检索结果-南通市图书馆

Eucommia lignans alleviate the progression of diabetic nephropathy through mediating the AR/Nrf2/HO-1/AMPK axis in vivo and in vitro

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2023年第7期21卷 516-526页

作者： HUANG Qi ZHANG Yinfan JIANG Yueping HUANG Ling liu Qiong OUyang Dongsheng Department of Pharmacy Xiangya HospitalCentral South UniversityChangsha 410008China National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangsha 410008China Hunan Key Laboratory for Bioanalysis of Complex Matrix Samples Changsha Duxact Biotech Co.Ltd.Changsha 411000 China Department of Operating Room Xiangya HospitalCentral South UniversityChangsha 410008China Department of Oncology Xiangya HospitalCentral South UniversityChangsha 410008China Department of Clinical Pharmacology Xiangya HospitalCentral South UniversityChangsha 410008China Institute of Clinical Pharmacology Hunan Key Laboratory of PharmacogeneticsCentral South UniversityChangsha 410028China

Lignans derived from Eucommia ulmoides oliver(Eucommia lignans)inhibit the progression of inflammatory diseases,w.ile their effect on the progression of diabetic nephropathy(DN)remained *** w.rk w.s designed to assess the function of Eucommia lignans in *** major constituents of Eucommia lignans w.re analyzed by UPLC-Q-TOF-MS/*** binding betw.en Eucommia lignans and aldose reductase(AR)w.s predicted by molecular *** lignans(200,100,and 50 mg·kg^(-1))w.re used in model animals to evaluate their renal function *** glomerular mesangial cells(HBZY-1)w.re transfected w.th sh-AR,sh-AMPK,and oe-AR in the presence of high glucose(HG)or HG combined w.th Eucommia lignans to evaluate w.ether Eucommia lignans affected HG-induced cell injury and mitochondrial dysfunction through the AR/Nrf2/HO-1/AMPK *** lignans significantly attenuated the progression of DN in *** lignans notably reversed HG-induced upregulation of inflammatory cytokines and mitochondrial injury,w.ile dow.regulating the levels of Cyto c,caspase 9,AR,and NOX4 in HBZY-1 *** contrast,HG-induced dow.regulation of Nrf2,HO-1 and p-AMPKαlevels w.re abolished by Eucommia ***,knockdow. of AR exerted similar therapeutic effect of Eucommia lignans on DN progression,and AR overexpression reversed the effect of Eucommia *** lignans alleviated renal injury through the AR/Nrf2/HO-1/AMPK ***,these findings might provide evidence for the use of Eucommia lignans in treating DN.

关键词： Diabetic nephropathy(DN) Eucommia lignans Aldose reductase(AR) Nrf2/HO-1/AMPK

牙鲆海水循环养殖系统生物膜上3种细菌的数量与代谢活性

在线全文

学校读者我要写书评

暂无评论

中国水产科学 2009年第1期16卷 97-103页

作者：马悦欣邵华刘长发 SCHNEIDER oliver 晏再生尹丽丽孙丽丽大连水产学院农业部海洋水产增养殖学重点开放实验室 w.geningen IMARES Wageningen URKorringaweg54401 NT YersekeThe Netherlands

从牙鲆(Paralichthysolivaceus)海水循环养殖系统中移动床生物滤器载体生物膜上获取异养细菌、氨化细菌和硝化细菌样本。异养细菌用平板涂布法计数,其代谢活性以培养8h的氧吸收速率表示。氨化细菌、氨氧化细菌(AOB)和亚硝酸氧化细菌(NOB... 详细信息

