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Journal of Innovative Optical Health Sciences 2018年第1期11卷 13-21页

作者： Svitlana MLevchenko Artem Pliss junle qu Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province College of Optoelectronic Engineering Shenzhen University ShenzhenGuangdong Province 518060P.R.China Institute for Lasers Photonics and Biophotonics University at BuffaloState University of New York BuffaloNY 14260-3000USA

Fluorescence litime imaging(FLIM)is an effective noninvasive bioanalytical tol based onmeasuring fuorescent lifetime of fuorophores.A growing number of FLIM studies utilizes ge-netically engineered fluorescent proteins targeted to specific subcellular structures to probe localmolecular environment,which opens new directions in cell *** paper highlights theunconventional applications of FLIM for studies of molecular processes in diverse organelles oflive cultured cells.

关键词： Fluorescence lifetime imaging fluorescent proteins bioimaging intracellular procescs

Introduction to the Special Issue on Fluorescence Lifetime Imaging Microscopy(FLIM)

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Journal of Innovative Optical Health Sciences 2019年第5期12卷 1-2页

作者： junle qu Liwei Liu Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen UniversityShenzhen 518060P.R.China

Fluorescence Lifetime Imaging Microscopy(FLIM)is an advanced tool that enables the description of exponential decay rate distribution of fuorescent molecules in the *** technique has been broadly used in biomedicine,material science,chemistry,and other related research fields,due to its ability to illustrate both localization of specific fuorophores and fuorophores'local microenvironment,and it is superior to fluorescence intensity based imaging.

关键词： Fluorescence exponential enable

Controllable emission bands and morphologies of high-quality CsPbX3 perovskite nanocrystals prepared in octane

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Nano Research 2018年第9期11卷 4654-4663页

作者： Shuai Ye Mengjie Zhao Jun Song junle qu Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Optoelectronic Engineering Shenzhen University Shenzhen 518060 China

Halide perovskite （CsPbX3, X = C1, Br, or I） quantum dots have received increasing attention as novel colloidal nanocrystals （NCs）. Accurate control of emission bands and NC morphologies are vital prerequisites for most CsPbX3 NC practical applications. Therefore, a facile method of synthesizing CsPbX3 （X = C1, Br, or I） NCs in the nonpolar solvent octane was developed. The process was conducted in air at - 90℃ to synthesize high-quality CsPbX3 NCs showing 12-44 nm wide emission and high photoluminescence quantum yield, exceeding 90%. An in situ anion-exchange method was developed to tune CsPbX3 NC photoluminescence emission, using PbX2 dissolved in octane as the halide source. NC morphology was controlled by dissolving specific metal-organic salts in the precursor solution prior to nucleation, and nanocubes, nanodots, nanosheets, nanoplatelets, nanorods, and nanowires were obtained following the same general method providing a facile, versatile route to controlling CsPbX3 NC emission bands and morphologies, which will broaden the range of CsPbX3 NC practical applications.

关键词： CsPbX3 quantum dots nonpolar solvent octane controllable composition controllable morphology high quantum yield

Antibacterial activity of Cu_2O and Ag co-modified rice grains-like ZnO nanocomposites

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Journal of Materials Science & Technology 2018年第12期34卷 2359-2367页

作者： Dunhua Hong Guangzhong Cao junle qu Yuanming Deng Jiaonin Tang Shenzhen Key Laboratory of Electromagnetic Control College of Mechatronics and Control EngineeringShenzhen University Key Lab of Optoelectronic Devices and Systems of Ministry of Education/Guangdong Province College of Optoelectronic EngineeringShenzhen University Shenzhen Key Laboratory of Special Functional Materials College of Materials Science and EngineeringShenzhen University

In this work, a series of Cu2O-Ag/ZnO, Cu2O/ZnO and Ag/ZnO nanocomposites with various compositions were prepared via a hydrothermal method followed by chemical modification, and their antibacterial performance was investigated in detail. X-ray powder diffraction, scanning electron microscopy and transmission electron microscopy results confirmed that 31 nm Cu20 and 30 nm Ag nanoparticles are well-dispersed on 202 nm ZnO grains to form a Cu2O/ZnO and Ag/ZnO heterojunction, respectively. The bi-heterojuction structure in the Cu20-Ag/ZnO provided a synergistic effect on antibacterial activity, and the(Cu2O)0.04Ag0.06ZnO0.9nanocomposites showed the highest antimicrobial activity of all samples with minimum inhibitory concentration and minimum bactericidal concentration against Escherichia coli and Staphylococcus aureus as low to 31.25 μg/mL, 250μg/mL, 125μg/mL and 500μg/mL, respectively. This is the first report of the antibacterial activities of Cu2O and Ag co-modified ZnO nanocomposites.

