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The mechanism study on silica nanoparticles-induced blood hypercoagulable state and thrombus formation

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The mechanism study on silica nanoparticles-induced blood hy...

中国毒理学会第七次全国会员代表大会暨中国毒理学会第六次中青年学者科技论坛

作者： junchao duan Yang Yu Yang Li Yanbo Li Xianqing Zhou Zhiwei Sun Department of Toxicology and Sanitary Chemistry School of Public Health Capital Medical University Beijing Key Laboratory of Environmental Toxicology Capital Medical University

Silica nanoparticles (Si NPs) are widely applied in food sciences, industry and biomedicine, which greatly increase the human exposure and potential health risk. However, the effect of Si NPs on circulation system, especially on thrombus formation was largely *** study was aimed to explore the adverse effect of Si NPs on thrombus formation, and to elucidate the underlying mechanisms in zebrafish (Danio rerio). Using transgenic lines,Tg(mpo:GFP) and Tg(fli-1:EGFP) zebrafish, the activation of neutrophils-mediated inflammation and impairment of vascular endothelium were detected in caudal vein of zebrafish induced by Si NPs. The blood flow and blood velocity decreased significantly via ZebraBlood analysis,reflecting the haemodynamic changes triggered by Si NPs. The thrombus formation in Si NPstreated zebrafish embryos was markedly increased by erythrocytes staining. Based on microarray and bioinformatics analysis, JAK1/TF/PAR1 signaling pathway was verified by qRT-PCR and Western blot assay. For in-deep study, the il6st and f2r were knocked down with specific morpholinos (MO). Whole-mount in situ hybridization and qRT-PCR analysis showed that the expression jak1 and f3b were attenuated in il6st knockdown groups;while knockdown of f2r effective declined the fga expression induced by Si NPs. Knockdown of f2r also caused the inhibition of fibrinogen expression and thrombus formation. In summary, our data demonstrated that Si NPs induced inflammation-coagulation cascade, blood hypercoagulable state and thrombus formation via JAK1/TF/PAR1 signaling pathway;il6st and f2r are critical molecular targets forSi NPs-induced thrombus formation.

关键词： Silica nanoparticles inflammation-coagulation cascade thrombus formation

Intravenously injection of silica nanoparticles induces liver injury in mice and related mechanism study

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Intravenously injection of silica nanoparticles induces live...

中国毒理学会第七次全国毒理学大会暨第八届湖北科技论坛

作者： Yang Yu junchao duan Yang Li Li Jing Man Yang Ji Wang Yanbo Li Zhiwei Sun School of Public Health Capital Medical University Beijing Key Laboratory of Environmental Toxicology Capital Medical University

As Silica nanoparticles(SiNPs) have been widely used in the biomedical and pharmaceutical fields,the adverse effects and toxicity research of SiNPs received more *** the biomedical application,intravenous injection is the most common exposure route for SiNPs,which make the liver one of the major target organs,as the main compartment of mononuclear phygocyte system(MPS) for scavenging blood borne *** this regard,the evaluation of hepatic toxicity of SiNPs was performed in vivo in this *** indicated SiNPs could accumulate in almost all the types of cells in liver and induced hepatocellular injury and granuloma formation in liver in SiNPs treated *** hepatocyte apoptosis was visualized both under the microscope and TEM,and verified by the TUNEL *** increase of lipid peroxide level and decrease of antioxidant enzyme activities in liver suggested SiNPs induced hepatic oxidative *** results of Masson’s trichrome stain and hydroxyproline assay showed collagen fibers hyperplasia in liver,suggesting SiNPs could cause liver fibrosis by intravenous injection in mice,the elevation of expression of TGF-β/Smad3 along with the liver fibrosis proceeding indicating TGF-β/Smad3 played important roles in SiNPs inducing liver *** study may provide useful hints for SiNPs safety evaluation in the biomedical and pharmaceutical application.

