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Roles of Brachypodium distachyon WRKY67 transcription factor...

Roles of Brachypodium distachyon WRKY67 transcription factor in plant responses to Brachypodium rustF-co

作     者:HE Mengying WANG Ning FAN Xin ZHANG Shan HU Zeyu WANG Jianfeng TANG Chunlei KANG Zhensheng WANG Xiaojie 

作者单位:State Key Laboratory Crop Stress Biology for Arid Area/College of Plant ProtectionNorthwest A&F University 

会议名称:《中国植物病理学会2021年学术年会》

会议日期:2021年

学科分类:09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

关 键 词:Brachypodium distachyon WRKY transcription factor Brachypodium rust RNAi 

摘      要:WRKY protein is a pivotal type of transcription factor involved in plant growth and development,bioticand abioticstress,and plant defense *** distachyonwith its short life cycle,small genome and close evolutionary relationship of wheat is an ideal model plant for studying the interaction of plant pathogens and *** on Brachypodium genomicanalysis and gene expression microarray,the BdWRKY67 gene,which is closely related to wheat evolution and induced by rust fungus,was selected for an in-depth *** qRT-PCR,we onfirmed that BdWRKY67 gene was highly induced at 48 h post Brachypodium rust F-co *** phenotypic identification and histological observation of BdWRKY67 RNAi plants,BdWRKY67 RNAi plants confered resistant to Brachypodium rust compared with wild-type *** results preliminarily indicate that the BdWRKY67 transcription factor participate in negative regulation during the interaction between Brachypodium brachypodium and Brachypodium *** study preliminarily clarified the function of BdWRKY67,and provided a theoretical basis for further revealing the interaction mechanism between wheat WRKY transcription factor homologous to Brachypodium and stripe rust *** the Brachypodium distachyon-brachypodium rust interaction system can accelerate the research on the molecular mechanism of rust disease,and provide onvenience for the in-depth and rapid revealing of the interaction mechanism between wheat and rust fungus.

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