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Expression, subcellular localization and protein-protein interaction of four isoforms of EcR/USP in the common cutworm

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Insect Science 2015年第1期22卷 95-105页

作者： li-Xia Huang Yan-jun Gong jun Gu Bao-Juan zeng li-Hua Huang Qi-li Feng Laboratory of Molecular and Developmental Entomology Guangdong Provincial Key Laboratory of Biotechnology for Plant DevelopmentSchool of Life Sciences South China Normal University Guangzhou China

Ecdysone receptor （EcR） and ultraspiracle （USP） form heterodimers to mediate ecdysteroid signaling during molting and metamorphosis. Various EcR/USP heterodimers have been reported. However, it is unclear what kind of EcR/USP combination is adopted by lepidopteran insects during the larval-pupal metamorphosis and whether the EcR/USP heterodimer varies among different tissues. To address these questions, two isoforms of each EcR and USP were cloned from the common cutworm, their messenger RNA expression patterns were examined by real-time quantitative polymerase chain reaction in different tissues during the larval-pupal metamorphosis and in the midgut in response to hormonal induction. Furthermore, their subcellular localization and protein-protein interaction were explored by transient expression and far-western blotting, respectively. All the four genes were significantly up-regulated in prepuae and/or pupae. The expression profiles of EcRB1 and USP1 were nearly identical to each other in the epidermis, fat body and midgut, and a similar situation also applied to EcRA and USP2. The three genes responded to 20-hydroxyecdysone （20E） induction except for USP2, and USP1 could be up-regulated by both 20E and juvenile hormone. The four proteins mainly localized in the nucleus and the nuclear localization was promoted by 20E. The protein-protein interaction between each EcR and USP was found in vitro. These results suggest that two types of EcR/USP heterodimer （EeRA/USP2 and EcRB 1/USP1） may exist simultaneously in the common cutworm, and the latter should play more important roles during the larval-pupal metamorphosis. In addition, the types of EcR/USP heterodimer do not vary in the tissues which undergo histolysis and regeneration during metamorphosis.

关键词： ecdysone receptor protein interaction subcellular localization ultraspiracle

Effects of titanium dioxide nanoparticles on intestinal commensal bacteria

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Nuclear Science and Techniques 2016年第1期27卷 106-110页

作者： li-Ying liu li Sun zeng-Tao Zhong jun Zhu Hai-Yun Song College of life Sciences Nanjing Agriculture University Key Laboratory of Food Safety Research Institute for Nutritional Sciences Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Key Laboratory of Food Safety Risk Assessment Ministry of Health

Nanomaterials and nanotechnology have great potential in the biological and biomedical field. Recent studies reveal that many nanomaterials possess antibacterial activities. While most of these studies focus on the ability of nanomaterials to inhibit the growth of pathogenic bacteria in vitro, few of them test the effects of nanomaterials on intestinal commensal bacteria. Here, we report that Ti O_2nanoparticles(10, 50 and 100 nm in size) can inhibit the growth of Drosophila intestinal commensal bacteria in vitro. This activity depends on the dosage or size, but is independent of the photocatalytic activity of Ti O_2 nanoparticles. Surprisingly, dietary Ti O_2 nanoparticles of the same dosage fail to display similar effects in Drosophila larvae or adults. These flies show a normal amount of intestinal commensal bacteria, as well as a normal developmental cycle, energy store, and locomotor activity. These results imply that the antibacterial effect of Ti O_2 nanoparticles differs in vitro and in vivo.

