Fine-mapping and validation of the genomic region underpinning pear red skin colour

作     者：Satish Kumar Chris Kirk Cecilia Hong Deng Claudia Wiedow Mengfan Qin Richard Espley Jun Wu Lester Brewer 

作者机构：The New Zealand Institute for Plant and Food Research LimitedHawkes Bay Research CentreHavelock NorthNew Zealand The New Zealand Institute for Plant and Food Research LimitedPalmerston North Research CentrePalmerston NorthNew Zealand The New Zealand Institute for Plant and Food Research LimitedMount Albert Research CentreAucklandNew Zealand Centre of Pear Engineering Technology ResearchNanjing Agricultural University210095 NanjingChina The New Zealand Institute for Plant and Food Research LimitedMotueka Research CentreMotuekaNew Zealand 

出 版 物：《Horticulture Research》 (园艺研究（英文）)

年 卷 期：2019年第6卷第1期

页      面：1607-1613页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：funded by the New Zealand Ministry of Business,Innovation and Employment(MBIE) Funding from the National ScienceFoundation of China(31820103012)is thankfully acknowledged 

主　　题：cultivar breeding colour 

摘      要：Red skin colour is an important target trait in various pear breeding *** this study,the genetic control of red skin colour was investigated in an interspecific population derived using the descendants of the red sport European pear cultivar‘Max Red Bartlett’(MRB)and the red-blushed Chinese pear cultivar‘Huobali’.Approximately 550 seedlings from nine families were phenotyped for red skin over-colour coverage(Ocolcov)and the intensity of red over-colour(Ocolint)on a 0–9 scale,and genotyped using ***-wide association analyses were conducted using 7500 high-quality single nucleotide polymorphisms(SNPs).Genomic regions on linkage groups(LG)4 and 5 were found to be associated,and the best SNP(S578_25116)on LG4 accounted for~15%of phenotypic variation in Ocolcov and *** association of S578_25116 with Ocolcov and Ocolint was successfully validated in a sample of~200 European and Asian pear *** association with red skin at locus S578_25116 was not present in Asian pear accessions,suggesting its close proximity to the MRB’s Cardinal *** putative candidate genes,including MYB transcription factors(PCP027962 and PCP027967),were identified in the quantitative trait locus region on LG4 and await functional validation.