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Photosynthetic and Molecular Markers of CO_2-mediated Photosynthetic Downregulation in Nodulated Alfalfa

Journal of Integrative Plant Biology 2013年第8期55卷 721-734页

作者： Alvaro Sanz-Sez Gorka Erice Iker Aranjuelo Ricardo Aroca juan manuel Ruíz-Lozano Jone Aguirreolea juan Jos Irigoyen manuel Sanchez-Diaz Department of Plant Biology University of NavarraPamplonaE-31008Spain Department of Plant Biology University of BarcelonaBarcelonaE-8028Spain Department of Plant Biology University of Illinois at Urbana-Champaign61801USA Agrobiotechnology Institute Public University of NavarraMutilva BajaE-31192Spain Department of Soil Microbiology and Symbiotic Systems Zaidin Experimental Station(CSIC)GranadaE-18008.Spain

Elevated CO2 leads to a decrease in potential net photosynthesis in long-term experiments and thus to a reduction in potential growth. This process is known as photosynthetic downregulation. There is no agreement on the definition of which parameters are the most sensitive for detecting CO2 acclimation. In order to investigate the most sensitive photosynthetic and molecular markers of CO2 acclimation, the effects of elevated CO2, and associated elevated temperature were analyzed in alfalfa plants inoculated with different Sinorhizobium meliloti strains. Plants （Medicago sativa L. cv. Aragon） were grown in summer or autumn in temperature gradient greenhouses （TGG）. At the end of the experiment, all plants showed acclimation in both seasons, especially under elevated summer temperatures. This was probably due to the lower nitrogen （N） availability caused by decreased N2-fixation under higher temperatures. Photosynthesis measured at growth CO2 concentration, rubisco in vitro activity and maximum rate of carboxylation were the most sensitive parameters for detecting downregulation. Severe acclimation was also related with decreases in leaf nitrogen content associated with declines in rubisco content （large and small subunits） and activity that resulted in a drop in photosynthesis. Despite the sensitivity of rubisco content as a marker of acclimation, it was not coordinated with gene expression, possibly due to a lag between gene transcription and protein translation.

关键词： Carbon dioxide Medicago sativa (alfalfa) photosynthetic downregulation RbcL and RbcS Sinorhizobium meliloti.

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Building an interoperable space for smart agriculture

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Digital Communications and Networks 2023年第1期9卷 183-193页

作者： Ioanna Roussaki Kevin Doolin Antonio Skarmeta George Routis juan Antonio Lopez-Morales Ethel Claffey manuel Mora juan Antonio Martinez Institute of Communications and Computer Systems AthensGreece Walton Institute WaterfordIreland Department of Information and Communications Engineering University of MurciaMurciaSpain School of Business Waterford Institute of TechnologyWaterfordIreland R&D Department Odin SolutionsMurciaSpain

The digital transformation in agriculture introduces new challenges in terms of data,knowledge and technology adoption due to critical interoperability issues,and also challenges regarding the identification of the most suitable data sources to be exploited and the information models that must be ***(Building an Interoperable,Data-Driven,Innovative and Sustainable European Agri-Food Sector)addresses these challenges by providing an overarching solution that integrates various heterogeneous hardware and software resources(e.g.,devices,networks,platforms)and enables the seamless sharing of data and knowledge throughout the agri-food *** paper introduces the main concepts of DEMETER and its reference architecture to address the data sharing and interoperability needs of farmers,which is validated via two rounds of 20 large-scale pilots along the DEMETER *** paper elaborates on the two pilots carried out in region of Murcia in Spain,which target the arable crops sector and demonstrate the benefits of the deployed DEMETER reference architecture.

