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Glycosylation-related gene expression in HT29-MTX-E12 cells upon infection by Helicobacter pylori

World Journal of Gastroenterology 2017年第37期23卷 6817-6832页

作者： Michael T Cairns Ananya Gupta julie a naughton Marian Kane Marguerite Clyne Lokesh Joshi Glycoscience Group National Centre for Biomedical Engineering ScienceNational University of Ireland Galway School of Natural Sciences National University of Ireland Galway Conway Institute of Biomolecular and Biomedical Research School of Medicine and Medical Sciences University College Dublin

AIM To identify glycosylation-related genes in the HT29 derivative cell line, HT29-MTX-E12, showing differential expression on infection with Helicobacter pylori(H. pylori).METHODS Polarised HT29-MTX-E12 cells were infected for 24 h with H. pylori strain 26695. After infection RNA was isolated from both infected and non-infected host cells. Sufficient infections were carried out to provide triplicate samples for microarray analysis and for q RTPCR analysis. RNA was isolated and hybridised to Affymetrix arrays. Analysis of microarray data identified genes significantly differentially expressed upon infection. Genes were grouped into gene ontology functional categories. Selected genes associated with host glycan structure(glycosyltransferases, hydrolases, lectins, mucins) were validated by real-time q RT-PCR *** Infection of host cells was confirmed by the isolation of live bacteria after 24 h incubation and by PCR amplification of bacteria-specific genes from the host cell RNA. H. pylori do not survive incubation under the adopted culture conditions unless they associate with the adherent mucus layer of the host cell. Microarray analysis identified a total of 276 genes that were significantly differentially expressed(P < 0.05) upon H. pylori infection and where the fold change in expression was greater than 2. Six of these genes are involved in glycosylation-related processes. Real-time q RT-PCR demonstrated significant downregulation(1.8-fold, P < 0.05) of the mucin MUC20. REG4 was heavily expressed and significantly downregulated(3.1-fold, P < 0.05) upon infection. Gene ontology analysis was consistent with previous studies on H. pylori *** Gene expression data suggest that infection with H. pylori causes a decrease in glycan synthesis, resulting in shorter and simpler glycan structures.

关键词： Glycosylation 支持者粘液胃 HT29-MTX-E12 H。pylori 紧张 26695 Transcriptomics

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Abomasal infusion of branched-chain amino acids or branched-chain keto-acids alter lactation performance and liver triglycerides in fresh cows

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Journal of Animal Science and Biotechnology 2024年第3期15卷 1145-1157页

作者： Kristen Gallagher Isabelle Bernstein Cynthia Collings David Main Ghayyoor Ahmad Sarah naughton Jayasimha Daddam Vengai Mavangira Mike Vandehaar Zheng Zhou Department of Animal Science Michigan State UniversityEast Lansing 48824USA Department of Veterinary Diagnostic and Production Animal Medicine Ames 50011USA

Background Dairy cows are at high risk of fatty liver disease in early lactation,but current preventative measures are not always *** with fatty liver have lower circulating branched-chain amino acid(BCAA)concentra-tions whereas cows with high circulating BCAA levels have low liver triglyceride(TG).Our objective was to determine the impact of BCAA and their corresponding ketoacids(branched-chain ketoacids,BCKA)on production performance and liver TG accumulation in Holstein cows in the first 3 weeks *** Thirty-six multiparous Holstein cows were used in a randomized block design *** were abomasally infused for the first 21 d postpartum with solutions of 1)saline(CON,n=12);2)BCA(67 g valine,50 g leu-cine,and 34 g isoleucine,n=12);and 3)BCK(77 g 2-ketovaline calcium salt,57 g 2-ketoleucine calcium salt,and 39 g 2-ketoisoleucine calcium salt,n=12).All cows received the same *** effects were determined using PROC GLIMMIX in *** No differences were detected for body weight,body condition score,or dry matter intake averaged over the first 21 d *** receiving BCK had significantly lower liver TG concentrations compared to CON(6.60%vs.4.77%,standard error of the mean(SEM)0.49)during the first 3 weeks of *** of BCA increased milk yield(39.5 vs.35.3 kg/d,SEM 1.8),milk fat yield(2.10 vs.1.69 kg/d,SEM 0.08),and lactose yield(2.11 vs.1.67 kg/d,SEM 0.07)compared with *** to CON,cows receiving BCA had lower plasma glucose(55.0 vs.59.2 mg/dL,SEM 0.86)but higherβ-hydroxybutyrate(9.17 vs.6.00 mg/dL,SEM 0.80).Conclusions Overall,BCAA supplementation in this study improved milk production,whereas BCKA supplementa-tion reduced TG accumulation in the liver of fresh cows.

