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Science China Chemistry 2025年第01期 26-34页

作者： Yutao Zhang juan Li Wei-Hong Zhu Zhiqian Guo Shanghai Key Laboratory of Functional Materials Chemistry Joint International Research Laboratory of Precision Chemistry and Molecular Engineering Institute of Fine Chemicals School of Chemistry and Molecular Engineering East China University of Science and Technology State Key Laboratory of Bioreactor Engineering Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell MetabolismEast China University of Science & Technology

Sequence-activated chemiluminescence(CL) technique offers a unique approach to detecting specific biomolecules or cellular events with high sensitivity and spatial resolution. The fundamental principle behind sequence-activated CL probes involves tailoring highly-controllable CL signals in response to specific sequences or events of interest, thus enabling high-contrast signals under complex physiological conditions. This sequential activation could be triggered by enzymes, reactive oxygen species(ros), biothiols or changes in the microenvironment. These unique sequence-activated CL probes bestow several advantages, including minimal background, deep-tissue penetration, and the ability of non-invasive or minimally invasive detection. The sequence-activated CL techniques represent a significant advancement in molecular imaging and diagnostics. In this mini-review, we focus on the latest developments in sequential CL probes, addressing the technical challenges and discussing the future trajectory by harnessing CL signaling and molecular recognition.

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Software Project Management Approaches for Global Software Development： A Systematic Mapping Study

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Tsinghua Science and Technology 2018年第6期23卷 690-714页

作者： Manal El Bajta Ali Idri Joaquín Nicolás ros José Luis Fernández-Alemán juan Manuel Carrillo de Gea Félix García Ambrosio Toval Software Project Management Research Team Ecole Nationale SuperieuredInformatique et d'Analyse des Systemes (ENSIAS)Mohammed V University Rabat 713 Morocco Department of Informaticay Sistemas University of Murcia Murcia 30100 Spain ALARCOS Research GroupDepartment of Information Systems and Technologies University of Castilla-La Mancha (UCLM) Ciudad Real13071 Spain

Global Software Development （GSD） is a well established field of software engineering with the benefits of a global environment. Software Project Management （SPM） plays a key role in the success of GSD. As a result, the need has arisen to study and evaluate the downsides of SPM for GSD, to thereby pave the way for the development of new methods, techniques, and tools with which to tackle them. This paper aims to identify and classify research on SPM approaches for GSD that are available in the literature, to identify their current weaknesses and strengths, and to analyze their applications in industry. We performed a Systematic Mapping Study （SMS） based on six classification criteria. Eighty-four papers were selected and analyzed. The results indicate that interest in SPM for GSD has been increasing since 2006. As a class of approaches, the most frequently reported methods （40%） are those used for coordination, planning, and monitoring, along with estimation techniques that can be used to better match a distributed project. SPM for GSD requires further investigation by researchers and practitioners, particularly with respect to cost and time estimations. These findings will help overcome the challenges that must to be considered in future SPM research for GSD, especially regarding collaboration and time-zone differences.

关键词： Software Project Management (SPM) Global Software Development (GSD) SPM approaches Systematic Mapping Study (SMS)

Chaetocin reactivates the lytic replication of Epstein-Barr virus from latency via reactive oxygen species

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Science China(Life Sciences) 2017年第1期60卷 66-71页

作者： Shilun Zhang juan Yin Jiang Zhong Department of Microbiology and Microbial Engineering School of Life Sciences Fudan University Shanghai 200438 China

Oxidative stress, regarded as a negative effect of free radicals in vivo, takes place when organisms suffer from harmful stimuli. Some viruses can induce the release of reactive oxygen species （ros） in infected cells, which may be closely related with their pathogenicity. In this report, chaetocin, a fimgal metabolite reported to have antimicrobial and cytostatic activity, was studied for its effect on the activation of latent Epstein-Barr virus （EBV） in B95-8 cells. We found that chaetocin remarkably up-regulated EBV lytic transcription and DNA replication at a low concentration （50 nmol L-l）. The activation of latent EBV was accompanied by an increased cellular ros level. N-acetyl-L-cysteine （NAC）, an ros inhibitor, suppressed chaetocin-induced EBV activation. Chaetocin had little effect on histone H3K9 methylation, while NAC also significantly reduced H3K9 methylation. These results suggested that chaetocin reactivates latent EBV primarily via ros pathways.