关键词： Smart agriculture Internet of things(IoT) DEMETER Reference architecture Interoperability Agricultural information model(AIM) Pilot validation

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Time for a paradigm shift in the classification of muscle injuries

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Journal of Sport and Health Science 2017年第3期6卷 255-261页

作者： Bruce Hamilton juan-manuel Alonso Thomas M.Best High Performance Sport New Zealand Millennium Institute of Sport and HealthMairangi BayAuckland 0110New Zealand Sports Medicine Department Qatar Orthopaedic and Sports Medicine HospitalDoha 22922Qatar UHealth Sports Medicine Institute University of Miami Miller School of MedicineMiamiFL 33136USA

Muscle injuries remain one of the most common injuries in sport,yet despite this,there is little consensus on how to either effectively describe or determine the prognosis of a specific muscle *** approaches to muscle classification and grading of medicine have been applied over the last century,but over the last decade the limitations of historic approaches have been *** a consequence,in the past 10 years,clinical research groups have begun to question the historic approaches and reconsider the way muscle injuries are classified and *** a narrative approach,this manuscript describes several of the most recent attempts to classify and grade muscle injuries and highlights the relative strengths and weaknesses of each *** each of the new classification and grading systems have strengths,there remains little consensus on a system that is both comprehensive and evidence *** of the currently identified features within the grading systems have relevance to accurately determining prognosis.

关键词： Classification Grading Magnetic resonance imaging Muscle injury Return to play Ultrasound

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Role of endoscopic ultrasound in the diagnosis of pancreatic cancer

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World Journal of Gastrointestinal Oncology 2014年第9期6卷 360-368页

作者： juana Gonzalo-Marin juan Jose Vila manuel Perez-Miranda Unit of Endoscopy Department of GastroenterologyQuirón Hospital Unit of Endoscopy Department of GastroenterologyComplejo Hospitalario de Navarra Department of Gastroenterology Hospital Universitario Rio Hortega

Endoscopic ultrasonography(EUS) with or without fine needle aspiration has become the main technique for evaluating pancreatobiliary disorders and has proved to have a higher diagnostic yield than positron emission tomography,computed tomography(CT) and transabdominal ultrasound for recognising early pancreatic *** a diagnostic modality for pancreatic cancer,EUS has proved rates higher than 90%,especially for lesions less than 2-3 cm in size in which it reaches a sensitivity rate of 99% vs 55% for ***,EUS has a very high negative predictive value and thus EUS can reliably exclude pancreatic *** complication rate of EUS is as low as 1.1%-3.0%.New technical developments such as elastography and the use of contrast agents have recently been applied to EUS,improving its diagnostic *** has been found to be superior to the recent multidetector CT for T stagingwith less risk of overstaying in comparison to both CT and magnetic resonance imaging,so that patients are not being ruled out of a potentially beneficial *** accuracy for N staging with EUS is 64%-82%.In unresectable cancers,EUS also plays a therapeutic role by means of treating oncological pain through celiac plexus block,biliary drainage in obstructive jaundice in patients where endoscopic retrograde cholangiopancreatography is not affordable and aiding radiotherapy and chemotherapy.

关键词： Endosonography Pancreatic neoplasms Endoscopy Diagnosis Neoplasm Staging Therapeutics

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Comorbidity negatively influences prognosis in patients with extrahepatic cholangiocarcinoma

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World Journal of Gastroenterology 2009年第42期15卷 5279-5286页

作者： Mario Fernández-Ruiz juan-manuel Guerra-Vales Francisco Colina-Ruizdelgado Department of Internal Medicine University Hospital "12 de Octubre"Avenue Córdobas/nUniversidad ComplutenseMadrid 28041Spain Hospital Tumor Registry University Hospital "12 de Octubre"Avenue Córdobas/nUniversidad ComplutenseMadrid 28041Spain

