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中国畜牧兽医学会动物繁殖学分会第十五届学术研讨会

作者： Kazuhiro Kikuchi Michiko Nakai junko noguchi Junya Ito Naomi Kashiwazaki Hiroyuki Kaneko Division of Animal Sciences National Institute of Agrobiological SciencesTsukubaIbaraki Laboratory of Animal Reproduction School of Veterinary MedicineAzabu UniversitySagamiharaKanagawa

In vitro fertilization of in vitro matured oocytes in pigs has become the most popular method of studying gametogenesis and embryogenesis in this *** addition,because of recent advances in in vitro culture of those embryos,in vitro embryo production now enables us to generate viable embryos as successfully as for in vivo-derived embryos and with less cost and in less *** technologies contribute not only to developments in reproductive physiology and agriculture but also to conservation of porcine genetic resources. Until now,conservation of pig genetic recourses has been limited by cryopreservation of sperm and,in some cases, in vivo-derived *** aspects may decrease the general activates in ex situ conservation(gene banking) in this *** recent years,however,unique technologies such as xenografting of gonadal(testicular and ovarian) tissue into immunodeficient experimental animals,which are combined with in vitro embryo production technologies,have been developed to help conservation and utilization of gamete *** have already shown fertilizing ability and developmental ability of porcine embryos from gametes grown in ***,we discuss the possibility of conservation and utilization of pig resources by xenografting to mice.

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Efficacy of an Abduction Brace in Preventing Initial Dislocation in the Early Postoperative Period after Primary Total Hip Arthroplasty

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Surgical Science 2012年第3期3卷 131-135页

作者： Yoshinori Ishii Hideo noguchi Mitsuhiro Takeda junko Sato Yoichiro Domae Department of Orthopaedic Surgery Shibata Prefectural Hospital Niigata Japan Ishii Orthopaedic and Rehabilitation Clinic Saitama Japan

We hypothesized that a hip brace may prevent the initial dislocation in the early postoperative period after total hip arthroplasty (THA). We performed a prospective evaluation of the efficacy of a brace in preventing dislocation in 54 primary THAs in 43 patients. All of the patients wore braces for 3 weeks postoperatively. The occurrence of dislocation was evaluated six months postoperatively. There were no cases of dislocation while wearing a brace, while two posterior dislocations occurred 8 and 12 days postoperatively while picking something up from the floor without a brace in so-called provocative positions. The results of this study suggest that a hip brace helps patients to recognize careless provocative positions and prevents the initial hip dislocation in this period instead of usual postoperative management for an average of 6 weeks after discharge, such as a high toilet seat, restricted hip flexion in the activities of daily living, use of a reacher or grabber, an abduction pillow, and a high chair.

关键词： Hip Brace Dislocation Total Hip Arthroplasty

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Evaluation of Fever and Its Related Factors during 4 Weeks after Primary Total Hip Arthroplasty

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Surgical Science 2013年第1期4卷 1-6页

作者： Yoshinori Ishii Hideo noguchi Mitsuhiro Takeda junko Sato Takeshi Yamamoto Yo-Ichiro Domae Department of Orthopaedic Surgery Shibata Prefectural Hospital Shibata Japan Ishii Orthopaedic and Rehabilitation Clinic Gyoda Japan

The purpose of this study was to describe the incidence and characteristics of postoperative fever (POF;≥38℃) and clarify the correlation between POF and febrile-related factors during 4 weeks after primary total hip arthroplasty (THA). A total of 56 patients were included in the study (67 THAs). The preoperative diagnosis for all patients was osteoarthritis. The average follow-up period was 90 months. No patients developed deep infection during the follow-up period. The maximum mean peak daily temperature was 37.7℃ on the day of the surgery and the next day. Fifty-two percent of patients experienced POF, with most developing a maximum temperature (MT) on the day of surgery and the latest occurring by postoperative day (POD) 3. Only C-reactive protein (R = 0.384, p = 0.001) among the febrile-related factors had a weak correlation with MT. Four hips showed a POF of >38℃ later than POD 7;all four of these patients were diagnosed with a urinary tract infection. POF is common and may be part of a normal inflammatory response to tissue injury after THA. More than half of patients may develop POF within 1 week, with most developing MT on POD 0 and the latest occurring by POD 3. In addition, urinary tract infections might be an initial reason for POF in the subsequent 3 weeks.

关键词： Total Hip Arthroplasty Post-Operative Fever Maximum Temperature C-Reactive Protein Urinary Tract Infection

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Glycosylation and secretion of human α-amylases

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Advances in Biological Chemistry 2012年第1期2卷 10-19页

作者： Shou Takashima junko Amano Laboratory of Glycobiology The Noguchi Institute Tokyo Japan New Industry Creation Hatchery Center Tohoku University Sendai Japan

Three human α-amylases exist: Amy1 (salivary amylase), Amy2A (pancreatic amylase), and Amy2B (expressed in various tissues). These amylases share a 97% - 99% amino acid sequence identity, and two potential N-glycosylation sites (N427 and N476) are commonly found in the C-terminal region. In general, salivary amylase is more frequently glycosylated than pancreatic amylase, and it is still uncertain why differences in the glycosylation pattern among human amylase iso-zymes occur. In this study, we found that there was no significant change of ratio of glycosylated molecules among isozymes produced by the same cultured cells, indicating that glycosylation of amylase is influenced by the type of cell producing the enzyme rather than being an inherent property of the amylase isozymes. We analyzed the glycosylation efficiency of N-glycosylation sites in recombinant Amy2A mutants produced by HEK293 cells and found that glycosylation efficiencies of N427 and N476 were 3% - 18% and 40% - 52%, respectively, indicating that the major N-glycosylation site of glycosylated Amy2A produced by HEK293 cells is N476. The difference in the glycosylation efficiency of each N-glycosylation site also seemed to contribute in part to generate different glycosylation patterns of human amylases. We also confirmed that the C-terminal region of human amylase plays a critical role in secretion, although glycosylation does not play a part in this effect.

关键词： Amylase Gycosylation Efficiency N-Glycan Secretion

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