良好。

关键词：经动脉化疗栓塞肝细胞肝癌仑伐替尼

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Is Human-induced Pluripotent Stem Cell the Best Optimal？

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Chinese Medical Journal 2018年第7期131卷 852-856页

作者： Feng Wang jie kong Yi-Yao Cui Peng Liu Jian-Yan Wen Peking University China-Japan Friendship School of Clinical Medicine Beijing 100029 China Department of Cardiovascular Surgery China-Japan Friendship Hospital Beijing 100029 China

Objective： Since the advent of induced pluripotent stem cell （iPSC） technology a decade ago, enormous progress has been made in stem cell biology and regenerative medicine. Human iPSCs have been widely used for disease modeling, drug discovery, and cell therapy development. In this review, we discuss the progress in applications of iPSC technology that are particularly relevant to drug discovery and regenerative medicine, and consider the remaining challenges and the emerging opportunities in the field. Data Sourees： Articles in this review were searched from PubMed database from January 2014 to December 2017. Study Selection： Original articles about iPSCs and cardiovascular diseases were included and analyzed. Results： iPSC holds great promises for human disease modeling, drug discovery, and stem cell-based therapy, and this potential is only beginning to be realized. However, several important issues remain to be addressed. Conelusions： The recent availability of human cardiomyocytes derived from iPSCs opens new opportunities to build in vitro models of cardiac disease, screening for new drugs and patient-specific cardiac therapy.

关键词： Cardiovascular Diseases Embryonic Stem Cells Induced Pluripotent Stem Cells

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Estimation of genetic parameters for growth trait of turbot using Bayesian and REML approaches

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Acta Oceanologica Sinica 2017年第6期36卷 47-51页

作者： GUAN Jiantao WANG Weiji HU Yulong WANG Mosang TIAN Tao kong jie Ocean University of China Qingdao 266003China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071China Shanghai Ocean University Shanghai 201306China

Bayesian and restricted maximum likelihood （REML） approaches were used to estimate the genetic parameters in a cultured turbot Scophthalmus maximus stock. The data set consisted of harvest body weight from 2 462 progenies （17 months old） from 28 families that were produced through artificial insemination using 39 parent fish. An animal model was applied to partition each weight value into a fixed effect, an additive genetic effect, and a residual effect. The average body weight of each family, which was measured at 110 days post-hatching, was considered as a covariate. For Bayesian analysis, heritability and breeding values were estimated using both the posterior mean and mode from the joint posterior conditional distribution. The results revealed that for additive genetic variance, the posterior mean estimate （σa^2 =9 320） was highest but with the smallest residual variance, REML estimates （σa^28 088） came second and the posterior mode estimate （σa^2=7 849） was lowest. The corresponding three heritability estimates followed the same trend as additive genetic variance and they were all high. The Pearson correlations between each pair of the three estimates of breeding values were all high, particularly that between the posterior mean and REML estimates （0.996 9）. These results reveal that the differences between Bayesian and REML methods in terms of estimation of heritability and breeding values were small. This study provides another feasible method of genetic parameter estimation in selective breeding programs of turbot.

关键词： turbot growth traits heritability breeding values REML Bayesian

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Estimates of heritability and correlation for growth traits of Turbot(Scophthalmus maximus L.) under low temperature conditions

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Acta Oceanologica Sinica 2015年第2期34卷 63-67页

作者： XU Liyong WANG Weiji kong jie LUAN Sheng HU Yulong MA Yu Wuxi Fisheries College Nanjing Agricultural University Key Laboratory of Genetic Resources and Breeding Laboratory Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences College of Fisheries and Life Science Shanghai Ocean University College of Fisheries and Life Science Dalian Ocean University

The objectives of this present research were to assess the heritability of growth traits under low temperature conditions in turbot（Scophthalmus maximus L.）, and to analyze the correlation between body weight（BW） and body length（BL）. There were 536 individuals from 25 full- and half-sib families involved in this study. During the entire 90-day period, which was initiated at 233 dph（day old） and ended at 323 dph, the individuals＇ BW and BL were weighed consecutively six times every 18 days. The heritability of BW and BL and the correlation between these two traits were estimated based on an individual animal model with the derivative-free restricted maximum likelihood（DFREML） method. These results showed that the specific growth rates（SGR） of 25 families was from 0.75±0.11 to 1.05±0.14 under water temperature of 10.5–12°C. In addition, the heritability of BW and BL estimated under low-temperature were 0.32±0.04 and 0.47±0.06, respectively. The BW had a medium heritability（0.2–0.4）, and the BL had a high heritability（〉0.45）, which suggested that selection for increased weight and length was feasible. Moreover, there was potential for mass selection on growth. The genetic and phenotypic correlations between BW and BL were 0.95±0.01 and 0.91±0.01（P 〈 0.01）, respectively. A significant correlation between BW and BL showed that BL could be instead of BW for indirect selection, which could be effectively implemented in the breeding program.