关键词： Branched-chain amino acids Branched-chain ketoacids Fatty liver Fresh cow

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中国电动汽车卷向底特律美国买家间接获益

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商业周刊（中文版） 2024年第5期 26-28页

作者： Keith naughton 程玺(译) 不详

现在美国人还买不到中国品牌的电动汽车。也没有人真正知道中国产电动汽车何时能够进入美国市场。但中国低价电动汽车的发展前景已经让底特律车企寝食难安。

关键词：电动汽车发展前景底特律美国市场

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里程焦虑未解决美国人购买电动汽车意愿下降

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商业周刊（中文版） 2024年第3期 50-51页

作者： Keith naughton 融汐(译) 不详

高昂的价格、高昂的融资利率以及对续航里程的担忧,将导致2024年美国电动汽车的普及放缓。当黛比·梅滕雷特(Debbie Mettenleiter)的旧现代汽车在行驶了20万英里后需要更换时,她决定加人电动汽车的行列。毕竟,特斯拉似乎出现在她居住的... 详细信息

高昂的价格、高昂的融资利率以及对续航里程的担忧,将导致2024年美国电动汽车的普及放缓。当黛比·梅滕雷特(Debbie Mettenleiter)的旧现代汽车在行驶了20万英里后需要更换时,她决定加人电动汽车的行列。毕竟,特斯拉似乎出现在她居住的圣地亚哥的每个红绿灯路口。甚至连她的女儿也有一辆。于是她付了250美元订金,准备买一辆特斯拉Model Y。

关键词：电动汽车续航里程红绿灯特斯拉里程焦虑圣地亚哥

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Comprehensive Optimization-based Techno-economic Assessment of Hybrid Renewable Electricity-hydrogen Virtual Power Plants

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Journal of Modern Power Systems and Clean Energy 2023年第2期11卷 553-566页

作者： James naughton Shariq Riaz Michael Cantoni Xiao-Ping Zhang Pierluigi Mancarella University of Melbourne(UoM) Australia University of Birmingham(UoB) UK University of Manchester UK

Hydrogen is being considered as an important option to contribute to energy system ***,currently its production from renewables is expensive compared with the methods that utilize fossil *** paper proposes a comprehensive optimization-based techno-economic assessment of a hybrid renewable electricity-hydrogen virtual power plant(VPP)that boosts its business case by co-optimizing across multiple markets and contractual services to maximize its profits and eventually deliver hydrogen at a lower net ***,multiple possible investment options are *** studies of VPP placement in a renewable-rich,congested area of the Australian network and based on real market data and relevant sensitivities show that multi-market participation can significantly boost the business case for cleaner *** highlights the importance of value stacking for driving down the cost of cleaner *** to the participation in multiple markets,all VPP configurations considered are found to be economically viable for a hydrogen price of 3 AUD$/kg(2.25 USD$/kg),which has been identified as a threshold value for Australia to export hydrogen at a competitive ***,if the high price volatility that has been seen in gas prices in 2022(and by extension electricity prices)continues,the flexibility of hybrid VPPs will further improve their business cases.

关键词： Virtual power plant techno-economic assessment electrolyser flexibility hydrogen multi-energy system optimal power flow

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The demands of the extra-time period of soccer:A systematic review

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Journal of Sport and Health Science 2022年第3期11卷 403-414页

作者： Adam Field Robert Joseph naughton Matthew Haines Steve Lui Liam David Corr Mark Russell Richard Michael Page Liam David Harper School of Human and Health Sciences University of HuddersfieldHuddersfieldHD13DHUK School of Social and Health Sciences Leeds Trinity UniversityBrownberrie LaneHorsforthLeedsLS185HDUK Department of Sport&Physical Activity Edge Hill UniversitySt.Helens RoadOrmskirkLancashireL394QPUK

Background:Soccer match-play is typically contested over 90 min;however,in some cup and tournament scenarios,when matches are tied,they proceed to an additional 30 min,which is termed "extra-time^(ET).This systematic review sought to appraise the literature available on 120-min of soccer-specific exercise,with a view to identifying practical recommendations and future research ***:The review was conducted according to the PRISMA *** researchers performed a systematic search of PubMed,CINAHL,and PsycINFO in May 2019,with the following keywords entered in various combinations:"soccer","football","extra-time","extra time","extratime","120 minutes","120 min","additional 30 minutes",and "additional 30 min".Results:The search yielded an initial 73 *** the screening process,11 articles were accepted for *** were subsequently organized into the following 5 categories:movement demands of ET,performance responses to ET,physiological and neuromuscular response during ET,nutritional interventions,and recovery and *** results highlighted that during competitive match-play,players cover5%-12% less distance relative to match duration(i.e.,meters per minute) during ET compared to the preceding 90 *** in technical performance(i.e.,shot speed,number of passes and dribbles) were also observed during ***,carbohydrate provision may attenuate and improve dribbling performance during ***,objective and subjective measures of recovery may be further compromised following ET when compared to 90 ***:Additional investigations are warranted to further substantiate these findings and identify interventions to improve performance during ET.

