Effect of Probiotics on Juvenile Tachypleus tridentatus Gut Microbiota

作     者：XIONG Kai MIAO Fengze WAIHO Khor LIU Ximei FANG James K.H. HE Jinfeng HU Menghong WANG Youji XIONG Kai;MIAO Fengze;WAIHO Khor;LIU Ximei;FANG James K.H.;HE Jinfeng;HU Menghong;WANG Youji

作者机构：Key Laboratory of Exploration and Utilization of Aquatic Genetic ResourcesMinistry of EducationShanghai Ocean UniversityShanghai201306China Shanghai Collaborative Innovation for Aquatic Animal Genetics and BreedingShanghai Ocean UniversityShanghai201306China Higher Institution Center of Excellence(HICoE)Institute of Tropical Aquaculture and FisheriesUniversity Malaysia TerengganuKuala TerengganuTerengganu20000Malaysia Department of Applied Biology and Chemical TechnologyThe Hong Kong Polytechnic UniversityHung HomKowloon999077China Beihai Product Quality Testing InstituteBeihai536000China 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2022年第21卷第3期

页      面：564-572页

核心收录：

学科分类：0908[农学-水产] 0707[理学-海洋科学] 09[农学] 

基　　金：the National Natural Science Foundation of China(No.31872587) the CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation the YICCAS Grant(No.2020 KFJJ11) 

主　　题：Tachypleus tridentatus probiotic gut microbiota diversity richness 

摘      要：The population of tri-spine horseshoe crabs(HSCs)Tachypleus tridentatus has sharply decreased in recent *** and manual releasing are now used to recover *** order to accelerate the growth of *** in indoor environment,in this study,gut microbiota of 2nd instar *** was supposed to be improved by putting probiotics in water environment and thus promoted the health and growth of the *** 16S rRNA sequencing of gut samples,we examined the intestinal microbiome of juvenile HSCs and studied the effect of *** showed that there was no significant difference between groups fed with high and low concentrations(1×10^(7),1×10^(12)CFU mL^(−1))of Bacillus subtilis and high concentration(1×10^(12)CFU mL^(−1))of Lactobacillus,and no significant difference compared with the control group,but the diversity of Lactobacillus group with low concentration(1×10^(7)CFU mL^(−1))was significantly higher than those of other ***-mediated functions predicted by PICRUSt showed that‘Cellular Processes’,‘Metabolism’and‘Organismal Systems’pathways significantly increased in groups of Bacillus subtilis and Lactobacillus group with low *** results showed that two types of probiotics with different concentrations have other effects on the structure,richness and function of intestinal *** searching the literature and conducting pre-experiments,we forecasted the short time of breeding under the probiotic environment may not have much *** present study for the first time investigated the impact of probiotics on gut microbiota of juvenile HSCs and found them may benefit for the growth and health of HSCs.