关键词： Scophthalmus maximus L. low-temperature heritability correlation

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Carbon-neutral pathway to mitigating transport-power grid cross-sector effects

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The Innovation 2024年第3期5卷 27-29页

作者： Jing Ma Huiwen kong Jianxiao Wang Haiwang Zhong Bo Li jie Song Daniel M.Kammen State Key Laboratory of Alternate Electrical Power System with Renewable Energy Sources School of Electrical and Electronic EngineeringNorth China Electric Power UniversityBeijing 102206China National Engineering Laboratory for Big Data Analysis and Applications Peking UniversityBeijing 100871China Peking University Ordos Research Institute of Energy Ordos 017000China State Key Laboratory of Power Systems and Generation Equipment Department of Electrical EngineeringTsinghua UniversityBeijing 100084China School of Electrical Engineering Guangxi UniversityNanning 530004China Department of Industrial Engineering and Management College of EngineeringPeking UniversityBeijing 100871China Energy and Resources Group and Goldman School of Public PolicyUniversity of CaliforniaBerkeleyBerkeleyCA 94720USA

Dear Editor,The growing demand for transportation energy has brought increasing challenges to reducing greenhouse gas ***,many countries or regions have proposed solutions to achieve carbon-neutral transportation such as the rapid expansion of the global electric vehicle(EV)***,these benefits are not *** the goal of decarbonization in electricity is achieved,the“pseudo net zero emissions”effect of the transportation sector will inevitably be accompanied by a quiet shifting of carbon responsibility.1 This shift is undoubtedly fatal for both net zero emissions at the national level and carbon responsibility at the industry level;however,the corresponding effects have not yet been clarified.

关键词： neutral doubt shifting

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Association between intestinal neoplasms and celiac disease:A review

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World Journal of Gastrointestinal Oncology 2021年第9期13卷 1017-1028页

作者： Man Wang Ming Yu Wen-jie kong Mei Cui Feng Gao Department of Gastroenterology People’s Hospital of Xinjiang Uygur Autonomous RegionUrumqi 830001Xinjiang Uygur Autonomous RegionChina Department of General Practice Xiangyang Central HospitalThe Affiliated Hospital of Hubei University of Arts and ScienceXiangyang 441021 Hubei ProvinceChina Department of Pathology People’s Hospital of Xinjiang Uygur Autonomous RegionUrumqi 830001Xinjiang Uygur Autonomous RegionChina

Celiac disease(CD)is a chronic immune-mediated intestinal disease with genetic *** is characterized by inflammatory damage to the small intestine after ingestion of cereals and products containing gluten *** recent years,the global prevalence rate of CD has been approximately 1%,and is gradually *** patients adhere to a gluten-free diet(GFD)throughout their entire ***,it is difficult to adhere strictly to a *** CD may be accompanied by gastrointestinal symptoms,such as diarrhea,abdominal pain,and extraintestinal symptoms caused by secondary *** studies have suggested that CD is associated with intestinal tumors such as enteropathyassociated T-cell lymphoma(EATL),small bowel cancer(SBC),and colorectal *** this study,we reviewed related studies published in the literature to provide a reference for the prevention and treatment of intestinal tumors in patients with *** with the general population,CD patients had a high total risk of SBC and EATL,but not colorectal *** protective effect of GFD on CD-related malignancies is *** studies are needed to confirm whether GFD treatment can reduce the risk of intestinal neoplasms in CD.

关键词： Celiac disease Gluten-free diet Intestinal neoplasms Small bowel cancer Enteropathy-associated T-cell lymphoma Colorectal cancer

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Research in migration route of hatchery released Chinese shrimp(Fenneropenaeus chinensis) in the Bohai Bay using method of SSR marker

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Acta Oceanologica Sinica 2020年第12期39卷 76-81页

作者： Weiji Wang Ding Lyu Mosang Wang Kefeng Liu jie kong Xiujuan Shan Xianshi Jin Key Laboratory of Sustainable Development of Marine Fisheries of Ministry of Agriculture Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Bohai Fisheries Research Institute of Tianjin Tianjin 300457China

