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Hierarchically Inverse Opal Porous Scaffolds from Droplet Microfluidics for Biomimetic 3D Cell Co-Culture

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Engineering 2021年第12期7卷 1778-1785页

作者： Changmin Shao Yuxiao Liu junjie chi Fangfu Ye Yuanjin Zhao Department of Rheumatology and Immunology Institute of Translational MedicineAffiliated Drum Tower HospitalMedical School of Nanjing UniversityNanjing 210008China Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)Wenzhou 325038China Wenzhou Institute University of Chinese Academy of SciencesWenzhou 325016China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China

With the advantages of better mimicking the specificity of natural tissues,three-dimensional(3D)cell culture plays a major role in drug development,toxicity testing,and tissue ***,existing scaffolds or microcarriers for 3D cell culture are often limited in size and show suboptimal performance in simulating the vascular complexes of living ***,we present a novel hierarchically inverse opal porous scaffold made via a simple microfluidic approach for promoting 3D cell co-culture *** designed scaffold is constructed using a combined concept involving an emulsion droplet template and inert polymer *** work demonstrates that the resultant scaffolds ensure a sufficient supply of nutrients during cell culture,so as to achieve large-volume cell *** addition,by serially planting different cells in the scaffold,a 3D co-culture system of endothelial-cellencapsulated hepatocytes can be developed for constructing certain functional *** is also demonstrated that the use of the proposed scaffold for a co-culture system helps hepatocytes to maintain specific in vivo *** hierarchically inverse opal scaffolds lay the foundation for 3D cell culture and even the construction of biomimetic tissues.

关键词： Microfluidics Inverse opal Cell culture Droplet Biomaterial

Responsive Inverse Opal Scaffolds with Biomimetic Enrichment Capability for Cell Culture

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Research 2019年第1期2019卷 423-432页

作者： Changmin Shao Yuxiao Liu junjie chi Jie Wang Ze Zhao Yuanjin Zhao State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China

Three-dimensional(3D)porous scaffolds have a demonstrated value for tissue engineering and regenerative *** by the predation processes of marine predators in nature,we present new photocontrolled shrinkable inverse opal graphene oxide(GO)hydrogel scaffolds for cell enrichment and 3D *** scaffolds with adjustable pore sizes and morphologies were created using a GO and N-isopropylacrylamide dispersed solution as a continuous phase of microfluidic emulsions for polymerizing and *** of the interconnected porous structures and the remotely controllable volume responsiveness of the scaffolds,the suspended cells could be enriched into the inner spaces of the scaffolds through predator-like swallowing and discharging *** cells concentrated in the scaffold pores could form denser 3D spheroids more quickly via the controlled compression force caused by the shrinking of the dynamic *** importantly,with a program of scaffold enrichment with different cells,an unprecedented 3D multilayer coculture system of endothelial-cellencapsulated hepatocytes and fibroblasts could be generated for applications such as liver-on-a-chip and bioartificial *** was demonstrated that the resultant multicellular system offered significant improvements in hepatic functions,such as albumin secretion,urea synthesis,and cytochrome P450 *** features of our scaffolds make them highly promising for the biomimetic construction of various physiological and pathophysiological 3D tissue models,which could be used for understanding tissue level biology and in vitro drug testing applications.

关键词： sizes dispersed suspended

Supramolecular G-quadruplex hydrogels:Bridging fabrication to biomedical application

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Journal of Materials Science & Technology 2023年第24期155卷 238-252页

作者： Yuanfeng Li junjie chi Pingwei Xu Xing Dong Anh-Tuan Le Keqing Shi Yong Liu Jian Xiao Translational Medicine Laboratory The Center of Wound Healing and Regenerative Medicine the First Affiliated Hospital of Wenzhou Medical University Zhejiang 325035 China School of Pharmaceutical Sciences Key Laboratory of Biotechnology and Pharmaceutical Engineering The Center of Wound Healing and Regenerative Medicine the First Affiliated Hospital Wenzhou Medical University Zhejiang 325035 China Wenzhou Institute University of Chinese Academy of Sciences Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine Vision and Brain Health) Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province Zhejiang 325001 China Phenikaa University Nano Institute (PHENA) Phenikaa University Hanoi 12116 Viet Nam

