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Role of deubiquitinase JOSD2 in the pathogenesis of esophageal squamous cell carcinoma

World Journal of Gastroenterology 2024年第6期30卷 565-578页

作者： Wen-Peng Wang Dan Shi Duo Yun jun Hu Jie-Fu Wang Jia Liu Yan-Peng Yang Ming-Rui Li jun-fengwang Da-Lu Kong Department of Colorectal Oncology Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerTianjin’s Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin 300060China Department of Gastrointestinal Surgery Tianjin Nan Kai HospitalTianjin Medical UniversityTianjin Key Laboratory of Acute Abdomen Disease Associated Organ Injury and ITCWM RepairInstitute of Integrative Medicine for Acute Abdominal DiseasesTianjin 300100China Department of Oncology The First Hospital of HohhotHohhot 010000Inner Mongolia Autonomous RegionChina Department of Endocrinology Dazhou Central HospitalDazhou 635000Sichuan ProvinceChina

BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a deadly malignancy with limited treatment ***(DUBs)have been confirmed to play a crucial role in the development of malignant ***2 is a DUB involved in con-trolling protein deubiquitination and influencing critical cellular processes in *** To investigate the impact of JOSD2 on the progression of *** Bioinformatic analyses were employed to explore the expression,prognosis,and enriched pathways associated with JOSD2 in *** transduction was utilized to manipulate JOSD2 expression in ESCC cell lines(KYSE30 and RESULTS )Preliminary research indicated that JOSD2 was highly expressed in ESCC tissues,which was associated with poor *** analysis demonstrated that JOSD2 was upregulated in ESCC cell lines compared to normal esophageal ***2 knockdown inhibited ESCC cell activity,including proliferation and colony-forming ***,JOSD2 knockdown decreased the drug resistance and migration of ESCC cells,while JOSD2 overexpression enhanced these *** vivo xenograft assays further confirmed that JOSD2 promoted tumor proliferation and drug resistance in ***,JOSD2 appears to activate the MAPK/ERK and PI3K/AKT signaling *** spectrometry was used to identify crucial substrate proteins that interact with JOSD2,which identified the four primary proteins that bind to JOSD2,namely USP47,IGKV2D-29,HSP90AB1,and *** JOSD2 plays a crucial role in enhancing the proliferation,migration,and drug resistance of ESCC,suggesting that JOSD2 is a potential therapeutic target in ESCC.

关键词： Esophageal squamous cell carcinoma JOSD2 Ubiquitination Biomarker Targeted therapy Drug resistance

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The mitigation effects of exogenous dopamine on low nitrogen stress in Malus hupehensis

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Journal of Integrative Agriculture 2020年第11期19卷 2709-2724页

作者： LIU Xiao-min GAO Teng-teng ZHANG Zhi-jun TAN Ke-xin JIN Yi-bo ZHAO Yong-juan MA fengwang LI Chao State Key Laboratory of Crop Stress Biology for Arid Areas Ministry of Science and Technology/Shaanxi Key Laboratory of AppleNorthwest A&F UniversityYangling 712100P.R.China

Dopamine plays numerous physiological roles in *** explored its role in the regulation of growth,nutrient absorption,and response to nitrogen(N)deficiency in Malus hupehensis *** low N condition,plant growth slowed,and the net photosynthetic rates,chlorophyll contents,and maximal quantum yield of PSII(Fv/Fm)decreased ***,the application of 100μmol L−1 exogenous dopamine significantly reduced the inhibition of low N stress on plant *** addition to modifying root system architecture under low N supply,exogenous dopamine also changed the uptake,transport,and distribution of N,P,and ***,exogenous dopamine enhances the tolerance to low nitrogen stress by increasing the activity of enzymes(nitrate reductase,nitrite reductase,glutamic acid synthase and glutamine synthetase)involved in N *** also found that exogenous dopamine promoted the expression of ethylene signaling genes(ERF1,ERF2,EIL1,ERS2,ETR1,and EIN4)under low N ***,we hypothesized that ethylene might be involved in dopamine response to low N stress in *** results suggest that exogenous dopamine can mitigate low N stress by regulating the absorption of mineral nutrients,possibly through the regulation of the ethylene signaling pathway.

关键词： nitrogen deficiency dopamine root system architecture ethylene Malus hupehensis

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Genome-wide identification of droughtresponsive microRNAs in two sets of Malus from interspecific hybrid progenies

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Horticulture Research 2019年第1期6卷 794-811页

作者： Chundong Niu Haiyan Li Lijuan Jiang Mingjia Yan Cuiying Li Dali Geng Yinpeng Xie Yan Yan Xiaoxia Shen Pengxiang Chen jun Dong fengwang Ma Qingmei Guan State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple College of HorticultureNorthwest A&F UniversityYanglingShaanxi 712100China

