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Comprehensive Analysis of Expressed Sequence Tags from the Pulp of the Red Mutant ‘Cara Cara’ Navel Orange (Citrus sinensis Osbeck)

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Journal of Integrative Plant Biology 2010年第10期52卷 856-867页

作者： jun-li ye An-Dan Zhu Neng-Guo Tao Qiang Xu Juan Xu Xiu-Xin Deng National Key Laboratory of Crop Genetic Improvement National Center of Citrus Breeding Huazhong Agricultural University Wuhan430070 China

Expressed sequence tag （EST） analysis of the pulp of the red-fleshed mutant ＇Cara Cara＇ navel orange provided a starting point for gene discovery and transcriptome survey during citrus fruit maturation. interpretation of the EST datasets revealed that the mutant pulp transcriptome held a high section of stress responses related genes, such as the type III metallothionein-like gene （6.0%）, heat shock protein （2.8%）, Cu/Zn superoxide dismutase （0.8%）, late embryogenesis abundant protein 5 （0.8%）, etc. 133 transcripts were detected to be differentially expressed between the red mutant and its orange-color wild genotype ＇Washington＇ via digital expression analysis. Among them, genes involved in metabolism, defense/stress and signal transduction were statistical overrepresented. Fifteen transcription factors, composed of NAM, ATAF, and CUC transcription factor （NAC）; myeloblastosis （MYB）; myelocytomatosis （MYC）; basic helix-loop-helix （bHLH）; basic leucine zipper （bZIP） domain members, were also included. The data reflected the distinct expression profile and the unique regulatory module associated with these two genotypes. Eight differently expressed genes analyzed in digital were validated by quantitative real- time polymerase chain reaction. For structural polymorphism, both simple sequence repeats and single nucleotide polymorphisms （SNP） loci were surveyed; dinucleotide presentation revealed a bias toward AG/GA/TC/CT repeats （52.5%）, against GC/CG repeats （0%）. SNPs analysis found that transitions （73%） outnumbered transversions （27%）. Seventeen potential cultivar-specific and 387 heterozygous SNP loci were detected from ＇Cara Cara＇ and ＇Washington＇ EST pool.

关键词： Surveys Surveying Sweet oranges Genes Pulp Pulping Mutants Gas chromatography Transcription factors Copper

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Cloning and Characterization of a Novel cDNA Encoding Late Embryogenesis-Abundant Protein 5 like (LEA-5) Gene from Cara Cara Navel Orange Fruit (Citrus sinensis Osbeck)

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Agricultural Sciences in China 2006年第4期5卷 272-276页

作者： TAO Neng-guo ye jun-li XU Juan DENG Xiu-xin National Key Laboratory of Crop Genetic Improvement National Center of Crop Molecular Breeding Huazhong Agricultural University Wuhan 430070 P.R.China

LEA5 gene was postulated related with both stress and hormone responses. In an attempt to find genes exclusively expressed during fruit ripening of Cara Cara navel orange, a novel cDNA clone encoding late embryogenesis-abundant protein 5 like gene （CitLEA5-1） was obtained. It was 582 bp in length, containing 97 deduced amino acids. Compared with the stress-induced LEA5 from leaves of Citrus sinensis, CitLEA5-1 had a shorter 3′ untranslated region （UTR）. Semi-quantitative RT-PCR analysis revealed that CitLEA5-1 was transcriptional regulated during fruit ripening of Cara Cara navel orange.

关键词： Citrus cloning characterization late embryogenesis-abundant protein

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Two Lycopene β-Cyclases Genes from Sweet Orange (Citrus sinensis L. Osbeck) Encode Enzymes With Different Functional Efficiency During the Conversion of Lycopene-to-Provitamin A

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Journal of Integrative Agriculture 2013年第10期12卷 1731-1747页

作者： ZHANG Jian-cheng ZHOU Wen-jing XU Qiang TAO Neng-guo ye jun-li GUO Fei XU Juan DENG Xiu-xin Key Laboratory of Horticultural Plant Biology Ministry of Education/College of Horticulture and ForestryHuazhong Agricultural University