G-quadruplex hydrogel is a class of self-assembled supramolecular hydrogel formed by guanine *** a biomimetic hydrogel,G-quadruplex hydrogels demonstrate wide biomedical applications,such as drug delivery,tissue engineering,and *** advantages of using G-quadruplex hydrogels include adequate biocompatibility and biodegradability,tunable multifunctionality,and cost-effective and large-scalable fabrication *** this review,we focus on recent progress in the fabrication and characterization of G-quadruplex hydrogels to help readers understand the principles of G-quadruplex hydrogel ***,the applications of G-quadruplex hydrogels in the biomedical area are discussed,aiming to pave the way for downward clinical or industry *** development of G-quadruplex hydrogel is still in its *** hope this review will boost the development of this area and that more applications of G-quadruplex hydrogel will be developed.

关键词： Guanosine DNA hydrogel Self-assembly Drug delivery Tissue engineering

Living Materials for Life Healthcare

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Accounts of Materials Research 2021年第1期2卷 59-70页

作者： Luoran Shang Changmin Shao junjie chi Yuanjin Zhao Zhongshan-Xuhui Hospital The Shanghai Key Laboratory of Medical EpigeneticsInstitutes of Biomedical SciencesFudan UniversityShanghai 200032China Department of Rheumatology and Immunology Institute of Translational MedicineThe Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China Wenzhou Institute University of Chinese Academy of SciencesWenzhouZhejiang 325001China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China

CONSPECTUS:Living material is a type of composite material with living elements integrated into nonliving *** combines the advantages of both living cells and nonliving substances and takes advantage of advanced fabrication *** materials is an emerging new paradigm at the frontiers of materials *** addresses an intriguing question of how to let materials show life-like capabilities and function in an integrative and programmable *** all of the capability and application aspects of living materials,the one related to human health is the most intriguing since it implies that that materials could be made by life and used for ***,living materials exploit the merits of cells in that they can respond to environmental variables,synthesize functional molecules,and span multiple length *** the same time,the abundant coordination between live and nonliving elements opens infinite possibilities for diverse structural and functional *** resultant biohybrid materials are thus endowed with novel functions that recapitulate or even surpass their natural candidates and ultimately shed new light on biodiagnostics and biotherapeutics to address human health *** this Account,we present a concise summary and analysis of living materials and bring it into the context of addressing life healthcare *** start with the fabrication of living materials,including the engineering of living elements and the manufacturing of biohybrid composites through advanced technologies,particularly micropatterning,microfluidics,and threedimensional(3D)*** then shift to the properties and capacities of living materials,such as autonomous motion,sensing and actuation,and highly sensitive *** the basis of these,we show,by representative examples,the healthcare-oriented applications of living materials in both diagnostic and biotherapeutic aspects,including biohybrid sensors and actuators,cell-powered robotics,organ

关键词： composites precise Living

Antibacterial and angiogenic chitosan microneedle array patch for promoting wound healing

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Bioactive Materials 2020年第2期5卷 253-259页

作者： junjie chi Xiaoxuan Zhang Canwen Chen Changmin Shao Yuanjin Zhao Yongan Wang Department of Clinical Laboratory The Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing210008China State Key Laboratory of Toxicology and Medical Countermeasures Institute of Pharmacology and ToxicologyAcademy of Military Medical SciencesBeijing100850China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing210096China

A patch with the capability of avoiding wound infection and promoting tissue remolding is of great value for wound *** this paper,we develop a biomass chitosan microneedle array(CSMNA)patch integrated with smart responsive drug delivery for promoting wound *** possesses many outstanding features such as the natural antibacterial property and has been widely utilized for wound ***,the microstructure of microneedles enables the effective delivery of loaded drugs into the target area and avoids the excessive adhesion between the skin and the ***,vascular endothelial growth factor(VEGF)is encapsulated in the micropores of CSMNA by temperature sensitive ***,the smart release of the drugs can be controllably realized via the temperature rising induced by the inflammation response at the site of *** is demonstrated that the biomass CSMNA patch can promote inflammatory inhibition,collagen deposition,angiogenesis,and tissue regeneration during the wound ***,this versatile CSMNA patch is potentially valuable for wound healing in clinical applications.