Drought stress can negatively impact apple fruit quality and *** microRNAs(miRNAs)participate in apple tree and fruit development,as well as in biotic stress tolerance;however,it is largely unknown whether these molecules are involved in the drought *** identify drought-responsive miRNAs in Malus,we first examined the drought stress tolerance of ten F1 progenies of R3(M.×domestica)×*** performed Illumina sequencing on pooled total RNA from both drought-tolerant and drought-sensitive *** sequencing results identified a total of 206 known miRNAs and 253 candidate novel miRNAs from drought-tolerant plants and drought-sensitive plants under control or drought *** identified 67 miRNAs that were differentially expressed in drought-tolerant plants compared with drought-sensitive plants under drought *** drought stress,61 and 35 miRNAs were differentially expressed in drought-tolerant and drought-sensitive plants,*** determined the expression levels of seven out of eight miRNAs by stem-loop qPCR *** also predicted the target genes of all differentially expressed miRNAs and identified the expression of some *** Ontology analyses indicated that the target genes were mainly involved in stimulus response and cellular and metabolic ***,we confirmed roles of two miRNAs in apple response to *** results reveal candidate miRNAs and their associated mRNAs that could be targeted for improving drought tolerance in Malus species,thus providing a foundation for understanding the molecular networks involved in the response of apple trees to drought stress.

关键词： drought mannitol Malus

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Genome-wide identification,molecular evolution, and expression divergence of the hexokinase gene family in apple

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Journal of Integrative Agriculture 2021年第8期20卷 2112-2125页

作者： ZHU Ling-cheng SU Jing JIN Yu-ru ZHAO Hai-yan TIAN Xiao-cheng ZHANG Chen MA fengwang LI Ming-jun MA Bai-quan State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple College of HorticultureNorthwest A&F UniversityYangling 712100P.R.China Department of Plant Biology University of Illinois at Urbana-ChampaignUrbanaIL 61801USA

Hexokinase(HXK)is the first irreversible catalytic enzyme in the glycolytic pathway,which not only provides energy for plant growth and development but also serves as a signaling molecule in response to environmental ***,the evolutionary pattern of the HXK gene family in apple remains *** this study,a total of nine HXK genes were identified in the Malus×domestica genome GDDH13 *** physiological and biochemical properties,exonintron structures,conserved motifs,and cis-elements of the MdHXK genes were *** subcellular localization indicated that the MdHXK genes were mainly distributed in the mitochondria,cytoplasm,and *** duplication revealed that whole-genome duplication(WGD)and segmental duplication played vital roles in MdHXK gene family ***ωvalues of pairwise MdHXK genes indicated that this family was subjected to strong purifying selection during apple ***,five subfamilies were classified,and recent/old duplication events were identified based on phylogenetic tree *** evolutionary rates were estimated among the various HXK ***,divergent expression patterns of the MdHXK genes in four source-sink tissues and at five different apple fruit developmental stages indicated that they play vital roles in apple fruit development and sugar *** study provides a theoretical basis for future elucidation of the biological functions of the MdHXK genes during apple fruit development.

关键词： apple hexokinase cis-element screening evolutionary pattern sugar accumulation

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基于海藻酸钠电沉积技术制备ZnO量子点及其复合膜的检测应用研究

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高分子学报 2022年第2期53卷 145-152页

作者：刘俊夏传杰王康龙李端明王清华周俊杰陶青王艺峰武汉理工大学材料科学与工程学院武汉430070

天然高分子电沉积技术为构建新型功能材料和器件提供了契机.ZnO量子点(QDs)具备无毒、荧光稳定等特点日益受到人们的重视.本工作基于天然高分子海藻酸钠的配位电沉积技术制备ZnO QDs及其纳米复合膜;所采用方法具备操作简单、易于控制、... 详细信息

天然高分子电沉积技术为构建新型功能材料和器件提供了契机.ZnO量子点(QDs)具备无毒、荧光稳定等特点日益受到人们的重视.本工作基于天然高分子海藻酸钠的配位电沉积技术制备ZnO QDs及其纳米复合膜;所采用方法具备操作简单、易于控制、条件温和、绿色环保等优点;并且产物后处理简便,可以直接在电极上构建出ZnO QDs/海藻酸钠复合膜.测试结果表明复合膜中存在大小均一的ZnO QDs,平均粒径为6 nm.复合膜的外观均一平整,呈现明显的橙色荧光.ZnO QDs/海藻酸钠复合膜不仅对K3[Fe(CN)6]具有电化学检测作用,而且对Cu2+具有荧光检测作用.本工作为ZnO QDs的制备以及QDs/天然高分子纳米复合材料的构建提供一种新方法,所制得的复合膜在电化学检测和荧光检测领域具有应用价值.

