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Effects of chlortetracycline,Cu and their combination on the performance and microbial community dynamics in swine manure anaerobic digestion

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Journal of Environmental Sciences 2018年第5期30卷 206-215页

作者： Rui Wang junya Zhang Jibao Liu Dawei Yu Hui Zhong Yawei Wang Meixue Chen Juan Tong Yuansong Wei State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China. Institute of Energy Jiangxi Academy of SciencesNanchang 330096China University of Chinese Academy of Sciences Beijing 100049China

Swine manure was typical for the combined pollution of heavy metals and antibiotics. The effects of widely used veterinary antibiotic chlortetracycline（CTC）, Cu and their combination on swine manure anaerobic digestion performance and microbial community have never been investigated. Thus, four 2 L anaerobic digestion reactors were established including reactor A（control）, B（CTC spiked by 0.5 g/kg dry weight, dw）, C（Cu spiked by 5 g/kg dw） and D（combination of CTC, 0.5 g/kg dw, and Cu, 5 g/kg dw）, and dynamics of bacterial and archaeal community structure was investigated using high throughput sequencing method. Results showed that addition of CTC and Cu separately could increase the total biogas production by21.6% and 15.8%, respectively, while combination of CTC and Cu severely inhibited anaerobic digestion（by 30.3%）. Furthermore, corresponding to different stages and reactors, four kinds of microbes including bacteria and archaea were described in detail, and the effects of CTC, Cu and their combination mainly occurred at hydrolysis and acidification phases. The addition of Cu alone changed the dynamics of archaeal community significantly. It was genus Methanomassiliicoccus that dominated at the active methane production for A, B and D, while it was genus Methanobrevibacter and Methanoculleus for C.

关键词： Chlortetracycline Cu Anaerobic digestion Swine manure Microbial community

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The Role of Nonlinear Forcing Singular Vector Tendency Error in Causing the"Spring Predictability Barrier"for ENSO

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Journal of Meteorological Research 2016年第6期30卷 853-866页

作者： DUAN Wansuo ZHAO Peng2 HU junya1 XU Hui1 State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics Institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China Meteorological Administration Training Center Beijing 100081 University of Chinese Academy of Sciences Beijing 100049

With the Zebiak-Cane model, the present study investigates the role of model errors represented by the nonlinear forcing singular vector （NFSV） in the ＂spring predictability barrier＂（SPB） phenomenon in ENSO prediction. The NFSV-related model errors are found to have the largest negative effect on the uncertainties of El Nino prediction and they can be classified into two types： the first is featured with a zonal dipolar pattern of SST anomalies （SSTA）, with the western poles centered in the equatorial central western Pacific exhibiting positive anomalies and the eastern poles in the equatorial eastern Pacific exhibiting negative anomalies; and the second is characterized by a pattern almost opposite to the first type. The first type of error tends to have the worst effects on El Nifio growth-phase predictions, whereas the latter often yields the largest negative effects on decaying-phase predictions. The evolution of prediction errors caused by NFSV- related errors exhibits prominent seasonality, with the fastest error growth in spring and/or summer; hence, these errors result in a significant SPB related to El Nifio events. The linear counterpart of NFSVs, the （linear） forcing singular vector （FSV）, induces a less significant SPB because it contains smaller prediction errors. Random errors cannot generate an SPB for El Nifio events. These results show that the occurrence of an SPB is related to the spatial patterns of tendency errors. The NFSV tendency errors cause the most significant SPB for El Nifio events. In addition, NFSVs often concentrate these large value errors in a few areas within the equatorial eastern and central-western Pacific, which likely represent those areas sensitive to El Nifio predictions associated with model errors. Meanwhile, these areas are also exactly consistent with the sensitive areas related to initial errors determined by previous studies. This implies that additional observations in the sensitive areas would not only improve the a

关键词： spring predictability barrier model error optimal perturbation El Nifio event

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Newly designed J-shaped tip guidewire: A preliminary feasibility study in wire-guided cannulation

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World Journal of Gastroenterology 2013年第28期19卷 4531-4536页

