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Effect of Probiotics on Juvenile Tachypleus tridentatus Gut Microbiota

Journal of Ocean University of China 2022年第3期21卷 564-572页

作者： xiong kai MIAO Fengze WAIHO khor lIU Ximei FANG James k.H. HE Jinfeng HU Menghong WANG Youji key laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai Ocean UniversityShanghai201306China Higher Institution Center of Excellence(HICoE) Institute of Tropical Aquaculture and FisheriesUniversity Malaysia TerengganuKuala TerengganuTerengganu20000Malaysia Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic UniversityHung HomKowloon999077China Beihai Product Quality Testing Institute Beihai536000China

The population of tri-spine horseshoe crabs(HSCs)Tachypleus tridentatus has sharply decreased in recent *** and manual releasing are now used to recover *** order to accelerate the growth of *** in indoor environment,in this study,gut microbiota of 2nd instar *** was supposed to be improved by putting probiotics in water environment and thus promoted the health and growth of the *** 16S rRNA sequencing of gut samples,we examined the intestinal microbiome of juvenile HSCs and studied the effect of *** showed that there was no significant difference between groups fed with high and low concentrations(1×10^(7),1×10^(12)CFU ml^(−1))of Bacillus subtilis and high concentration(1×10^(12)CFU ml^(−1))of lactobacillus,and no significant difference compared with the control group,but the diversity of lactobacillus group with low concentration(1×10^(7)CFU ml^(−1))was significantly higher than those of other ***-mediated functions predicted by PICRUSt showed that‘Cellular Processes’,‘Metabolism’and‘Organismal Systems’pathways significantly increased in groups of Bacillus subtilis and lactobacillus group with low *** results showed that two types of probiotics with different concentrations have other effects on the structure,richness and function of intestinal *** searching the literature and conducting pre-experiments,we forecasted the short time of breeding under the probiotic environment may not have much *** present study for the first time investigated the impact of probiotics on gut microbiota of juvenile HSCs and found them may benefit for the growth and health of HSCs.

关键词： Tachypleus tridentatus probiotic gut microbiota diversity richness

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Biocompatible and non cytotoxic nucleoside-based AIEgens sensor for lighting-up nucleic acids

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Chinese Chemical letters 2021年第5期32卷 1687-1690页

作者： Qiuyun Xiao Xuan Zhao Hai xiong Institute for Advanced Study Shenzhen UniversityShenzhen 518060China key laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Optoelectronic EngineeringShenzhen UniversityShenzhen 518060China

Aggregation-induced emission luminogens(AIEgens) have been used in biomacromolecules ***,TPE-dC and TPE-dU acted as the nucleoside-based AIEgens sensors in the first case,which can be used to detect ctDNA and rRNA in vitro and light up the nucleus in vivo depending on the intermolecular binding *** AIE process enables the quantitative analysis or visualization of nucleic acids in solution or gels state,***,confocal laser scanning microscopy(ClSM) images of l929 cells stained with TPE-dC or TPE-dU clearly shows that nucleoside-based AIEgens bio-probes can pass the cell membranes to reach the cell nucleus,without cytotoxicity at the imaging condition(incubation time> 12 h,and 10 μmol/l of concentration).Since the nucleus is rich in DNA/RNA,fluorescence turn-on mode has a great potential in nucleus imaging and clinical diagnosis.

关键词： AIEgens Nucleoside Biosensor Nucleus localization Cell imaging

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Silver sulfide leaching with a copper-thiosulfate solution in the absence of ammonia

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Rare Metals 2011年第2期30卷 105-109页

作者： CUI Yiqi TONG xiong Alejandro lopez-Valdivieso Faculty of land and Resource Engineering Kunming University of Science and Technology Kunming 650093 China Instituto de Metalurgia UniversidadAutonoma de San Luis Potosi San Luis Potosi C.P. 78210 Mexico

leaching from silver *** a copper-thiosulfate solution in the absence of ammonia was investigated. It is found that silver sulfide could be effectively dissolved in the copper-thiosulfate system without ammonia. The effects of thiosulfate concentration, cop- per-to-thiosulfate ratio, agitation vigorousness, pH values, and dissolved gases on the leaching of silver sulfide with the copper-thiosulfate solution were studied. A maximum extraction of 95.1% was achieved with the 0.12 mol/l thiosulfate and the 0.048 rnol/l copper sulfate. It is shown that copper-to-thiosulfate ratio is an important factor in silver sulfide dissolution and oxygen is not needed to leach silver sulfide, which leads to the instability of the leaching system at high copper-to-thiosulfate ratio. On the other hand, the silver extraction is not affected by the pulp pH values.

