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Preparation and Investigation of Arsenic Trioxide-loaded Polylactic Acid/Magnetic Hybrid Nanoparticles

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Chemical Research in Chinese Universities 2014年第2期30卷 326-332页

作者： SONG Xiaoli YOU juan WANG juan zhu Aiping JI Lijun GUO Rong College of Chemistry and Chemical Engineering Yangzhou University Yangzhou 225002 P.R.China

In order to improve the therapeutic index and reduce the side effects of As2O3 so as to expand its clinical utility to solid tumors,As2O3-loaded polylactic acid（PLA）/magnetic nanoparticles（PAMNs） were prepared and the preparation conditions were optimized by the improved double emulsion solvent-evaporation method to obtain as a drug delivery system with a high As2O3 encapsulation efficiency and homogeneous nanosized *** loading of magnetic nanoparticles into the nanoparticles was confirmed by Fourier transform infrared（FTIR） *** drug loading,encapsulation efficiency and releasing properties of As2O3 in PAMNs were investigated by atomic fluorescence spectrometer ***,the in vitro antitumor efficacy of free As2O3 and As2O3-PAMNs against SMMC-7721 cell line was ***,confocal fluorescence microscopy was conducted to reveal the celluar uptake of As2O3-loaded PAMNs by SMMC-7721 cell line.

关键词： As2O3 Magnetism Polylactic acid nanoparticle Drug release

Effect of Danshao Huaxian capsule on Gremlin and bone morphogenetic protein-7 expression in hepatic fibrosis in rats

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World Journal of Gastroenterology 2014年第40期20卷 14875-14883页

作者： Xue-Ke Zhao Ming-Liang Cheng Rong-Min Wu Yu-Mei Yao Mao Mu juan-juan zhu Bao-Fang Zhang Ming-Yu Zhou Department of Infectious Diseases Affiliated Hospital of Guiyang Medical College Department of B Ultrasound Maternal and Child Health Hospital of Guizhou Province

AIM: To observe the effect of Danshao Huaxian capsule (DHC) on the expression of Gremlin and bone morphogenetic protein-7 (BMP-7) in the liver of hepatic fibrosis rats.

关键词： Hepatic fibrosis Bone morphogenetic protein Gremlin Transforming growth factor Traditional Chinese herbs

Transforming growth factor beta-1 upregulates glucose transporter 1 and glycolysis through canonical and noncanonical pathways in hepatic stellate cells

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World Journal of Gastroenterology 2021年第40期27卷 6908-6926页

作者： Ming-Yu Zhou Ming-Liang Cheng Tao Huang Rui-Han Hu Gao-Liang Zou Hong Li Bao-Fang Zhang juan-juan zhu Yong-Mei Liu Yang Liu Xue-Ke Zhao Department of Internal Medicine Guizhou Medical UniversityGuiyang 550001Guizhou ProvinceChina Department of Infectious Diseases Affiliated Hospital of Guizhou Medical UniversityGuiyang 550004Guizhou ProvinceChina Clinical Laboratory Center Affiliated Hospital of Guizhou Medical UniversityGuiyang 550004Guizhou ProvinceChina

BACKGROUND Hepatic stellate cells(HSCs)are the key effector cells mediating the occurrence and development of liver fibrosis,while aerobic glycolysis is an important metabolic characteristic of HSC *** growth factor-β1(TGF-β1)induces aerobic glycolysis and is a driving factor for metabolic *** occurrence of glycolysis depends on a high glucose uptake *** transporter 1(GLUT1)is the most widely distributed glucose transporter in the body and mainly participates in the regulation of carbohydrate metabolism,thus affecting cell proliferation and ***,little is known about the relationship between TGF-β1 and GLUT1 in the process of liver fibrosis and the molecular mechanism underlying the promotion of aerobic glycolysis in *** To investigate the mechanisms of action of GLUT1,TGF-β1 and aerobic glycolysis in the process of HSC activation during liver *** Immunohistochemical staining and immunofluorescence assays were used to examine GLUT1 expression in fibrotic liver tissue.A Seahorse extracellular flux(XF)analyzer was used to examine changes in aerobic glycolytic flux,lactate production levels and glucose consumption levels in HSCs upon TGF-β1 *** mechanism by which TGF-β1 induces GLUT1 protein expression in HSCs was further explored by inhibiting/promoting the TGF-β1/mothersagainst-decapentaplegic-homolog 2/3(Smad2/3)signaling pathway and inhibiting the p38 and phosphoinositide 3-kinase(PI3K)/AKT signaling *** addition,GLUT1 expression was silenced to observe changes in the growth and proliferation of ***,a GLUT1 inhibitor was used to verify the in vivo effects of GLUT1 on a mouse model of liver *** GLUT1 protein expression was increased in both mouse and human fibrotic liver *** addition,immunofluorescence staining revealed colocalization of GLUT1 and alpha-smooth muscle actin proteins,indicating that GLUT1 expression was related to the development of

