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Resistance risk assessment of Puccinia striiformis *** to triadimefon in China

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Resistance risk assessment of Puccinia striiformis *** to tr...

中国植物病理学会2021年学术年会

作者： JI Fan LIU Yue ZHOU Aihong XIA Minghao ZHAO Jun ZHAN Gangming kang zhensheng College of plant protection Northwest A & F UniversityState Key Laboratory of Crop Stress Biology for Arid Areas

Wheat stripe rust,caused by Puccinia striiformis ***(Pst),is a destructive biological disaster on wheat and seriously threatens production safety in wheat producing areas which has been largely controlled using triadimefon in *** high levels of fungicide resistance in other pathogens have been reported,seldom field failure of Pst to any fungicides have been described and few fungicides sensitivity of Pst has been evaluated in *** resistance distribution and mechanism of Pst to triadimefon were investigated in the present study,providing important data for establishment of high throughput molecular detective methods,fungicide resistance risk management and the development of new target *** baseline sensitivity of 446 Pst isolates across the country to triadimefon was determined by detached leaf method,and the concentration for 50% of maximal effect(EC) distribution showed a unimodal curve,with mean value of 0.19 μg/*** results showed that the sensitivity range of Pst to triadimefon was wide,with more insensitive isolates collecting from winter-increasing area and northwest over-summering area and more sensitive isolates collecting from southwest over-summering area and two independent epidemic areas of Xinjiang and *** 6.79% of the tested Pst isolates had developed vary degrees of resistance to triadimefon,and most of isolates were sensitive to *** characterization of parasitic fitness,the triadimefon-resistant exhibited strong adaptive traits in urediniospore germination rate,latent period,sporulation intensity and lesion expansion *** cross-resistance was observed between triadimefon and tebuconazole or hexaconazole,but not between pyraclostrobin or *** point mutation Y134 F in 14 α-demethylase enzyme(Cyp51) was found in triadimefon-resistant *** a molecular detective method(Kompetitive Allele Specific PCR) was used for the rapid detection of the Y134 F point *** res

关键词： Puccinia striiformis f.sp.tritici wheat,stripe rust resistance risk

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中国小麦品种抗条锈病现状及存在问题与对策

植物保护 2018年第5期44卷 1-12页

作者：韩德俊康振生西北农林科技大学农学院旱区作物逆境国家重点实验室杨凌712100 西北农林科技大学植物保护学院旱区作物逆境国家重点实验室杨凌712100

小麦条锈病是小麦重大病害,利用抗病品种是防治小麦条锈病的有效措施。新中国成立以来,中国科学家们广泛开展了小麦条锈病防控方面的研究,并取得显著成绩。大量抗源被筛选、鉴定,但由于抗源自身农艺性状差等原因,能应用于小麦育种的抗... 详细信息

小麦条锈病是小麦重大病害,利用抗病品种是防治小麦条锈病的有效措施。新中国成立以来,中国科学家们广泛开展了小麦条锈病防控方面的研究,并取得显著成绩。大量抗源被筛选、鉴定,但由于抗源自身农艺性状差等原因,能应用于小麦育种的抗源亲本非常少,抗源利用不合理,导致生产品种抗病基因单一,病原菌流行小种很快形成,抗病品种在生产上可利用的寿命太短。本文介绍了国内外抗条锈病基因发掘情况,以及我国小麦抗条锈病育种抗病基因使用概况,通过回述1B/1R(Yr9)和Yr24/Yr26抗源的兴衰周期,总结了抗源利用的教训和经验,探讨了我国小麦抗条锈病育种中存在的问题和今后抗病基因合理利用的发展方向。

关键词：小麦抗条锈育种抗病基因兴衰周期抗病品种持久利用策略

Complete genomic sequenceand organization of a novel mycovirus from Phoma matteuciicola strain LG915

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Complete genomic sequenceand organization of a novel mycovir...

中国植物病理学会2019年学术年会

作者： Zheng Fan Xu Gang Zhou Jia Xie Changping Cui Hongguang Miao Weiguo kang zhensheng Zheng Li College of Plant Protection Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and PestsMinistry of Education State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F University

The complete genome of a double-stranded RNA(dsRNA) mycovirus,Phoma matteuciicola partitivirus 1(PmPV1) was *** consists of two dsRNA segments with 1 664 bp(dsRNA-1) and 1 383 bp(dsRNA-2) in length and each containing a single open reading frame(ORF)and potentially encoded 46.78 ku and 40.92 ku proteins,***-1 encodes a putative polypeptide with a conserved RNA—dependent RNA polymerase(RdRp) domain and showed similarity to that of *** protein encoded by dsRNA-2 has no significant similarity to the typical coat protein(CP) of the partitiviruses,while the structure analysis further suggested that might have the hypothetical function of coat *** viral particles of PmPV 1 were isometric and approximately 29 nm in *** analysis showed that PmPV1 is closely related to the Gammapartitivirus within the family Partitiviridae,but forming a separate branch with Colletotrichum acutatum RNA virus 1 and Ustilaginoidea virens partitivirus *** is the first report of the full-length nucleotide sequence of a novel virus classified in the genus Gammapartitivirus,infecting *** strain LG915,the causal agent of leaf blight of Curcuma wenyujin.