关键词： Epstein-Barr virus reactive oxygen species chaetocin

Compound 3k治疗骨关节炎:调控氧化应激通路改善软骨细胞糖酵解的作用机制

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中国组织工程研究 2025年第2期29卷 363-370页

作者：郭素蓉曹士盛穆星彤杨青张娟天津医科大学口腔医学院天津市300041 天津医科大学口腔医院口腔修复科天津市300041

背景:骨关节炎现已被认为是一种代谢性疾病,既往研究表明糖酵解在骨关节炎的发生发展中起重要作用。Compound 3k作为一种新型糖酵解小分子抑制剂,具有抗炎及抗肿瘤等功效,因此可靶向糖酵解,有望为骨关节炎治疗提供新的思路。目的:基于... 详细信息

背景:骨关节炎现已被认为是一种代谢性疾病,既往研究表明糖酵解在骨关节炎的发生发展中起重要作用。Compound 3k作为一种新型糖酵解小分子抑制剂,具有抗炎及抗肿瘤等功效,因此可靶向糖酵解,有望为骨关节炎治疗提供新的思路。目的:基于缺氧诱导因子1α/活性氧的氧化应激通路探究Compound 3k在糖酵解过度活跃所导致的骨关节炎中的作用机制。方法:取对数生长期的ATDC5成软骨细胞,用10 ng/mL白细胞介素1β作用24 h诱导骨关节炎体外细胞模型,以CCK-8法检测不同浓度(0.25,0.5,1,2.5,5,10,15μmol/L)Compound 3k的细胞毒性,选出合适浓度进行后续实验。将软骨细胞随机分为对照组、模型组、治疗组,模型组以10 ng/mL的白细胞介素1β诱导,治疗组以Compound 3k预刺激2 h后与白细胞介素1β共培养,用CCK-8法检测各组细胞增殖情况;用ELISA试剂盒检测各组细胞炎症水平;用试剂盒检测各组细胞活性氧、细胞外乳酸脱氢酶及葡萄糖含量;qRT-PCR及Western blot检测相关炎症因子白细胞介素6、肿瘤坏死因子α及糖酵解相关基因葡萄糖转运蛋白1、甘油醛3-磷酸脱氢酶、单羧酸转运蛋白1和缺氧诱导因子1α的表达水平。结果与结论:①与对照组相比,模型组细胞增殖活性下降、糖酵解水平活跃,表现为细胞外乳酸脱氢酶含量增加(P<0.001),葡萄糖含量减少(P<0.001),相关炎症因子白细胞介素6(P<0.0001)及肿瘤坏死因子α(P<0.001),糖酵解相关基因葡萄糖转运蛋白1(P<0.001)、甘油醛3-磷酸脱氢酶(P<0.001)、单羧酸转运蛋白1(P<0.001)及缺氧诱导因子1α(P<0.001)的表达水平均上调,并伴随氧化应激,活性氧过量产生。②与模型组相比,Compound 3k的治疗有效提高细胞增殖活性,抑制过度活跃的糖酵解水平的同时,抑制了骨关节炎软骨细胞炎症(P<0.001)及糖酵解相关基因的表达(P<0.001),且抑制氧化应激,缺氧诱导因子1α的表达水平下调(P<0.0001),活性氧水平下降。③上述结果证实,Compound 3k抑制了白细胞介素1β诱导的软骨细胞炎症,其机制可能与糖酵解及缺氧诱导因子1α/活性氧介导的氧化应激有关。

