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Case Control Study of Post-endoscopic Variceal Ligation Bleeding Ulcers in Severe Liver Disease: Outcomes and Management

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Journal of Clinical and Translational Hepatology 2019年第1期7卷 32-39页

作者： kapil D.Jamwal Rakhi Maiwall manoj k.Sharma Guresh kumar Shiv k.Sarin Department of Gastroenterology Artemis Hospital GurugramHaryanaIndia Department of Hepatology Institute of Liver and Biliary SciencesNew DelhiIndia Department of Gastroenterology Dharamshila Narayana Superspeciality HospitalNew DelhiIndia Department of Research and Biostatistics Institute of Liver and Biliary SciencesNew DelhiIndia

Background and Aims:The management of post-endoscopic variceal ligation(EVL)bleeding ulcers(PEBUs)is currently based on local expertise and patients liver disease *** present retrospective study investigated associations between the endoscopic morphology of PEBUs and patient ***:Patients underwent EVL(primary or secondary),from January 2015 to January 2018,in two tertiary care hospitals in India(ILBS New Delhi and Dharamshila Narayana New Delhi).Mortality rates were determined at post-EVL day five and week *** were typified based on Jamwal&Sarin classification system as follows:A,ulcer with active spurting;B,ulcer with ooze;C,ulcer base with visible vessel or clot;and D,clean or pigmented ***:Of 3854 EVL procedures,141(3.6%)patients developed PEBU,and 46/141(32.6%)suffered *** the former,the PEBU types A,B,C,and D accounted for 17.7,26.2,36.3,and 19.8%,*** those who died,39.1,30.4,21.7,and 8.8%had PEBU types A,B,C,and *** included transjugular intrahepatic portosystemic shunts(TIPS),esophageal self-expandable metal stent(SEMS),glue and sclerosant injection,Sengstaken-Blakemore tube placement and liver *** univariate analysis,no correlation with hepatic venous pressure gradient,TIPS placement,size of varices,or number of bands was *** Model for EndStage Liver Disease(MELD)-sodium score correlated positively with *** adjusting for MELD-sodium score,mortality was best predicted by type-A ulcer(p=0.024;OR 8.95,CI 1.34-59.72).Conclusions:PEBU occurred in 3.6%of a large EVL *** patients based on PEBU type can help predict outcomes,independent of the MELD-sodium *** PEBUs by endoscopic morphology may inform treatment strategies,and warrants further validation.

关键词： Post-endoscopic variceal ligation bleeding ulcers Severe liver disease Case control study Clinical outcomes

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Development of a Polyclonal Antibody-based AC-ELISA and Its Comparison with PCR for Diagnosis of Canine Parvovirus Infection

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Virologica Sinica 2010年第5期25卷 352-360页

作者： manoj kumar Sukdeb Nandi Sunil Chidri Virology Laboratory Centre for Animal Disease Research and DiagnosisIndian Veterinary Research Institute

A polyclonal antibody-based antigen-capture ELISA （AC-ELISA） has been developed for detection of Canine parvovirus （CPV） antigens in faecal samples of dogs. The assay uses rabbit anti-CPV polyclonal antibody as the capture antibody, guinea pig anti-CPV polyclonal antibody as tracing antibody and anti-guinea pig HRPO conjugate as the detection system. The optimum dilution of the capture antibody and the tracing antibody capable of detecting the CPV-2 antigens was found to be 1：1 600 and 1：400, respectively, in the check-board titration. In this study, a total of 152 samples （129 faecal samples and 23 cell culture supernatant） were tested both by AC-ELISA and by polymerase chain reaction （PCR）. Of the samples tested, 69 and 78 samples were found positive by AC-ELISA and PCR, respectively. The AC-ELISA had relative sensitivity, relative specificity and accuracy of 88.4%, 100.0% and 91.4% respectively. The analytical sensitivity of AC-ELISA was estimated to be 102.8 TCID50/mL whereas PCR sensitivity was 100.8 TCIDs0/mL. The AC-ELISA is a simple, quick and reliable method for screening large numbers of faecal samples of dogs suspected of CPV infection.