关键词： Phoma matteuciicola Mycovirus Gammapartitivirus Partitiviridae

小麦抗白粉病菌侵染乳突反应的超微结构与细胞化学

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浙江农业大学学报 1998年第5期24卷 502-508页

作者：胡东维李振岐浙江农业大学生物技术研究所

利用透射电镜对小麦白粉病菌与不同抗性寄主相互作用中乳突反应的超微结构进行一系统研究。结果表明，在超微结构上乳突反应是所有侵染位点的共有特征，线粒体，多聚核，高尔基体及各种小囊泡参与了小麦乳突的形成；进入乳突沉积区的... 详细信息

利用透射电镜对小麦白粉病菌与不同抗性寄主相互作用中乳突反应的超微结构进行一系统研究。结果表明，在超微结构上乳突反应是所有侵染位点的共有特征，线粒体，多聚核，高尔基体及各种小囊泡参与了小麦乳突的形成；进入乳突沉积区的大量小囊泡首先形成一个个结构致密的小颗粒，然后堆积起来形成乳突的内部主体和核心，其外围是细胞器解体后沉积的一层染色淡而均匀的无定形物质。

关键词：小麦超微结构细胞化学小麦白粉菌乳突反应

Novel Stripe Rust Effector Boosts the Transcription of a Host Negative Immune Factor Through Affecting Histone Modification to Promote Infection in Wheat

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Novel Stripe Rust Effector Boosts the Transcription of a Hos...

中国植物病理学会2023年学术年会

作者： DUAN Wanlu HAO Zhenkai PENG Yuxi PANG Huihui XU Yiwen ZHANG Ying kang zhensheng ZHAO Jing State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant Protection Northwest A&F University

The rust pathogen Puccinia striiformis ***(Pst) delivers diverse effector proteins into host cells to suppress the plant’s innate immunity,but whether Pst has evolved effector proteins that directly manipulate host gene transcription remains *** this study,we identified a Pst effector PstGTAl,which was specifically inducted during infection on wheat but not on *** contributes to the full virulence of Pst by activating the transcription level of wheat gene TraesCS3 B02 G319700,a negative regulator in wheat immunity but absent in barberry *** the infection,secreted PstGTAl is localized to wheat cell nucleus and targeted to the promoter of TraesCS3 B02 G319700,thereby activates the transcription of TraesCS3 B02 G319700 through increasing the H3 K4 acetylation levels,ultimately leading to the susceptibility of ***,our study reveals a virulence strategy of a rust effector in modulating host gene expression by directly binding to the promoter and affecting histone modification,providing insights into the pathogenic mechanism that evolved in a plant pathogen to facilitate infection at the epigenetic level.

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小麦内生细菌及其对根茎部主要病原真菌的抑制作用

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应用生态学报 2006年第4期17卷 690-694页

作者：乔宏萍黄丽丽康振生西北农林科技大学植物保护学院杨凌712100

对小麦植株不同生育期、不同器官的内生细菌进行了分离和数量变化分析.结果表明,根、茎、叶及未成熟籽粒等器官中存在大量的内生细菌,鲜组织中平均约含内生细菌5.0×105CFU.g-1,其中根系中内生细菌数量达7.8×105CFU.g-1,而茎秆、叶片... 详细信息

对小麦植株不同生育期、不同器官的内生细菌进行了分离和数量变化分析.结果表明,根、茎、叶及未成熟籽粒等器官中存在大量的内生细菌,鲜组织中平均约含内生细菌5.0×105CFU.g-1,其中根系中内生细菌数量达7.8×105CFU.g-1,而茎秆、叶片和未成熟籽粒中内生细菌数量分别为4.8×105、3.2×105和2.8×105CFU.g-1.内生细菌数量在不同生育期也存在差异,幼苗期平均约为3.1×105CFU.g-1、拔节期和灌浆期分别为5.7×105和7.0×105CFU.g-1.不同小麦田块之间存在明显差异,长武县一田块植物鲜组织中内生细菌的数量为6.1×105CFU.g-1,而大荔县一田块约为3.9×105CFU.g-1.试验结果发现,对小麦全蚀病菌具有拮抗作用的内生细菌有51株、对小麦纹枯病菌具有抑制作用的内生细菌有45株.用平板对峙法测定,有71株对两种病原真菌均有拮抗作用,对小麦全蚀病菌抑菌圈直径大于10mm的有23株,其中来源于根系、茎秆、叶片和籽粒的分别为6株、7株、9株和1株;对小麦纹枯病菌抑菌圈超过10 mm的有20株,其中来源于根系、茎秆、叶片和籽粒的分别为7株、5株、7株和1株,说明从小麦叶片诱捕分离的内生细菌中,对小麦全蚀病菌和纹枯病菌抑菌作用较强的分离株比率最高,其次为茎秆,而根部和未成熟籽粒中比例明显较低.