关键词： Silica nanoparticles Repeated intravenous injection Oxidative damage Apoptosis Fibrosis Liver

基于建筑信息模型技术的城市轨道交通站后工程设计优化应用研究

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城市轨道交通研究 2021年第7期24卷 204-207页

作者：段军朝贾锐奇中建三局基础设施建设投资有限公司武汉430061 中建三局轨道交通公司武汉430061 中建三局集团有限公司武汉430064 中建三局安装工程有限公司武汉430064

站后工程属于城市轨道交通项目施工过程中最为复杂、接口数量最为庞大的工程,其建设质量直接影响列车的运行安全。以成都轨道交通6号线三期工程为研究案例,对BIM(建筑信息模型)技术在站后工程设计中的各项优化进行探索,在体系建设、施... 详细信息

站后工程属于城市轨道交通项目施工过程中最为复杂、接口数量最为庞大的工程,其建设质量直接影响列车的运行安全。以成都轨道交通6号线三期工程为研究案例,对BIM(建筑信息模型)技术在站后工程设计中的各项优化进行探索,在体系建设、施工图协同设计、设计优化输出等方面进行了具体的分析与研究。研究表明,采用BIM技术进行设计优化,可实现设计与施工的高效协同,大幅提高设计效率,并有效提高施工图设计质量。

关键词：城市轨道交通站后工程建筑信息模型设计优化

Toxic effect of low dose co-exposure with silica nanoparticles and benzo[a]pyrene on cardiaovascular system in zebrafish embryos

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Toxic effect of low dose co-exposure with silica nanoparticl...

中国毒理学会第七次全国毒理学大会暨第八届湖北科技论坛

作者： junchao duan Yang Yu Yang Li Li Jing Man Yang Ji Wang Yanbo Li Zhiwei Sun School of Public Health Capital Medical University Beijing Key Laboratory of Environmental Toxicology Capital Medical University

Given the severe situation of world-wide particulate matter air pollution,it is urgent to explore the combined biological effects of multiple components on particulate *** zebrafish model,this study was aimed to determine whether the co-exposure to silica nanoparticles（SiNPs） and benzo[a]pyrene（B[a]P） had a pronounced cardiovascular toxicity than the single *** FTIR and TGA analysis showed the co-exposure system had high absorption and thermal *** exposed to SiNPs or B[a]P alone did not show cardiac toxicity ***,embryos co-exposed to SiNPs and B[a]P exhibited pericardial edema and *** ROS generation remained unaffected,the co-exposure induced significant inflammatory response and caused erythrocyte aggregation in *** analysis showed that the co-exposure of SiNPs and B[a]P significantly enhanced the expression of proinflammatory and procoagulant *** study suggested the cardiovascular toxicity was triggered by the combinated effects of SiNPs and B[a]P at low dose exposure.

关键词： Benzo[a]pyrene Silica nanoparticles Cardiovascular toxicity Co-exposure Zebrafish

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饰面清水混凝土双曲面桥墩施工技术

施工技术 2008年第6期37卷 60-62页

作者：段军朝王树峰中建三局工程总承包公司湖北武汉430070

武广客运专线新建武汉站站房高架桥桥墩设计为双曲变截面,在建筑外观设计中突破了传统的平面饰面清水混凝土设计理念,而突显双曲面的饰面清水效果,以达到与梁体饰面清水混凝土浑然一体的装饰效果。重点从模板加工与安装、钢筋绑扎、混... 详细信息

武广客运专线新建武汉站站房高架桥桥墩设计为双曲变截面,在建筑外观设计中突破了传统的平面饰面清水混凝土设计理念,而突显双曲面的饰面清水效果,以达到与梁体饰面清水混凝土浑然一体的装饰效果。重点从模板加工与安装、钢筋绑扎、混凝土配合比设计和浇筑等几个方面介绍饰面清水混凝土双曲面桥墩施工技术。

关键词：模板工程钢筋工程饰面清水混凝土双曲面桥墩

Fine particulate matters inducing reproductive impairment by causing cell cycle arrest and activating mitochondrial apoptosis pathway in male rats and spermatocyte cells

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Fine particulate matters inducing reproductive impairment by...

中国生理学会内分泌代谢、比较生理与应激生理学术会议

作者： Jianhui Liu Lihua Ren Jialiu Wei Jin Zhang Yannan Zhang Hejing Hu junchao duan Zhiwei Sun Xianqing Zhou Department of Toxicology and Hygienic Chemistry School of Public HealthCapital Medical University Beijing Key Laboratory of Environmental Toxicology Capital Medical University