关键词：二氧化钛纳米颗粒肠道细菌二氧化钛纳米粒子病原菌生长光催化活性纳米材料体外抑制共生细菌

In situ two-step electrochemical preparation of fluoride-free nickel-based compound film on nickel plate for supercapacitors

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Rare Metals 2016年第12期35卷 930-936页

作者： Zhi-Biao Zou Xin-Bo Xiong jun Ma Xie-Rong zeng Tuo Huang jun-Jie li Bin li Shenzhen Key Laboratory of Special Functional Materials Department of Materials Science and EngineeringShenzhen University

A nickel-based compound layer was prepared on a nickel plate by anodization in a 75 wt% H3PO4 solution containing NH4F. This layer was then treated by galvanostatic charge/discharge （GCD） until a black outer layer was detached, leaving behind a film on the nickel plate as a binder-free electrode material for supercapacitors. The microstructural characterization shows that the film consists of Ni（OH）2 and NiO, and no fluoride is found in the as-obtained film. Electrochemical tests demonstrate that this fluoride-free film electrode exhibits a high capacitance of 954 F·g^-1 at 7.5 A·g^-1, excellent rate capability （a 19.5 % capacitance reduction with the current density increasing to 120 A.g-1） and cycling stability. Within 3500 cycles, the specific capacitance does not decrease, but rather increases from 840 F·g^-1 to approximately 1092 F·g^-1 in the first 100 cycles at 60 A·g^-1, and remains stable until the aforementioned layer is detached.

关键词： Nickel Film Anodization Supercapacitor

Asymmetric supercapacitors based on high capacitance Ni6MnO8 and graphene

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Chinese Chemical Letters 2019年第6期30卷 1329-1334页

作者： Mingyang zeng li Zhang Dandan Wu jun Ma Cao Zhang Shujuan li Department of Physics School of Science Lanzhou University of Technology Lanzhou 730050 China Institute for Mathematics Free University of Berlin Berlin D-14195 Germany

Supercapacitors with high energy density and high power density have attracted numerous attentions. Here, we successfully synthesized Ni6MnO8 material by a fast, facile, and cost-effective method, which the area specific capacitance was found to be as high as 1113 mF/cm^2 at a current density of 1 mA/cm^2. Further, the asymmetric supercapacitor was assembled by using Ni6MnO8 as the positive electrode and graphene as the negative electrode with an operation potential from 0 to 1.4 V. It exhibited an area specific capacitance of 69.1m F/cm^2 at 0.5 mA/cm^2 and stable cycling performance which presented about 80% capacitance retention after 5000 cycles at 5 mA/cm^2. The energy density of graphene//Ni6MnO8 supercapacitors was calculated to be 18.81 mWh/cm^2 at a power density of 350.1 mW/cm^2 and still remained 5.8 mWh/cm^2 at a power density of 6990.7 mW/cm^2, meaning that the asymmetric supercapacitor combine excellent power density and energy density.

关键词： Ni6MnO8 Electrode Asymmetric supercapacitors Power density Energy density

Formulation of Optimization Problems with Radiation Field Phases as Design Variables for Pattern Synthesis of linear Antenna Arrays

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China Communications 2022年第12期19卷 86-100页

作者： Fei Zhao jun He Sanyou zeng Changhe li Qinghui Xu Zhigao zeng School of Mechanical Engineering and Electronic Information China University of GeosciencesWuhan 430074China Science and Technology on Blind Signal Processing Laboratory Southwest Electronics and Telecommunication Technology Research InstituteChengdu 610041China School of Automation China University of GeosciencesWuhan 430074China Hubei Key Laboratory of Advanced Control and Intelligent Automation for Complex Systems Wuhan 430074China College of Computer Hunan University of TechnologyZhuzhou CityHunan Province412007China

Pattern synthesise of antenna arrays is usually complicated optimization problems,while evolutionary algorithms(EAs)are promising in solving these *** paper does not propose a new EA,but does construct a new form of optimization *** new optimization formulation has two differences from the common *** is the objective function is the field error between the desired and the designed,not the usual amplitude error between the desired and the *** difference is beneficial to decrease complexity in some *** second difference is that the design variables are changed as phases of desired radiation field within shaped-region,instead of excitation *** difference leads to the reduction of the number of design variables.A series of synthesis experiments including equally and unequally spaced linear arrays with different pattern shape requirements are applied,and the effectiveness and advantages of the proposed new optimization problems are *** results show that the proposing a new optimization formulation with less complexity is as significant as proposing a new algorithm.

