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Antifungal Potential of Beauveria bassiana on Solanum lycopersicum L. Infected with Fusarium oxysporum f. sp. lycopersici

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Phyton-International Journal of Experimental Botany 2023年第4期92卷 1235-1255页

作者： Henry López-López Nancy Ruiz-Lau Rocío Meza-Gordillo Víctor Manuel Ruiz-Valdiviezo joseph Galthier Robledo-Luchetti Carlos Alberto Lecona-Guzmán juan josévillalobos-maldonado Luc Dendooven Joaquín Adolfo Montes-Molina Laboratorio de Biotecnología vegetal Tecnológico Nacional de Mexico/Campus Tuxtla GutiérrezTuxtla Gutiérrez29050Mexico CONACYT-Tecnológico Nacional de Mexico/Campus Tuxtla Gutiérrez Tuxtla Gutiérrez29050México ABACUS CinvestavCiudad de México07360México

The objective of this work was to evaluate the effect of Beauveria bassiana(Bb 1205)on controlling Fusarium oxysporum ***(Fol 17108)in tomato plants in greenhouse *** of Bb 1205 was the most promising among the agronomic variables and expression of the activity of the enzymesβ-1,3-glucanases and *** of Bb 1205 occurred at a concentration of 1×108 conidia·mL−1,which was administered onto the leaves,directly into the soil and via *** with Fol 17108 occurred with 1×106 spores·mL−1,which were added directly to the *** was used for measuring agronomic parameters,namely activity of chitinases andβ-1,3-glucanases in foliage and *** Bb 1205 was added to the soil,the chlorophyll index and aerial part length showed significant *** addition,it was determined that root length,fresh weight of foliage,flower,and fruit count increased 82 days after inoculation(dai).Chitinase activity induced by Bb 1205 in leaves and roots of tomato plants infected with Fol 17108 was observed when injected into the stem at 32 dai(41.8 and 11.6-fold,respectively).Inoculation on the foliage showed a 10-fold increase ofβ-1,3-glucanases in the roots after 82 *** for leaves,a 3.8-fold increase was found when the stem was *** the different in vivo applications,Bb 1205 activated its defenses by expressing the chitinase enzymes andβ-1,3-glucanase,thus reducing the damage caused by Fol 17108,demonstrating increase plant growth thereafter.

关键词： Mycoparasitism agronomic parameters chitinases β-1,3-glucanases

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Comparison of Apigenin,Quercetin and Kaempferol Accumulation and Total Flavonoid Content in Leaves,Embryogenic Cultures and Cell Suspension Cultures of Parsley(Petroselinum crispum)

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Phyton-International Journal of Experimental Botany 2023年第10期92卷 2807-2823页

作者： Laura Isabel Arias-Rodríguez Martha Alicia Rodríguez-Mendiola Carlos Arias-Castro Federico Antonio Gutiérrez Miceli Diana Reséndez Pérez María Celina Luján Hidalgo juan josévillalobos maldonado Norma Alejandra Mancilla Margalli Analytical Laboratory National Technological Institute of MexicoTuxtla Gutierrez CampusTuxtla GutierrezMexico Plant Biotechnology Laboratory Instrumental Analysis LaboratoryPlant Biochemistry LaboratoryNational Technological Institute of MexicoTlajomulco de ZunigaMexico Department of Cellular Biology and Genetics Faculty of Biological SciencesAutonomous University of Nuevo LeonSan Nicolas delos GarzaMexico

In recent years,there has been a growing interest in exploring alternative treatments for bone *** tissue engineering has turned its attention to plant extracts containing osteogenic flavonoids as potential promoters of bone *** our study,we specifically investigated the extract of Petroselinum crispum,a plant known for its abundance of osteogenic flavonoids such as apigenin,quercetin,and *** objective was to compare the total flavonoid content(TFC)and their accumulation in different *** obtained hydrolyzed aqueous extracts from the leaves of parsley plants(grown for 12 weeks in the field),weekly embryogenic cultures,and suspension cell *** TFC,measured in mg quercetin equivalent per gram of dry weight(QE mg/g dw),was found to be 53.81±0.97 for 52 g dw of leaves(1 kg plant fw),31.10±1.52 for 420 g dw of embryogenic cultures,and 11.80±0.76 for 210 g dw of cell ***,only the leaves and embryogenic cultures showed significant accumulation of osteogenic flavonoids,with apigenin levels of 1.8±0.3 and 0.32±0.02 mg/g dw,and kaempferol levels of 1.0±0.18 and 0.2±0.01 mg/g dw,*** findings indicate that embryogenic cultures have the potential to produce up to 40%more apigenin in 12 weeks compared to 1 kg of parsley plant,thanks to their superior biomass reproductive *** results present a promising biotechnological approach for generating extracts enriched with bioactive compounds,which can be further utilized in in vitro osteogenic testing.