作者： Shigefumi Omuta Iruru Maetani Hiroaki Shigoka Katsushige Gon Michihiro Saito junya Tokuhisa Mieko Naruki Division of Gastroenterology Department of Internal Medicine Toho University Ohashi Medical Center Tokyo 153-8515 Japan

AIM: To perform wire-guided cannulation using a newly designed J-shaped tip guidewire, and to verify feasibility and safety for use. METHODS: The study was conducted on endoscopic retrograde cholangiopancreatography (ERCP) patients with na ve papilla undergoing diagnosis and treatment of biliary diseases between September 2011 and July 2012. We performed ERCP in a succession of 50 cases with a J-shaped tip guidewire. The first insertion attempt began with a trainee who had 5 min to complete cannulation, followed if necessary by the trainer for another 5 min. We assessed the primary success rate of selective biliary cannulation within 10 min and adverse events such as post-ERCP pancreatitis (PEP), bleeding or ***: The primary success rate was 90% (45/50) within 10 min, the initial success rate within 5 min by trainee staff was 76% (38/50). The rate of PEP was 6% (3/50), but all 3 cases were mild pancreatitis. All patients were managed successfully with conservative treatment. There was no bleeding or perforation. CONCLUSION: A newly designed J-shaped tip guidewire has the possibility to facilitate selective biliary cannulation for ERCP and appears to be safe.

关键词： J-shaped tip guidewire Wire-guided cannulation Endoscopic retrograde cholangiopancreatography Biliary tract Cannulation technique Perforation

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Delaminated layered double hydroxide delivers DNA molecules as sandwich nanostructure into cells via a non-endocytic pathway

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Science Bulletin 2017年第10期62卷 686-692页

作者： Ye Li Wenlong Bao Hongyang Wu junya Wang Yu Zhang Yinglang Wan Dapeng Cao Dermot O'Hare Qiang Wang College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 China Faculty of Environmental Science and Engineering Kunming University of Science and Technology Kunming 650500 China College of Environmental Science and Engineering Beijing Forestry University Beijing 100083 China State Key Laboratory of Organic-Inorganic Composites Beijing University of Chemical Technology Beijing 100029 China Chemistry Research Laboratory Department of Chemistry University of Oxford Oxford OX1 3TA UK

Layered double hydroxides （LDHs） are effective molecular carriers in cytological research, gene therapy, and transgenic applications. Herein, we investigated the internalization behavior of the LDH-DNA biocon- jugates via a microscopic approach and analyzed the internalization pathway by dissipative particle dynamics （DPD） simulations. We experimentally found that LDH can efficiently carry DNA into the nucleus of cell in BY-2 suspension cells. Furthermore, atomic force microscopy and X-ray diffraction anal- ysis demonstrated that the LDH-DNA bioconjugates mainly exist as a DNA-LDH-DNA sandwich complex, while the LDH-DNA-LDH sandwich complex and DNA-LDH complex cannot be excluded. The DPD simu- lations further indicated that only the DNA-LDH-DNA sandwich structure could penetrate the plasma membrane （PM）, while PM is impermeable to the LDH-DNA-LDH sandwich complex and the DNA-LDH complex. This work provides novel perspective for understanding the membrane penetration mechanism of LDH nano-sheets and new insights into the design of novel molecular delivery systems.

关键词： Layered double hydroxides DNA Gene deliveryEffective penetration structure Dissipative particle dynamics simulations

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Environmental Stability and Transmissibility of Enveloped Viruses at Varied Animate and Inanimate Interfaces

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Environment & Health 2023年第1期1卷 15-31页

作者： Li Zeng junya Li Meilin Lv Zikang Li Linlin Yao Jie Gao Qi Wu Ziniu Wang Xinyue yang Gang Tang Guangbo Qu Guibin Jiang State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China College of Sciences Northeastern UniversityShenyang 110819China School of Environment Hangzhou Institute for Advanced StudyUCASHangzhou 310000China Institute of Environment and Health Jianghan UniversityWuhan 430056China