关键词：骨关节炎糖酵解 Compound 3k 氧化应激 HIF-1α/ros ATDC5细胞葡萄糖代谢白细胞介素1β 小分子药物

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

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Asian Journal of Andrology 2010年第3期12卷 431-441,I0012页

作者： juan Carlos Martinez-Soto juan de DiosHourcade Alfonso Gutierrez-Adan Jose Lorenzo Landeras Joaquin Gadea Department of Physiology School of Veterinary Murcia University Murcia 30100 Spain IVI- MURCIA Murcia 30007 Spain Department of Animal Reproduction INIA Madrid 28040 Spain

In this study, we evaluated the effects of genistein supplementation of the thawing extender on frozen-thawed human semen parameters. We analyzed the effect of supplementation on sperm motility, capacitation （membrane lipid disorder）, reactive oxygen species （ros） generation, chromatin condensation and DNA damage. Using this preliminary information, it maybe possible to improve the cryopreservation process and reduce the cellular damage. We have confirmed that the isoflavone genistein （10 μmol L-1） has antioxidant properties on the frozen-thawed spermatozoa. This results in a decreased ros production that shows a slight improvement in the sperm motility, and decreases the membrane lipid disorder and DNA damage caused by cryopreservation. These results suggest an effect of genistein on sperm functionality that could be of interest for assisted reproduction treatments using frozen- thawed human spermatozoa, but further studies will be necessary to confirm our findings and to evaluate the possible clinical applications.

关键词： antioxidants cryopreservation DNA damage genistein isoflavones sperm motility

Changes of Reactive Oxygen Species and Related Enzymes in Mitochondrial Respiration During Storage of Harvested Peach Fruits

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Agricultural Sciences in China 2011年第1期10卷 149-158页

作者： KAN juan WANG Hong-mei JIN Chang-hai College of Food Science and Engineering Yangzhou University Yangzhou 225127 P.R.China

Peach fruits [Prumus persica （L.） Batsch, cv. Yuhuasanhao] were used as materials to investigate the changes of reactive oxygen species （ros） and related enzymes in mitochondria respiration during storage and then their influence on senescence of harvested Peach fruits was studied. The results showed that low temperature （5℃） strongly inhibited the reduction of firmness and the increase in respiration rate. During storage at ambient temperature （20℃）, ros had a cumulative process while malondialdehye （MDA） content continued to increase in associated with enhanced membrane lipid peroxidation. Lipoxygenase （LOX） activity was strongly inhibited under the low temperature condition. The activities of succinic dehydrogenase （SDH）, cytochrome C oxidase （CCO）, and Ca^2＋-ATPase declined to a certain extent at ambient temperature, while they showed higher activities at low temperature, which may be related to lower membrane lipid peroxidation at low temperature. Higher Ca^2＋ content at ambient temperature may be responsible for impairment of mitochondrial function, thus, leading to fruit senescence. The results showed that under low temperature condition, the low accumulation of ros and the low level of membrane lipid peroxidation could maintain the function of mitochondria that would help to delay the senescence of peach fruits. These suggested a close relationship existed between ros metabolism and mitochondrial respiration. It can be inferred that the low temperature helps to delay senescence of peach fruits via suppression of ros and related enzymes, maintain better homeostasis of Ca^2＋ in mitochondria and thus better mitochondrial functions.

关键词： peach fruits ripening active oxygen enzymes related to mitochondria respiratory metabolism

Asperuloside Promotes Apoptosis of Cervical Cancer Cells through Endoplasmic Reticulum Stress-Mitochondrial Pathway

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Chinese Journal of Integrative Medicine 2024年第1期30卷 34-41页

作者： QI Zhi-min WANG Xia LIU Xia ZHAO juan Medical Examination Center Hebei Province Hospital of Chinese MedicineShijiazhuang(050011)China Department of Gynaecology and Obstetrics Hebei Province Hospital of Chinese MedicineShijiazhuang(050011)China Experimental Center for Teaching Hebei Medical UniversityShijiazhuang(050017)China