关键词：抑菌圈菌落形成单位诱捕分离小麦全蚀病菌小麦纹枯病菌

Cytology of Interaction Between Wheat and Puccinia striiformin

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Cytology of Interaction Between Wheat and Puccinia striiform...

The 7th Korea-China Joint Symposium for Mycology

作者： kang zhensheng College of Plant Protection Northwest A&F University Yangling Shaanxi 712100 P.R.China

＜正＞Wheat stripe rust, caused by Puccinia striiformis Westend f. sp. tritici, occurs worldwide and is considered a major disease in temperate regions, particularly in China. It has been demonstrated that the reasona... 详细信息

关键词： Host resistant mechanism Immunogold labeling techniques Puccinia striiformis Wheat stripe rust

Trace Lasso Regularization for Adaptive Sparse Canonical Correlation Analysis via Manifold Optimization Approach

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Journal of the Operations Research Society of China 2024年第3期12卷 573-599页

作者： kang-kang Deng Zheng Peng Beijing International Center for Mathematical Research Peking UniversityBeijing100871China School of Mathematics and Computational Science Xiangtan UniversityXiangtan411105China

Canonical correlation analysis(CCA)describes the relationship between two sets of variables by finding a linear combination that maximizes the correlation ***,in high-dimensional settings where the number of variables exceeds sample size,or in the case that the variables are highly correlated,the traditional CCA is no longer *** this paper,a new matrix regularization is introduced,which is an extension of the trace Lasso in the vector *** we propose an adaptive sparse version of CCA(ASCCA)to overcome these disadvantages by utilizing the trace Lasso *** adaptability of ASCCA is that the sparsity regularization of canonical vectors depends on the sample data,which is more realistic in practical *** ASCCA model is further reformulated to an optimization problem on the Riemannian *** we adopt a manifold inexact augmented Lagrangian method to solve the resulting optimization *** performance of the ASCCA model is compared with some existing sparse CCA techniques in different simulation settings and real datasets.

关键词： Canonical correlation analysis Sparsity of canonical vectors Trace Lasso regularization Manifold optimization

Roles of Brachypodium distachyon WRKY67 transcription factor in plant responses to Brachypodium rustF-co

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Roles of Brachypodium distachyon WRKY67 transcription factor...

中国植物病理学会2021年学术年会

作者： HE Mengying WANG Ning FAN Xin ZHANG Shan HU Zeyu WANG Jianfeng TANG Chunlei kang zhensheng WANG Xiaojie State Key Laboratory Crop Stress Biology for Arid Area/College of Plant Protection Northwest A＆F University

WRKY protein is a pivotal type of transcription factor involved in plant growth and development,bioticand abioticstress,and plant defense *** distachyonwith its short life cycle,small genome and close evolutionary relationship of wheat is an ideal model plant for studying the interaction of plant pathogens and *** on Brachypodium genomicanalysis and gene expression microarray,the BdWRKY67 gene,which is closely related to wheat evolution and induced by rust fungus,was selected for an in-depth *** qRT-PCR,we onfirmed that BdWRKY67 gene was highly induced at 48 h post Brachypodium rust F-co *** phenotypic identification and histological observation of BdWRKY67 RNAi plants,BdWRKY67 RNAi plants confered resistant to Brachypodium rust compared with wild-type *** results preliminarily indicate that the BdWRKY67 transcription factor participate in negative regulation during the interaction between Brachypodium brachypodium and Brachypodium *** study preliminarily clarified the function of BdWRKY67,and provided a theoretical basis for further revealing the interaction mechanism between wheat WRKY transcription factor homologous to Brachypodium and stripe rust *** the Brachypodium distachyon-brachypodium rust interaction system can accelerate the research on the molecular mechanism of rust disease,and provide onvenience for the in-depth and rapid revealing of the interaction mechanism between wheat and rust fungus.

关键词： Brachypodium distachyon WRKY transcription factor Brachypodium rust RNAi