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Protective Effects of Anthocyanins Extracted from Vaccinium Uliginosum on 661W Cells Against Microwave-Induced Retinal Damage

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Chinese Journal of Integrative Medicine 2022年第7期28卷 620-626页

作者： YIN Lan FAN si-jun ZHANG Mao-nian Department of Ophthalmology Chinese PLA General HospitalBeijing 100853China Department of Ophthalmology PLA Rocket Characteristic Medical CenterBeijing 100853China

Objective: To study the protective effect of anthocyanins extracted from Vaccinium Uliginosum(VU)on retinal 661W cells against microwave radiation induced retinal injury. Methods: 661W cells were divided into 6 groups, including control, model [661W cells radiated by microwave(30 mW/cm2, 1 h)] and VU groups [661W cells pretreated with anthocyanins extracted from VU(25, 50, 100 and 200 μg/mL, respectively) for 48 h, and radiated by microwave 30 mW/cm2, 1 h]. After treatment with different interventions, the cell apoptosis index(AI)was determined using Heochst staining;contents of malonaldehyde(MDA), glutataione(GSH), and activity of superoxide dismutase(SOD) were measured. mRNA expressions of nuclear factor erythroid 2-related factor 2(Nrf2) and heme oxygenase 1(HO-1) were detected by real time quantitative polymerase chain reaction, and the expression of HO-1 protein was examined by Western blot analysis. Nucleus and cytoplasm were separated and Nrf2 protein expression was further verified by Western blot analysis. Results: There was significant difference in AI among the groups(F=322.83, Psignificantly higher in the model group and was lower in 4 VU-pretreated groups(Psion analysis showed the decline of AI was in a dose-dependent manner with VU treatment(r=0.8419, Psignificantly lower than the model group(Psignificantly higher(all Psions were slightly increased after irradiation, and obviously increased in 100 μg/mL VU-treated group. After irradiation, the relative expressions of HO-1 and Nrf2 proteins in nucleus were slightly increased(Psignificantly increased in both nucleus and cytoplasm in the VU treatment group

关键词： Vaccinium Uliginosum anthocyaninsm photoreceptor microwave apoptosis nuclear factor erythroid 2-related factor 2/heme oxygenase 1

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Enhanced alumina film adhesion of Hf/Y-doped iron-aluminum alloys during high-temperature oxidation:a new observation

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Rare Metals 2019年第9期38卷 877-884页

作者： Dong-Qing Li Li-Xian Zhou jun Zhan Jing-Chao Wang Jian Gu Jia-jun si Department of Transmission and Transformation Engineering Mechanics China Electric Power Research Institute

The cyclic oxidation behavior of Hf/Y-doped B2 FeAl intermetallics at 1373 K was *** an undoped FeAl alloy,premature spallation of the alumina film occurs due to the formation of numerous voids at the film/alloy interface and apparent shrinkage in the *** contrast to this,doping with either Hf or Y significantly improves the interfacial adhesion between the alumina film and the alloy substrate,particularly with *** observation in combination with Anger electron spectroscopic analysis suggests that in addition to prohibiting interfacial void formation and alleviating film shrinkage,the addition of Hf in the FeAl alloy could consolidate the film/alloy interface by directly participating in chemical bonding across the interface as a Hf *** causes the spallation of alumina film from the equiaxed grains/columnar grains interface rather than the bottom of the film.

关键词： Intermetallics Reactive elements Thermal cycling Alumina film Interfacial adhesion Segregation

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Electrocatalytic activity of non-precious metal catalyst Co-N/C toward oxygen reduction reaction

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Chinese Chemical Letters 2010年第8期21卷 983-986页

作者： Yu jun si Chang Guo Chen Wei Yin Hui Cai College of Chemistry and Chemical Engineering Chongqing University Chongqing 400044 China College of Chemistry and Pharmaceutical Engineering Sichuan University of Science and Engineering Zigong 643000 China The Key Laboratory for the Exploitation of Southwest Resources and Environmental Disaster Control Engineering under Ministry of Education Chongqing University Chongqing 400044 China

Metallic cobalt was deposited on acetylene black to synthesize a composite Co/C by chemical reduction method.A platinumfree electrocatalyst Co-N/C（800） for oxygen reduction reaction（ORR） was synthesized by mixing the composite Co/C with urea and heat-treating at 800℃.The results from linear sweep voltammograms indicated that the Co-N/C（800） is active to ***β-Co and cobalt oxides are not the active site of the catalyst Co-N/***,the existence of cobalt facilitated the modification of nitrogen to carbon black and led to the formation of active site of catalyst Co-N/C（800）.