关键词： Canine parvovirus (CPV) Polyclonal antibody Antigen-capture ELISA(AC-ELISA)

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Analysis and use of fuzzy intelligent technique for navigation of humanoid robot in obstacle prone zone

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Defence Technology（防务技术） 2018年第6期14卷 677-682页

作者： Asita kumar Rath Dayal R.Parhi Harish Chandra Das manoj kumar Muni Priyadarshi Biplab kumar Centre of Biomechanical Science Siksha O'Anusandhan Deemed to be University Robotics Laboratory Mechanical Engineering DepartmentNational Institute of Technology(India) Mechanical Engineering Department National Institute of Technology(India)

With the increasing use of humanoid robots in several sectors of industrial automation and manufacturing, navigation and path planning of humanoids has emerged as one of the most promising area of research. In this paper, a navigational controller has been developed for a humanoid by using fuzzy logic as an intelligent algorithm for avoiding the obstacles present in the environment and reach the desired target position safely. Here, the controller has been designed by careful consideration of the navigational parameters by the help of fuzzy rules. The sensory information regarding obstacle distances and bearing angle towards the target are considered as inputs to the controller and necessary velocities for avoiding the obstacles are obtained as outputs. The working of the controller has been tested on a NAO humanoid robot in V-REP simulation platform. To validate the simulation results, an experimental platform has been designed under laboratory conditions, and experimental analysis has been ***, the results obtained from both the environments are compared against each other with a good agreement between them.

关键词： Humanoid robot Navigation Path planning Fuzzy

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Application of quasi-oppositional symbiotic organisms search based extreme learning machine for stock market prediction

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International Journal of Intelligent Computing and Cybernetics 2019年第2期12卷 175-193页

作者： Smita Rath Binod kumar Sahu manoj Ranjan Nayak Institute of Technical Education and Research Siksha O Anusandhan UniversityBhubaneswarIndia

Purpose–Forecasting of stock indices is a challenging issue because stock data are dynamic,non-linear and uncertain in *** of an accurate forecasting model is very much essential to predict the next-day closing prices of the stock *** purpose of this paper is to develop an efficient and accurate forecasting model to predict the next-day closing prices of seven stock ***/methodology/approach–A novel strategy called quasi-oppositional symbiotic organisms search-based extreme learning machine(QSOS-ELM)is proposed to forecast the next-day closing prices *** in the prediction of closing price depends on output weights which are dependent on input weights and *** paper mainly deals with the optimal design of input weights and biases of the ELM prediction model using QSOS and SOS optimization ***–Simulation is carried out on seven stock indices,and performance analysis of QSOS-ELM and SOS-ELM prediction models is done by taking various statistical measures such as mean square error,mean absolute percentage error,accuracy and paired sample *** performance analysis reveals that the QSOS-ELM model outperforms the SOS-ELM model in predicting the next-day closing prices more accurately for all the seven stock indices under ***/value–The QSOS-ELM prediction model and SOS-ELM are developed for the first time to predict the next-day closing prices of various stock *** paired t-test is also carried out for the first time in literature to hypothetically prove that there is a zero mean difference between the predicted and actual closing prices.

关键词： Extreme learning machine Symbiotic organisms search Quasi-oppositional-based learning,Paired sample t-test

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Removal efficiency of fluoride by novel Mg-Cr-Cl layered double hydroxide by batch process from water

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Journal of Environmental Sciences 2013年第5期25卷 993-1000页

作者： Sandip Mandal Swagatika Tripathy Tapswani Padhi manoj kumar Sahu Raj kishore Patel Department of Chemistry National Institute of TechnologyRourkela 769 008India

The fluoride ion removal from aqueous solution using synthesized Mg-Cr-Cl layered double hydroxide has been ***-Cr-Cl was characterized by X-ray powder diffraction,Fourier-transform infrared,thermo-gravimetric analysis,differential thermal analysis,and scanning electron *** experiments were carried out in batch mode as a function of adsorption dosages,contact time,pH,and initial fluoride concentration to get optimum adsorption *** adsorption kinetic study showed that the adsorption process followed first order *** fluoride removal was 88.5% and 77.4% at pH 7 with an adsorbent dose of 0.6 g/100 mL solution and initial fluoride concentration of 10 mg/L and 100 mg/L,*** equilibrium was established at 40 *** experiment data were fitted well with Langmuir isotherm with R 2 = *** constants were also measured and concluded that the adsorption process was spontaneous and endothermic in *** removal percentage decreased slowly with increasing *** process is suitable for industrial *** regeneration of the material is not possible.