Objective: To investigate the effects of asperuloside on cervical cancer based on endoplasmic reticulum(ER) stress and mitochondrial pathway. Methods: Different doses(12.5–800 μg/mL) of asperuloside were used to treat cervical cancer cell lines Hela and CaSki to calculate the half maximal inhibitory concentration(IC_(50)) of asperuloside. The cell proliferation was analyzed by clone formation assay. Cell apoptosis, intracellular reactive oxygen species(ros) and mitochondrial membrane potential were determined by flow cytometry. The protein expressions of cleaved-caspase-3, Bcl-2, Bax, Cyt-c, cleaved-caspase-4 and glucose-regulated protein 78(GRP78) were analyzed by Western blot. And the inhibitor of ER stress, 4-phenyl butyric acid(4-PBA) was used to treat cervical cancer cells to further verify the role of ER stress in the apoptosis of cervical cancer cells induced by asperuloside. Results: Asperuloside of 325, 650, and 1300 μg/mL significantly inhibited the proliferation and promoted apoptosis of Hela and CaSki cells(Pros levels, reduced mitochondrial membrane potential, significantly reduced Bcl-2 protein expression level, and increased Bax, Cyt-c, GRP78 and cleaved-caspase-4 expressions(P<0.01). In addition, 10 mmol/L 4-PBA treatment significantly promoted cell proliferation and reduced apoptosis(P<0.05), and 650 μg/mL asperuloside could reverse 4-PBA-induced increased cell proliferation, decreased apoptosis and cleaved-caspase-3,-4 and GRP78 protein expressions(P<0.05). Conclusion: Our study revealed the role of asperuloside in cervical cancer, suggesting that asperuloside promotes apoptosis of cervical cancer cells through ER stress-mitochondrial pathway.

关键词： asperuloside Chinese medicine cervical cancer endoplasmic reticulum stress mitochondrial damage apoptosis

Homocysteine activates T cells by enhancing endoplasmic reticulum-mitochondria coupling and increasing mitochondrial respiration

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Protein & Cell 2016年第6期7卷 391-402页

作者： juan Feng Silin Lu Yanhong Ding Ming Zheng Xian Wang Department of Physiology and Pathophysiology School of Basic Medical Science Peking University Key Laboratory of Molecular Cardiovascular Science Ministry of Education Beijing 100191 China

Hyperhomocysteinemia （HHcy） accelerates atherosclero- sis by increasing proliferation and stimulating cytokine secretion in Tcells. However, whether homocysteine （Hcy）- mediated T cell activation is associated with metabolic reprogramming is unclear. Here, our in vivo and in vitro studies showed that Hcy-stimulated splenic T-cell activa- tion in mice was accompanied by increased levels of mitochondrial reactive oxygen species （ros） and calcium, mitochondrial mass and respiration. Inhibiting mitochon- drial ros production and calcium signals or blocking mitochondrial respiration largely blunted Hcy-induced T-cell interferon y （IFN-v） secretion and proliferation. Hcy also enhanced endoplasmic reticulum （ER） stress in T cells, and inhibition of ER stress with 4-phenylbutyric acid blocked Hcy-induced T-cell activation. Mechanistically, Hcy increased ER-mitochondria coupling, and uncou- pling ER-mitochondria by the microtubule inhibitor nocodazole attenuated Hcy-stimulated mitochondrial reprogramming, IFN-y secretion and proliferation in T cells, suggesting that juxtaposition of ER and mitochon-dria is required for Hcy-promoted mitochondrial function and T-cell activation. In conclusion, Hcy promotes T-cell activation by increasing ER-mitochondria coupling and regulating metabolic reprogramming.