关键词： extraction silver leaching copper sulfate thiosulfate

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Adsorption behavior of Pd(Ⅱ) from aqueous solutions by D201 resin

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Rare Metals 2011年第5期30卷 470-476页

作者： xiong Chunhua ZHU Shan YAO Caiping CHEN Qing Department of Applied Chemistry Zhejiang Gongshang University Hangzhou 310012 China

The adsorption and desorption behaviors of Pd（Ⅱ） on D201 resin were investigated. The effects of parameters including HCl concentrations, initial metal ion concentrations, contact time, and temperature on the adsorption of Pd（Ⅱ） were studied by batch method. The HCl concentration for studying the effects was set from 0.01 to 2 mol·l-1 and the optimal adsorption condition is in 0.01 mol·l-1 HCl solution. The adsorption isotherm of Pd（Ⅱ） on D201 resin was determined and the equilibrium data fitted the langmuir model better than the Freundlich model. The maximum uptake capacity of Pd（Ⅱ） was 253 mg·g-1 at 298 k. The adsorption could be best described by the liquid film diffusion model. The enthalpy variation （ΔH） and entropy change （ΔS） of adsorption were equal to 2.49 kJ·mol-1 and 74.8 J·（mol·k）-1 respectively, indicating that the adsorption was endothermic in nature. Furthermore, the negative value of ΔG showed that movement of adsorbate from the solution to adsorbent surface was a spontaneous process. Pd（Ⅱ） could be eluted with 0.5 mol·l-1 HNO 3 solution. Thomas model was applied to experimental column data to determine the characteristic parameters of the column useful for process design.

关键词： adsorption palladium resins thermodynamics kinetics

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Edge reconstruction of layer-dependentβ-In2Se3/MoS2 vertical heterostructures for accelerated hydrogen evolution

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Nano Research 2023年第1期16卷 1670-1678页

作者： Gonglei Shao Meiqing Yang Haiyan Xiang Song luo xiong-xiong Xue Huimin li Xu Zhang Song liu Zhen Zhou Engineering Research Center of Advanced Functional Material Manufacturing of Ministry of Education School of Chemical EngineeringZhengzhou UniversityZhengzhou 450001China Institute of Chemical Biology and Nanomedicine(ICBN) State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China College of life and Environmental Science Hunan University of Arts and ScienceChangde 415000China School of Physics and Optoelectronics Xiangtan UniversityXiangtan 411105China Institute of New Energy Material Chemistry School of Materials Science and EngineeringNankai UniversityTianjin 300350China

The layer-dependent properties are still unclarified in two-dimensional(2D)vertical *** this study,we layer-bylayer deposited semimetalβ-In2Se3 on monolayer MoS2 to form verticalβ-In2Se3/MoS2 heterostructures by chemical vapor *** defect-mediated nucleation mechanism inducesβ-In2Se3 nanosheets to grow on monolayer MoS2,and the layer number of stackedβ-In2Se3 can be precisely regulated from 1 layer(l)to 13 l by prolonging the growth ***β-In2Se3/MoS2 heterostructures reveal tunable type-Ⅱband alignment arrangement by altering the layer number ofβ-In2Se3,which optimizes the internal electron ***,the edge atomic structure ofβ-In2Se3 stacking on monolayer MoS2 shows the reconstruction derived from large lattice mismatch(~29%),and the presence ofβ-In2Se3 also further increases the electrical conductivity ofβ-In2Se3/MoS2 *** to abundant layer-dependent edge active sites,edge reconstruction,improved hydrophilicity,and high electrical conductivity ofβ-In2Se3/MoS2 heterostructures,the edge ofβ-In2Se3/MoS2 heterostructures exhibits excellent electrocatalytic hydrogen evolution *** onset potential and smaller Tafel slope can be observed at the edge of monolayer MoS2 coupled with 13-lβ-***,the outstanding conductive layers coupled with edge reconstruction in 2D vertical heterostructures play decisive roles in the optimization of electron energy levels and improvement of layer-dependent catalytic performance.

