Eucommia lignans alleviate the progression of diabetic nephropathy through mediating the AR/Nrf2/HO-1/AMPK axis in vivo and in vitro

作     者：HUANG Qi ZHANG Yinfan JIANG Yueping HUANG Ling LIU Qiong OUYANG Dongsheng HUANG Qi;ZHANG Yinfan;JIANG Yueping;HUANG Ling;LIU Qiong;OUYANG Dongsheng

作者机构：Department of PharmacyXiangya HospitalCentral South UniversityChangsha 410008China National Clinical Research Center for Geriatric DisordersXiangya HospitalCentral South UniversityChangsha 410008China Hunan Key Laboratory for Bioanalysis of Complex Matrix SamplesChangsha Duxact Biotech Co.Ltd.Changsha 411000 China Department of Operating RoomXiangya HospitalCentral South UniversityChangsha 410008China Department of OncologyXiangya HospitalCentral South UniversityChangsha 410008China Department of Clinical PharmacologyXiangya HospitalCentral South UniversityChangsha 410008China Institute of Clinical PharmacologyHunan Key Laboratory of PharmacogeneticsCentral South UniversityChangsha 410028China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2023年第21卷第7期

页      面：516-526页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 100706[医学-药理学] 1002[医学-临床医学] 0703[理学-化学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the National Development of Key Novel Drugs for Special Projects of China(No.2017ZX09304014) the National Natural Science Foundation of China(No.82003929) Hunan Provincial Natural Science Foundation of China(No.2021JJ40993) Hunan Key Laboratory for Bioanalysis of Complex Matrix Samples(No.2017TP1037) 

主　　题：Diabetic nephropathy(DN) Eucommia lignans Aldose reductase(AR) Nrf2/HO-1/AMPK 

摘      要：Lignans derived from Eucommia ulmoides Oliver(Eucommia lignans)inhibit the progression of inflammatory diseases,while their effect on the progression of diabetic nephropathy(DN)remained *** work was designed to assess the function of Eucommia lignans in *** major constituents of Eucommia lignans were analyzed by UPLC-Q-TOF-MS/*** binding between Eucommia lignans and aldose reductase(AR)was predicted by molecular *** lignans(200,100,and 50 mg·kg^(-1))were used in model animals to evaluate their renal function *** glomerular mesangial cells(HBZY-1)were transfected with sh-AR,sh-AMPK,and oe-AR in the presence of high glucose(HG)or HG combined with Eucommia lignans to evaluate whether Eucommia lignans affected HG-induced cell injury and mitochondrial dysfunction through the AR/Nrf2/HO-1/AMPK *** lignans significantly attenuated the progression of DN in *** lignans notably reversed HG-induced upregulation of inflammatory cytokines and mitochondrial injury,while downregulating the levels of Cyto c,caspase 9,AR,and NOX4 in HBZY-1 *** contrast,HG-induced downregulation of Nrf2,HO-1 and p-AMPKαlevels were abolished by Eucommia ***,knockdown of AR exerted similar therapeutic effect of Eucommia lignans on DN progression,and AR overexpression reversed the effect of Eucommia *** lignans alleviated renal injury through the AR/Nrf2/HO-1/AMPK ***,these findings might provide evidence for the use of Eucommia lignans in treating DN.