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基于植物电信号的小麦苗期等渗干旱胁迫与盐胁迫辨识

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农业机械学报 2021年第7期52卷 231-236页

作者：李进海李洋 RICARDO ferraz de Oliveira 姚杰鹏黄岚王忠义中国农业大学信息与电气工程学院北京100083 中国农业大学现代精细农业系统集成研究教育部重点实验室北京100083 中国农业大学农业农村部农业信息获取技术重点实验室北京100083 圣保罗大学路易斯·德奎罗兹农业学院皮拉西卡巴3538709

针对早期干旱胁迫与盐胁迫在植物处于逆境时难以区分的问题,提出一种基于光诱导植物电信号的干旱胁迫与盐胁迫辨识方法。使用光/暗循环刺激获取了小麦幼苗在正常状态以及等渗干旱胁迫与盐胁迫下的植物叶表面电位,并利用一对一分类策略... 详细信息

针对早期干旱胁迫与盐胁迫在植物处于逆境时难以区分的问题,提出一种基于光诱导植物电信号的干旱胁迫与盐胁迫辨识方法。使用光/暗循环刺激获取了小麦幼苗在正常状态以及等渗干旱胁迫与盐胁迫下的植物叶表面电位,并利用一对一分类策略的支持向量机(One-versus-one support vector machine,OVOSVM)对获取的植物电信号样本进行分类。三折交叉验证结果显示:对正常状态与干旱胁迫下小麦幼苗叶表面电位进行二分类,分类准确率达到100%;对正常状态与盐胁迫下小麦幼苗叶表面电位进行二分类,准确率为94.44%;进行三分类时准确率达到96.30%;对等渗干旱胁迫与盐胁迫下获取的小麦叶表面电位进行二分类,分类准确率为100%。研究表明,植物电信号作为一种辨识逆境胁迫的方法,可对等渗条件下早期干旱胁迫与盐胁迫进行准确区分。

关键词：小麦幼苗叶表面电位光诱导植物电信号等渗干旱胁迫与盐胁迫支持向量机

What a few hairs can tell us about the resource use of giant armadillos

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Integrative Zoology 2023年第1期18卷 129-142页

作者： Marcelo MAGIOLI Nina ATTIAS Gabriel MASSOCATO Danilo KLUYBER Marcelo Zacharias MOREIRA Katia Maria Paschoaletto Micchi de Barros ferraz Adriano Garcia CHIARELLO Arnaud L.J.deSBIEZ Instituto Pró-Carnívoros AtibaiaSão PauloBrazil Centro Nacional de Pesquisa e Conservação de Mamíferos Carnívoros(CENAP) Instituto Chico Mendes de Conservação da Biodiversidade(ICMBio)AtibaiaSão PauloBrazil Instituto de Conservação de Animais Silvestres(ICAS) Campo GrandeMato Grosso do SulBrazil Instituto de Pesquisas Ecológicas Organização Não GovernamentalNazaréPaulistaSão PauloBrazil Houston Zoo HoustonTexasUSA Naples Zoo at Caribbean Gardens NaplesFloridaUSA Laboratório de Ecologia Isotópica Centro de Energia Nuclear na AgriculturaUniversidade de São PauloPiracicabaSão PauloBrazil Laboratório de Ecologia Manejo e Conservação de Fauna Silvestre(LEMaC)Departamento de Ciências FlorestaisEscola Superior de Agricultura“Luiz de Queiroz”(ESALQ)Universidade de São Paulo(USP)PiracicabaSão PauloBrazil departamento de Biologia Faculdade de FilosofiaCiências e LetrasUniversidade de São Paulo(USP)Ribeirão PretoSão PauloBrazil RZSS-Royal Zoological Society of Scotland(RZSS) MurrayfieldEdinburghUK

