Effects of chlortetracycline,Cu and their combination on the performance and microbial community dynamics in swine manure anaerobic digestion

作     者：Rui Wang Junya Zhang Jibao Liu Dawei Yu Hui Zhong Yawei Wang Meixue Chen Juan Tong Yuansong Wei 

作者机构：State Key Laboratory of Environmental Aquatic ChemistryResearch Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China. Institute of EnergyJiangxi Academy of SciencesNanchang 330096China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2018年第30卷第5期

页      面：206-215页

核心收录：

学科分类：082803[工学-农业生物环境与能源工程] 07[理学] 08[工学] 0828[工学-农业工程] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China(Nos.21377151,21577161 and 21677161) the State Key Joint Laboratory of Environmental Simulation and Pollution Control of China(No.16Z04ESPCR) the National Major Science&Technology Projects for Water Pollution Control and Management of China(No.2015ZX07203-007) the National Key R&D program(No.2016YFD0501405) 

主　　题：Chlortetracycline Cu Anaerobic digestion Swine manure Microbial community 

摘      要：Swine manure was typical for the combined pollution of heavy metals and antibiotics. The effects of widely used veterinary antibiotic chlortetracycline（CTC）, Cu and their combination on swine manure anaerobic digestion performance and microbial community have never been investigated. Thus, four 2 L anaerobic digestion reactors were established including reactor A（control）, B（CTC spiked by 0.5 g/kg dry weight, dw）, C（Cu spiked by 5 g/kg dw） and D（combination of CTC, 0.5 g/kg dw, and Cu, 5 g/kg dw）, and dynamics of bacterial and archaeal community structure was investigated using high throughput sequencing method. Results showed that addition of CTC and Cu separately could increase the total biogas production by21.6% and 15.8%, respectively, while combination of CTC and Cu severely inhibited anaerobic digestion（by 30.3%）. Furthermore, corresponding to different stages and reactors, four kinds of microbes including bacteria and archaea were described in detail, and the effects of CTC, Cu and their combination mainly occurred at hydrolysis and acidification phases. The addition of Cu alone changed the dynamics of archaeal community significantly. It was genus Methanomassiliicoccus that dominated at the active methane production for A, B and D, while it was genus Methanobrevibacter and Methanoculleus for C.