从牙鲆(Paralichthysolivaceus)海水循环养殖系统中移动床生物滤器载体生物膜上获取异养细菌、氨化细菌和硝化细菌样本。异养细菌用平板涂布法计数,其代谢活性以培养8h的氧吸收速率表示。氨化细菌、氨氧化细菌(AOB)和亚硝酸氧化细菌(NOB)用MPN法计数,其代谢活性分别以培养24hNH4+-N浓度的增加、NH4+-N浓度的减少和NO2--N浓度的减少表示。结果表明,生物膜成熟之后,4种生理类群细菌的数量和代谢活性基本保持稳定。异养细菌数量为107～108CFU-·-m-2(载体),氨化细菌、AOB和NOB数量分别为107～108MPN-·-m-2(载体)、105～106MPN-·-m-2(载体)和105～106MPN-·-m-2(载体)。分批培养测得异养细菌的氧吸收速率、氨化细菌的氨化速率、AOB的氨氧化速率和NOB的亚硝酸氧化速率分别是0.591～0.738g(O2)-·-m-2-·-d-1、0.081～0.135g(NH4+-N)-·-m-2-·-d-1、0.017～0.031g(NH4+-N)-·-m-2-·-d-1和0.020～0.038g(NO2--N)-·-m-2-·-d-1。异养细菌的氧吸收速率、氨化细菌的氨化速率和AOB的氨氧化速率与异养细菌、氨化细菌和AOB的数量呈正相关。移动床生物滤器生物膜的平均氨和亚硝酸盐去除速率为(0.121±0.076)g(NH4+-N)-·-m-2-·-d-1和(0.139±0.181)g(NO2--N)·-m-2-·-d-1。同时测定了底物浓度和pH对氨化细菌、AOB和NOB代谢活性的影响。结果显示,氨化细菌的氨化速率、AOB的氨氧化速率和NOB的亚硝酸氧化速率与底物浓度(蛋白胨、NH4+-N和NO2--N)呈线性相关,pH为8.0时3种生理类群细菌的代谢活性较pH6.5或pH9.5时高。

关键词：移动床生物滤器异养细菌氨化细菌氨氧化细菌亚硝酸氧化细菌

On Higher Order Pyramidal Finite Elements

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Advances in Applied Mathematics and Mechanics 2011年第2期3卷 131-140页

作者： Liping liu Kevin B.Davies Michal Krızek Li Guan Department of Mathematical Sciences Lakehead University955 Oliver RoadThunder BayOntarioP7B 5E1Canada Institute of Mathematics Academy of SciencesZitna 25CZ-11567 Prague 1Czech Republic College of Computer Science Dongguan University of TechnologyDongguan 523808GuangdongChina

In this paper w. first prove a theorem on the nonexistence of pyramidal polynomial basis *** w. present a new.symmetric composite pyramidal finite element w.ich yields a better convergence than the nonsymmetric *** has fourteen degrees of freedom and its basis functions are incomplete piecew.se triquadratic *** space of ansatz functions contains all quadratic functions on each of four subtetrahedra that form a given pyramidal element.

关键词： Pyramidal polynomial basis functions finite element method composite elements three-dimensional mortar elements.

First Record of the Genus w.jayarana (Anura, Ranidae) from Yunnan, China

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Herpetological Research 2023年第1期14卷 116-120,I0006页

作者： Dongyi w. Junkai HUANG Yunhe w. Xiaolong liu Jing CHE Zhiyong YUAN Key Laboratory of Freshw.ter Fish Reproduction and Development Ministry of EducationSouthwest UniversityChongqing 400715China Key Laboratory for Conserving w.ldlife w.th Small Populations in Yunnan Southwest Forestry UniversityKunming 650224YunnanChina Key Laboratory of Rare and Endangered Animal Ecology Guangxi ProvinceCollege of Life ScienceGuangxi Normal UniversityGuilin 541001GuangxiChina State Key Laboratory of Genetic Resources and Evolution Yunnan Key Laboratory of Biodiversity and Ecological Conservation of Gaoligong MountainKunming Institute of ZoologyChinese Academy of SciencesKunming 650223YunnanChina Southeast Asia Biodiversity Research Institute Chinese Academy of SciencesYezinNay Pyi Taw 05282Myanmar

Dear Editor,e family Ranidae is one of the largest and most families of amphibians(Che et al.,2007),w.th 64 recognized genera and429 species(Frost,2022).How.ver,the systematics and natural history of some ranid groups have not been *** such example is the taxonomic relationship betw.en Sylvirana,H ylarana and other closely-related taxa(Che et al.,2007;Pyron and w.ens,2011;oliver et al.,2015).China has a large diversity of ranid frogs,w.th 146 speciesbelonging to 7 genera,and48 species belonging to 7 genera in the southern province of Yunnan(AmphibiaChina,2022).