AIM:To study the outcome and prognostic factors in a series of patients with extrahepatic cholangiocarcinoma and determine the impact of comorbidity on ***:A retrospective analysis of 68 patients with extrahepatic cholangiocarcinoma(perihilar,n = 37;distal,n = 31) seen at a single tertiary-care institution during the period 1999-2003 was *** on presentation,management,and outcome were assessed by chart *** confirmation was obtained in 37 cases(54.4%) .Comorbidity was evaluated by using the Charlson comorbidity index(CCI) .RESULTS:Mean age at diagnosis was 73.4 ± 11.5 *** was the most common symptom presented(86.8%) .Median CCI score was 1(range,0 to 4) .Nineteen patients(27.9%) underwent tumor *** biliary drainage was performed in 39 patients(57.4%) ,and 6 patients(8.8%) received only best supportive ***-free margin status(R0) was achieved in 15 cases(78.9% of resection group) .Baseline serum carbohydrate antigen 19-9(CA 19-9) level was revealed to be an independent predictor of surgical treatment(P = 0.026) .Overall median survival was 3.1 ± 0.9 mo,with 1-and 2-year survival rates of 21% and 7%,*** the univariate analysis,tumor resection,CCI score,and serum CA 19-9 levels correlated significantly with *** the multivariate analysis,only resection(HR 0.10;95% CI,0.02-0.51,P = 0.005) and a CCI score ≥ 2(HR 3.36;95% CI,1.0-10.9,P = 0.045) were found to independently predict ***:Tumor resection and comorbidity emerged as significant prognostic variables in extrahepatic *** evaluation instruments should be applied in the clinical management of such patients.

关键词： Charlson index Cholangiocarcinoma Comorbidity Prognosis Survival

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Linking the hierarchical decision- making process of fruit choice and the phenotypic selection strength on fruit traits by birds

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Journal of Plant Ecology 2017年第4期10卷 713-720页

作者： Facundo X.Palacio juan manuel Girini Mariano Ordano Fundación Miguel Lillo Miguel Lillo 251CP T4000JFESan Miguel de TucumánTucumánArgentina Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET) Argentina Sección Ornitología División Zoología VertebradosFacultad de Ciencias Naturales y MuseoUniversidad Nacional de La PlataPaseo del Bosque s/nCP B1900FWALa PlataBuenos AiresArgentina

Aims Animals in search of fleshy fruits forage mostly according to the number of available fruits and then select individual fruits based on reward quality or advertised subtle *** hierarchical pat-tern of fruit choice would be translated into patterns of selection strength mediated by frugivores on fruit display ***,frugi-vores would exert higher selection pressures on fruit crop size and lower selection pressures on within-plant variation of phenotypic traits(infructescence,fruit and seed size).However,no attempt to link this behavioral mechanism of hierarchical trait selection to natural selection patterns has been ***,we sought to determine the relationship between the hierarchical decision-mak-ing process of fruit choice and patterns of natural selection on fruit *** We recorded bird visits and measured fruit-related traits(fruit crop size,fruit diameter and seed weight)in a natural population of Psychotria carthagenensis,a bird-dispersed treelet,in a Yungas for-est from *** assess phenotypic selection patterns on fruit display traits,we performed multivariate selection analysis,and to explicitly identify a hierarchy of fruit trait choice we used a classifi-cation tree as a predictive *** Findings Selection patterns on fruit display traits were in agreement with a hierarchical process of fruit choice made by *** strength of directional selection on the total number of fruits in a plant(*** crop size)was nearly two times higher than on fruit size,and the classification tree analysis supported this hierarchical *** results support previous evidence that seed dispersers shape fruit crop size with higher intensity than subindividual fruit ***,high levels of subindividual phenotypic variation of fruit display traits may be explained by relaxed selection pressures exerted by *** studies also show that this pattern may consti-tute a general phenomenon among other pl

关键词： plant-animal interactions seed dispersal selection gradients subindividual variation

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Effects of maternal dietary omega-3 polyunsaturated fatty acids and methionine during late gestation on fetal growth,DNA methylation,and mRNA relative expression of genes associated with the inflammatory response,lipid metabolism and DNA methylation in placenta and offspring’s liver in sheep

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Journal of Animal Science and Biotechnology 2021年第2期12卷 611-625页

