Polypropylene crisper and 1-MCP delay the softening,lignification and transcription levels of related enzyme genes of golden needle mushrooms(Flammulina velutipes)

作     者：WANG Wen-jun LI Yao LI Fu-hua ZENG Kai-fang MING Jian WANG Wen-jun;LI Yao;LI Fu-hua;ZENG Kai-fang;MING Jian

作者机构：College of Food ScienceSouthwest UniversityChongqing 400715P.R.China Research Center of Food Storage&LogisticsSouthwest UniversityChongqing 400715P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2022年第21卷第1期

页      面：249-260页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学] 083202[工学-粮食、油脂及植物蛋白工程] 

基　　金：supported by the National Natural Science Foundation of China(31471576) the Chongqing Science and Technology Commission,China(cstc2015shmszx80019) 

主　　题：Flammulina velutipes polypropylene crisper 1-MCP softening and lignification transcription levels 

摘      要：The fresh postharvest golden needle mushroom(Flammulina velutipes) sporocarp has a high moisture content and crisp texture, but it still has high physiological activity and respiration, leading to senescence and quality *** with 1-methylcyclopropene(1-MCP) and polypropylene(PP) crispers were used to study the changes of lignification and softening of F. velutipes during storage. The main findings were as follows: the crisper packaging could effectively prolong the storage time of F. velutipes;either the 1-MCP treatment, crisper packaging or the combination of the two treatments could significantly inhibit the accumulation of lignin and the decreases in the contents of cellulose and pectin, and had certain inhibitory effects on the activities of enzymes involved in lignification and softening including phenylalanine ammonia-lyase(PAL), cinnamyl alcohol dehydrogenase(CAD), cellulase(Cx), pectin methylesterase(PME) and polygalacturonase(PG). Among them, the inhibitory effect of the crisper packaging was higher than the 1-MCP treatment, while the combination of the two treatments was the best. The results of transmission electron microscopy(TEM) and scanning electron microscopy(SEM) showed that the crisper packaging in combination with the 1-MCP treatment could effectively maintain the integrity and stability of the F. velutipes cellular structure and inhibit the emergence of plasmolysis to prevent cell membrane rupture. The transcription levels showed that the crisper packaging and the combination of the 1-MCP treatment and crisper packing could effectively affect the expression of genes for enzymes related to lignification and softening of F. velutipes. In conclusion, 1-MCP and PP crispers could delay the lignification and softening of F. velutipes during storage.