关键词： pattern synthesis evolutionary algorithms linear antenna arrays

Yi-Qi-zeng-Min-Tang,a Chinese medicine,ameliorates insulin resistance in type 2 diabetic rats

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World Journal of Gastroenterology 2011年第8期17卷 987-995页

作者： zeng Zhang Hong-li Xue Yi liu Wen-Jian Wang Institute of Chinese Integrative Medicine Huashan Hospital Fudan University Shanghai 200040 China Institute of Chinese Integrative Medicine Huashan Hospital Fudan University Shanghai 200040 China Institute of Integrated Clinical Medicine Shanghai Academy of Traditional Chinese Medicine YueyangIntegrative Medicine Hospital Shanghai Traditional ChineseMedicine University Shanghai 200437 China

AIM:To investigate the effects of the Chinese herbal decoction,Yi-Qi-zeng-Min-Tang(YQZMT),on insulin resistance in type 2 diabetic ***:Sprague-Dawley rats were divided into two dietary regiments by feeding either normal pellet diet(NPD) or high fat diet(HFD).Four weeks later,the HFD-fed rats were injected intraperitoneally with lowdose streptozotocin(STZ).Rats with non-fasting blood glucose level ≥ 16.67 mmol/L were considered type 2 diabetic and further divided into five subgroups:the type 2 diabetes model group,low-dose,medium-doseand high-dose YQZMT groups,and rosiglitazone ***-matched NPD-fed rats served as *** or rosiglitazone were administered for 8 *** glucose and insulin tolerance tests were performed before and after the treatment to measure the glucose tolerance and insulin *** levels of biochemical parameters,adipocytokines,such as tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),as well as free fatty acids(FFAs),were also ***:There was significant elevation of insulin resistance and serum levels of fasting glucose(12.82 ± 1.08 mmol/L vs 3.60 ± 0.31 mmol/L,P lin(7197.36 ± 253.89 pg/mL vs 4820.49 ± 326.89 pg/mL,P lipoprotein cholesterol(LDL-c)(7.84 ± 0.51 mmol/L vs 0.72 ± 0.04 mmol/L,P lipoprotein cholesterol(HDL-c)(0.57 ± 0.03 mmol/L vs 1.27 ± 0.03 mmol/L,P lin resistance,glucose and lipid profile,and reduced levels of FFA,TNF-α and IL-6 in the type 2 diabetic *** the treatment,compared with the diabetic group,the insulin resistance was ameliorated in the high-dose YQZMT(2.82 g/100 g per day) group

关键词： Yi-Qi-zeng-Min-Tang Insulin resistance Type 2 diabetes lipids Adipocytokines Free fatty acids

Investigation of methane adsorption on chlorite by grand canonical Monte Carlo simulations

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Petroleum Science 2017年第1期14卷 37-49页

作者： Jian Xiong Xiang-jun liu li-Xi liang Qun zeng State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum University Institute of Chemical Materials Engineering Physical Academy of China

In this paper, the methane adsorption behaviours in slit-like chlorite nanopores were investigated using the grand canonical Monte Carlo simulation method, and the influences of the pore sizes, temperatures, water, and compositions on methane adsorption on chlorite were discussed. Our investigation revealed that the isosteric heat of adsorption of methane in slit-like chlorite nanopores decreased with an increase in pore size and was less than 42 kJ/mol, suggesting that methane adsorbed on chlorite through physical adsorption. The methane excess adsorp- tion capacity increased with the increase in the pore size in micropores and decreased with the increase in the pore size in mesopores. The methane excess adsorption capacity in chlorite pores increased with an increase in pressure or decrease in pore size. With an increase in temperature, the isosteric heats of adsorption of methane decreased and the methane adsorption sites on chlorite changed from lower- energy adsorption sites to higher-energy sites, leading to the reduction in the methane excess adsorption capacity. Water molecules in chlorite pores occupied the pore wall in a directional manner, which may be related to the van der Waals and Coulomb force interactions and the hydrogen bonding interaction. It was also found that water molecules existed as aggregates. With increasing water content, the water molecules occupied the adsorption sites and adsorption space of the methane, leading to a reduction in the methane excess adsorption capacity. The excess adsorption capacity of gas on chlorite decreased in the following order： carbon dioxide 〉 methane 〉 nitrogen. If the mole fraction of nitrogen or carbon dioxide in the binary gas mixture increased, the mole fraction of methane decreased, methane adsorption sites changed, and methane adsorption space was reduced, resulting in the decrease in the methane excess adsorption capacity.