作者： Milca Rosa Velazquez Fernanda Batistel juan manuel Pinos Rodriguez Alejandro Enrique Relling Facultad de Medicina Veterinaria y Zootecnia Universidad Veracruzana91710 VeracruzMexico Department of Animal Science Ohio Agricultural Research and Development Center(OARDC)The Ohio State University114 Gerlaugh Hall1680 Madison AveWoosterOH 44691USA Department of Animal Dairy and Veterinary SciencesUtah State UniversityLoganUT 84322USA

Background:Omega-3 PUFA or methionine(Met)supply during gestation alters offspring ***,the effect of both nutrients on fetal development has not been *** objective was to determine the effects of supplementation of these two nutrients during late gestation on fetal growth,DNA methylation,and mRNA expression of genes associated with the inflammatory response,and DNA ***(n=5/treatment)were fed from day 100 to 145 of gestation one of the following treatments:1)basal diet(NS)without fatty acids(FS)or methionine(MS)supplementation;2)FS(10 g/kg Ca salts,source omega-3 PUFA);3)MS(1 g/kg rumen protected methionine);and 4)FS and MS(FS-MS).On day 145,ewes were euthanized,and data from dams and fetus was ***(cotyledon),fetal liver,and blood samples were ***:A treatments interaction on fetal liver weight,ewe body weight and body condition score(BCS)was observed;FS-MS were heavier(P<0.01)than FS and MS,and FS-MS ewes had a better(P=0.02)BCS than *** increased(P=0.03)ewe plasma glucose *** liver global DNA methylation increased(P<0.01)in FS and *** treatments modify the mRNA relative expression on some of the genes *** the fetal liver,FS increased(P=0.04)the mRNA relative expression of arachidonate-5-lipoxygenase-activatingprotein and tended to decrease(P=0.06)***,MS decreased(P=0.04)DNA-methyltransferase-1 and tended to decrease(P=0.08)free-fatty-acid-receptor-1 mRNA relative ***,FS-MS decreased mRNA relative expression of tumor-necrosis-factor-alpha(P=0.05),peroxisomeproliferator-activated-receptor-delta(P=0.03)and gamma(P=0.04),tended to decrease(P≤0.09)interleukin-6,fatty-acid-transport-protein-1,and delta-5-desaturase,and increased adenosylhomocysteinase(P=0.04)mRNA relative *** cotyledon,FS tended to decrease fatty acid binding protein 4(P=0.09)mRNA relative ***:Omega-3 PUFA and Met s

关键词： Fat supplementation Fetal programming Maternal nutrition Nutrients Rumen protected methionine

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Intelligent collision risk detection in medium-sized cities of developing countries,using naturalistic driving:A review

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Journal of Traffic and Transportation Engineering(English Edition) 2022年第6期9卷 912-929页

作者： juan Jose Paredes Santiago Felipe Yepes Ricardo Salazar-Cabrera Alvaro Pachon dela Cruz juan manuel Madrid Molina Telematics Engineering Research Group(GIT) Telematics DepartmentUniversidad Del CaucaPopayanColombia Information Technology and Telecommunications Research Group(12T) Universidad IcesiCaliColombia

Traffic accidents are one of the most serious problems worldwide,being one of the leading causes of death and economic loss in the ***-and middle-income countries,mainly their medium-sized cities,are among the most affected by this problem.93%of traffic accidents occur in low and middle-income countries,even though these countries have approximately 60%of the world’s *** occurs mainly because in these types of countries,especially in medium-sized cities(target context),there are no ideal conditions for driving,such as adequate road infrastructure,good condition of vehicles,and rigorous safety *** data analysis techniques including machine learning(ML)have increasingly been used to solve this *** driving(ND)can be applied as a data collection method that provides information on traffic *** commonly uses a vehicle’s kinematic data to detect high-risk driving behaviors that could cause an *** objectives of this document are to present a review of different alternatives that help in data collection and creation of intelligent solutions related to detection of possible traffic accidents,principally using ND;and to propose an intelligent collision risk detection system(ICRDS)for identification of areas with a high probability of TA in the target *** the review,it was possible to analyze and evaluate the devices,variables and algorithms that help characterize a risk event in driving,considering the target *** development of a prototype of an ICRDS for a medium-sized city in a developing country is considered viable,considering the identified components,with the aim of identifying risk events in driving,and areas of high probability of accidents in the city.