关键词： layered double hydroxide(LDH) fluoride adsorbents

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Identification and characterization of genotype A and D recombinant hepatitis B virus from Indian chronic HBV isolates

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World Journal of Gastroenterology 2008年第40期14卷 6228-6236页

作者： Ranjit Chauhan Syed Naqui kazim manoj kumar Jayashree Bhattacharjee Narayanasamy krishnamoorthy Shiv kumar Sarin Department of Gastroenterology and Advanced Center for Liver Diseases G.B.Pant Hospital Department of Biochemistry Lady Hardinge Medical College Centre for Interdisciplinary Research in Basic Sciences The Centre for Genomic Application

AIM： To confirm the presence of recombination, fulllength hepatitis B virus （HBV） from chronic patients was sequenced and analyzed. METHODS： Full-length HBV genomes from 12 patients were amplified and sequenced in an automated sequencer. Phylogenetic analysis was carried out on full-length, Core and preS2/Surface regions using MEGA software. SimPIot Boot Scanning and amino acid sequence analysis were performed for confirmation of recombination. RESULTS： Eight patients were infected with genotype D strain; one patient with genotype A and three patients had genotype A and D recombination; two of them had cirrhosis and one had hepatocellular carcinoma. Phylogenetic analysis of core and preS2/surface regions separately showed evidence of genotype A and D recombination. The breakpoints of recombination were found to be at the start of preS2 and at the endof surface coding regions. CONCLUSION： We identified and characterized recombinant A and D genotype HBV in hepatitis B surface antigen （HBsAg）-positive patients.

关键词： Hepatitis B virus Genotype Variation Evolution Recombination

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Feasibility of Al−TiC coating on AZ91 magnesium alloy by TIG alloying method for tribological application

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Transactions of Nonferrous Metals Society of China 2020年第6期30卷 1550-1559页

作者： Chinmaya kumar PADHEE manoj MASANTA A.k.MONDAL Department of Mechanical Engineering National Institute of Technology RourkelaRourkela 769008India Department of Metallurgical Engineering Indian Institute of Technology(BHU)VaranasiVaranasi 221005India

An attempt was made to improve the surface properties of the AZ91 Mg alloy through surface alloying of a mixture of Al and TiC with the help of TIG arc as heat *** microstructural evolution of the alloyed layer on the AZ91 alloy was analysed through SEM and XRD *** micro-hardness and the dry sliding wear behaviour were assessed by Vickers micro-hardness tester and pin-on-disc wear test setup,*** is revealed that almost uniform alloyed layer forms on the AZ91 alloy substrate for a specific current and scan speed employed in the present *** alloyed layer exhibits hardness value up to 305 HV0.25,and almost negligible wear as compared to the as-received AZ91 alloy substrate.

关键词： AZ91 magnesium alloy Al-TiC coating TIG alloying micro-hardness dry sliding wear

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Design of an Efficient Cooperative Spectrum for Intra-Hospital Cognitive Radio Network

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Computers, Materials & Continua 2021年第10期69卷 35-49页

作者： Abhinav Adarsh Basant kumar manoj Gupta Arun kumar Aman Singh Mehedi Masud Fahad A.Alraddady Department of ECE MNNIT AllahabadPrayagraj211004India Department of ECE JECRC UniversityJaipur303905India Computer Science and Engineering Lovely Professional UniversityPunjab144411India Department of Computer Science College of Computers and Information TechnologyTaif UniversityP.O.Box 11099Taif21944Saudi Arabia Department of Computer Engineering College of Computers and Information TechnologyTaif UniversityP.O.Box 11099Taif21944Saudi Arabia

This paper presents a new low delay centralized cooperative spectrum sensing method based on dynamic voting rule using multiple threshold level,for indoor hospital environment,where fading and direct path loss both are together responsible for variation in signal *** the proposed algorithm,weights of Cognitive Radios(CRs)in terms of assigned votecount are estimated based on the sensed energy values with respect to multiple fixed threshold levels,so that the direct path loss can be dealt without further increasing the error,which occurs due to *** indoor hospital like environment,Rician fading model is more ***,formulation,evaluation and analysis of various sensing performance parameters have been carried out considering Rician fading *** analysis and tradeoff among three optimum parameters-energy threshold,total number of CRs participating in sensing and total error rate,are presented with respect to signal to noise ratio(SNR).Finally,the Monte Carlo simulation is designed and implemented considering the two conditions-only fading environment and fading along with direct path loss *** can be observed that in both cases,the proposed spectrum sensing rule outperforms the other contemporary rules.

