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Identification and characterization of two morphologically distinct stem cell subpopulations from human urine samples

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Science China(Life Sciences) 2020年第5期63卷 712-723页

作者： An-Jing Chen Jin-Kui Pi jun-gen hu Yi-Zhou huang Hong-Wei Gao Sheng-Fu Li Jesse Li-Ling hui-Qi Xie Laboratory of Stem Cell and Tissue Engineering State Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan University and Collaborative Innovation Center of BiotherapyChengdu 610041China Key Laboratory of Transplant Engineering and Immunology of Ministry of Health West China HospitalSichuan UniversityChengdu 610041China

Urine-derived stem cells(USCs)have shown potentials for the treatment of skeletal and urological *** on published literature and our own data,USCs consist of heterogeneous populations of *** this paper,we identify and characterize two morphologically distinct subpopulations of USCs from human urine samples,named as spindle-shaped USCs(SS-USCs)and rice-shaped USCs(RS-USCs)*** two subpopulations showed similar clone-forming efficiency,while SS-USCs featured faster proliferation,higher motility,and greater potential for osteogenic and adipogenic differentiation,RS-USCs showed greater potential for chondrogenic ***5F1 was strongly expressed in both subpopulations,but MYC was weakly *** subpopulations showed similar patterns of CD24,CD29,CD34,CD44,CD73,CD90 and CD105 expression,while a higher percentage of RS-USCs were positive for ***-USCs were positive for VIM,weakly positive for SLC12A1 and UMOD,and negative for KRT18,NPHS1,AQP1 and AQP2,indicating a renal mesenchyme origin;while RSUSCs are positive for VIM,partially positive for KRT18,NPHS1,AQP1,SLC12A1 and UMOD,and negative for AQP2,indicating a nephron tubule *** above results can facilitate understanding of the biological characteristics of subpopulations of USCs,and provide a basis for further research and applications of such cells.

关键词： characterization cell subpopulation identification tissue origin urine-derived stem cells

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Application of antibody-conjugated small intestine submucosa to capture urine-derived stem cells for bladder repair in a rabbit model

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Bioactive Materials 2022年第8期7卷 443-455页

作者： Yu-Ting Song Yan-Qing Li Mao-Xuan Tian jun-gen hu Xiu-Ru Zhang Peng-Cheng Liu Xiu-Zhen Zhang Qing-Yi Zhang Li Zhou Long-Mei Zhao Jesse Li-Ling hui-Qi Xie Laboratory of Stem Cell and Tissue Engineering Orthopedic Research InstituteMed-X Center for MaterialsState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengduSichuan610041China Department of Aesthetic Surgery The People’s Hospital of PengzhouChengduSichuan611930China Surgery of Spine and Spinal Cord Henan Provincial People’s HospitalZhengzhouHenan450000China Department of Burn and Plastic Surgery West China HospitalSichuan UniversityChengduSichuan610041China Research Core Facility of West China Hospital Sichuan UniversityChengduSichuan610041China Department of Medical genetics and Prenatal Diagnosis West China Second HospitalSichuan UniversityChengduSichuan610041China

The need for bladder reconstruction and side effects of cystoplasty have spawned the demand for the development of alternative material *** such as submucosa of small intestine(SIS)have been widely used as patches for bladder repair,but the outcomes are not fully *** capture stem cells in situ has been considered as a promising strategy to speed up the process of re-cellularization and *** this study,we have developed an anti-CD29 antibody-conjugated SIS scaffold(AC-SIS)which is capable of specifically capturing urine-derived stem cells(USCs)in situ for tissue repair and *** scaffold has exhibited effective capture capacity and sound *** vivo experiment proved that the AC-SIS scaffold could promote rapid endothelium healing and smooth muscle *** endogenous stem cell capturing scaffolds has thereby provided a new revenue for developing effective and safer bladder patches.

