检索结果-南通市图书馆

Horticulture Research 2017年第1期4卷 301-310页

作者： Satish Kumar chris kirk Cecilia Deng Claudia Wiedow Mareike Knaebel Lester Brewer The New Zealand Institute for Plant&Food Research Limited Hawke’s Bay Research CentreHavelock NorthNew Zealand Palmerston North Research Centre Palmerston NorthNew Zealand Mount Albert Research Centre AucklandNew Zealand Motueka Research Centre MotuekaNew Zealand

Understanding of genetic diversity and marker-trait relationships in pears(Pyrus spp.)forms an important part of gene conservation and cultivar *** of Asian and European pear species,and interspecific hybrids were planted in a common garden ***-by-sequencing(GBS)was used to genotype 214 accessions,which were also phenotyped for fruit quality traits.A combination of selection scans and association analyses were used to identify signatures of *** of genetic diversity,population structure and introgression were also *** 15000 high-quality SNP markers were identified from the GBS data,of which 25%and 11%harboured private alleles for European and Asian species,*** clustering analysis suggested negligible gene flow,resulting in highly significant population differentiation(Fst=0.45)between Asian and European *** hybrids displayed an average of 55%and 45%introgression from their Asian and European ancestors,***(firmness,acidity,shape and so on)variation between accessions was significantly associated with genetic *** frequencies at large-effect SNP loci were significantly different between genetic groups,suggesting footprints of directional *** scan analyses identified over 20 outlier SNP loci with substantial statistical support,likely to be subject to directional selection or closely linked to loci under selection.

关键词： planted directional likely

Fine-mapping and validation of the genomic region underpinning pear red skin colour

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2019年第1期6卷 1607-1613页

作者： Satish Kumar chris kirk Cecilia Hong Deng Claudia Wiedow Mengfan Qin Richard Espley Jun Wu Lester Brewer The New Zealand Institute for Plant and Food Research Limited Hawkes Bay Research CentreHavelock NorthNew Zealand The New Zealand Institute for Plant and Food Research Limited Palmerston North Research CentrePalmerston NorthNew Zealand The New Zealand Institute for Plant and Food Research Limited Mount Albert Research CentreAucklandNew Zealand Centre of Pear Engineering Technology Research Nanjing Agricultural University210095 NanjingChina The New Zealand Institute for Plant and Food Research Limited Motueka Research CentreMotuekaNew Zealand

Red skin colour is an important target trait in various pear breeding *** this study,the genetic control of red skin colour was investigated in an interspecific population derived using the descendants of the red sport European pear cultivar‘Max Red Bartlett’(MRB)and the red-blushed Chinese pear cultivar‘Huobali’.Approximately 550 seedlings from nine families were phenotyped for red skin over-colour coverage(Ocolcov)and the intensity of red over-colour(Ocolint)on a 0–9 scale,and genotyped using ***-wide association analyses were conducted using 7500 high-quality single nucleotide polymorphisms(SNPs).Genomic regions on linkage groups(LG)4 and 5 were found to be associated,and the best SNP(S578_25116)on LG4 accounted for~15%of phenotypic variation in Ocolcov and *** association of S578_25116 with Ocolcov and Ocolint was successfully validated in a sample of~200 European and Asian pear *** association with red skin at locus S578_25116 was not present in Asian pear accessions,suggesting its close proximity to the MRB’s Cardinal *** putative candidate genes,including MYB transcription factors(PCP027962 and PCP027967),were identified in the quantitative trait locus region on LG4 and await functional validation.

关键词： cultivar breeding colour

Validation of SNP markers for fruit quality and disease resistance loci in apple (Malus × domestica Borkh.) using the OpenArray® platform

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2019年第1期6卷 1348-1363页

作者： David Chagné Stijn Vanderzande chris kirk Natalie Profitt Rosemary Weskett Susan E.Gardiner Cameron P.Peace Richard K.Volz Nahla V.Bassil The New Zealand Institute for Plant&Food Research Ltd(Plant&Food Research) Palmerston North Research CentrePalmerston NorthNew Zealand Department of Horticulture Washington State UniversityPullmanWAUSA Plant&Food Research Hawke’s Bay Research CentreHavelock NorthNew Zealand USDA-ARS National Clonal Germplasm RepositoryCorvallisORUSA

Genome mapping has promised much to tree fruit breeding during the last 10 ***,one of the greatest challenges remaining to tree fruit geneticists is the translation of trait loci and whole genome sequences into diagnostic genetic markers that are efficient and cost-effective for use by breeders,who must select genetically optimal parents and subsequently select genetically superior individuals among their *** take this translational step,we designed the apple International RosBREED SNP Consortium OpenArray v1.0(IRSCOA v1.0)assay using a set of 128 apple single nucleotide polymorphisms(SNPs)linked to fruit quality and pest and disease resistance trait *** Thermo Fisher Scientific OpenArray®technology enables multiplexed screening of SNP markers using a real-time PCR instrument with fluorescent probe-based Taqman®*** validated the apple IRSCOA v1.0 multitrait assay by screening 240 phenotyped individuals from the Plant&Food Research apple cultivar breeding *** set of individuals comprised commercial and heritage cultivars,elite selections,and families segregating for traits of importance to *** total,33 SNP markers of the IRSCOA v1.0 were validated for use in marker-assisted selection(MAS)for the scab resistances Rvi2/Vh2,Rvi4/Vh4,Rvi6/Vf,fire blight resistance MR5/RLP1,powdery mildew resistance Pl2,fruit firmness,skin colour,flavour intensity,and *** availability of this set of validated trait-associated SNP markers,which can be used individually on multiple genotyping platforms available to various apple breeding programmes or re-designed using the flanking sequences,represents a large translational genetics step from genomics to crop improvement of apple.