关键词： Cognitive radio direct path loss spectrum sensing health care

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Mineralogy and textural impact on beneficiation of goethitic ore

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International Journal of Mining Science and Technology 2017年第3期27卷 445-450页

作者： Dey Shobhana Mohanta manoj k. Singh Ratnakar Mineral Processing Division CSIR-National Metallurgical Laboratory Jamshedpur 831007 India

The effect of mineralogy and texture on the beneficiation of goethitic ores from two different origins is highlighted. Sample A having 54.47% Fe with 8.57% loss of ignition （LOl） indicates the presence of vitreous and ochreous goethite, martite and microplaty hematite as the major minerals. Sample B contains 56.90% Fe with 14.4% LOI. There is a pisolithic laterite containing vitreous and ochreous goethite, quartz, kaolinitic clay and there is no hematite mineral. The liberated minerals in -150 ＋ 100 μm size class are 74% for Sample A and 37% only for Sample B which shows that the Sample A appears to be more amenable to beneficiate. A concentrate of 46.7% with 63.22% Fe could be recovered from Sample A while subjected to gravity separation followed by wet magnetic separation. The Sample B does not respond to gravity and magnetic separation due to its complex mineralogy. However, calcination of the Sample B followed by magnetic separation gives the encouraging results. Thus, anomalous behaviour of the goethite dominated ores in beneficiation is attributed to the different textural and liberation characteristic.

关键词： Texture Pisolitic Ochreous goethite Vitreous goethite Calcination

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An Improved Methodology to Overcome key Issues in Human Fecal Metagenomic DNA Extraction

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Genomics, Proteomics & Bioinformatics 2016年第6期14卷 371-378页

作者： Jitendra kumar manoj kumar Shashank Gupta Vasim Ahmed Manu Bhambi Rajesh Pandey Nat Singh Chauhan Department of Biochemistry Maharshi Dayanand UniversityRohtak 124001India Ayurgenomics Unit Translational Research and Innovative Science Through AyurgeomicsCouncil of Scientific and Industrial ResearchInstitute of Genomics and Integrative BiologyNew Delhi 110020India

Microbes are ubiquitously distributed in nature, and recent culture-independent studies have highlighted the significance of gut microbiota in human health and disease. Fecal DNA is the primary source for the majority of human gut microbiome studies. However, further improvement is needed to obtain fecal metagenomic DNA with sufficient amount and good quality but low host genomic DNA contamination. In the current study, we demonstrate a quick, robust, unbiased, and cost-effective method for the isolation of high molecular weight （〉 23 kb） metagenomic DNA （260/280 ratio 〉 1.8） with a good yield （55.8 ± 3.8 ng/mg of feces）. We also confirm that there is very low human genomic DNA contamination （eubacterial： human genomic DNA marker genes = 227.9：1） in the human feces. The newly-developed method robustly performs for fresh as well as stored fecal samples as demonstrated by 16S rRNA gene sequencing using 454 FLX＋. Moreover, 16S rRNA gene analysis indicated that compared to other DNA extraction methods tested, the fecal metagenomic DNA isolated with current methodology retains species richness and does not show microbial diversity biases, which is further confirmed by qPCR with a known quantity of spike-in genomes. Overall, our data highlight a protocol with a balance between quality, amount, user-friendliness, and cost effectiveness for its suitability toward usage for cultureindependent analysis of the human gut microbiome, which provides a robust solution to overcome key issues associated with fecal metagenomic DNA isolation in human gut microbiome studies.

关键词： Metagenomic DNAextraction Gut microbiome Human feces 16S rRNA

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