关键词： Antibacterial activity Bi-heterojuction structure Antibacterial mechanism Hydrothermal route Cu_2O-Ag/ZnO nanocomposite

PEGylated liposomal photosensitizers as theranostic agents for dual-modal photoacoustic andffuorescence imaging-guided photodynamic therapy

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Journal of Innovative Optical Health Sciences 2019年第3期12卷 32-40页

作者： Hao Xu Yubin Liu junle qu Zhen Yuan College of Optoelectronic Engineering Shenzhen University Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province ShenzhenGuangdongP.R.China Faculty of Health Sciences University of Macao TaipaMacao SAR 999078P.R.China

The photosensitizer(PS)as photodynamic therapy(PDT)agent,can also serve as the contrast agent for dual-modalffuorescence imaging(FLI)and photoacoustic imaging(PAI)for precise cancer *** this study,the PAI capability of commercial PS,benzoporphyrin derivative monoacid ring-A(BPD)were examined and compared with that from the other PSs and dyes such as TPPS_(4),Cy5 dye and *** discovered that BPD exhibited its advantage as contrast agent for ***,BPD can also serve as the contrast agent for enhanced *** particular,the PEGylated nanoliposome(PNL)encapsulated BPD(LBPD)was produced for contrast enhanced dual-modal FLI and PAI and imaging-guided high-efficiency *** FLI and PAI results demonstrated the significant accumulation of LBPD both within and among individual tumor during 24 h monitoring for in vivo experiment ***-vitro and in-vivo PDT tests were also performed,which showed that LBPD have higher PDT efficiency and can easily break the blood vessel of tumor tissues as compared to that from *** was discovered that LBPD has great potentials as a diagnosis and treatment agent for dual-modal FLI and PAI-guided PDT of cancer.

关键词： Liposome photosensitizer photodynamic therapy photoacoustic imaging ffuorescence imaging

Development of a hydrogen peroxide-responsive and oxygen-carrying nanoemulsion for photodynamic therapy against hypoxic tumors using phase inversion composition method

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Journal of Innovative Optical Health Sciences 2021年第2期4卷 75-83页

作者： Liang Hong Jia Zhang Junxian Geng junle qu Liwei Liu Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Optoelectronic Engineering Shenzhen University Shenzhen 518060P.R.China

Photodynamic therapy(PDT)has become an attractive tumor treatment modality because of its noninvasive feature and low side ***,extreme hypoxia inside solid tumors severely impedes PDT therapeutic *** overcome this obstacle,various strategies have been developed *** them,in situ oxygen generation,which relies on the decom-position of tumor endogenous H_(2)O_(2),and oxygen delivery tactic using high oxygen loading capacity of hemoglobin or perfluorocarbons,have been widely *** in situ oxygen generation strategy has high specificity to tumors,but its oxygen-generating efficiency is lim-ited by the intrinsically low tumor H_(2)O_(2)*** contrast,the oxygen delivery approach holds advantage of high oxygen loading efficiency,nevertheless lacks tumor *** this work,we prepared a nanoemulsion system containing H_(2)O_(2)-responsive catalase,highly efficient ox-ygen carrier perfiuoropolyether(PFPE),and a near-infrared(NIR)light activatable photo-sensitizer IR780,to combine the high tumor specificity of the in situ oxygen generation strategy and the high efficiency of the oxygen delivery *** concisely prepared nanoplatform exhibited enhanced and H_(2)O_(2)-controllable production of singlet oxygen under light excitation,satisfactory cytocompatibility,and ability to kill cancer cells under NIR light *** highlights the potential of this novel nanoplatform for highly efficient and selective NIR light mediated PDT against hypoxic *** research provides new insight into the design of intelligent nanoplatform for relieving tumor hypoxia and enhancing the oxygen-dependent PDT effects in hypoxic tumors.