关键词： Microneedle chitosan Drug delivery Wound healing Patch

Ellipsoidal porous patch with anisotropic cell inducing ability for inhibiting skin scar formation

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Engineered Regeneration 2022年第3期3卷 262-269页

作者： Wanqing Weng junjie chi Xiaocheng Wang Keqing Shi Fangfu Ye Yuanjin Zhao Joint Center of Translational Medicine The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325035China Department of Clinical Laboratory Nanjing Drum Tower HospitalSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325001China Key Laboratory of Intelligent Treatment and Life Support for Critical Diseases of Zhejiang Provincial Zhejiang Engineering Research Center for Hospital Emergency and Process DigitizationWenzhouZhejiang 325000China Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China

Scar formation has always been a difficult point to overcome in the field of clinical wound ***,we present an ellipsoidal porous patch with cell inducing ability for inhibiting scar *** patch was prepared by stretching a poly(lactic-co-glycolic acid)(PLGA)inverse opal film at the glass transition temperature to form a neatly arranged three-dimensional ellipsoidal porous *** anisotropic structure showed dramatic capability in directing cell growth and arrangement by reconstructing cell ***,the prolifera-tion of cells growing on the stretched patch was significantly suppressed without cell *** addition,benefitting from the abundant and connected nanopores,the patch could be imparted with a potent ability to promote cell migration by encapsulating fibroblast growth factor 2(FGF2)via the second filling of functional gelatin methacryloyl(GelMA)hydrogel into its *** a typical scar model,we have demonstrated that the resultant patch performed well in inhibiting scar formation characterized by inhibiting the excessive proliferation of fibroblasts,decreasing the deposition of type I collagen,reducing the scar index and achieved complete tissue *** results indicate the anisotropic inverse opal patch has an excellent application prospect in inhibiting scar formation during wound repair.

关键词： Inverse opal Biomaterial Porous film Hypertrophic scar Anisotropy

chinese herb microneedle patch for wound healing

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Bioactive Materials 2021年第10期6卷 3507-3514页

作者： junjie chi Lingyu Sun Lijun Cai Lu Fan Changmin Shao Luoran Shang Yuanjin Zhao Translational Medicine Laboratory The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou325035China Department of Burns and Plastic Surgery Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing210008China Zhongshan-Xuhui Hospital The Shanghai Key Laboratory of Medical EpigeneticsInstitutes of Biomedical SciencesFudan UniversityShanghai200032China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing210096China Wenzhou Institute University of Chinese Academy of SciencesWenzhouZhejiang325001China

Traditional chinese medicine and chinese herbs have a demonstrated value for disease therapy and sub-health *** in this area tend to develop new forms to make their applications more convenient and ***,we propose a novel chinese herb microneedle(CHMN)patch by integrating the herbal extracts,Premna microphylla and Centella asiatica,with microstructure of microneedle for wound *** path is composed of sap extracted from the herbal leaves via traditional kneading method and solidified by plant ash derived from the brine induced process of tofu in a well-designed *** the leaves of the Premna microphylla are rich in pectin and various amino acids,the CHMN could be imparted with medicinal efficacy of heat clearing,detoxicating,detumescence and ***,with the excellent pharmaceutical activity of Asiatic acid extracted from Centella asiatica,the CHMN is potential in promoting relevant growth factor genes expression in fibroblasts and showing excellent performance in anti-oxidant,anti-inflammatory and anti-bacterial *** advantages of these pure herbal compositions,we have demonstrated that the derived CHMN was with dramatical achievement in anti-bacteria,inhibiting inflammatory,collagen deposition,angiogenesis and tissue reconstruction during the wound *** results indicate that the integration of traditional chinese herbs with progressive technologies will facilitate the development and promotion of traditional chinese medicine in modern society.

