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Journal of Pharmaceutical Analysis 2022年第1期12卷 113-121页

作者： juan tan Shiyue Wu Qingqing Cai Yi Wang Pu Zhang Engineering Research Center for Biotechnology of Active Substances Ministry of EducationChongqing Key Laboratory of Green Synthesis and ApplicationsCollege of ChemistryChongqing Normal UniversityChongqing401331China Chongqing Research Center for Pharmaceutical Engineering College of PharmacyChongqing Medical UniversityChongqing400016China

Regulating the catalytic activity of nanozymes is significant for their applications in various ***,we demonstrate a new strategy to achieve reversible regulation of the nanozyme’s activity for sensing *** strategy involves the use of zero-dimensional MoS;quantum dots(MQDs)as the building blocks of nanozymes which display very weak peroxidase(POD)-like ***,such POD-like activity of the MQDs largely enhances in the presence of Fe;while diminishes with the addition of captopril *** investigations identify the mechanism of Fe;-mediated aggregation-induced enhancement of the POD-like activity and the inhibitory effect of captopril on the enhancement,which is highly dependent on their *** on this finding,a colorimetric method for the detection of captopril is *** sensing approach exhibits the merits of simplicity,rapidness,reliability,and low cost,which has been successfully applied in quality control of captopril in pharmaceutical ***,the present sensing platform allows smartphone read-out,which has promising applications in point-of-care testing devices for clinical diagnosis and drug analysis.

关键词： Nanozyme Aggregation Peroxidase Captopril Colorimetric detection Smartphone

Cobalt phthalocyanine-graphene complex for electro-catalytic oxidation of dopamine

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Journal of Natural Gas Chemistry 2012年第3期21卷 265-269页

作者： Jinghe Yang Di Mu Yongjun Gao juan tan Anhui Lu Ding Ma Faculty of Chemical Environmental and Biological Science and technologyDalian University of TechnologyDalian 116024LiaoningChina Beijing National Laboratory for Molecular Sciences College of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China

Cobalt phthalocyanine-graphene （CoPc-Gr） complex are fabricated through 7r-Tr interaction of each components, with CoPc adsorbed/inserted on/in the graphene sheets. The obtained complex could be used in the electro-chemical detection of various medicines. CoPc-Gr modified glassy electrode shows excellent response to the electro-oxidation of dopamine （DA） and ascorbic acid （AA）, much better than those of CoPc, graphene oxide （GrO） or graphene （Gr） modified electrode. Significantly, the detection of dopamine is a diffusion-controlled process, highly selective, and has a low detection limit and broad linear range.

关键词： cobalt phthalocyanine-graphene dopamine electro-oxidation selective detection

Austenite Grain Refinement by Reverse α′→γ Transformation in Metastable Austenitic Manganese Steel

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Journal of Iron and Steel Research International 2015年第2期22卷 157-162页

作者： Jia-tao ZHANG Yu-guang ZHAO juan tan Xiao-feng XU Key Laboratory of Automobile Materials of Ministry of Education Department of Materials Science and EngineeringJilin University

Microstructure of metastable austenitic manganese steel after reverse transformation treatment was investi gated using optical microscopy, X ray diffraction （XRD）, electrical resistivity and hardness testing. Austenite grain refinement was successfully achieved by a two-step heat treatment. First, martensite was produced by cooling the so- lution-treated samples to --196 ℃. Then, the deep cryogenic treated samples were heated to 850 ℃ upon slow or rapid heating. The mean size of original austenite grain was about 400 fire. But the mean size of equiaxed reversion austenite was refined to 50 μm. Microstructure evolution and electrical resistivity change showed that martensite plates underwent tempering action upon slow heating, and the residual austenite was decomposed, resulting in the formation of pearlite nodules at the austenite grains boundaries. The refinement mechanism upon slow heating is the diffusion-controlled nucleation and growth of austenite. However, the reverse transformation upon rapid heating was predominated by displacive manner. The residual austenite was not decomposed. The plate α-phase was carbon-super- saturated until the starting of reverse transformation. The reverse transformation was accompanied by surface effect, resulting in the formation of plate austenite with high density dislocations. The refinement mechanism upon rapid heating is the recrystallization of displacive reversed austenite.

