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Chinese Chemical Letters 2022年第9期33卷 4175-4178页

作者： junping bai Junliang Zhou Xin Ji Nannan Wang Xiaochun Dong Wei Wu Weili Zhao School of Pharmacy Institutes of Integrative MedicineFudan UniversityShanghai 201203China Key Laboratory for Special Functional Materials of Ministry of Education School of Materials Science and EngineeringHenan UniversityKaifeng 475004China

Fluorescent dyes play a crucial role in fluorescence imaging and sensing technology.However,there is a dilemma that they are usually intrinsically hydrophobic which lacks of emission in water and modification with ionic groups to access water solubility may result in poor membrane permeability.Fluorescent dyes with strong fluorescence emission in both nonpolar and polar solvents are highly desirable.In this manuscript,we reported a strategy to develop fluorescent BODIPY dyes via installation of amide moiety at meso position of 1,3,5,7-tetramethyl-BODIPY and discovered that N,N'-dialkylsubstituted BODIPY amides possessed highly fluorescent emission with favorable environment-insensitive properties.

关键词： Fluorescent dyes BODIPY Environment-insensitive Bioimaging

Na^+-permeable channels of human sperm membrane reassembled into giant liposome

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Chinese Science Bulletin 2001年第13期46卷 1085-1088页

作者： Zhang Guangping bai junping Shi Yuliang Chinese Acad Sci Shanghai Inst Biol Sci Shanghai Inst Physiol Shanghai 200031 Peoples R China

Previous data showed that a Na+-transmem-brane flux was accompanied with acrosome reaction of sperm. However, the electrophysiological recording and characterization of Na+ current in human sperm membrane have not been yet reported. In the present investigation, membrane proteins extracted from human sperms were reassembled into liposome bilayer, and then the liposomes were fused by dehydration-rehydration into giant liposomes with the diameter of more than 10μm. By patch clamping the giant liposomes two kinds of single channel currents were recorded in a NaCl solution system. Both of them were Na+-carried, TTX-sensitive and strongly rectifying, but with different unit conductance and open probability. Moreover, bursting activity and channel-substates as well as two open time constants were observed in the larger channel.

关键词： patch clamp giant liposome single channel human sperm Na+-channel.

地方高校图书馆专业分馆建设存在的问题、原因和解决方案——以内蒙古某高校F分馆为例

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内蒙古科技与经济 2022年第8期 126-127,130页

作者：白俊萍内蒙古师范大学内蒙古呼和浩特010018

以内蒙古某高校F分馆为例,分析了我国地方高校图书馆专业分馆建设存在有体制机制不顺、软硬件条件不足、服务水平低等问题,提出了专业分馆建设需要明确定位、理顺机制,加大投入水平等解决方案。

关键词：地方高校图书馆专业分馆现状问题

Genetic and pathogenic characterization of new infectious bronchitis virus strains in the GVI-1 and GI-19 lineages isolated in central China

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Journal of Integrative Agriculture 2024年第7期23卷 2407-2420页

作者： Yuhan Yang Dou Wang Yaning bai Wenyan Huang Shimin Gao Xingchen Wu Ying Wang Jianle Ren Jinxin He Lin Jin Mingming Hu Zhiwei Wang Zhongbing Wang Haili Ma junping Li Libin Liang College of Veterinary Medicine Shanxi Agricultural UniversityJinzhong 030801China Shanxi Provincial Animal Disease Prevention and Control Center Taiyuan 030000China

Avian infectious bronchitis(IB)is a highly contagious infectious disease caused by infectious bronchitis virus(IBV),which is prevalent in many countries worldwide and causes serious harm to the poultry industry.At present,many commercial IBV vaccines have been used for the prevention and control of IB;however,IB outbreaks occur frequently.In this study,two new strains of IBV,SX/2106 and SX/2204,were isolated from two flocks which were immunized with IBV H120 vaccine in central China.Phylogenetic and recombination analysis indicated that SX/2106,which was clustered into the GI-19 lineage,may be derived from recombination events of the GI-19 and GI-7 strains and the LDT3-A vaccine.Genetic analysis showed that SX/2204 belongs to the GVI-1 lineage,which may have originated from the recombination of the GI-13 and GVI-1 strains and the H120 vaccine.The virus cross-neutralization test showed that the antigenicity of SX/2106 and SX/2204 was different from H120.Animal experiments found that both SX/2106 and SX/2204 could replicate effectively in the lungs and kidneys of chickens and cause disease and death,and H120 immunization could not provide effective protection against the two IBV isolates.It is noteworthy that the pathogenicity of SX/2204 has significantly increased compared to the GVI-1 strains isolated previously,with a mortality rate up to 60%.Considering the continuous mutation and recombination of the IBV genome to produce new variant strains,it is important to continuously monitor epidemic strains and develop new vaccines for the prevention and control of IBV epidemics.