关键词： Regenerative medicine Bladder tissue engineering Scaffold Antibody-conjugated

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Procyanidins-crosslinked small intestine submucosa: A bladder patch promotes smooth muscle regeneration and bladder function restoration in a rabbit model

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Bioactive Materials 2021年第6期6卷 1827-1838页

作者： Xiu-Zhen Zhang Yan-Lin Jiang jun-gen hu Long-Mei Zhao Qiu-Zhu Chen Yan Liang Yi Zhang Xiong-Xin Lei Rui Wang Yi Lei Qing-Yi Zhang Jesse Li-Ling hui-Qi Xie Laboratory of Stem Cell and Tissue Engineering Orthopedic Research InstituteState Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan University and Collaborative Innovation Center of BiotherapyChengduSichuanChina Research Core Facility of West China Hospital Sichuan UniversityChengduSichuanChina

Currently the standard surgical treatment for bladder defects is augmentation cystoplasty with autologous tissues,which has many side *** such as small intestine submucosa(SIS)can provide an alternative scaffold for the repair as bladder *** studies have shown that SIS could enhance the capacity and compliance of the bladder,but its application is hindered by issues like limited smooth muscle regeneration and stone formation since the fast degradation and poor mechanical properties of the ***(PC),a natural bio-crosslinking agent,has shown anti-calcification,anti-inflammatory and anti-oxidation *** importantly,PC and SIS can crosslink through hydrogen bonds,which may endow the material with enhanced mechanical property and stabilized *** this study,various concentrations of PC-crosslinked SIS(PC-SIS)were prepared to repair the full-thickness bladder defects,with an aim to reduce complications and enhance bladder *** vitro assays showed that the crosslinking has conferred the biomaterial with superior mechanical property and anti-calcification property,ability to promote smooth muscle cell adhesion and upregulate functional genes *** a rabbit model with bladder defects,we demonstrated that the PC-SIS scaffold can rapidly promote in situ tissue regrowth and regeneration,in particular smooth muscle remodeling and improvement of urinary *** PC-SIS scaffold has therefore provided a promising material for the reconstruction of a functional bladder.

关键词： Bladder reconstruction Small intestinal submucosa Procyanidins Bio-crosslinking Smooth muscle regeneration

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粉末冶金法生产深冲钽片的实验研究

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稀有金属与硬质合金 2017年第4期45卷 67-70页

作者：刘俊荣胡根火朱锋李奇超九江有色金属冶炼股份有限公司江西九江332014

采用粉末冶金法制备纯Ta锭坯,坯料经锻造、轧制成厚度为0.28mm的Ta片。研究了不同的粉末粒度配比、中间退火温度、成品退火温度对Ta片物理性能的影响。结果表明,粉末冶金法制备的纯Ta片的物理性能如抗拉强度、延伸率、显微硬度是可以通... 详细信息

采用粉末冶金法制备纯Ta锭坯,坯料经锻造、轧制成厚度为0.28mm的Ta片。研究了不同的粉末粒度配比、中间退火温度、成品退火温度对Ta片物理性能的影响。结果表明,粉末冶金法制备的纯Ta片的物理性能如抗拉强度、延伸率、显微硬度是可以通过不同的粉末粒度配比、中间退火温度及成品退火温度进行一定范围的调整,生产出的Ta片性能达到客户要求。

关键词：钽片 Ta 粉末冶金粒度配比退火温度物理性能

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模态综合超单元法及其在船舶动态计算中的应用

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上海力学 1982年第4期 8-18页

作者：恽伟君段根宝胡仲根

根据动力变换原理和超单元的模态分析,本文导出一种新的动态子结构法—模态综合超单元法。用这一方法预测了具有1224个自由度的5000吨大舱口驳船理想模型的主要模态特性,所得数值结果与模态识别测试及Moire干涉横向测振数据比较,其吻合... 详细信息