关键词： Hypoxia catalase oxygen delivery perfluorocarbon near-infrared

Green emitted CdSe@ZnS quantum dots for FLIM and STED imaging applications

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Journal of Innovative Optical Health Sciences 2019年第5期12卷 79-85页

作者： Mengjie Zhao Shuai Ye Xiao Peng Jun Song junle qu Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University Shenzhen 518060P.R.China

Inorganic quantum dots(QDs)have excellent optical properties,such as high°uorescence intensity,excellent photostability and tunable emission wavelength,etc.,facilitating them to be used as labels and probes for *** this study,CdSe@ZnS QDs are used as probes for Fluorescence lifetime imaging microscope(FLIM)and stimulated emission depletion(STED)nanoscopy *** emission peak of CdSe@ZnS QDs centered at 526 nm with a narrow width of 19 nm and the photoluminescence quantum yield(PLQY)was 64%.The QDs presented excellent anti-photobleaching property which can be irradiated for 400 min by STED laser with 39.8 *** lateral resolution of 42.0 nm is demonstrated for single QDs under STED laser(27.5 mW)***,the CdSe@ZnS QDs were for the first time used to successfully label the lysosomes of living HeLa cells and 81.5 nm lateral resolution is obtained indicating the available super-resolution applications in living cells for inorganic QD ***,Eca-109 cells labeled with the CdSe@ZnS QDs was observed with FLIM,and their fluorescence lifetime was around 3.1 ns,consistent with the in vitro value,suggesting that the QDs could act as a satisfactory probe in further FLIM-STED experiments.

关键词： CdSe@ZnS QDs FLIM STED living cells

Shedding light on biology and healthcare--preface to the special issue on Biomedical Optics

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Light(Science & Applications) 2022年第7期11卷 1315-1317页

作者： Peng Xi Xunbin Wei junle qu Valery V.Tuchin Department of Biomedical Engineering College of Future TechnologyPeking University100871BeijingChina Department of Biomedical Engineering Peking University100081BeijingChina Center for Biomedical Optics and Photonics(CBOP)&College of Physics and Optoelectronic Engineering Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of EducationShenzhen University518060ShenzhenChina Saratov State University 83 Astrakhanskaya str.Saratov410012Russia

During the past decade,there has been an explosion in the development of novel optical techniques in biomedical research and clinical *** progress has been made in optical imaging and detection to provide necessary biological information for disease diagnosis and in-depth understanding of biological ***,recent advances integrating laser technology with biomarkers have enabled novel diagnostic and treatment approaches for different types of diseases.

关键词： optical integrating diagnosis

Scanless multitarget-matching multiphoton excitation fluorescence microscopy

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Journal of Innovative Optical Health Sciences 2018年第2期11卷 1-7页

作者： Junpeng Qiu Lei Wang Bruce Zhi Gao junle qu Yonghong Shao College of Optoelectronics Engineering Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Shenzhen University Shenzhen 518060P.R.China Department of Bioengineering and COMSET Clemson University ClemsonSC 29634USA

Using the combination of a refective blazed grating and a reflective phase-only difractive spatiallight modulator(SLM),scanless multitarget-matching multiphoton excitation fuorescence ***(SMTM-MP M)was *** SLM shaped an incoming mode-locked,near-infraredTi:sapphire laser beam into an excitation pattern with addressable shapes and sizes that matchedthe samples of interest in the field of *** and spatial focusing were simultaneouslyrealized by combining an objective lens and a blazed *** fluorescence signal fromilluminated areas was recorded by a two-dimensional sCMOS *** with a conventional temporal focusing multiphoton microscope,our microscope achieved effective use of thelaser power and decreased photodamage with higher axial resolution.

关键词： Multitarget-matching multiphoton microscopy SLM temporal focusing

Fluorescence lifetime imaging for studying DNA compaction and gene activities

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Light(Science & Applications) 2021年第12期10卷 2349-2359页

作者： Svitlana M.Levchenko Artem Pliss Xiao Peng Paras N.Prasad junle qu Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhenGuangdong 518060China Department of Cell Biophysics Faculty of BiochemistryBiophysics and BiotechnologyJagiellonian University30-387 KrakowPoland Institute for Lasers Photonics and BiophotonicsUniversity at BuffaloState University of New YorkBuffaloNY 14260-3000USA

Optical imaging is a most useful and widespread technique for the investigation of the structure and function of the cellular ***,an analysis of immensely convoluted and irregularly compacted DNA polymer is highly challenging even by modern super-resolution microscopy *** we propose fluorescence lifetime imaging(FLIM)for the advancement of studies of genomic structure including DNA compaction,replication as well as monitoring of gene *** proposed FLIM assay employs two independent mechanisms for DNA compaction *** mechanism relies on the inverse quadratic relation between the fluorescence lifetimes of fluorescence probes incorporated into DNA and their local refractive index,variable due to DNA compaction *** mechanism is based on the Förster resonance energy transfer(FRET)process between the donor and the acceptor fluorophores,both incorporated into *** these proposed mechanisms were validated in cultured *** obtained data unravel a significant difference in compaction of the gene-rich and gene-poor pools of genomic *** show that the gene-rich DNA is loosely compacted compared to the dense DNA domains devoid of active genes.

关键词： expression. lifetime donor