关键词： Naturalistic driving Near-crash Traffic accident Vehicle data collection

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Sustainable transit vehicle tracking service,using intelligent transportation system services and emerging communication technologies:A review

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Journal of Traffic and Transportation Engineering(English Edition) 2020年第6期7卷 729-747页

作者： Ricardo Salazar-Cabrera álvaro Pachón de la Cruz juan manuel Madrid Molina Telematics Engineering Research Group(GIT) Telematics DepartmentUniversidad del CaucaPopayanColombia Information Technology and Telecommunications Research Group(I2T) ICT DepartmentUniuersidad IcesiCaliColombia

The most salient problems of transit vehicle service in Latin American intermediate cities include:the high number of passengers involved in traffic accidents;traffic congestion caused by transit vehicles,and pollution generated by these vehicles,which increases in high congestion *** improve upon this situation,a research was conducted on the transit vehicle tracking service,which is a basic service for implementing mobility solutions for the aforementioned problems,the most relevant characteristics of this service for the context of Latin American intermediate cities were identified,and an implementation was *** paper presents the four stages of the study:(a)a review of the state-of-the-art of services or systems related to vehicle tracking,including wireless communications technologies,implemented sustainability approaches,usage of special algorithms for efficiency improvement,and the intelligent transportation system(ITS)architecture used as a basis;(b)the process of identifying relevant characteristics of the service for a given context;(c)proposal of an ITS architecture for this service in an intermediate city,its requirements and the suggested technologies;and(d)development of experiments for validating usage of the key suggested *** review allowed to identify the main service characteristics,with regard to vehicle positioning technologies,the recommended wireless communication technology(long range,LoRa),energy consumption considerations,and use of artificial intelligence(AI)to calculate waiting time of users at bus ***,an ITS architecture for the city of Popayan(Colombian city)considering the aforementioned characteristics is proposed,and the experiments related to the use of these technologies are described in detail.

关键词： Transportation engineering Intelligent transportation systems Transit vehicle ITS architecture Tracking Long range

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Functional foods based on the recovery of bioactive ingredients from food and algae by-products by emerging extraction technologies and 3D printing

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Food Bioscience 2022年第5期49卷 1090-1102页

作者： Pauline Donn Miguel A.prieto juan C.Mejuto Hui Cao Jesus Simal-Gandara University of Yaounde 1 P.O.BOX 337YaoundeCameroon Universidade de Vigo Nutrition and Bromatology GroupDepartment of Analytical Chemistry and Food ScienceFaculty of ScienceE32004OurenseSpain Universidade de Vigo Department of Physical ChemistryFaculty of ScienceE32004OurenseSpain

3D food printing is an emerging technology developed to facilitate the life of consumers and food *** technology allows to obtain any type of new foods according to our *** is possible to develop a food with the exact nutritive value necessary for our body,with the most benefiting nutrients we want,or without any ingredients that we have an allergy,and even predict or personalize the taste,the color,the shape,and the size of a ***,3D food printing is considered a promising strategy for developing healthy *** the other hand,many foods enterprises release high amounts of waste from their processing *** wastes contain many bioactive ingredients such as polyphenols,carotenoids,vitamins,minerals,fibers,unsaturated fatty acids,among others,which have physiological and health ***,several bioactive compounds have been identified in *** can be extracted by conventional methods with solvents such as water,ethanol,methanol,chloroform,acetone,and many others,but with some limits like environmental contamination,human toxicity,and low extraction *** these reasons,it will be interesting to use emerging extraction technologies to recover bioactive compounds and use them in a 3D food printer to make functional foods that can bring a targeted health benefit to consumers.

关键词： bioactive methanol exact

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