关键词： infectious bronchitis virus GI-19 lineage GVI-1 lineage complete genome recombination pathogenicity

Genetic and biological properties of H9N2 avian influenza viruses isolated in central China from 2020 to 2022

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Journal of Integrative Agriculture 2024年第8期 2778-2791页

作者： Libin Liang Yaning bai Wenyan Huang Pengfei Ren Xing Li Dou Wang Yuhan Yang Zhen Gao Jiao Tang Xingchen Wu Shimin Gao Yanna Guo Mingming Hu Zhiwei Wang Zhongbing Wang Haili Ma junping Li College of Veterinary Medicine Shanxi Agricultural University Shanxi Provincial Animal Disease Prevention and Control Center

TheH9N2subtypeofavianinfluenzavirus(AIV)iswidelyprevalentinpoultryandwildbirdsglobally,andhas become the predominant subtype circulating in poultry in China.The H9N2 AIV can directly or indirectly (by serving asa“donorvirus”)infecthumans,posingasignificantthreattopublichealth.Currently,thereisalackofindepth research on the prevalence of H9N2 viruses in Shanxi Province, central China.In this study, we isolated 14H9N2 AIVs from October 2020 to April 2022 in Shanxi Province, and genetic analysis revealed that these viruses belonged to 7 different genotypes.Our study on animals revealed that the H9N2 strains we identified displayed hightransmissionefficiencyamongchickenpopulations,andexhibiteddiversereplicationabilitieswithinthese birds.These viruses could replicate efficiently in the lungs of mice, with one strain also demonstrating the capacity to reproduce in organs like the brain and kidneys.At the cellular level, the replication ability of different H9N2 strains was evaluated using plaque formation assays and multi-step growth curve assays, revealing significant differences inthereplicationandproliferationefficiencyofthevariousH9N2virusesatthecellularlevel.Theantigenicity analysis suggested that these isolates could be classified into 2 separate antigenic clusters.Our research provides crucial data to help understand the prevalence and biological characteristics of H9N2 AIVs in central China.It also highlights the necessity of enhancing the surveillance of H9N2 AIVs.

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霍西煤田襄汾矿区南部煤层贫气成因分析

中国煤炭地质 2016年第7期28卷 20-25页

作者：白俊萍山西省煤炭地质勘查研究院太原030031

襄汾矿区南部钻孔煤样瓦斯解吸及测试数据显示,研究区煤层甲烷平均含量均小于2 m L/g·daf,甲烷浓度平均仅为20.1%,属较贫甲烷型—放贫甲烷型煤储层,瓦斯风化带最大深度达1 222.5 m。对主采煤层有机质组分及有机质成熟度研究表明,本区... 详细信息

襄汾矿区南部钻孔煤样瓦斯解吸及测试数据显示,研究区煤层甲烷平均含量均小于2 m L/g·daf,甲烷浓度平均仅为20.1%,属较贫甲烷型—放贫甲烷型煤储层,瓦斯风化带最大深度达1 222.5 m。对主采煤层有机质组分及有机质成熟度研究表明,本区煤层物性条件较好,属中等生气型储层,并已经历成气作用的热降解气阶段,局部可达热裂解气阶段。研究认为:生烃期后构造抬升及张性正断层发育密集导致煤层气大量解吸、逸散是本区煤层贫气的成因。

关键词：襄汾矿区瓦斯风化带有机质组分有机质成熟度构造抬升正断层

基于Power World仿真的小水电并网的研究

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科技通报 2014年第7期30卷 158-161页

作者：白俊平程浩忠柳睿河北机电职业技术学院电气工程系河北邢台054000 电力传输与功率变换控制教育部重点实验室上海交通大学电气工程系上海200240

小水电的接入对配电网的电压分布有直接的影响,影响的大小和小水电的接入位置有很大的关系。本文采用美国伊利诺伊大学开发的电力系统仿真软件包Power World对福建某地区一个47节点,3电源网络进行仿真分析,仿真结果直观、形象的反映出... 详细信息

小水电的接入对配电网的电压分布有直接的影响,影响的大小和小水电的接入位置有很大的关系。本文采用美国伊利诺伊大学开发的电力系统仿真软件包Power World对福建某地区一个47节点,3电源网络进行仿真分析,仿真结果直观、形象的反映出电网电压的分布情况,研究了小水电在丰水期、枯水期接入电网位置对电网电压参数的影响,并给出了结论,小水电并网后适合接在电网中间偏末端。

关键词：小水电并网枯水期丰水期仿真分析

糖尿病视网膜病变玻璃体切除术后再出血吸收状况分析

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医学理论与实践 2016年第23期29卷 3187-3188,3194页