Understanding of resource requirements of vulnerable species is key for conservation ***,we used sta-ble carbon(δ13C)and nitrogen isotopes(δ15N)of hair from giant armadillos(Priodontes maximus)to understand individual resource *** collected hair from 19 giant armadillos analyzing 34 samples from the Brazilian Pan-tanal and compared the resource use between sexes and age *** also assessed the relationship of isotopic values with individuals’body mass and the habitat type *** observed a wide variation in resource use by giant armadillos,showing that individuals consume prey that feeds on distinct resources and occupies different habitat types,indicating that there is individual variation in foraging *** giant armadillos presented a mixed diet(C3/C4 resources,forests/open areas),but a quarter of individuals had strictly C4 diets(open areas).Males are more prone to forage on C4 resources,presenting an isotopic niche 2.6-times larger than that of *** presented diets more associated with habitats with high arboreal cover(C3 resources,forests),while adults foraged more in open areas(C4 resources).This result is mirrored by the positive relationship betweenδ13C values and body mass,suggesting that larger giant armadillos tend to feed more in open *** observed thatδ13C values decreased as individuals increased the use of habitats with high arboreal *** stress the importance of con-serving the natural mosaic in the Pantanal landscape for the species persistence,given that giant armadillos require all habitat types to fulﬁll their dietary,spatial,and developmental needs.

关键词： diet ontogenetic changes resource partitioning stable isotopes telemetry

Fast determination of Benzodiazepines in Human Urine via Liquid-Liquid Extraction with Low Temperature Partitioning and LC-HRMS

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American Journal of Analytical Chemistry 2012年第2期3卷 118-124页

作者： Elisangela Jaqueline Magalhaes Clesia Cristina Nascentes Rodinei Augusti Maria Eliana Lopes Ribeiro de queiroz julio Cesar Cardoso da Silva Robson Jose de Cassia Franco Afonso departamento de Quimica Universidade Federal de Minas GeraisBelo HorizonteBrazil departamento de Quimica Universidade Federal de VicosaVicosaBrazil departamento de Quimica Universidade Federal de Ouro PretoOuro PretoBrazil

A simple and high-throughput method to simultaneously determine selected benzodiazepines (i.e., diazepam, lorazepam, clonazepam, and bromazepam) in urine was developed and validated. The entire methodology consisted of the application of an innovative extraction/cleanup procedure, namely liquid-liquid extraction with low-temperature partitioning (LLE-LTP), and analysis by liquid chromatography combined with high-resolution mass spectrometry (LC-HRMS). The LLE-LTP procedure was optimized via factorial design and by evaluating crucial variables, specifically the freezing mode (either slow or fast), the urine/acetonitrile volume ratio, and the sample ionic strength. The benzodiazepines were quantified using matrix-matched calibration curves where the following parameters were assessed by validation protocol: in general, linearity range of 17 - 200 μg?L–1 (r > 0.9957);limits of detection lower than 5 μg?L–1;relative standard deviations (RSD) lower than 12.5%;and accuracy ranging from 72.3 to 117%. To test this procedure’s performance, the method was applied to determine the content of diazepam in actual urine samples. The validation results obtained for the method demonstrated that the present methodology could be potentially applied in proficient laboratories as a routine approach for determining benzodiazepines compounds content in urine.

关键词： Benzodiazepines Urine LC-HRMS Extraction Procedure Low-Temperature Partitioning

Effects of Biochar on the Emissions of Greenhouse Gases from Sugarcane Residues Applied to Soils

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Agricultural Sciences 2017年第9期8卷 869-886页

作者： Thalita Fernanda Abbruzzini Mariana delgado Oliveira Zenero Pedro Avelino Maia de Andrade Fernando Dini Andreote julio Campo Carlos Eduardo Pellegrino Cerri department of Soil Science “Luiz de Queiroz” College of Agriculture Piracicaba Brazil Institute of Ecology Universidad Nacional Autónoma de México México D.F. Mexico