关键词： In2Se3/MoS2 heterostructure edge reconstruction layer dependent hydrogen evolution reaction microreactor

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Removal of hexavalent chromium from contaminated waters by ultrasound-assisted aqueous solution ball milling

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Journal of Environmental Sciences 2017年第2期29卷 276-283页

作者： lin Chen Zhenhua Chen Ding Chen Wei xiong College of Materials Science and Engineering Hunan UniversityChangsha 410082China

Batch mode experiments were conducted to study the removal of hexavalent chromium（Cr（Ⅵ）） from aqueous solutions using ultrasound-assisted aqueous solution ball *** results show that the reduction rate of Cr（Ⅵ） by ultrasound-assisted aqueous solution ball milling was significantly faster than that by ball milling or ultrasound treatment alone,and an initial Cr（Ⅵ） concentration of 166 mg/l could be decreased to 0.35 mg/l at 120 *** decisive factors, including initial concentration of Cr（Ⅵ）, p H value, ultrasonic frequency and filling gas, were studied. It was found that the optimal ultrasonic frequency for ultrasound-assisted aqueous solution ball milling device was 20 k Hz, and the rate of Cr（Ⅵ）reduction as a function of filling gas followed the order： Ar 〉 air 〉 N2〉 O2. Samples were characterized by X-ray diffraction, fluorescence measurements, atomic absorption and the diphenylcarbazide colorimetric method. The Cr（Ⅵ） transformed into a precipitate that could be removed from the contaminated water, after which the water could be reused.

关键词： Cr(VI) Ultrasound wave Ball milling Hydroxyl radicals

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Effects of culture conditions on ligninolytic enzymes and protease production by Phanerochaete chrysosporium in air

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Journal of Environmental Sciences 2008年第1期20卷 94-100页

作者： xiong Xiaoping WEN Xianghua BAI Yanan QIAN Yi State key Joint laboratory of Environmental Simulation and Pollution Control Department of Environmental Science and Engineering Tsinghua University Beifing 100084 China.

The production of ligninolytic enzymes and protease by Phanerochaete chrysosporium was investigated under different culture conditions. Different amounts of medium were employed in free and immobilized culture, together with two kinds of medium with different C/N ratios. little lignin peroxidase （lIP）（〈 2 U/l） was detected in free culture with nitrogen-limited medium （C/N ratio： 56/2.2, in mmol/l）, while manganese peroxidase （MnP） maximum activity was 231 and 240 U/l in 50 and 100 ml medium culture, respectively. Immobilized culture with 50 ml nitrogen-limited medium gave the highest MnP and liP production with the maximum values of 410 and 721 U/l separately on the day 5; however, flasks containing 100 ml nitrogen-limited medium only produced less MnP with a peak value of 290 U/l. Comparatively, carbon-limited medium （C/N ratio： 28/44, in mmol/l） was adopted in culture but produced little MnP and liE Medium type had the greatest impact on protease production. large amount of protease was produced due to glucose limitation. Culture type and medium volume influence protease activity corporately by affecting oxygen supply. The results implied shallow immobilized culture was a possible way to gain high production of ligninolytic enzymes.

关键词： protease culture conditions ligninolytic enzymes Phanerochaete chrysosporiurn

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Male larval experience of cues from adult rivals alters lifetime sperm investment patterns in a sperm heteromorphic moth, Ephestia kuehniella

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Insect Science 2023年第6期30卷 1773-1783页

作者： Junyan liu xiong Zhao He Qiao Wang School of Agriculture and Environment Massey UniversityPalmerston NorthNew Zealand