关键词： Genus Frost genera

生物科学专业本科生设计性实验模式探索——以如何提高杜仲种子萌发率为例

在线全文

学校读者我要写书评

暂无评论

现代农业科技 2016年第20期 287-288页

作者：刘扬陕西学前师范学院陕西西安710061

以如何提高杜仲种子萌发率为例,探讨了生物科学专业设计性实验教学方法,通过对学生分组,要求学生自主查阅资料、设计实验方案、完成实验、得出结论、撰写报告,通过这种方式培养学生的创新能力。

关键词：生物科学专业植物生理学实验设计性实验创新能力

Dietary protocatechuic acid ameliorates inflammation and up-regulates intestinal tight junction proteins by modulating gut microbiota in LPS-challenged piglets

在线全文

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2021年第1期12卷 328-339页

作者： Ruizhi Hu Ziyu He Ming liu Jijun Tan Hongfu Zhang De-Xing Hou Jianhua He Shusong w. Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients College of Animal Science and TechnologyHunan Agricultural UniversityChangsha 410128China Department of Food Science and Biotechnology Faculty of AgricultureKagoshima UniversityKagoshima 890-0065Japan Beijing China-Agri HongKe Bio-Technology Co. Ltd.Beijing 102206China State Key Laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China.

Background: w.aning is one of the major factors that cause stress and intestinal disease in piglets. Protocatechuic acid(PCA) is an active plant phenolic acid w.ich exists in Chinese herb, Duzhong(Eucommia ulmoides oliver), and is also considered as the main bioactive metabolite of polyphenol against oxidative stress and inflammation. This study aimed to investigate the effect of PCA on grow.h performance, intestinal barrier function, and gut microbiota in a w.aned piglet model challenged w.th lipopolysaccharide(LPS).Methods: Thirty-six piglets(Pig Improvement Company line 337 × C48, 28 d of age, 8.87 kg ± 0.11 kg Bw. w.re randomly allocated into 3 treatments and fed w.th a basal diet(CTL), a diet added 50 mg/kg of aureomycin(AUR), or a diet supplemented w.th 4000 mg/kg of PCA, respectively. The piglets w.re challenged w.th LPS(10 μg/kg Bw. on d 14 and d 21 by intraperitoneal injection during the 21-d experiment. Animals(n = 6 from each group) w.re sacrificed after being anesthetized by sodium pentobarbital at 2 h after the last injection of LPS. The serum w.s collected for antioxidant indices and inflammatory cytokines analysis, the ileum w.s harvested for detecting mRNA and protein levels of tight junction proteins by PCR and immunohistochemical staining, and the cecum chyme w.s collected for intestinal flora analysis using 16 S rRNA gene ***: Dietary supplementation of PCA or AUR significantly increased the expression of tight junction proteins including ZO-1 and claudin-1 in intestinal mucosa, and decreased the serum levels of thiobarbituric acid reactive substances(TBARS) and IL-6, as compared w.th CTL group. In addition, PCA also decreased the serum levels of IL-2 and TNF-α(P < 0.05). Analysis of gut microbiota indicated that PCA increased the Firmicutes/Bacteroidetes ratio(P < 0.05). Spearman's correlation analysis at the genus level revealed that PCA reduced the relative abundance of Prevotella 9, Prevotella 2, Holdemanella, and Ruminococcus t

关键词： Gut microbiota Inflammation Piglets Protocatechuic acid Tight junction proteins

Ethanol induced mitochondria injury and permeability transition pore opening: Role of mitochondria in alcoholic liver disease

在线全文

学校读者我要写书评

暂无评论

w.rld Journal of Gastroenterology 2007年第16期13卷 2352-2356页

作者： Ming Yan Ping Zhu Hui-Min liu Hai-Tao Zhang Li liu Department of Digestive Diseases Qilu Hospital Shandong University Jinan 250012 Shandong Province China Jinan Infectious Disease Hospital Jinan 250012 ShandongProvince China

AIM: To observe changes of mitochondria and investigate the effect of ethanol on mitochondrial perme- ability transition pore (PTP), mitochondrial membrane potential (MMP, ΔΨm) and intracellular calcium concentration in hepatocytes by establishing an animal model of alcoholic liver disease (ALD). METHODS: Fourty adult male w.star rats w.re randomly divided into tw. groups, the model group (20) w.s administered alcohol intragastrically plus an oliver oil diet to establish an ALD model, and the control group (20) w.s given an equal amount of normal saline. The ultramicrostructural changes of mitochondria w.re observed under electron microscopy. Mitochondria of liver w.s extracted, and patency of PTP, mitochondrial membrane potential (ΔΨm), mitochondrial mass and intracellular calcium concentration of isolated hepacytes w.re detected by flow.cytometry using rhodamine123 (Rh123), Nonyl-Acridine Orange and calcium fluorescent probe Fluo-3/AM, respectively. RESULTS: Membrane and cristae w.re broken or disappeared in mitochondria in different shapes under electron microscopy. Some mitochondria show.d U shape or megamitochondrion. In the model group, liver mitochondria PTP w.s broken, and mitochondria sw.lled, the absorbance at 450 nm, A540 decreased (0.0136 ± 0.0025 vs 0.0321 ± 0.0013, model vs control, P w.s low.red; mitochondrial mass (17.4350 ± 1.9880 vs 31.6738 ± 3.4930, P w.s increased in liver cells (7.0020 ± 0.5008 vs 10.2050 ± 0.4701, P < 0.01).CONCLUSION: Chronic alcohol intake might lead to broken mitochondria PTP, decreased mitochondria membrane potential and injury, and elevated intracellular Ca2+ production. Ethanol-induced chondriosome injury may be an important mechanism of alcoholic diseases.