关键词： Open resistance breeding

Extreme-phenotype GWAS unravels a complex nexus between apple(Malus domestica)red-flesh colour and internal flesh browning

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fruit Research 2022年第1期2卷 110-123页

作者： Satish Kumar Cecilia H.Deng Claire Molloy chris kirk Blue Plunkett Kui Lin-Wang Andrew Allan Richard Espley The New Zealand Institute for Plant and Food Research Limited Hawke's Bay Research CentreHavelock North 4130New Zealand The New Zealand Institute for Plant and Food Research Limited Mount Albert Research CentreAuckland 1025New Zealand The New Zealand Institute for Plant and Food Research Limited Palmerston North Research CentrePalmerston North 4410New Zealand

The genetic link between apple red flesh(RF)coloration and the internal flesh browning disorder(FBD)is a major challenge when breeding high fruit quality RF apple cultivars.A genome-wide association study(GWAS)was conducted in a population of about 900 red-leaved seedlings to identify genomic regions and putative candidate genes using whole genome sequencing of the pools of extreme phenotypes(XP)for the RF colour coverage(using the weighted cortex index(WCI))and *** study identified novel genomic regions contributing to WCI and FBD variation in the red-leaved *** FBD-associated regions were enriched for genes regulating senescence,heat shock proteins,cytochrome P450,ascorbate metabolism and pectin methyl *** there were no significant regions in common for WCI and FBD,there were several genes(***85,MYB66,ethylene insensitive 3,DNAJ heat shock protein,WRKY7,and NAC42)enriched commonly between the genomic regions associated with these traits,potentially underpinning the genetic connection between WCI and *** of the differentially expressed genes between the R6:MdMYB10 and white-fleshed‘control’apples resided within the GWAS hotspot for WCI(*** synthase,UDP-Glycosyl transferase)and FBD(*** GTPase activating protein,lipoxygenase 1,phytoene synthase)–validating the XP-GWAS *** of several genes resided in the trait-associated genomic regions,suggesting that whole genome duplication plays an important role in the regulation of these *** genetic correlations between WCI and sensory traits were observed,and strategies to develop FBD-free high fruit quality RF cultivars are discussed.

关键词： cultivar breeding GWAS

中国实生和无性系苹果砧木及其杂交后代矮化基因型分子鉴定

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

果树学报 2020年第12期37卷 1779-1786页

作者：张恒涛 BUS Vincent G.M. 张亚茹王欢王美丽 BOWATTE Deepa GARDINER Susan E. CHAGNE David kirk chris KUMAR Satish 王大江张瑞萍周喆 YAO Jia-Long 阎振立中国农业科学院郑州果树研究所郑州450009 新西兰皇家植物与食品研究院 Havelock North 4157新西兰新西兰皇家植物与食品研究院 Palmerston North 4442新西兰中国农业科学院果树研究所辽宁兴城125100 新西兰皇家植物与食品研究院 Auckland 1142新西兰

【目的】利用已开发的矮化性状分子标记(Dw1/Dw2)筛选中国实生和自育无性系苹果砧木,探明各个砧木品种的矮化基因型,为苹果砧木育种工作中亲本选择及矮化分子辅助育种提供帮助。【方法】以不同地区采集的24份中国实生和自育的无性系苹... 详细信息

【目的】利用已开发的矮化性状分子标记(Dw1/Dw2)筛选中国实生和自育无性系苹果砧木,探明各个砧木品种的矮化基因型,为苹果砧木育种工作中亲本选择及矮化分子辅助育种提供帮助。【方法】以不同地区采集的24份中国实生和自育的无性系苹果砧木、147份新疆野苹果(李2-3)×‘M9’和345份山荆子(野1-2)×‘M9’杂交后代单株的叶片DNA为模板,以矮化砧木‘M9’‘M116’和‘MM106’为对照,利用已经开发的Dw1和Dw2矮化标记的SSR引物进行PCR扩增,然后用毛细管电泳检测PCR产物,分析确定各个样品的矮化基因型。【结果】在24份中国苹果砧木中鉴定出8份携带Dw1矮化基因,5份携带Dw2矮化基因,3个自育杂交品种同时携带Dw1和Dw2矮化基因;新疆野苹果(李2-3)×‘M9’组合的147个杂种实生苗中有48株携带Dw1矮化基因,14株同时携带Dw1和Dw2基因,山荆子(野1-2)×‘M9’的345个杂交后代中仅有11个单株携带Dw1基因型,其余单株均不携带任一矮化基因。【结论】试验中检测的八棱海棠(房2-3)、山荆子(野1-2)和平邑甜茶(野2-22)等中国常用实生砧木均不携带Dw1或Dw2基因,仅新疆野苹果(李2-3)中含有Dw2矮化基因;中国自育的杂交砧木品种中,以‘M9’等矮化品种为亲本的后代大多携带Dw1或Dw2;双亲都含有矮化基因的杂交组合后代能获得更多的携带矮化基因的单株。

关键词：苹果砧木矮化性状基因型分子标记育种

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

用于品牌推广和市场营销的最佳颜色

消费电子 2007年第14期 64-64页

作者： Jim Howard Heather kirk chris Howard Expert Business Source

我们对于颜色有着天生的敏感。我们看一种产品或者材料，最先注意到的东西之一就是颜色。我们对于这些颜色的看法会从心理上影响我们的感觉和看法。它们甚至会影响我们的购买欲望。

关键词：颜色市场营销品牌推广