关键词： chinese herb Microneedle Asiatic acid Wound healing Patch

Theoretical screening of cooperative N-bridged dual-atom sites for efficient electrocatalytic nitrogen reduction with remolding insight

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Nano Research 2024年第4期17卷 3413-3422页

作者： Huimei Chen Yan Yang chi Jiao Zhiwen Zhuo junjie Mao Yan Liu Key Laboratory of Functional Molecular Solids Ministry of EducationCollege of Chemistry and Materials ScienceAnhui Normal UniversityWuhu 241002China

The electrocatalytic nitrogen reduction reaction(e-NRR)is a promising alternative method for the Haber–Bosch ***,it still faces many challenges in searching for high activity,stability,and selectivity catalysts and ascertaining the catalytic mechanism with complete ***,a series of graphene-based N-bridged dual-atom catalysts(M1-N-M2/NC)are systematically investigated via first-principle calculation and a high-throughput screening *** result unveils that N_(2) adsorption on M1-N-M2/NC in bridge-on adsorption mode can effectively break the scaling relationship on single-atom catalysts(SACs).Moreover,V-N-Ru/NC and V-N-Os/NC are systematically screened out as promising e-NRR catalysts,with extremely low limiting potentials of-0.20 and-0.18 V,***,the adsorption site competition between*N_(2) and*H,as well as the competitive twin reactions of hydrogen evolution reaction(HER)on intermediates(N_(n)H_(m))during the e-NRR process,is systematically evaluated to form a remodeling insight for the reactions in mechanism,and the e-NRR of new proposed dual-atom catalysts(DACs)is strategically optimized for its high-efficiency performance potential via our remolding insight in e-NRR *** work provides new ideas and insights for the design and mechanism of e-NRR catalysts and an effective strategy for rapidly screening highly efficient e-NRR catalysts.

关键词： nitrogen reduction dual-atom catalysts high-throughput screening hydrogen evolution reaction density functional theory calculations

Recent biomedical applications of bio-sourced materials

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Bio-Design and Manufacturing 2018年第1期1卷 26-44页

作者： Abdelrahman Elbaz Zhenzhu He Bingbing Gao junjie chi Enben Su Dagan Zhang Songqin Liu Hua Xu Hong Liu Zhongze Gu State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University Nanjing 210096 China Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou S uzhou 215123 China Getein Biotech Inc. No. 9 Bofu Road Luhe District Nanjing 211505 Jiangsu China Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device Jiangsu Province Hi-Tech Key Laboratory for Bio-Medical Research School of Chemistry and Chemical Engineering Southeast University Nanjing 211189 China

Natural anisotropic nanostructures occurring in several organisms have gained more and more attention because of their obvious advantages in sensitivity, stability, security, miniaturization, portability, online use, and remote monitoring. Due to the development of research on nature-inspired bionic structures and the demand for highly efficient, low-cost microfabrication techniques, an understanding of and the ability to replicate the mechanism of structural coloration have become increasingly significant. These sophisticated structures have many unique functions and are used in many applications. Many sensors have been proposed based on their novel structures and unique optical properties. Several of these bio-inspired sensors have been used for infrared radiation/thermal, pH, and vapor techniques, among others, and have been discussed in detail, with an intense focus on several biomedical applications. However, many applications have yet to be discovered. In this review, we will describe these nanostructured materials based on their sources in nature and various structures, such as layered, hierarchical, and helical structures. In addition, we discuss the functions endowed by these structures, such as superhydrophobicity, adhesion, and high strength, enabling them to be employed in a number of applications in biomedical fields, including cell cultivation, biosensors, and tissue engineering.

关键词： Nature Bio-sourced Bio-inspired Bio-Materials Biomedical