作者：白俊平山西阳泉煤业(集团)有限责任公司第三医院眼科山西省阳泉市045008

目的:比较糖尿病视网膜病变玻璃体切除术后再出血与糖尿病视网膜病变首次玻璃体出血吸收状况的差异。方法:选择在我院眼科就诊的糖尿病视网膜病变曾行玻璃体切除术后再出血患者15例作为研究组,首次玻璃体出血患者15例作为对照组。对两... 详细信息

目的:比较糖尿病视网膜病变玻璃体切除术后再出血与糖尿病视网膜病变首次玻璃体出血吸收状况的差异。方法:选择在我院眼科就诊的糖尿病视网膜病变曾行玻璃体切除术后再出血患者15例作为研究组,首次玻璃体出血患者15例作为对照组。对两组病例的出血吸收状况和出血吸收时间进行比较。采用Fisher确切概率法检验,P<0.05为差异有统计学意义。结果:玻璃体切除术后再出血患者15只眼,出血明显吸收[10只眼,(20±2)d],吸收[3只眼,(15±2)d],略吸收[2只眼,(10±1)d];而首次玻璃体出血患者15只眼,出血明显吸收[2只眼,(62±5)d],吸收[5只眼,(30±3)d],略吸收[8只眼,(15±2)d],两组比较差异有统计学意义。结论:玻璃体切除术后再出血吸收状况有明显好转。

关键词：糖尿病视网膜病变玻璃体切除术后再出血首次玻璃体出血吸收情况出血原因

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高层住宅小区地下车库自动喷淋系统设计浅谈

科技传播 2011年第5期3卷 75-75,73页

作者：白君平上海新建设建筑设计有限公司上海200092

随着经济的发展,出现了大量的高层住宅小区,小区基本上包括高层住宅、多层住宅和地下车库组成。此种小区通常情况下只有地下车库需要设置自动喷淋系统,而消防水箱的水箱间一般设置在建筑群中最高建筑物的屋顶处。本文结合中国金色兰亭... 详细信息

随着经济的发展,出现了大量的高层住宅小区,小区基本上包括高层住宅、多层住宅和地下车库组成。此种小区通常情况下只有地下车库需要设置自动喷淋系统,而消防水箱的水箱间一般设置在建筑群中最高建筑物的屋顶处。本文结合中国金色兰亭住宅小区地下车库自动喷淋系统的实际情况,对高层住宅小区自动喷淋系统进行了论述。

关键词：高层住宅小区自动喷淋消防水箱消防水池喷淋泵

人分泌型磷脂酶A2 GIIE的E54突变及其对底物选择性的影响

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生物工程学报 2021年第7期37卷 2513-2521页

作者：侯淑琳白俊平陆昕张玉龙许婷婷解军山西医科大学基础医学院出生缺陷与细胞再生山西省重点实验室山西太原030001 中国科学院广州生物医药与健康研究院呼吸疾病国家重点实验室广东广州510530

人分泌型磷脂酶A2 GIIE (Human secreted phospholipase A2 GIIE,hGIIE)通过发挥酶催化作用,参与炎症反应和脂代谢过程。为了揭示hGIIE的底物选择机制,文中对hGIIE进行了定点突变,采用毕赤酵母Pichia pastoris重组表达突变体蛋白,然后... 详细信息

人分泌型磷脂酶A2 GIIE (Human secreted phospholipase A2 GIIE,hGIIE)通过发挥酶催化作用,参与炎症反应和脂代谢过程。为了揭示hGIIE的底物选择机制,文中对hGIIE进行了定点突变,采用毕赤酵母Pichia pastoris重组表达突变体蛋白,然后通过阳离子交换和分子排阻两步法纯化蛋白,最后用等温微量热滴定仪测定酶活性。hGIIE的结构分析显示,氨基酸E54可能与GIIE的底物头基选择性有关,经过同源序列比对,拟将E54突变为丙氨酸(A)、苯丙氨酸(F)和赖氨酸(K)。突变体E54A、E54F和E54K在毕赤酵母组成型表达系统中实现重组表达,通过两步纯化,纯度达到90%以上。酶活性实验显示,突变体与底物1,2-二己酰卵磷脂(1,2-dihexanoyl-snglycero-3-phosphocholine,DHPC)和1,2-二己酰磷酸甘油(1,2-dihexyl phosphate glycerol,DHPG)的亲和力发生改变,其中突变体E54K与DHPG的Km值为突变前的0.39倍,亲和力明显增强;突变体E54F与DHPC的Km值为突变前的1.93倍,亲和力明显减弱。hGIIE的E54突变体蛋白与磷脂底物的亲和力发生明显改变,说明E54在hGIIE的磷脂底物选择性水解过程中起重要作用。

关键词：分泌型磷脂酶A2 GIIE 异源表达突变底物选择性