The sugar and bioethanol industry generate large amounts of filter cake and vinasse, residues that are applied to sugarcane fields as conditioners and organic fertilizers. However, these may be significant sources of greenhouse gases emissions to the atmosphere. This study assessed the impact of sugarcane straw biochar on the emissions of CO2, CH4and N2O promoted by filter cake and vinasse applied to soil, and its effects on the chemical properties and bacterial communities of a Typic Hapludox and a Quartzipsamment. A laboratory incubation was conducted for 100 days with both soils under five treatments: vinasse and filter cake amendment (FV), plus biochar at 10 (FV + B10), 20 (FV + B20) and 50 (FV + B50) Mg·ha-1, and a control. Soil pH, available P and exchangeable base contents increased with biochar added to sandy soil. Mineral N decreased with biochar addition to both soils. The FV treatment increased CO2 emissions by 5-fold and 2.4-fold in sandy and clayey soils, respectively, compared to the control. Moreover, FV +B10 increased CO2 emissions by 4% and 6.4% in sandy and clayey soils, respectively, compared to FV. Cumulative N2O emissions in FV were 537% and 125% higher in sandy and clayey soils, respectively, compared to the control. Nevertheless, increasing biochar amendment rates reduced N2O emissions from 24% to 34% in sandy soil, and from 14% to 56% in clayey soil. CH4 emissions were negligible. The effects of filter, vinasse and biochar amendments on soil amelioration were closely related to its buffering capacity. Temporal changes on bacterial community structure were more pronounced in the sandy soil compared to clayey, and indicated that N2O emission mitigation in clayey soil was directly related to biotic mechanisms, while abiotic mechanisms caused by biochar played a more important role in mitigating N2O emissions in sandy soil.

关键词： Filter Cake Fingerprinting Nitrous Oxide Pyrolysis Carbon Soil Fertility Vinasse

A potential clinical based score in hepatitis C viruscirrhotic patients to exclude small hepatocellular carcinoma

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Hepatoma Research 2018年第4期4卷 14-24页

作者： denise Cerqueira Paranaguá-Vezozzo Celso Eduardo Lourenco Matielo Daniel ferraz de Campos Mazo Lucas Souto Nacif Mario Guimaraes Pessoa Gleicy Luz Reinoso Pereira Roque Gabriel Rezende de Lima Patricia Momoyo Yoshimura Zitelli Suzane Kioko Ono Flair JoséCarrilho Division of Gastroenterology and Hepatology Department of GastroenterologyUniversity of Sao Paulo School of MedicineSao Paulo 05403-900Brazil Sao Paulo Clínicas Liver Cancer Group Sao PauloCancer Institute of Sao PauloUniversity of Sao Paulo School of MedicineSao Paulo 01246-000Brazil Division of Gastroenterology(Gastrocentro) School of Medical SciencesUniversity of CampinasCampinas 13083-878Brazil

Aim: Hepatitis C virus (HCV) cirrhosis is an important cause of hepatocellular carcinoma (HCC). This study aimed to identify factors of HCC presence among HCV cirrhotic patients with and without small diameter HCC (≤ 3 cm). Methods: A case control transversal study between 1998 and 2003 including 93 patients: 31 with small diameter HCC and 62 without HCC. Groups were matched by age and gender. Multiple logistic regression analysis using Akaike Information Criteria to estimate the probability of HCC was performed. A model score was generated and bootstrap analysis was performed for internal validation. Results: Three significant laboratorial variables for HCC presence were found: alanine aminotransferase > 37 U/L [odds ratio (OR): 7.43 (1.61-34.19),P = 0.01], alpha-fetoprotein > 20 ng/mL [OR: 16.2 (4.17-63.01),P del score with an area under curve of 0.79 (95% CI: 0.7-0.89) was built based on these variables. The negative predictive value of those classified as at low risk of HCC was 99.1%. Conclusion: An easy and practical model score was generated. It may be an auxiliary tool for identification of HCV patients with low probability of small diameter HCC at initial evaluation composed of three serum examinations used in routine outpatient clinical practice.

关键词： Clinical score hepatocellular carcinoma hepatitis C