关键词： Movement demands Neuromuscular fatigue Nutritional intervention Performance Physiology

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Compression strategies for digital holograms in biomedical and multimedia applications

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Light(Advanced Manufacturing) 2022年第3期3卷 234-254页

作者： Peter Schelkens Ayyoub Ahar Antonin Gilles Raees Kizhakkumkara Muhamad Thomas J.naughton Cristian Perra Antonio Pinheiro Piotr Stepien Malgorzata Kujawinska Vrije Universiteit Brussel ETRO Dept.B-1050 BrusselsBelgium imec Digital and User-Centric Solutions(DUCS)B-3001 LeuvenBelgium Institute of Research&Technology b<>com RennesFranc Maynooth University Dept.of Computer ScienceMaynoothCo.KildareIreland University of Cagliari DIEEUdR CNITCagliariItaly Universidade da Beira Interior CovilhaPortugal Instituto de Telecomunicacoes CovilhaPortugal Warsaw University of Technology Institute of Micromechanics and Photonics02-525 WarsawPoland

While 60 years of successful application of holography is celebrated in this special issue,efficient representation and compression of holographic data has received relatively little attention in *** this observation,and particularly due to the digitization that is also penetrating the holographic domain,interest is growing on how to efficiently compress holographic data such that interactive exchange of content,as well as digital storage can be facilitated *** is a particular challenge,not only because of its interferometric nature and the various representation formats,but also the often extremely large data volumes involved in pathological,tomographic,or high-end visualization *** this paper,we provide an overview of the state of the art in compression techniques and corresponding quality metrics for various practical applications in digital *** also consider the future by analyzing the emerging trends for addressing the key challenges in this domain.

关键词： Holography Compression Image quality assessment Standardization

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基于风洞实验的风力机叶片气动负载计算方法

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太阳能学报 2016年第8期37卷 2068-2073页

作者：李迺璐穆安乐 Jonathan naughton 扬州大学水利与能源动力工程学院扬州225127 西安理工大学机械与精密仪器工程学院西安710048 美国怀俄明大学机械工程系拉勒米82072

提出一种基于风洞实验的风力机叶片气动负载计算方法。理论分析Beddoes-Leishman空间状态模型的非定常气动力特性,结合风洞实验数据和Beddoes-Leishman模型开发动态气动负载的数值计算程序。利用开发程序,分别详细计算DU97W300-10翼型... 详细信息

提出一种基于风洞实验的风力机叶片气动负载计算方法。理论分析Beddoes-Leishman空间状态模型的非定常气动力特性,结合风洞实验数据和Beddoes-Leishman模型开发动态气动负载的数值计算程序。利用开发程序,分别详细计算DU97W300-10翼型在不同攻角区间,即线性区、失速区和全区的非定常气动系数,分析每个区间内附着流和分离流对翼型动态气动特性的影响。结果表明,在所有攻角区域的气动参数计算结果均能很好地与理论分析结果达成一致,翼型风洞实验可有效保证特定翼型动态气动负载计算的准确性。

关键词：风洞实验动态气动负载 Beddoes-Leishman模型风力机叶片空间状态模型

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The role of the unfolded protein response in myelination

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Neural Regeneration Research 2016年第3期11卷 394-395页

作者： Michelle C.naughton Jill M.McMahon Una F.FitzGerald NCBES Galway Neuroscience CentreNational University of Ireland Galway

The production,transport and integration of myelin components into the membrane during development is a highly coordinated and regulated process that relies heavily on the endoplasmic reticulum（ER）,a sub-cellular organelle that is the principal site of membrane *** on the rough ER allow translation of proteins such as proteolipid protein（PLP）prior to correct folding,

关键词： endoplasmic translational reticulum prior folding assembly coordinated myelin correct tracts

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电动汽车税制存在漏洞美国消费者以租代买

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商业周刊（中文版） 2023年第9期 39-40页

作者： Keith naughton 汪泽(译) 不详

阿利斯泰尔·韦弗(Alistair Weaver)左右为难,他眼下开的特斯拉(Tesla)Model3的租约还有几个月即将到期,他不知道要不要再换一辆电动汽车。韦弗与妻子和两个孩子住在旧金山郊区,他喜欢开零排放的车子,以帮助减少污染。

关键词：电动汽车特斯拉零排放韦弗减少污染旧金山

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