关键词： Gene regulation Glycolysis Liver fibrosis Glucose transporter 1 Transforming growth factor-β1

Bone morphogenetic protein-7 represses hepatic stellate cell activation and liver fibrosis via regulation of TGF-β/Smad signaling pathway

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World Journal of Gastroenterology 2019年第30期25卷 4222-4234页

作者： Gao-Liang Zou Shi Zuo Shuang Lu Rui-Han Hu Yin-Ying Lu Jing Yang Kai-Sheng Deng Ye-Ting Wu Mao Mu juan-juan zhu Jing-Zhang Zeng Bao-Fang Zhang Xian Wu Xue-Ke Zhao Hai-Yang Li Department of Infectious Diseases Affiliated Hospital of Guizhou Medical UniversityGuiyang 550004Guizhou ProvinceChina Department of Hepatobiliary Surgery Affiliated Hospital of Guizhou Medical UniversityGuiyang 550004Guizhou ProvinceChina Comprehensive Liver Cancer Center 302 HospitalBeijing 100039China

BACKGROUND Liver fibrosis is a refractory disease whose persistence can eventually induce cirrhosis or even liver *** liver fibrosis is reversible by *** a member of the transforming growth factor-beta(TGF-β)superfamily,bone morphogenetic protein 7(BMP7)has anti-liver fibrosis ***,little is known about BMP7 expression changes and its potential regulatory mechanism as well as the relationship between BMP7 and TGF-βduring liver *** addition,the mechanism underlying the anti-liver fibrosis function of BMP7 needs to be further *** To investigate changes in the dynamic expression of BMP7 during liver fibrosis,interactions between BMP7 and TGF-β1,and possible mechanisms underlying the anti-liver fibrosis function of *** Changes in BMP7 expression during liver fibrosis and the interaction between BMP7 and TGF-β1 in mice were *** BMP7 was used to treat mouse primary hepatic stellate cells(HSCs)to observe its effect on activation,migration,and proliferation of HSCs and explore the possible mechanism underlying the anti-liver fibrosis function of *** with liver fibrosis received exogenous BMP7 intervention to observe improvement of liver fibrosis by using Masson’s trichrome staining and detecting the expression of the HSC activation indicator alpha-smooth muscle actin(α-SMA)and the collagen formation associated protein type I collagen(Col I).Changes in the dynamic expression of BMP7 during liver fibrosis in the human body were further *** In the process of liver fibrosis induced by carbon tetrachloride(CCl4)in mice,BMP7 protein expression first increased,followed by a decrease;there was a similar trend in the human *** process was accompanied by a sustained increase in TGF-β1 protein *** vitro experiment results showed that TGF-β1 inhibited BMP7 expression in a time-and dose-dependent *** contrast,high doses of exogenous BMP7 inhibited TGF-β1-induced activation

关键词： Liver fibrosis Bone morphogenetic protein Transforming growth factor Hepatic stellate cells

Sequence-activated chemiluminescence probes: programmable illumination for high-contrast in vivo sensing and imaging

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Science China Chemistry 2025年第01期 26-34页

作者： Yutao Zhang juan Li Wei-Hong zhu Zhiqian Guo Shanghai Key Laboratory of Functional Materials Chemistry Joint International Research Laboratory of Precision Chemistry and Molecular Engineering Institute of Fine Chemicals School of Chemistry and Molecular Engineering East China University of Science and Technology State Key Laboratory of Bioreactor Engineering Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell MetabolismEast China University of Science & Technology