Citrus fruits are rich in *** the carotenoid biosynthetic pathway,lycopene β-cyclase（LCYb,EC：1.14.-.-） is a key regulatory enzyme in the catalysis of lycopene to β-carotene,an important dietary precursor of vitamin A for human *** closely related lycopene β-cyclase cDNAs,designated CsLCYb1 and CsLCYb2,were isolated from the pulp of orange fruits（Citrus sinensis）.The expression level of CsLCYb genes is lower in the flavedo and juice sacs of a lycopeneaccumulating genotype Cara Cara than that in common genotype Washington,and this might be correlated with lycopene accumulation in Cara Cara *** CsLCYb1 efficiently converted lycopene into the bicyclic β-carotene in an Escherichia coli expression system,but the CsLCYb2 exhibited a lower enzyme activity and converted lycopene into the β-carotene and the monocyclic γ-*** tomato transformation studies,expression of CsLCYb1 under the control of the cauliflower mosaic virus（CaMV） 35S constitutive promoter resulted in a virtually complete conversion of lycopene into β-carotene,and the ripe fruits displayed a bright orange ***,the CsLCYb2 transgenic tomato plants did not show an altered fruit colour during development and *** fruits of the CsLCYb1 transgenic plants,most of the lycopene was converted into β-carotene with provitamin A levels reaching about 700 μg ***,most transgenic tomatoes showed a reduction in total carotenoid accumulation,and this is consistent with the decrease in expression of endogenous carotenogenic genes in transgenic ***,these results suggested that the cloned CsLCYb1 and CsLCYb2 genes encoded two functional lycopene β-cyclases with different catalytic efficiency,and they may have potential for metabolite engineering toward altering pigmentation and enhancing nutritional value of food crops.

关键词： carotenoids biosynthesis citrus fruit gene expression lycopene lycopene β-cyclase β-Carotene transgenic tomato fruit

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Growth and structural characteristics of metastableβ-In2Se3 thin films on H-terminated Si(111)substrates by molecular beam epitaxy

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Chinese Physics B 2020年第5期29卷 429-434页

作者： Yi-Fan Shen Xi-Bo Yin Chao-Fan Xu Jing He jun-ye li Han-Dong li Xiao-Hong Zhu Xiao-Bin Niu College of Materials Science and Engineering Sichuan UniversityChengdu 610064China School of Materials and Energy University of Electronic Science and Technology of ChinaChengdu 610054China

Epitaxial growth and structural characteristics of metastableβ-In2Se3 thin films on H-terminated Si(111)substrates are *** In2Se3 thin films grown below theβ-to-αphase transition temperature(453 K)are characterized to be strainedβ-In2Se3 mixed with significantγ-In2Se3 *** pure-phased single-crystallineβ-In2Se3 can be reproducibly achieved by in situ annealing the as-deposited poly-crystalline In2Se3 within the phase equilibrium temperature window ofβ-*** is suggeted that the observedγ-to-βphase transition triggered by quite a low annealing temperature should be a rather lowered phase transition barrier of the epitaxy-stabilized In2Se3 thin-film system at a state far from thermodynamic equilibrium.

关键词： In2Se3 molecular beam epitaxy single-crystalline annealing and quench phase transition

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Partial AZFc duplications not deletions are associated with male infertility in the Yi population of Yunnan Province,China

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第9期14卷 807-815页

作者： jun-jie ye li MA li-juan YANG Jin-huan WANG Yue-li WANG Hai GUO Ning GONG Wen-hui NIE Shu-hua ZHAO Yunnan Key Laboratory of Fertility Regulation and Minority Eugenics Yunnan Population and Family Planning Research Institute State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of Sciences Department of Urology Kunming General Hospital of Chengdu Military Command Haiyuan College Kunming Medical University

Objective:There are many reports on associations between spermatogenesis and partial azoospermia factor c(AZFc) deletions as well as duplications;however,results are conflicting,possibly due to differences in methodology and ethnic *** purpose of this study is to investigate the association of AZFc polymorphisms and male infertility in the Yi ethnic population,residents within Yunnan Province,ChinaMethods:A total of 224 infertile patients and 153 fertile subjects were selected in the Yi ethnic *** study was performed by sequence-tagged site plus/minus(STS+/) analysis followed by gene dosage and gene copy definition analysis.Y haplotypes of 215 cases and 115 controls were defined by 12 binary markers using single nucleotide polymorphism on Y chromosome(Y-SNP) multiplex assays based on single base primer extension ***:The distribution of Y haplotypes was not significantly different between the case and control *** frequencies of both gr/gr(7.6% vs.8.5%) and b2/b3(6.3% vs.8.5%) deletions do not show significant ***,single nucleotide variant(SNV) analysis shows no significant difference of gene copy definition between the cases and ***,the frequency of partial duplications in the infertile group(4.0%) is significantly higher than that in the control group(0.7%).Further,we found a case with sY1206 deletion which had two CDY1 copies but removed half of DAZ ***:Our results show that male infertility is associated with partial AZFc duplications,but neither gr/gr nor b2/b3 deletions,suggesting that partial AZFc duplications rather than deletions are risk factors for male infertility in Chinese-Yi population.