Enveloped viruses have been the leading causative agents of viral epidemics in the past decade,including the ongoing coronavirus disease 2019 *** epidemics caused by enveloped viruses,direct contact is a common route of infection,while indirect transmissions through the environment also contribute to the spread of the disease,although their significance remains *** the knowledge gap regarding the influence of interfacial interactions on the persistence of enveloped viruses in the environment reveals the transmission mechanisms when the virus undergoes mutations and prevents excessive disinfection during viral ***,from the perspective of the driving force,partition efficiency,and viral survivability at interfaces,we summarize the viral and environmental characteristics that affect the environmental transmission of *** expect to provide insights for virus detection,environmental surveillance,and disinfection to limit the spread of severe acute respiratory syndrome coronavirus 2.

关键词： enveloped virus environmental transmission interface survivability SARS-CoV-2

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Targeted B-domain deletion restores F8 function in human endothelial cells and mice

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Signal Transduction and Targeted Therapy 2022年第7期7卷 2148-2150页

作者： Zhiqing Hu Zhuo Li Yong Wu junya Zhao Lingqian Wu Miaojin Zhou Desheng Liang Center for Medical Genetics School of Life SciencesCentral South UniversityChangshaHunan 410078China

Dear Editor,Hemophilia A(HA)is the most common genetic bleeding disorders and affects 1 in 5000 male births.1 HA patients suffer spontaneous soft-tissue and joint bleeding or life-threatening intracranial *** main clinical treatment for severe HA patients is life-long maintenance of protein replacement therapy,which increase the risks of infection and induce FVIII *** therapy is expected to cure HA,however,the current AAVbased gene addition strategy is limited by its loading capacity,immunogenicity of capsids,and the risk of insertional mutagenesis.2 Therefore,the aim of this study is to overcome these limitations by a combination of gene editing and ex vivo strategy for HA gene therapy.

关键词： overcome insertion function

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Study on Spray Characteristics of Dimethyl Carbonate Blended Fuel in IC Engines

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China Petroleum Processing & Petrochemical Technology 2021年第4期23卷 29-36页

作者： Fu Wei Zhou Kun Liu junya Xie Hongxi Li Yayun Lu Lei Bao Jihu Cheng Yunlang Hefei General Machinery Research Institute Hefei 230031

The spray characteristics of the blended fuel composed of biodiesel and dimethyl carbonate were compared with those of biodiesel and *** macroscopic spray characteristic parameters including spray tip penetration,maximum spray width,average spray cone angle,and peak tip velocity were used to evaluate the spray characteristics of the blended *** results showed that the spray tip penetration of blended fuel was lower than that of biodiesel,while the spray cone angle and maximum spray width of blended fuel were both larger than that of *** the basis of the Hiroyasu and Arai mathematical model combined with the actual working conditions of this experiment,including physical parameters such as the viscosity and density of the fuel,a correct model of spray penetration was established,and the experimental results were compared.

关键词： biodiesel dimethyl carbonate diesel spray characteristics mathematical model

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A Novel Flux-weakening Control Method with Quadrature Voltage Constrain for Electrolytic Capacitorless PMSM Drives

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CES Transactions on Electrical Machines and Systems 2022年第3期6卷 279-287页

作者： junya Huo Dawei Ding Zekun Ren Gaolin Wang Nannan Zhao Lianghong Zhu Dianguo Xu the School of Electrical Engineering and Automation Harbin Institute of TechnologyHarbin 150001China the Guangdong Midea Air-Conditioning Equipment Co. Ltd.Foshan 528311China IEEE

In electrolytic capacitorless permanent magnet synchronous motor(PMSM) drives, the DC-link voltage will fluctuate in a wide range due to the use of slim film capacitor. When the flux-weakening current is lower than-ψf/Ld during the high speed operation, the flux-weakening control loop will transform to a positive feedback mode, which means the reduction of flux-weakening current will lead to the acceleration of the voltage saturation, thus the whole system will be unstable. In order to solve this issue, this paper proposes a novel flux-weakening method for electrolytic capacitorless motor drives to maintain a negative feedback characteristic of the control loop during high speed operation. Based on the analysis of the instability mechanism in flux-weakening region, a quadrature voltage constrain mechanism is constructed to stabilize the ***, the parameters of the controller are theoretically designed for easier industrial application. The proposed algorithm is implemented on a 1.5 kW electrolytic capacitorless PMSM drive to verify the effectiveness of the flux-weakening performance.