Sequence-activated chemiluminescence(CL) technique offers a unique approach to detecting specific biomolecules or cellular events with high sensitivity and spatial resolution. The fundamental principle behind sequence-activated CL probes involves tailoring highly-controllable CL signals in response to specific sequences or events of interest, thus enabling high-contrast signals under complex physiological conditions. This sequential activation could be triggered by enzymes, reactive oxygen species(ROS), biothiols or changes in the microenvironment. These unique sequence-activated CL probes bestow several advantages, including minimal background, deep-tissue penetration, and the ability of non-invasive or minimally invasive detection. The sequence-activated CL techniques represent a significant advancement in molecular imaging and diagnostics. In this mini-review, we focus on the latest developments in sequential CL probes, addressing the technical challenges and discussing the future trajectory by harnessing CL signaling and molecular recognition.

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The Mammalian Target of Rapamycin and DNA methyltransferase 1 axis mediates vascular endothelial dysfunction in response to disturbed flow

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The Mammalian Target of Rapamycin and DNA methyltransferase ...

宫颈上皮内瘤变宫颈锥切术后二次手术术式探讨及临床疗效分析

中国生理学会基质生物学专业委员会第二次全国基质生物学学术会议

作者：张云鹏 Yi-Tao Huang Wei Pang juan-juan zhu Yue-Feng Liu Jing Zhou 北京大学医学部 Department of Physiology and Pathophysiology School of Basic Medical SciencesPeking University

Objective:Vascular endothelial cells(ECs)are constantly exposed to shear stress induced by circulatingblood flow,but the mechanism of flow-pattern-specific modulation of endothelial function remains *** study aims to demonstrate the role of DNA methyltransferase 1(DNMT1)-dependent DNA methylation in the flow-regulation of endothelial function as well as the detailed signaling mechanism.

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中国实用妇科与产科杂志 2018年第3期34卷 298-303页

作者：刘淼齐跃刘娟娟李潇朱连成刘川林蓓中国医科大学附属盛京医院妇产科辽宁沈阳110004

目的探讨宫颈上皮内瘤变(CIN)宫颈锥切术后需二次手术且要求保留子宫及生育功能患者的术式选择及临床疗效。方法选取2012年1月至2017年5月中国医科大学附属盛京医院收治的既往因CIN行宫颈锥切术[环形电切术(LEEP)或冷刀锥切],术后由于... 详细信息

目的探讨宫颈上皮内瘤变(CIN)宫颈锥切术后需二次手术且要求保留子宫及生育功能患者的术式选择及临床疗效。方法选取2012年1月至2017年5月中国医科大学附属盛京医院收治的既往因CIN行宫颈锥切术[环形电切术(LEEP)或冷刀锥切],术后由于切缘阳性、术后病理升级、随访中发现病变残留或复发以及持续细胞学异常需再次手术治疗,且要求行保留子宫及生育功能手术的患者35例。二次手术指征包括:锥切术后病理提示切缘阳性(7例),病理较活检升级或可疑早期浸润(8例),术后病理为高度鳞状上皮内病变(CINⅢ)且患者自身对癌症过分恐惧要求手术(1例),随访中发现病灶残留或复发(17例,其中病灶残留5例,复发12例),持续细胞学异常(2例)。术式采用宫颈锥切术23例,宫颈切除术+改良缝合术12例。回顾性分析上述患者的临床治疗过程、疗效及预后。结果 35例患者平均年龄40.85岁(27~56岁),手术时间平均23.62min(10~37 min),术中出血量平均12m L(5~30m L)。手术均顺利,均无副损伤及大出血等术中、术后并发症发生,术后宫颈创面均愈合良好。术后病理较术前病理降级17例,升级6例,与术前病理相符12例。全部患者术后每3~6个月随访1次,中位随访时间23个月(3~63个月),均恢复良好,无一例复发。二次手术术前未绝经患者32例,术后均无月经改变,且2例患者术后成功妊娠,均顺利妊娠至足月剖宫产分娩。结论对于宫颈锥切术后需再次手术的CIN患者,二次宫颈锥切术、宫颈切除术是安全有效的诊治方法,手术创伤小,术中出血少,术后恢复快,同时能够保留子宫及生育功能。

