Bombyx mori U-shaped regulates the melanization cascade and immune response via binding with the Lozenge protein
作者机构：State Key Laboratory of Silkworm Genome BiologyCollege of SericultureTextile and Biomass SciencesSouthwest UniversityChongqing400716China Chongqing Reproductive and Genetics InstituteChongqing Obstetrics and Gynecology HospitalChongqing400013China Department of Orthopaedic920th Hospital of Joint Logistics Support Force of Chinese People’s Liberation ArmyKunming650032China Cancer CenterReproductive Medicine CenterMedical Research InstituteSouthwest UniversityChongqing400716China
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2022年第29卷第3期
页 面：704-716页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学]
基 金：This work was supported by the National Natural Science Foundation of China(31802142) the Doctorial Start-up Fund of Southwest University(SWU120019,020023) the Fundamental Research Funds for the Central Universities(XDJK2019C089) the China Postdoctoral Science Foundation(2019T120801 and 2017M620408) the Opening Foundation of State Key Laboratory of Silkworm Genome Biology(SKLSGB-ORP202003)
主 题：Bombyx mori Lozenge melanization response silkworm U-shaped
摘 要：Zinc finger protein,an important transcription factor,regulates gene expression associated with various physiological and pathological processes.U-shaped,belong to the Friend of GATA(FOG)transcription factor,plays a crucial role in hematopoiesis by interacting with the GATA transcription factor as a ***,little is known about its functions in *** the present study,a U-shaped cDNA was identified and characterized from the silkworm Bombyx mori and its potential roles in innate immunity *** predicted silkworm U-shaped amino acid sequence contained a classical nuclear localization signal(NLS)motif“GESSPKRRRRat position 45CU459,and arginine residues at position 456 and 478 are the critical sites of the NLS.U-shaped mRNA was detected in all tested tissues of the ***;however,the highest levels were found in the hemocytes.U-shaped mRNA expression levels were upregulated in the hemocyte after the Escherichia coli and Staphylococcus aureus ***,U-shaped knockdown significantly reduced the melanization process and suppressed the expression of melanization-associated genes,including PPO1,PPO2,PPAE and *** addition,U-shaped interacts with Lozenge protein to regulate the innate immune response of the *** results revealed that U-shaped binds directly to Lozenge protein to modulate the melanization process and innate immune responses in silkworm.
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