关键词： Permanent magnet synchronous motor(PMSM) Electrolytic capacitorless drive Flux-weakening control Voltage constrain Stable operation

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Numerical Evaluation of the Surface Energy of Polyhedral Nanoparticles

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Materials Sciences and Applications 2020年第12期11卷 837-850页

作者： Takuya Uehara junya Fujiwara Department of Mechanical Systems Engineering Yamagata University Yonezawa Japan Graduate School of Science and Engineering Yamagata University Yonezawa Japan

Nanoparticles have been used widely in various fields, and their size and shape greatly affect the functional properties. Therefore, controlling the morphology of the particles is important, and evaluation of the surface energy is indispensable for that purpose. In this study, the surface energy of nanoparticles was evaluated by numerical simulation and formulated in a polynomial equation. First, molecular dynamics simulations were carried out for variously shaped polyhedral nanoparticles. A cube and an octahedron were introduced as reference shapes, and truncated hexahedrons and truncated octahedrons were created by cutting out their vertices. The surface energy was plotted for various polyhedrons. The lowest energy was observed in an octahedron because of the stability of the (111) plane, and the highest energy was observed in a cube because of the relatively higher energy of the (100) plane. Then, the surface energy was formulated in a polynomial equation, in which the parameters obtained by the molecular-dynamics simulations were introduced. As a result, stability of the octahedron and relative instability of the cube were fairly captured by the proposed polynomial equation, while a slight underestimation was inevitable. Finally, the parameters were revised to continuous numbers to extend the application range. Consequently, an application for various materials, such as a cube having equivalent stability to an octahedron, was demonstrated by imposing rather exaggerated parameters.

关键词： Surface Energy Nanoparticle Fcc Crystal Interatomic Potential Molecular Dynamics Computer Simulation

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Characteristics and prognosis of synchronous multiple early gastric cancer

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World Journal of Gastroenterology 2013年第41期19卷 7154-7159页

作者： Taro Isobe Kousuke Hashimoto junya Kizaki Naotaka Murakami Keishiro Aoyagi Kikuo Koufuji Yoshito Akagi Kazuo Shirouzu Department of Surgery Kurume University School of MedicineFukuoka 830-0011Japan

AIM:To assess the clinicopathologic characteristics,risk factors,and prognosis for synchronous multiple early gastric cancer(SMGC).METHODS:A total of 146 patients with SMGC and1194 patients with single gastric cancer who had undergone gastrectomy between 1989 and 2008 were retrospectively analyzed to determine their clinicopathologic characteristics and postoperative *** were classified into groups on the basis of location and *** habits were evaluated using the Brinkman *** and pathological factors were compared using either Fisher’s exact test or Pearson’sχ*** regression analysis was performed to identify independent risk *** rate was calculated using the Kaplan-Meier ***:SMGCs accounted for 10.9%of gastric cancer cases and occurred predominantly in elderly male patients with a family history of gastric cancer who were both smokers and *** tumors were typically macroscopically elevated and histologically *** were no significant differences between SMGC and single gastric cancer patients with respect to tumor location,tumor size,lymph node metastasis,the number of metastatic lymph nodes,venous invasion,or tumor stage(P=0.052,P=0.347,P=0.595,P=0.805,P=0.559,and P=0.408,respectively).Further,there was no significant difference in postoperative survival between the patient groups(P=0.200).Of the 146SMGC patients,a single patient had remnant ***:A careful preoperative endoscopy is necessary for patients who are at high risk of SMGC,and minimally invasive treatment may be indicated in some cases.

关键词： Gastric cancer Synchronous Multiple Endoscopy Prognosis

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