关键词： Alcoholic liver disease Chondriosome Apoptosis Ultra microstructure Membrane potentials Permeability transition pore Transmembrane potential chondriosome mass Ca^2+

Pharmacokinetic study about compatibility of Eucommia ulmoides and Psoralea corylifolia

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Herbal Medicines 2023年第2期15卷 263-270页

作者： Yuxing Huang Erw.i liu Xuhua Huang Jia Hao Siyuan Hu Xiumei Gao Tianjin University of Traditional Chinese Medicine Tianjin 301617China Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis Tianjin University of Traditional Chinese MedicineTianjin 301617China Tianjin Key Laboratory of Chinese Medicine Pharmocology Tianjin University of Traditional Chinese MedicineTianjin 301617China First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin 300193China National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion Tianjin 300193China

Objective:The compatibility of Eucommia ulmoides(Eu)and Psoralea corylifolia(Pc)on the pharmacokinetic(PK)properties in the rat w.s explored in this ***:Eu extract,Pc extract and the combined extracts(crude drug ratio w.s 2:1)w.s administered by gavage,*** PK experiments w.re *** first one,the blood samples w.re collected via the occuli chorioideae vein to get the PK properties of the *** second one,the blood samples w.re simultaneously collected via the internal jugular vein or portal vein at different time points and the concentrations of target ingredients w.re detected by LC/MS/MS to clear the location w.ere the interaction of Eu and Pc took place in ***:Eight of 11 ingredients in Eu and Pc extract w.re determined in rat *** exposure levels of geniposidic acid(GPA),aucubin(AU),geniposide(GP),pinoresinol diglucoside(PDG),psoralen glycosides(PLG)and isopsoralen glycosides(IPLG)w.re decreased 1/5–2/3 after administration of combined *** to the combined administration,the exposure of GPA and AU in plasma of single Eu administration collected via the portal vein w.re decreased 1/3–2/3,and the values of AUC0-24h and AUC0-∞ of GP collected from the portal vein or internal jugular vein w.re double *** other components’parameters w.re not significantly ***:In summary,the Pc and Eu combined administration could affect the exposure of the main components of Eu extract in rats due to the changed intestinal *** research on the compatibility of Pc and Eu w.s helpful to guide the clinical administration of Eu and Pc simultaneously.

关键词： double intubation drug interaction Eucommia ulmoides oliver LC/MS/MS pharmacokinetic properties Psoralea corylifolia L

在线全文

学校读者我要写书评

暂无评论

全球竹建筑概述——趋势和挑战

世界建筑 2013年第12期 27-34页

作者：刘可为奥利弗.弗里斯国际竹藤组织(INBAR)

笔者回顾了世界各地竹建筑的历史,讲述了竹材同其他建筑材料,诸如天然泥土/石灰、近代混凝土/水泥、胶粘剂之间的关系。竹子是世界上生长速度最快的植物,具有优良的结构性能,但在建筑中的潜力还未被充分挖掘。本文在描述世界各地竹建筑... 详细信息

笔者回顾了世界各地竹建筑的历史,讲述了竹材同其他建筑材料,诸如天然泥土/石灰、近代混凝土/水泥、胶粘剂之间的关系。竹子是世界上生长速度最快的植物,具有优良的结构性能,但在建筑中的潜力还未被充分挖掘。本文在描述世界各地竹建筑现状的同时,指出了目前竹建筑产业所面临的挑战,并就进一步的研究和发展提出了一些建议。

关键词：竹子原竹建筑竹编墙技术工程竹重组竹结构用竹篾层积材结构用胶合竹