关键词：电沉积海藻酸钠 ZnO量子点纳米复合膜检测

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G.41×新疆野苹果树体生长性状的QTL精细定位及候选基因预测

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中国农业科学 2018年第11期51卷 2155-2164页

作者：董军王京梁微马百全董丽娟马锋旺符轩畅李翠英西北农林科技大学园艺学院/旱区作物逆境生物学国家重点实验室

【目的】矮化砧木可以诱导地上部接穗品种矮化,实现苹果的丰产和稳产。因此,深入挖掘调控苹果树体生长的基因,可以改善苹果树形,加强苹果园地的管理方便性。【方法】以矮化苹果砧木‘G.41’和乔化苹果砧木新疆野苹果(Malus sieversii)... 详细信息

【目的】矮化砧木可以诱导地上部接穗品种矮化,实现苹果的丰产和稳产。因此,深入挖掘调控苹果树体生长的基因,可以改善苹果树形,加强苹果园地的管理方便性。【方法】以矮化苹果砧木‘G.41’和乔化苹果砧木新疆野苹果(Malus sieversii)为亲本构建的188株F1代分离群体为试材,并于每个分离群体植株上嫁接‘富士冠军’。基于SSR标记技术,采用Join Map 4.0作图软件构建苹果遗传连锁图,应用Map QTL 5.0作图软件,结合后代群体的表型数据,对接穗高度和接穗横截面积生长性状进行初步QTL定位。结合苹果基因组序列信息,利用Primer5.0软件进行新SSR标记开发,并对初步定位区域进行精细定位及候选基因预测。【结果】该研究从361对SSR引物中筛选出108对在亲本之间表现出多态的SSR引物,多态率为29.9%。其中95对引物用于遗传连锁图谱构建,并在5号连锁群上初步检测出4个与植株生长性状相关的QTL位点,与接穗高度、接穗横截面积性状紧密连锁的两个标记,为L05024和Hi09b04。根据初步定位区域的序列信息,设计了新的SSR引物24对,其中在亲本间表现出多态的有10对,利用该10对引物对5号连锁群重新分析。构建了包含21个SSR标记、总长为86.0 c M的高密度遗传图谱,其平均遗传距离为7.52 c M。通过对接穗高度与接穗横截面积生长相关性状的QTL分析,在SSR标记L05024和Hi09b04之间找到与其相关的QTL位点,其表型贡献率分别为19.2%和51.7%。将该QTL位点与基因组序列对比,发现其物理距离为543 kb,并且该QTL区间包含16个候选基因。推测其中MDP0000323212为与植株生长密切相关的基因。【结论】本研究将砧木诱导矮化基因定位于苹果第5号染色体543 kb区段内,侧翼标记分别为L05024和Hi09b04,其物理距离为4.048—4.591 Mb,并筛选到可能参与调控植株生长的候选基因MDP0000323212。

关键词：苹果砧木 SSR 遗传图谱 QTL 候选基因

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介质折射率对对称结构光子晶体内部局域电场的调制

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河池学院学报 2016年第5期36卷 54-58页

作者：唐秀福罗家昌卢逢旺刘俊河池学院物理与机电工程学院广西宜州546300

利用传输矩阵法理论,研究介质折射率对镜像对称结构一维光子晶体内部局域电场的调制,揭示介质折射率的变化与内部局域电场强度变化之间的规律,结果表明:对于对称结构光子晶体(AB)5(BA)5,随着介质A的折射率或A、B介质的折射率比值的增大... 详细信息

利用传输矩阵法理论,研究介质折射率对镜像对称结构一维光子晶体内部局域电场的调制,揭示介质折射率的变化与内部局域电场强度变化之间的规律,结果表明:对于对称结构光子晶体(AB)5(BA)5,随着介质A的折射率或A、B介质的折射率比值的增大,内部局域电场降低;随着介质B的折射率增大,内部局域电场增强。对于光子晶体(AB)5(BA)2(AB)2(BA)5,随着介质A、B的折射率之差的绝对值增大,光子晶体内部局域电场增强,对光在光子晶体中传播的局域限制作用增大,光子晶体的分立透射峰也越来越精细。

关键词：光子晶体折射率局域电场调制对称结构

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超导热管技术在低温烟气余热回收中的应用

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能源与节能 2023年第5期 84-89页

作者：郑峰高志新李小刚杜骏魏凤旺张俊克郭晓龙河北建投任丘热电有限责任公司河北任丘062550

为了解决锅炉排烟温度过高的问题,进行烟气回收利用是提高机组利用率的重要措施。具体的方法包括增加换热面积、运用烟气冷却器、加装低温省煤器。通过比较发现,加装低温省煤器是一种节能且经济的余热利用方式,采用热管式低温省煤器的... 详细信息

为了解决锅炉排烟温度过高的问题,进行烟气回收利用是提高机组利用率的重要措施。具体的方法包括增加换热面积、运用烟气冷却器、加装低温省煤器。通过比较发现,加装低温省煤器是一种节能且经济的余热利用方式,采用热管式低温省煤器的方式最有益且最普遍。利用热管式低温省煤器能够解决锅炉排烟温度高而造成能量大量浪费的问题,回收烟温效果明显,利用效率高,有很大的发展前景。

关键词：烟气冷却器低温省煤器热管

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