关键词： Chlorite Methane Nanopores Grandcanonical Monte Carlo Adsorption capacity

Polypropylene crisper and 1-MCP delay the softening,lignification and transcription levels of related enzyme genes of golden needle mushrooms(Flammulina velutipes)

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Journal of Integrative Agriculture 2022年第1期21卷 249-260页

作者： WANG Wen-jun li Yao li Fu-hua zeng Kai-fang MING Jian College of Food Science Southwest UniversityChongqing 400715P.R.China Research Center of Food Storage&Logistics Southwest UniversityChongqing 400715P.R.China

The fresh postharvest golden needle mushroom(Flammulina velutipes) sporocarp has a high moisture content and crisp texture, but it still has high physiological activity and respiration, leading to senescence and quality *** with 1-methylcyclopropene(1-MCP) and polypropylene(PP) crispers were used to study the changes of lignification and softening of F. velutipes during storage. The main findings were as follows: the crisper packaging could effectively prolong the storage time of F. velutipes;either the 1-MCP treatment, crisper packaging or the combination of the two treatments could significantly inhibit the accumulation of lignin and the decreases in the contents of cellulose and pectin, and had certain inhibitory effects on the activities of enzymes involved in lignification and softening including phenylalanine ammonia-lyase(PAL), cinnamyl alcohol dehydrogenase(CAD), cellulase(Cx), pectin methylesterase(PME) and polygalacturonase(PG). Among them, the inhibitory effect of the crisper packaging was higher than the 1-MCP treatment, while the combination of the two treatments was the best. The results of transmission electron microscopy(TEM) and scanning electron microscopy(SEM) showed that the crisper packaging in combination with the 1-MCP treatment could effectively maintain the integrity and stability of the F. velutipes cellular structure and inhibit the emergence of plasmolysis to prevent cell membrane rupture. The transcription levels showed that the crisper packaging and the combination of the 1-MCP treatment and crisper packing could effectively affect the expression of genes for enzymes related to lignification and softening of F. velutipes. In conclusion, 1-MCP and PP crispers could delay the lignification and softening of F. velutipes during storage.

关键词： Flammulina velutipes polypropylene crisper 1-MCP softening and lignification transcription levels

Influence of Pre-strain on the Mechanical Properties of A6111-T4P Sheet with Bake Hardening

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Acta Metallurgica Sinica(English Letters) 2015年第6期28卷 678-683页

作者： Shan-Shan li Jie-Shi Chen jun Chen Cedric Xia Danielle zeng Institute of Forming Technology and Equipment School of Materials Science and Engineering Shanghai Jiao Tong University Research and Innovation Center Ford Motor Company

A6111 is an aluminum alloy, which exhibits good formability and excellent bake hardening property. This study aimed to reveal the influence of strain path, pre-strain orientation （c0 as well as pre-strain level on the mechanical properties of A6111-T4P sheet under bake treatment through uniaxial tension test. （0-5）% pre-strain, 150-170 ℃ bake temperature and 20-30 min bake time were considered in the study by referring to the actual production process. The results show that both pre-strain level and strain path play significant roles in improving the material properties. In the condition that tensile orientation （β） parallel with pre-strain orientation （β= a）, the yield strength can be remarkably improved, and much higher parameter of n in Hockett-Sherby model can be obtained than those when tensile orientation non-parallel with pre-strain orientation （a≠ β）. In addition, when the pre-strain level, paint bake process were settled and β = a, the curves obtained in five tension orientations are similar in the plastic deformation stage.

关键词： Aluminum alloy Pre-strain Bake hardening Mechanical properties