关键词： Azoospermia factor c(AZFc) AZFc polymorphism b2/b3 gr/gr Infertility

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乌饭树叶黄酮对糖尿病小鼠胰岛形态和功能的影响

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中国医院药学杂志 2020年第12期40卷 1345-1348,1389页

作者：叶俊丽梁韬浙江大学医学院附属儿童医院药剂科、国家儿童健康与疾病临床医学研究中心浙江杭州310003 广西医科大学附属口腔医院广西南宁530021

目的:探讨乌饭树叶黄酮对链脲佐菌素诱导的糖尿病小鼠的胰岛形态和功能的影响。方法:采用昆明小鼠作为研究对象,以静脉注射链脲佐菌素致小鼠体内诱导形成糖尿病模型。造模成功后将糖尿病小鼠随机分为模型对照组、二甲双胍对照组、80 mg... 详细信息

目的:探讨乌饭树叶黄酮对链脲佐菌素诱导的糖尿病小鼠的胰岛形态和功能的影响。方法:采用昆明小鼠作为研究对象,以静脉注射链脲佐菌素致小鼠体内诱导形成糖尿病模型。造模成功后将糖尿病小鼠随机分为模型对照组、二甲双胍对照组、80 mg·kg^-1·d^-1和160 mg·kg^-1·d^-1乌饭树叶黄酮组,另设有空白对照组。随后各组小鼠连续给予相应药物14 d。末次给药后,各组小鼠经过夜禁食,测其空腹血糖水平,而后收集小鼠血清和胰腺标本。EliSA法检测血清胰岛素;胰腺组织制备匀浆用于超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)的活性和丙二醛(malondialdehyde,MDA)含量;测定胰腺组织中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和白细胞介素6(interleukin 6,IL-6)水平;并以HE染色及透射电镜观察胰岛组织病理学和超微结构变化。结果:与模型组相比较,糖尿病小鼠经乌饭树叶黄酮干预后空腹血糖有不同程度下降(P<0.05);乌饭树叶黄酮受试组的血清胰岛素含量明显比模型对照组高(P<0.05);与模型对照组比较,乌饭树叶黄酮组的胰腺组织中SOD和GSH-Px活性显著升高,MDA含量则明显下降,与此同时,炎症因子TNF-α和IL-6也明显下降。糖尿病小鼠的胰腺组织病理形态学和超微机构改变也在经乌饭树叶黄酮干预后有所改善。结论:乌饭树叶黄酮可降低糖尿病小鼠血糖,该作用可能与其能改善糖尿病小鼠血脂代谢和胰腺组织的氧化应激状态进而减少炎症反应有关。

关键词：乌饭树叶黄酮糖尿病血脂氧化应激炎症因子

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Effect of cholesterol metabolism on hepatolithiasis

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World Journal of Gastroenterology 2025年第1期31卷 157-162页

作者： lin Zheng Zi-Yu ye jun-Ji Ma Department of Gastroenterology The Second Hospital of Hebei Medical UniversityHebei Key Laboratory of GastroenterologyHebei Institute of GastroenterologyHebei Clinical Research Center for Digestive DiseasesNo.215 Heping West RoadShijiazhuang 050000Hebei ProvinceChina

Surgical intervention is currently the primary treatment for hepatolithiasis;how-ever,some patients still experience residual stones and high recurrence rates after *** metabolism seems to play an important role in hepatoli-thiasis pathogenesis.A high cholesterol diet is one of the significant reasons for the increasing incidence of ***,regular diet and appropriate medical intervention are crucial measures to prevent hepatolithiasis and reduce recurrence rate after *** dietary cholesterol and drugs that increase cholesterol stone solubility are key therapeutic approaches in treating *** article discusses the cholesterol metabolic pathways related to the pathogenesis of hepatolithiasis,as well as food intake and targeted therapeutic drugs.