关键词： Oxygen reduction reaction Electrocatalyst Non-precious metal Active site

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silylamido supported dinitrogen heterobimetallic complexes: syntheses and their catalytic ability

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National Science Review 2021年第12期8卷 29-36页

作者： Dan-dan Zhai si-jun Xie Yi Xia Hua-yi Fang Zhang-jie Shi Department of Chemistry Fudan University State Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic ChemistryChinese Academy of Sciences

Molybdenum dinitrogen complexes supported by monodentate arylsilylamido ligand,[Ar(Mesi)N]MoNMg(THF)[N(siMe)Ar](5) and [Ar(Mesi)N]MoNsiMe(6)(Ar=3,5-MeCH) were synthesized and structurally characterized,and proved to be effective catalysts for the disproportionation of cyclohexadienes and isomerization of terminal ***~1 H NMR spectrum suggested that the bridging nitrogen ligand remains intact during the catalytic reaction,indicating possible catalytic ability of the Mo-N=N motif.

关键词： dinitrogen fixation dinitrogen-metal complex catalytic ability disproportionation isomerization

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A mixed-valent high spin(μ-hydroxo)dicobalt(Ⅱ/Ⅲ) complex and its end-on type dioxygen adduct: synthesis, geometric and electronic structure studies

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Science China Chemistry 2021年第10期64卷 1693-1697页

作者： Ceng-Ceng Du si-jun Xie Dan-Dan Zhai Zhang-Jie Shi Huayi Fang Department of Chemistry Fudan UniversityShanghai 200433China School of Materials Science and Engineering Tianjin Key Lab for Rare Earth Materials and ApplicationsNankai UniversityTianjin 300350China

A mixed-valent high spin (μ-hydroxo)dicobalt(Ⅱ/Ⅲ) complex bearing N,N′-bis(5-tertbutylsalicylidene)-1,2-diaminobenzene(Salophen^(tBu)) ligand was synthesized by reacting (Salophen^(tBu))Co(Ⅱ)(Py) with O_(2) at low *** complex was isolated and structurally characterized by single crystal X-ray diffraction and infrared *** measurement showed a quartet ground state for this complex at room temperature,which was also supported by electron paramagnetic resonance (EPR)*** investigations indicated the deviation of the equatorial N_(2)O_(2) coordination geometry around the Co(Ⅱ) center from plane stabilized the quartet *** end-on type O_(2) adduct of this (μ-hydroxo)dicobalt(Ⅱ/Ⅲ) complex was isolated as dark-brown crystals and structurally and spectroscopically *** measurement of this compound in solid state at room temperature presented an isotropic singlet at g~2,revealing the superoxide-like electronic feature of the coordinated O_(2) and was in line with the calculation *** reactivity of the Co(Ⅲ)-O_(2) complex with N-hydroxyphthalimide,which can act as hydrogen atom donor,was also examined.

关键词： dicobalt complex mixed-valent electronic structure dioxygen adduct

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Efficient BoPDS Gene Editing in Cabbage by the CRISPR/Cas9 System

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Horticultural Plant Journal 2019年第4期5卷 164-169页

作者： Cunfa Ma Mengci Liu Qinfei Li jun si Xuesong Ren Hongyuan Song Key Laboratory of Horticulture Science for Southern Mountains Regions Ministry of EducationCollege of Horticulture and Landscape ArchitectureSouthwest University

Genome editing offers great advantages in identifying gene function and generating agronomically important mutations in crops. Here,we report that the genome of cabbage(Brassica oleracea var. capitata), an important cruciferous vegetable, can also be precisely edited by a CRISPR/Cas9 system stacked with multiple single-guide RNA-expressing cassettes. When the phytoene desaturase Bo PDS gene was used as the target gene, an albino-phenotype transgenic shoot in T0 Basta-resistant lines was observed, and 37.5% of the transgenic cabbage shoots carried Bo PDS gene mutations as a result of nucleotide deletions at the expected position. Moreover, mutations were detected in sites with the same target sequence in gene Bol016089 which is paralogous to the Bo PDS gene. Our results show that the CRISPR/Cas9 system is a powerful tool for cabbage variety improvement by genome editing.

关键词： Cabbage CRISPR/Cas9 BoPDS Genome editing Mutation

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Statistics of oxidation resistance of Ni-(0-15)Co-(8-15)Cr-(0-5)Mo-(0-10)W-(3-8)Al-(0-5)Ti-(0-10)Ta-0.1C-0.01B superalloys at 1000℃ by compositional variations

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Rare Metals 2020年第8期39卷 918-927页

作者： si-jun Park Kyu-Hyuk Lee Seong-Moon Seo Hi-Won Jeong Young-Soo Yoo HeeJin Jang Multi-Material Research Center Gwangju-Jeonnam DivisionKorea Automotive Technology InstituteGwangju 62207Republic of Korea Department of Advanced Materials Engineering Chosun UniversityGwangju 61452Republic of Korea High Temperature Materials Group Korea Institute of Materials ScienceChangwon 51508Republic of Korea Department of Materials Science and Engineering Chosun UniversityGwangju 61452Republic of Korea

The effects of alloying elements(Co,Cr,Mo,W,Al,Ti,and Ta)on the oxidation resistance of Ni-(0-15)Co-(8-15)Cr-(0-5)Mo-(0-10)W-(3-8)Al-(0-5)Ti-(0-10)Ta-0.1 C-0.01 B alloys were *** sample compositions were designed by the Box-Behnken method of design of experiments(DOE).The alloying elements show complicated effects on the mass gain due to oxidation,depending on the alloy *** reduces the mass gain *** other elements except Al do not appear to exert a strong effect on the oxidation rate on average,but their influences are shown clearly in the alloys with a low Al ***,W,and Ta reduce the oxidation rate,while Cr,Mo,and Ti promote *** is the most effective element in reducing the oxidation rate of the alloy with a low Al *** is confirmed that a continuous Al2O3 layer is essentially required for high oxidation *** oxide scale of easily oxidized alloys has various oxides such as NiCr2O4,NiAl2O4,NiO,Cr2O3,CrTaO4,and TiO2.