关键词： grain refinement displacive reverse transformation recrystallization metastable austenitic manganese steel

Microstructure Refinement and Property Improvement of Metastable Austenitic Manganese Steel Induced by Electropulsing

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Journal of Iron and Steel Research International 2014年第7期21卷 685-689页

作者： Yu-guang ZHAO Jia-tao ZHANG juan tan Bing-dong MA Key Laboratory of Automobile Materials of Ministry of Education Department of Materials Science and EngineeringJilin University

Grain refinement efficiency of electropulsing treatment （EPT） for metastable austenitic manganese steel was investigated. The mean grain size of original austenite is 300 ptm. However, after EPT, the microstructure ex hibits a bimodal grain size distribution, and nearly 70vol. % grains are less than 60 /Lm. The refined austenite results in ultrafine martensitic microstrncture. The tensile strengths of refined austenitic and martensitic microstructures were improved from 495 to 670, and 794 to 900 MPa respectively. The fine grained materials possess better fracture toughness. The work hardening capacity and wear resistance of the refined austenitic microstructure are improved. The reasonable mechanism of grain refinement is the combination of accelerating new phase nucleation and restraining the growth of neonatal austenitic grain during reverse transformation and rapid recrystallization induced by electropulsing.

关键词： eleetropulsing grain refinement metastable austenitic manganese steel

Bacterial community response to petroleum contamination in brackish tidal marsh sediments in the Yangtze River Estuary, China

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Journal of Environmental Sciences 2021年第1期33卷 160-167页

作者： Min Wang Chenyan Sha Jian Wu Jinghua Su Jianqiang Wu Qing Wang juan tan Shenfa Huang Research Institute of Natural Ecology Conseruation Shanghai Academy of Enuvironmental Sciences508 Qinzhou RoadShanghai 200233China

The brackish tidal marsh in the Baimaosha area of the Yangtze River Estuary was severely contaminated by 400 tons of heavy crude petroleum from a tanker that sank in December *** spill accident led to severe environmental damage owing to its high toxicity,persistence and wide *** communities play vital roles in petroleum degradation in marsh ***,taxonomic analysis,high-throughput sequencing and 16 S rRNA functional prediction were used to analyze the structure and function of microbial communities among uncontaminated(CK),lightly polluted(LP),heavily polluted(HP),and treated(TD)*** bacterial communities responded with increased richness and decreased diversity when exposed to petroleum *** dominant class changed from Deltaproteobacteria to Gammaproteobacteria after petroleum *** phylum Firmicutes increased dramatically in oil-enriched sediment by 75.78%,346.19%and 267.26%in LP,HP and TD,*** of the suspected oil-degrading genera,Dechloromonas,increased the most in oil-contaminated sediment,by 540.54%,711.27%and 656.78%in LP,HP and TD,*** protease,quinate dehydrogenase(quinone)and glutathione-independent formaldehyde dehydrogenase,three types of identified enzymes,increased enormously with the increasing petroleum *** conclusion,petroleum contamination altered the community composition and microorganism structure,and promoted some bacteria to produce the corresponding degrading ***,the suspected petroleum-degrading genera should be considered when restoring oil-contaminated sediment.