Male animals may adjust their resource allocations for reproduction and other fitness functions in response to cues from rivals. For instance, adult males increase their investment in sperm for a higher paternity share when they perceive sperm competition risk in their surroundings. In nature, both juveniles and adults may coexist spatially and temporally. Yet, it is not clear how juvenile males of different ages respond to cues from adult rivals and fine-tune their lifetime investment in sperm production and ejaculation in any insect. Here we used the Mediterranean flour moth, Ephestia kuehniella, which produces both fertile eupyrene and infertile apyrene sperm, to explore this question. We demonstrate that the late, but not early, instar larvae are sensitive to adult male cues. As a response, they produce more sperm before emergence and their resultant adults have shorter mating latency and ejaculate more sperm in the first few matings. When the juvenile stage produces more eupyrenes, the adult stops making these sperm, but regardless of the number of apyrenes produced during the juvenile stage, the adult continues to make them. These findings suggest that the number of spermatogonia for eupyrenes may be limited and that for apyrenes may be flexible. Our results show that the insect does not trade off survival, mating frequency, body size, or testis size for sperm production in response to adult males during the larval stage. knowledge created in the present study offers insight into the stage-dependent sensitivity of juvenile males to cues from adult rivals and subsequent lifetime resource allocations.

关键词： immature stage lepidoptera spermatogenesis sperm allocation sociosex-ual environment

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Seaweed glue as a novel polymer depressant for the selective separation of chalcopyrite and galena

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International Journal of Minerals,Metallurgy and Materials 2019年第12期26卷 1495-1503页

作者： xiong Chen Guo-hua Gu Zhi-xiang Chen School of Minerals Processing and Bioengineering Central South University

The use of seaweed glue(SEG)as a novel polymer depressant for the separation of chalcopyrite from galena with butyl xanthate(BX)as a collector was studied through microflotation *** angle,adsorption,dynamic potential,and infrared spectral analyses were conducted to clarify the mechanism underlying the action of SEG on *** results of microflotation experiments indicated that chalcopyrite could be selectively separated from galena by using a SEG depressant concentration of 15 mg·l^-1,BX concentration of 10 mg·l^-1,and methyl isobutyl carbinol concentration of 8.5 mg·l^-1 at pH 8.0.A Cu concentrate with a grading of 23.68wt% was obtained at a recovery rate of 81.52% from mixed minerals with 8.29wt% Cu *** angle analysis showed that the effect of SEG on the wettability of galena was stronger than that on the wettability of ***,zeta potential,and FT-IR spectral analyses revealed that SEG and BX were coadsorbed on the surfaces of *** depressed galena by covering xanthate ions in the functional groups of-COO and mainly underwent weak physisorption on *** mechanisms account for the ability of SEG to depress galena effectively while enabling chalcopyrite flotation.

关键词： seaweed glue chalcopyrite galena flotation separation

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Effect of reclaimed water effluent on bacterial community structure in the Typha angustifolia l. rhizosphere soil of urbanized riverside wetland, China

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Journal of Environmental Sciences 2017年第5期29卷 58-68页

作者： Xingru Huang Wei xiong Wei liu Xiaoyu Guo College of Resources Environment and Tourism Capital Normal University Beijing 100048 China Belting Municipal key laboratory of Resources Environment and GIS Beijing 100048 China Urban Environmental Processes and Digital Modeling laboratory Beijing 100048 China Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China

In order to evaluate the impact of reclaimed water on the ecology of bacterial communities in the Typha angustifolia l. rhizosphere soil, bacterial community structure was investigated using a combination of terminal restriction fragment length polymorphism and 16S rRNA gene clone library. The results revealed significant spatial variation of bacterial communities along the river from upstream and downstream. For example, a higher relative abundance of γ-Proteobacteria, Firmicutes, Chloroflexi and a lower proportion of β-Proteobacteria and ε-Proteobacteria was detected at the downstream site compared to the upstream site. Additionally, with an increase of the reclaimed water interference intensity, the rhizosphere bacterial community showed a decrease in taxon richness, evenness and diversity. The relative abundance of bacteria closely related to the resistant of heavy-metal was markedly increased, while the bacteria related for carbon/nitrogen/phosphorus/sulfur cycling wasn＇t strikingly changed. Besides that, the pathogenic bacteria markedly increased in the downstream rhizosphere soil since reclaimed water supplement, while the possible plant growth-promoting rhizobacteria obviously reduced in the downstream sediment. Together these data suggest cause and effect between reclaimed water input into the wetland, shift in bacterial communities through habitat change, and alteration of capacity for biogeochemical cycling of contaminants.

关键词： Reclaimed water T-RFlP 16S rRNA gene clone library Bacterial community structure

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