关键词： High temperature oxidation Alloy composition Ni-based superalloy Oxidation resistance Design of experiments

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New Application of Stainless Steel

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Journal of Iron and Steel Research International 2006年第1期13卷 62-66页

作者： YANG Jia-long LI Ying WANG Fu ZANG Zheng-gui LI si-jun College of Materials and Metallurgy Northeastern University Shenyang 110004 Liaoning China Ironmaking Plant Wuhan Iron and Steel Group Co Wuhan 430083 Hubei China

Several rigid substrates such as stainless steel, titanium alloy, aluminum alloy, nickel foil, silicon, and sodium lime glass have been employed for manufacturing high quality TiO2 films by metal organic chemical vapor deposition （MOCVD）. The as-deposited TiO2 films have been characterized with SEM/EDX and XRD. The photocatalytic properties were investigated by decomposition of aqueous orange Ⅱ. UV VIS photospectrometer was employed to check the absorption characteristics and photocatalytic degradation activity. The results show that films synthesized on metal substrates display higher photoactivities than that on absolute substrates such as silicon and glass. It is found that solar light is an alternative to UV-light used for illumination during photodegradation of orange Ⅱ. TiO2 film on stainless steel substrate was regarded as the best one for photocatalysis.

关键词： MOCVD photodegradation orange Ⅱ metal substrate inert substrates

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Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil

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Journal of Integrative Agriculture 2014年第2期13卷 415-425页

作者： LIU Ling-zhi QIN si-jun Lü De-guo WANG Bing-ying YANG Ze-yuan Key Laboratory for Northern Fruit Cultivation and Physio-Ecology of Shenyang City/College of Horticulture Shenyang Agricultural University College of Land and Environment Shenyang Agricultural University Foreign Language Department Shenyang Agricultural University

In this study, we investigated the potential nitrification and community structure of soil-based ammonia-oxidizing bacteria （AOB） in apple orchard soil during different growth periods and explored the effects of environmental factors on nitrification activity and AOB community composition in the soil of a Hanfu apple orchard, using a culture-dependent technique and denaturing gradient gel electrophoresis （DGGE）. We observed that nitrification activity and AOB abundance were the highest in November, lower in May, and the lowest in July. The results of statistical analysis indicated that total nitrogen （N） content, NH4＋-N content, NO3-N content, and pH showed significant correlations with AOB abundance and nitrification activity in soil. The Shannon-Winner diversity, as well as species richness and evenness indices （determined by PCR-DGGE banding patterns） in soil samples were the highest in September, but the lowest in July, when compared to additional sampled dates. The DGGE fingerprints of soil-based 16S rRNA genes in November were apparently distinct from those observed in May, July, and September, possessing the lowest species richness indices and the highest dominance indices among all four growth periods. Fourteen DGGE bands were excised for sequencing. The resulting analysis indicated that all AOB communities belonged to the 13-Proteobacteria phylum, with the dominant AOB showing high similarity to the Nitrosospira genus. Therefore, soil-based environmental factors, such as pH variation and content of NHa＋-N and NO3--N, can substantially influence the abundance of AOB communities in soil, and play a critical role in soil-based nitrification kinetics.

关键词： apple orchard soil ammonia-oxidizing bacteria potential nitrification community structure PCR-dena~'ing gradientgel electrophoresis

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A non-precious metal catalyst for oxygen reduction prepared by heat-treating a mechanical mixture of carbon black,melamine and cobalt chloride

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Chinese Chemical Letters 2013年第12期24卷 1109-1111页

作者： Yu-jun si Zhong-Ping Xiong Chang-Guo Chen Ping Liu Hui-Juan Wu College of Chemistry and Pharmaceutical Engineering Sichuan University of Science and Engineering College of Chemistry and Chemical Engineering Chongqing University

A non-precious metal catalyst CoMe]C for the oxygen reduction reaction is prepared by heat-treating a mechanical mixture of carbon black, melamine and cobalt chloride at 600 under nitrogen atmosphere for 2 h. The catalytic activity of CoMe/C is characterized by the electrochemical linear sweep voltammetry technique. The onset reduction potential of the catalyst is 0.55 V （vs. SCE） at a scanning rate of 5 mV/s in 0.5 mol/L H2SO4 solution. The formation of the ORR activity sites of CoMe/C is facilitated by metallic β- cobalt.

关键词： Oxygen reduction Non-precious metal catalyst Preparation Mechanical method

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