This study provides new insights for the hatchery released Chinese shrimp(Fenneropenaeus chinensis),including proportion,dynamic migration route,after they were released into nature for stock enhancement using a new strategy quite different than *** shrimp were sampled at 22 survey stations during two investigation voyages acrossing 74 survey stations in the Bohai Sea from July 16 to August 9 in *** 289 sampled individuals during the second voyage,totally 155 shrimps were identified as hatchery shrimp released into the Laizhou Bay at mid-May in 2015 based on finger-print of eight SSR(simple sequence repeats)markers,and the proportion of hatchery released shrimp in recapture samples were from 41.30%–85.71%in each station with an average value 53.63%,which verified a previous view point that up to 90%of autumn season Chinese shrimp landing in the Bohai Sea were composed of hatchery ***,the dynamic migration route of hatchery released shrimp revealed that part of released shrimp migrated heading northwest along the west coast of the Bohai Sea up to the Bohai Bay but just remained at the Laizhou Bay until over-wintering migration at midOctober when they initiate over-wintering *** unnatural spring season shrimp fishing model cut the throat of spawner shrimp chance to swim back to their respective spawning plants at each spring,it still no chance to clarify whether the hatchery released shrimp could replenish to the reproduce population and complete a whole life cycle as same as their natural relatives.

关键词： Fenneropenaeus chinensis migration route hatchery release stock enhancement SSR marker

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Reducing the Common Environmental Effect on Litopenaeus vannamei Body Weight by Rearing Communally at Early Developmental Stages and Using a Reconstructed Pedigree

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Journal of Ocean University of China 2020年第4期19卷 923-930页

作者： kong Zhangwei kong jie HAO Dongchun LU Xia JIAN Tan MENG Xianhong LUO Kun CAO Baoxiang SUI Juan LI Xupeng LUAN Sheng Key Laboratory for Sustainable Utilization of Marine Fisheries Resources of Ministry of Agriculture Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266235China Fisheries College Ocean University of ChinaQingdao 266003China

Heritability estimates may be severely biased when a large common environmental effect on a family arises from a longlasting separate rearing at early stages(SRES)in traditional selective breeding programs,especially when bred populations have weak genetic *** rearing at early stages(CRES)may reduce common environmental effect since all families are reared in the same environment immediately after ***,we compared the effects of CRES and SRES strategies on genetic parameter estimation for harvest body weight in a selective breeding population of Litopenaeus vannamei with a small number of half-sib *** parameters of each strategy were estimated by using animal models excluding and including the common environmental effect(Model 1 and Model 2,respectively).Heritability estimates for body weight were 0.21±0.06(P0.05)in Model *** ratio of common environmental variance to phenotypic variance was 0.002±0.000 and 0.071±0.112 for CRES and SRES,*** strategy precisely partitioned the common environmental variance according to likelihood ratio *** heritability for body weight in CRES than in SRES implied that a large common environmental variance was confounded with additive genetic variance and was not effectively partitioned in ***,genetic correlation of body weight between the two strategies was 0.75±0.15,indicating that family rankings truly *** CRES should be followed in the selective breeding program of shrimp,especially in a population with a shallow pedigree and weak genetic ties between families.

关键词： common environmental effect heritability Litopenaeus vannamei parentage assignment

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引入高斯掩码自注意力模块的YOLOv3目标检测方法

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液晶与显示 2022年第4期37卷 539-548页

作者：孔雅洁张叶中国科学院长春光学精密机械与物理研究所应用光学国家重点实验室吉林长春130033 中国科学院大学北京100049

基于行车图像的目标检测方法为感知周围的道路环境提供了便宜、有效的解决方案,但同时也对检测效果和检测速度提出了较高要求。本文针对基于深度学习的一阶段目标检测算法YOLOv3展开研究,结合自注意力机制,在其网络深层结构中嵌入高斯... 详细信息

基于行车图像的目标检测方法为感知周围的道路环境提供了便宜、有效的解决方案,但同时也对检测效果和检测速度提出了较高要求。本文针对基于深度学习的一阶段目标检测算法YOLOv3展开研究,结合自注意力机制,在其网络深层结构中嵌入高斯掩码自注意力模块,缓解卷积操作感受野不足的缺陷,以捕捉更多的全局信息,提高算法的检测效果。实验结果表明,改进后的模型在MS COCO 2017数据集上训练结果的mAP@0.5达到56.88%,精度达到65.31%。与YOLOv3相比,mAP@0.5提高了2.56%,精度提高了3.53%。虽然检测速度有一些损失,但有效提高了模型的检测效果,能够更好地支撑辅助驾驶等应用。

关键词：目标检测 YOLOv3网络自注意力辅助驾驶

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