Fine particulate matters(PM2.5) have been shown to induce male reproductive toxicity,but the mechanism is *** explore the toxic mechanism of PM2.5,32 male Sprague-Dawley(SD)rats were treated to normal saline(control group) or PM2.5 with the doses of 1.8,5.4 and 16.2 mg/*** intratracheal instillation every 3 days for 30 *** 30 days,the rats were sacrificed for tissue *** vitro,GC-2 spd cells were treated to various concentrations(12.5,25,50 μg/mL) of PM2.5 for 24 *** results showed that PM2.5 obviously decreased sperm quality,increased sperm abnormality rate and damaged testicular tissue in male ***2.5 induced oxidative stress and DNA damage in ***,PM2.5 significantly increased the expression of BAX,cytochrome C,Caspase-9,Caspase-3 on the 30 th *** vitro,GC-2 cells treated with PM2.5 displayed mitochondria swelling and cristae ***2.5 induced S-phase cell cycle arrest and apoptosis in GC-2 spd *** results suggest that PM2.5 might induce apoptosis by up-regulating the expressions of mitochondrial apoptosis pathway factors through causing DNA damage and cell cycle arrest resulting from oxidative stress,and cause a decrease in the quantity and quality of sperms.

关键词： Fine particulate matters oxidative stress cell cycle arrest mitochondrial apoptosis pathway male reproduction

Metabolic Impact Induced by Total,Water Soluble and Insoluble Components of PM2.5 Acute Exposure in Mice

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Metabolic Impact Induced by Total,Water Soluble and Insolubl...

2017环境与公共健康学术会议暨中国环境科学学会环境医学与健康分会、中国毒理学会生化与分子毒理专业委员会2017年年会

作者： Yannan Zhang Yanbo Li Zhixiong Shi Jing Wu Xiaozhe Yang Lin Feng Lihua Ren junchao duan Zhiwei Sun Department of Toxicology and Sanitary Chemistry School of Public HealthCapital Medical University Beijing Key Laboratory of Environmental Toxicology Capital Medical University

The systemic biological effects on metabolic responses induced by PM and its components were poorly *** study was aimed to evaluate the toxicity of different components of PM via metabolomics *** the present study,we adoptedH NMR-based metabolomics approach to evaluate metabolic profiles in mice after acute exposure to Total-PM,water soluble

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Silica nanoparticles exposure inducing reproductive toxicity by DNA damage and mitochondrial apoptosis pathway in female Balb/c mice

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Silica nanoparticles exposure inducing reproductive toxicity...

第十三届全国野生动物生态与资源保护学术研讨会暨第六届中国西部动物学学术研讨会

作者： Jianhui Liu Man Yang Li Jing Lihua Ren Jialiu Wei Jin Zhang Feng Zhang junchao duan Xianqing Zhou Zhiwei Sun Department of Toxicology and Hygienic Chemistry Capital Medical University Beijing Key Laboratory of Environmental Toxicology Capital Medical University College of life science Qilu normal Normal University

Given that the effects of ultrafine fractions(<0.1μm)on reproductive diseases are gaining attention,this study aimed to explore the influence of silica nanoparticles(SiN Ps)-induced female reproductive *** this study,80 female mice were randomly divided into four groups including a control group and three concentrations of SiNPs

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地铁暗挖隧道下穿预混站相互影响评价分析

西安工业大学学报 2021年第4期41卷 407-413页

作者：杨正华段军朝徐杰西安工业大学建筑工程学院西安710021 中建三局基础设施建设投资有限公司武汉430061 中铁第一勘察设计院集团有限公司西安710043

为了评估成都地铁6号线三期出入场线下穿预混站的安全性,采用数值计算方法,利用Midas软件建立三维有限元模型,对隧道开挖施工、预混站的加卸载和后期预混站的拆除进行了数值仿真分析,得出了隧道开挖对地面沉降、上部既有结构变形和内力... 详细信息

为了评估成都地铁6号线三期出入场线下穿预混站的安全性,采用数值计算方法,利用Midas软件建立三维有限元模型,对隧道开挖施工、预混站的加卸载和后期预混站的拆除进行了数值仿真分析,得出了隧道开挖对地面沉降、上部既有结构变形和内力的影响程度及规律,给出了预混站设计寿命中的各工况对地铁隧道的影响。结合分析,提出了工程实施中的主要监测项目和控制指标,并将相关工程监测数据和数值分析结果进行了对比分析,结果表明:隧道开挖工程中引起的土体扰动和应力重分布会对上部预混站桩基产生卸载效应;整体引起的既有预混站桩基轴力及弯矩变化较小,均在工程可接受的范围内。

关键词：地铁隧道预混站地面沉降