关键词： Hepatolithiasis Cholesterol metabolism High-fat diet 3-hydroxy-3-methylglutaryl-coenzyme A reductase Interlobular bile duct

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Effect of two human growth hormone receptor antagonists on glomerulosclerosis in streptozotocin-induced diabetic rats

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Acta Pharmacologica Sinica 2004年第4期25卷 92-97页

作者： Wei li, li-hua ZHU, En-bi WANG, Zeng-can ye, jun liN, li-he GUO1, Fei-hong LUO2, Xi-hong liU2, Xin FANG2, Shui-xian SHEN2 Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Science, Chinese Academy of Sciences, 2Children’s Hospital ofShanghaiMedical University, Shanghai200031, China Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Science Chinese Academy of Sciences Children's Hospital of Shanghai Medical University 上海 200031

AIM: To explore the feasibility of human growth hormone (hGH) receptor antagonist in the treatment of end-stage diabetic renal complications. METHODS: Two hGH mutants, hGHA1 (Cys-hGH-del1-4, G120R, K168A, E174A, C182S, del186-191) and hGHA2 (hGH-H21A, G120R, E174A) were expressed in E coli. The IC50 (Mean±SD) values for the mutants for inhibiting 125I-hGH binding to rabbit growth hormone receptor were (65±10) ng for hGHA1, (27±5.6) ng for hGHA2, and (10±0.6) ng for wild type hGH, respectively. RESULTS: After treatment for 12 weeks, the renal histology analysis showed that treatment with hGHA2 at 4 mg/kg body weight daily markedly suppressed glomerulosclerosis in streptozotocin-induced diabetic Sprague-Dawley (SD) rats; hGHA1 at the same dosage slightly increased the renal damage compared with saline; while wild type hGH at 1 U/kg body weight daily severely worsened the glomerulo-sclerosis in diabetic SD rats. CONCLUSION: The data indicated that hGHA2 inhibited the end-stage glomerulosclerosis in diabetic rats, but hGHA1 mildly increased the glomerulosclerosis.

关键词： growth hormone diabetic nephropathies mutation diabetes mellitus

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Phylogenetic Relationship within Dunaliella （Chlorophyta） inferred from nrDNA ITS Data

Phylogenetic Relationship within Dunaliella （Chlorophyta） ...

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中国藻类学会第十一次学术讨论会

作者： Luo li-ming, Hu Hong-jun, li ye-guang, Ouyang ye-xin, li Zhong-kui, Gen Yahong (Institute of Bontany, the Chinese academy of Sciences, Wuhan 430074)

＜正＞ The genus Dunaliella comprises 28 species defined mainly by morphological andphysiological criteria, Which vary considerably depending on growth conditions. The taxonomicstatus of various species is uncertain too. In order to confirm the taxonomic identity and betterunderstand the relationship within Dunaliella, four species and strains of Dunaliella are culturedin our laboratory which were collected and isolated from inner Mongolia. Their internaltranscribed spacers （ITS） sequences of nuclear ribosomal DNA were determined. Still, other twoDunaliella species and Haematococcus droebakensis ITS sequences were obtained from Genbank.

关键词： phylogenetio relationship Dunaliella nrDNA ITS.

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The effects of ciliary neurotrophic factor on neurological function and glial activity following contusive spinal cord injury in the rats

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第二军医大学学报 2004年第5期25卷 546-546页

作者： ye jun-li CAO li CUI Rui-Yao HUANG Ai-jun YAN Zhi-Yong LU Chang-lin HE Cheng Department of Neurobiology Second Military Medical UniversityShanghai 200433 Department of Pathophysiology Qingdao Medical College of Qingdao UniversityQingdao 266021

Ciliary neurotrophic factor (CNTF) has been implicated in the pathophysiology of injury to the central nervous system. The rapid increase in CNTF production following spinal cord injury (SCI) in rats is thought to serve a role in the neuronal survival and functional recovery. In this study, 40 SD rats were divided into four groups.- sham-

关键词： The effects of ciliary neurotrophic factor on neurological function and glial activity following contusive spinal cord injury in the rats

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