关键词： Brackish marsh sediment Petroleum contamination Bacterial community Gene enzyme

Comparison of clinical outcomes and postoperative recovery between two open heart surgeries:minimally invasive right subaxillary vertical thoracomy and traditional median sternotomy

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Asian Pacific Journal of Tropical Medicine 2014年第8期7卷 625-629页

作者： Chuan-Xian Hu juan tan Sheng Chen Hui Ding Zhi-Wei Xu Department of Cardiothoracic Surgery Huai'an First People's Hospital Affiliated to Nanjing Medical University Department of Cardiothoracic Surgery Zhejiang Normal University

Objective: To compare the clinical outcomes of minimally invasive right subaxillary vertical thoracotomy and traditional median stemotomy through right atrium in treatment of common congenital heart diseases. Methods: Clinical data of 59 cases of common congenital heart diseases treated with minimally invasive right axillary vertical thoracotomy from May, 2011 to February, 2013 and 77 cases of same diseases with traditional median stemotomy in the past three years were retrospectively analyzed, including atrial septal defect, membranous ventricular septal defect and partial endocardial cushion defect. The results were compared from the two groups, including the time for operation and cardiopulmonary bypass, amount of blood transfusion, postoperative drainage, ventilation time, hospital stay, and prognosis. Results: No severe complications happened in both groups, like deaths or secondary surgery caused by bleeding. No significant differences were in CPB time and postoperative ventilator time between groups (P>0.05), while for all of the operative time, the length of incision, postoperative drainage and hospital stay, minimally invasive right axillary vertical thoracotomy was superior to median stemotomy, with statistically significant differences (P<0.05). In six month followup after operation, no complications of residual deformity and pericardial effusion were found in both groups by doing echocardiography, but mild pectus carinatum was found in 8 patients in the traditional median sternotomy group (traditional group), whereas patients in another group were well recovered. Conclusions: Minimally invasive right subaxillary vertical thoracotomy for common congenital heart diseases is as safe as traditional median stemotomy, without the increasing incidence of postoperative complications. Additionally, compared with traditional median stemotomy, minimally - invasive right subaxillary vertical thoracotomy is better in the aspects of hidden incision, appearance, and po

关键词： Minimally invasive surgery Congenital heart diseases Right subaxillary vertical thoracotomy Traditional median stemotomy Cardiopulmonary bypass

Frequeney-Locking in a Bpatially Extended Predator-Prey Model

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Chinese Physics Letters 2011年第1期28卷 215-217页

作者： YU Cun-juan tan Ying-Xin School of Chemical Engineering and Environmental North University of China Taiyuan 030051

The study is concerned with the effect of variable dispersal rates on Turing instability of a spatial Holling-tanner system. A series of numerical simulations show that the oscillatory Turing pattern can emerge due to period diffusion coefficient. Moreover, we find that when the amplitude is above a threshold, 1： 1 frequency-locking oscillation can be obtained. The results show that period diffusion coefficient plays an important role on the pattern formation in the predator-prey system.

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Polyunsaturated linolenoyl-Co A modulates ERF-Ⅶ mediated hypoxia signaling in Arabidopsis

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Journal of Integrative Plant Biology 2020年第3期62卷 330-348页

作者： Ying Zhou Wei-juan tan Li-juan Xie Hua Qi Yi-Cong Yang Li-Ping Huang Yong-Xia Lai Yi-Fang tan De-Mian Zhou Lu-Jun Yu Qin-Fang Chen Mee-Len Chye Shi Xiao State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant ResourcesSchool of Life SciencesSun Yat-Sen UniversityGuangzhou 510275China School of Biological Sciences The University of Hong KongPokfulamHong Kong 999077China