根据动力变换原理和超单元的模态分析,本文导出一种新的动态子结构法—模态综合超单元法。用这一方法预测了具有1224个自由度的5000吨大舱口驳船理想模型的主要模态特性,所得数值结果与模态识别测试及Moire干涉横向测振数据比较,其吻合程度是令人满意的。

关键词：超单元模态分析变换原理船舶

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POEM与LHM在贲门失弛缓症治疗有效性和安全性的Meta分析

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世界华人消化杂志 2017年第9期25卷 792-802页

作者：胡小军王根旺张龙龙钱彬彬顾越雷李峰杨孙虎南方医科大学第五附属医院肝胆外科广东省广州市510900 上海交通大学医学院附属第九人民医院上海市201999

目的比较经口内镜下食管肌层切开术(per-oral endoscopic myotomy,POEM)与腹腔镜Heller肌切开术(laparoscopic Heller's myotomy,LHM)临床有效性和安全性,为贲门失弛缓症最佳治疗提供证据.方法检索PubMed、EMBASE、the Cochrane Library、SCI数据库、CNKI和维普中文科技期刊数据库,检索语言为中英文,检索时间为各数据库建库时间至2016-02-20截止.采用Review Manager version5.3软件进行数据分析.各研究间的异质性通过Q检验和I2来检验.结果总共有8篇非随机对照研究纳入统计分析,共574例患者,其中POEM组245例,LHM组329例.Meta分析结果显示:(1)临床有效率:相比LHM组,POEM组有更高的临床有效率(RR=1.14,95%CI:1.02-1.27,P=0.02),异质性检验P=0.60,I^2=0%;(2)主要并发症:POEM组和LHM组,两者在主要并发症上没有统计学差异(RR=1.16,95%CI:0.76-1.78,P=0.49),异质性检验P=0.43,I^2=0%;(3)全部并发症:两组间全部并发症没有显著的统计学差异(RR=0.99,95%CI:0.72-1.36,P=0.94],异质性检验P=0.12,I^2=39%;(4)住院时间:两组间住院时间没有统计学意义(MD=-0.46,95%CI:-1.09-0.16,P=0.14),异质性检验P=0.0007,I^2=76%;(5)手术时间:两组间手术时间没有统计学意义(MD=-35.45,95%CI:-87.01-16.10,P=0.18),异质性检验:P<0.00001,I^2=98%.结论 POEM优于LHM,但本文还无法明确POEM是否是贲门失弛缓症的最佳治疗方法 ,需要进一步验证.

关键词：贲门失弛缓症经口内镜下食管肌层切开术腹腔镜Heller肌切开术 Meta分析

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Photo‑Energized MoS_(2)/CNT Cathode for High‑Performance Li–CO_(2)Batteries in a Wide‑Temperature Range

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Nano-Micro Letters 2025年第1期17卷 160-175页

作者： Tingsong hu Wenyi Lian Kang hu Qiuju Li Xueliang Cui Tengyu Yao Laifa Shen Jiangsu Key Laboratory of Materials and Technologies for Energy Storage College of Materials Science and TechnologyNanjing University of Aeronautics and AstronauticsNanjing 210016People’s Republic of China

Li–CO_(2) batteries are considered promising energy storage systems in extreme environments such as Mars;however,severe performance degradation will occur at a subzero temperature owning to the sluggish reaction ***,a photo-energized strategy adopting sustainable solar energy in wide working temperature range Li–CO_(2) battery was achieved with a binder-free MoS_(2)/carbon nanotube(CNT)photo-electrode as *** unique layered structure and excellent photoelectric properties of MoS_(2) facilitate the abundant generation and rapid transfer of photo-excited carriers,which accelerate the CO_(2) reduction and Li_(2)CO_(3) decomposition upon *** illuminated battery at room temperature exhibited high discharge voltage of 2.95 V and mitigated charge voltage of 3.27 V,attaining superior energy efficiency of 90.2%and excellent cycling stability of over 120 *** at an extremely low temperature of−30℃,the battery with same electrolyte can still deliver a small polarization of 0.45 V by the photoelectric and photothermal synergistic mechanism of MoS_(2)/CNT *** work demonstrates the promising potential of the photo-energized wide working temperature range Li–CO_(2) battery in addressing the obstacle of charge overpotential and energy efficiency.