关键词：宫颈锥切术宫颈切除术宫颈上皮内瘤变

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鼻咽癌侯选抑瘤基因BRD7原核表达载体的构建及其表达( 英文)

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生物化学与生物物理进展 2002年第4期29卷 631-634页

作者：聂新民张必成向娟娟朱诗国周鸣董利余鹰李小玲李桂源中南大学湘雅医学院肿瘤研究所长沙410078

BRD7基因是一个鼻咽癌侯选抑瘤基因 ,为了构建BRD7基因的原核表达载体并使其在大肠杆菌得到表达 ,设计了带有SalⅠ ,NotⅠ酶切位点的引物 ,以已构建好的质粒 pGEM TEasy/BRD7为模板 ,用PCR扩增出BRD7基因的完整阅读框架 ,并用SalⅠ ,No... 详细信息

BRD7基因是一个鼻咽癌侯选抑瘤基因 ,为了构建BRD7基因的原核表达载体并使其在大肠杆菌得到表达 ,设计了带有SalⅠ ,NotⅠ酶切位点的引物 ,以已构建好的质粒 pGEM TEasy/BRD7为模板 ,用PCR扩增出BRD7基因的完整阅读框架 ,并用SalⅠ ,NotⅠ酶切PCR产物和原核表达载体PGEX 4T 2 ,然后用T4DNA连接酶将其连接 ,得到重组表达质粒PGEX 4T 2 /BRD7,经双酶切鉴定和测序验证 ,表达载体构建正确 .重组表达质粒转化感受态大肠杆菌Jm10 5后用IPTG诱导 ,成功表达了一分子质量约为 90ku的融合蛋白 ;37℃诱导 4h后 ,SDS 聚丙烯酰胺凝胶 (PAGE)电泳后 ,经扫描分析该融合蛋白产量占菌体蛋白总量 2 8 4 8% ,蛋白质印迹 (Western blot)证实了该融合蛋白的表达获得成功 .这为BRD7基因的蛋白纯化及抗体制备 ,进一步开展其功能研究奠定了基础 .

关键词：鼻咽癌侯选抑瘤基因 BRD7 构建表达原核表达载体

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猪PD-1及其配体PD-L1和PD-L2 SYBR Green Ⅰ Real-time PCR检测方法的建立及应用

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中国畜牧兽医 2016年第11期43卷 2892-2899页

作者：岳锋周娟娟朱艳平李鹏孙国鹏王选年新乡学院生命科学技术学院新乡453003 新乡学院生物技术研究所新乡453003

本试验旨在研究猪PD-1及其配体在疾病状态下的转录水平,建立检测猪PD-1及其配体的Real-time PCR方法。根据GenBank中猪PD-1、PD-L1和PD-L2基因序列,分别设计3对特异性引物,PCR扩增目的基因片段,回收目的基因片段与pMD18-T连接后转化至... 详细信息

本试验旨在研究猪PD-1及其配体在疾病状态下的转录水平,建立检测猪PD-1及其配体的Real-time PCR方法。根据GenBank中猪PD-1、PD-L1和PD-L2基因序列,分别设计3对特异性引物,PCR扩增目的基因片段,回收目的基因片段与pMD18-T连接后转化至大肠杆菌DH5α感受态细胞中,提取重组质粒,经PCR及测序鉴定正确,作为标准品绘制标准曲线,并进行特异性和重复性检测。结果显示,Real-time PCR的Ct值与模板浓度对数呈良好的线性反比关系,相关系数R2>0.99,熔解曲线出现狭窄单一峰;Real-time PCR产物经琼脂糖凝胶电泳,呈单一目的条带,无引物二聚体,特异性强;组内和组间重复性检测结果显示,组内和组间的变异系数均小于3%,重复性好;用建立的Real-time PCR方法检测猪自然感染PCV2后PD-1及其配体转录水平,PD-L1和PD-L2转录水平极显著或显著升高(P0.05)。本试验建立了检测猪PD-1、PDL1和PD-L2mRNA的Real-time PCR方法并进行了初步应用,为猪PD-1、PD-L1和PD-L2基因表达模式研究奠定基础。

关键词： Real-time PCR PD-1 PD-L1 PD-L2

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