In plants,submergence from flooding causes hypoxia,which impairs energy production and affects plant growth,productivity,and *** Arabidopsis,hypoxia induces nuclear localization of the group VII ethylene-responsive transcription factor RELATED TO AP2.12(RAP2.12),following its dissociation from the plasma membrane-anchored ACYL-COA BINDING PRO-TEIN1(ACBP1)and ***,we show that polyunsaturated linolenoyl-Co A(18:3-Co A)regulates RAP2.12release from the plasma *** caused a significant increase in 18:3-Co A,but a significant decrease in 18:0-,18:1-,and 18:2-Co *** of 18:3-Co A promoted nuclear accumulation of the green fluorescent protein(GFP)fusions RAP2.12-GFP,HYPOXIA-RESPONSIVE ERF1-GFP,and RAP2.3-GFP,and enhanced transcript levels of hypoxia-responsive *** with decreased ACBP1 and ACBP2(acbp1 ACBP2-RNAi,produced by ACBP2 RNA interference in the acbp1 mutant)had reduced tolerance to hypoxia and impaired 18:3-Co A-induced expression of hypoxia-related *** knockout mutants and overexpression lines of LONG-CHAIN ACYL-COA SYNTHASE2(LACS2)and FATTY ACID DE-SATURASE 3(FAD3),the acyl-Co A pool size and 18:3-Co A levels were closely related to ERF-VII-mediated signaling and hypoxia *** findings demonstrate that polyunsaturation of long-chain acyl-Co As functions as important mechanism in the regulation of plant hypoxia signaling,by modulating ACBP–ERF-VII dynamics.

关键词： Arabidopsis ERF impaired

Bombyx mori U-shaped regulates the melanization cascade and immune response via binding with the Lozenge protein

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Insect Science 2022年第3期29卷 704-716页

作者： Kui Zhang juan tan Xiangwei Hao Houyi tang Muhammad Nadeem Abbas Jingjing Su Yongyue Su Hongjuan Cui State Key Laboratory of Silkworm Genome Biology College of SericultureTextile and Biomass SciencesSouthwest UniversityChongqing400716China Chongqing Reproductive and Genetics Institute Chongqing Obstetrics and Gynecology HospitalChongqing400013China Department of Orthopaedic 920th Hospital of Joint Logistics Support Force of Chinese People’s Liberation ArmyKunming650032China Cancer Center Reproductive Medicine CenterMedical Research InstituteSouthwest UniversityChongqing400716China

Zinc finger protein,an important transcription factor,regulates gene expression associated with various physiological and pathological processes.U-shaped,belong to the Friend of GATA(FOG)transcription factor,plays a crucial role in hematopoiesis by interacting with the GATA transcription factor as a ***,little is known about its functions in *** the present study,a U-shaped cDNA was identified and characterized from the silkworm Bombyx mori and its potential roles in innate immunity *** predicted silkworm U-shaped amino acid sequence contained a classical nuclear localization signal(NLS)motif“GESSPKRRRR”at position 45CU459,and arginine residues at position 456 and 478 are the critical sites of the NLS.U-shaped mRNA was detected in all tested tissues of the ***;however,the highest levels were found in the hemocytes.U-shaped mRNA expression levels were upregulated in the hemocyte after the Escherichia coli and Staphylococcus aureus ***,U-shaped knockdown significantly reduced the melanization process and suppressed the expression of melanization-associated genes,including PPO1,PPO2,PPAE and *** addition,U-shaped interacts with Lozenge protein to regulate the innate immune response of the *** results revealed that U-shaped binds directly to Lozenge protein to modulate the melanization process and innate immune responses in silkworm.

关键词： Bombyx mori Lozenge melanization response silkworm U-shaped

Treatment of End-Stage Coronary Artery Disease with the Mode of Combination of Disease and Syndrome:A Case Report

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Chinese Journal of Integrative Medicine 2021年第6期27卷 461-464页

作者： BAI Rui-na WANG Pei-li GU Feng tan juan WANG Cheng-long CHEN Ke-ji Cardiovascular Diseases Center Xiyuan HospitalChina Academy of Chinese Medical SciencesNational Clinical Research Center for Chinese Medicine CardiologyBeijing(100091)China

Coronary artery disease(CAD)remains the leading cause of mortality *** advanced pharmacologic development and invasive revascularization therapy,a small but not insignificant number of patients show progression to end... 详细信息

关键词： Coronary mortality therapy