关键词： Li-CO_(2)batteries Photo-energized Wide operation-temperature Kinetics MoS_(2)

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Recovery of waste rare earth fluorescent powders by two steps acid leaching

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Rare Metals 2013年第6期32卷 609-615页

作者： Shen-gen Zhang Min Yang hu Liu De-An Pan Jian-jun Tian Institute of Advanced Materials and Technology School of Materials Science and Engineering University of Science and Technology Beijing

The effects of the acid leaching and alkali fusion on the leaching efficiency of Y,Eu,Ce,and Tb from the waste rare earth fluorescent powders were investigated in this *** results show that hydrochloric acid is better than sulfuric acid in the first acid leaching,and NaOH is better than Na2CO3in the alkali *** the first acid leaching,the Wloss is 20.94%when the waste rare earth fluorescent powders are acid leached in H?concentration 3 mol L-1and S/L ratio 1:3 for 4 h due to red powders *** better results of the alkali fusion can be got at 800℃ for 2 h when the NaOH is *** blue powders and the green powders can be dissolved into NaAlO2and oxides such as rare earth oxide(REO).The REO can be dissolved in H?concentration 5 mol L-1,S/L1:10 for 3 h in the second acid *** leaching rates of the Y,Eu,Ce,and Tb are 99.06%,97.38%,98.22%,and 98.15%,*** leaching rate of the total rare earth is 98.60%.

关键词： Waste rare earth fluorescent powders Two steps acid leaching Alkali fusion

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An Implicit Iteration Process with Errors for a Finite Family of r-strictly Asymptotically Pseudocontractive Mappings

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Acta Mathematicae Applicatae Sinica 2007年第2期23卷 281-288页

作者： Liang-gen hu Department of Mathematics Ningbo University Ningbo 315211 China

In this paper, we will establish several strong convergence theorems for the approximation of common fixed points of r-strictly asymptotically pseudocontractive mappings in uniformly convex Banach spaces using the modiied implicit iteration sequence with errors, and prove the necessary and sufficient conditions for the convergence of the sequence. Our results generalize, extend and improve the recent work, in this topic.

关键词： Implicit iteration process with errors uniformly convex strictly asymptotically pseudocontractive mappings common fixed point

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Is it possible to foot reflexology for infants with sensorineural hearing loss?

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World Journal of Clinical Cases 2025年第2期13卷 1-4页

作者： Min Ai Hang-Hang Zhang jun-Bang Feng Chuan-Ming Li Wen-Jing Wang Meng-Die hu Department of Anesthesiology Nanan District People's Hospital of ChongqingChongqing 400060China Department of Breast and Thyroid Surgery Chongqing Bishan District Maternal and Child Health Care HospitalChongqing 402760China Department of Medical Imaging Chongqing University Central HospitalChongqing 400014China

Foot reflexology is a non-invasive and safe complementary therapy that works by massaging the reflex zones of the feet and exerts systemic or whole-body regulation through meridian nerve *** therapy is commonly used in the treatment of various conditions such as autism and Parkinson's ***,there is limited reporting on the use of foot reflexology therapy for infants with sensorineural hearing loss(SNHL).Currently,there is no definitive conclusion on how foot reflexology therapy can influence *** editorial holds some guiding significance regarding this clinical *** aim is to present physiological evidence of how foot reflexology therapy can impact infants with SNHL,thereby enhancing clinician’s awareness of foot reflexology in treating infants with SNHL.

关键词： Foot reflexology Sensorineural Hearing Loss Future perspectives Limitations Neural conduction

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