关键词： homocysteine T cell mitochondria,endoplasmic reticulum stress

不明原因发热患者的临床特征和实验室检查结果对18F-FDG PET/CT显像诊断效能的影响

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中华核医学与分子影像杂志 2018年第8期38卷 582-583页

作者：唐军 Ana María García-Vicente María Jesús Tello-Galán Mariano Amo-Salas juan ros.izquierdo German Andrés Jiménez-Londofio Beatriz La rosa Salas Guadalupe Prado-Serrano Pradas Francisco José Pena-Pardo ngel Soriano-Castrejón 上海交通大学医学院附属苏州九龙医院核医学科 Nuclear Medicine Department University General HospitalCiudad RealSpain

目的评价患者临床特征及实验室检查结果对18F-脱氧葡萄糖(FDG) PET/CT显像诊断不明原因发热(FUO)的影响。方法回顾性纵向分析FUO患者的PET/CT显像结果。参考标准为血清学检查、各种细菌培养或活组织检查结果,必要时参考其他实验室检查... 详细信息

目的评价患者临床特征及实验室检查结果对18F-脱氧葡萄糖(FDG) PET/CT显像诊断不明原因发热(FUO)的影响。方法回顾性纵向分析FUO患者的PET/CT显像结果。参考标准为血清学检查、各种细菌培养或活组织检查结果,必要时参考其他实验室检查或临床随访结果。收集患者PET/CT显像近几天内的临床特征、炎性标志物、蛋白质分析、血清学检查和细菌培养结果。根据不同病因最终诊断分为3组:第1组为感染或肿瘤,第2组为脉管炎、自身免疫性疾病或非感染性炎性疾病,第3组为未明确诊断的自限性发热或持续性发热。PET/CT显像结果分为阳性或阴性、有助于或无助于诊断发热来源。分析患者临床特征和实验室检查结果对PET/CT结果的影响。结果 67例患者最终诊断分组为:第1组25例,第2组20例,第3组22例。89.6%的患者炎性标志物阳性,28.4%的蛋白质检测阳性,20.9%的细菌培养结果阳性。67例患者中,PET/CT显像阳性52例,25例有助于诊断发热来源,其中对第1组和第2组帮助尤其大。PET/CT显像的灵敏度、特异性和准确性分别为84%、31%和61%。PET/CT显像结果与最终诊断(P=0.035)和细菌培养结果(P=0.037)相关,而与其他临床特征或实验室检查结果无明显相关性。结论18F-FDG PET/CT显像诊断FUO的灵敏度高,但特异性低,可能的原因是FUO的病原范围很广,也有一些是自限性发热。最有可能从PET/CT显像获益的患者是那些多种炎性标志物阳性的患者,这些患者有活动性病变的可能性更高。

关键词：不明原因发热(FUO) 18F-FDG PET/CT 炎性标志物诊断

Hydrogen Peroxide in Plants:a Versatile Molecule of the Reactive Oxygen Species Network

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Journal of Integrative Plant Biology 2008年第1期50卷 2-18页

作者： Li-juan Quan Bo Zhang Wei-Wei Shi Hong-Yu Li MOE Key Laboratory of Arid and Grassland Ecology School of Life Sciences Lanzhou University Lanzhou 730000 China

Plants often face the challenge of severe environmental conditions, which include various biotic and abiotic stresses that exert adverse effects on plant growth and development. During evolution, plants have evolved complex regulatory mechanisms to adapt to various environmental stressors. One of the consequences of stress is an increase in the cellular concentration of reactive oxygen species （ros）, which are subsequently converted to hydrogen peroxide （H2O2）. Even under normal conditions, higher plants produce ros during metabolic processes. Excess concentrations of ros result in oxidative damage to or the apoptotic death of cells. Development of an antioxidant defense system in plants protects them against oxidative stress damage. These ros and, more particularly, H2O2, play versatile roles in normal plant physiological processes and in resistance to stresses. Recently, H2O2 has been regarded as a signaling molecule and regulator of the expression of some genes in cells. This review describes various aspects of H2O2 function, generation and scavenging, gene regulation and cross-links with other physiological molecules during plant growth, development and resistance responses.

关键词： antioxidant system gene regulation hydrogen peroxide (H2O2) reactive oxygen species (ros) signaling molecule.