关键词： Parsley(Petroselinum crispum)cultures osteogenic flavonoids bone regeneration aqueous extracts

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Post-acute pancreatitis diabetes:A complication waiting for more recognition and understanding

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World Journal of Gastroenterology 2023年第28期29卷 4405-4415页

作者： Diego García-Compeán Alan R Jiménez-Rodríguez juan M Muñoz-Ayala josé A González-González Héctor J maldonado-Garza Jesús Z Villarreal-Pérez Department of Gastroenterology Autonomous University of Nuevo LeónMonterrey 64700Nuevo LeónMexico Department of Endocrinology Autonomous University of Nuevo LeónMonterrey 64700Nuevo LeónMexico

Post-acute pancreatitis diabetes(PAPD)is the second most common type of diabetes below type 2 diabetes *** to the boom in research on this entity carried out during the last decade,its recognition has ***,much of the medical community still does not recognize it as a medium and long-term complication of acute pancreatitis(AP).Recent prospective cohort studies show that its incidence is about 23%globally and 34.5%in patients with severe *** the overall increase in the incidence of AP this complication will be certainly seen more *** to its high morbidity,mortality and difficult control,early detection and treatment are ***,its risk factors and pathophysiological mechanisms are not clearly *** diagnosis should be made excluding pre-existing diabetes and applying the criteria of the American Diabetes Association after 90 d of resolution of one or more AP *** review will show the evidence published so far on the incidence and prevalence,risk factors,possible pathophysiological mechanisms,clinical outcomes,clinical characteristics and preventive and corrective management of *** important gaps needing to be clarified in forthcoming studies will also be discussed.

关键词： Acute pancreatitis Diabetes mellitus Chronic pancreatitis Post-pancreatitis diabetes Pancreatogenic diabetes

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Secondary Metabolites of Entomopathogenic Fungi, Biological Alternative for the Control of Agricultural Pests and Disease: Present and Perspectives

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Advances in Enzyme Research 2024年第1期12卷 1-15页

作者： Nallely San juan-maldonado Silvia Rodríguez-Navarro Alejandro Angel-Cuapio josé Norberto Vásquez Bonilla juan Esteban Barranco-Florido Departamento de Producció n Agr&#237cola y Animal Universidad Aut&#243noma Metropolitana Unidad Xochimilco Ciudad de M&#233xico M&#233xico Divisió n de Ingenier&#237a Qu&#237mica y Bioqu&#237mica Tecnol&#243gico Nacional de M&#233xico/TES de Ecatepec Ecatepec de Morelos M&#233xico Departamento de Sistemas Bioló gicos Universidad Aut&#243noma Metropolitana Unidad Xochimilco Ciudad de M&#233xico M&#233xico

The use of entomopathogenic fungi (EF) in recent years has been highly effective against the different orders of insects considered pests of agricultural importance and their conidia have been commonly applied, but it has been reported that these are sensitive to the environmental conditions. For this reason, biopesticides products have been formulated based on secondary metabolites, recently. These biomolecules participate as biological control agent, such as: cyclic depsipeptides, amino acids, polyketides, polyphenols and terpenoids, affecting their morphology, life cycle and insect behavior. The use of secondary metabolites of entomopathogenic fungi opens the possibility of application in a more efficient way for the control of agricultural pests in a compatible with the environment and human health;therefore, it is important to know, analyzing the type of molecules, their effects, and their different methods of application.

关键词： Secondary Metabolites Entomopathogenic Fungi Biological Control Agent

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Dealing with a hyper-successful neighbor: effects of the invasive apple snail Pomacea canaliculata on exotic and native snails in South America

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Current Zoology 2019年第3期65卷 225-235页

作者： Mara A.maldonado Pablo R.MARTIN Laboratorio de Ecologia (DBByF) INBIOSUR (Universidad Nacional del Sur-CONICET) San Juan 670 Bahia Blanca(8000) Argentina

Pomacea canaliculata is a successful invader and also a competitor and predator of other snails and may play a key role in structuring freshwater snail communities both in its native and invaded range. In the present study we evaluated the contact and distant effects of P. canaliculata in its native range on exotic (Melanoides tuberculata and Physa acuta) and native snails (Heleobia parchappii, Biomphalaria peregrina, and Chilina parchappii). Habitat use was affected in P. acuta, H. parchappii, and B. peregrina by contact effects of P. canaliculata, whereas survival was only affected in P. acuta through combined con tact and dista nt effects. Fee undity was reduced in P. acuta and B. peregrina by combined con tact and distant effects;evidence of egg mass predation was also observed in both species. Melanoides tuberculata was not affected at all by P. canaliculata. The snail species with higher withdrawal resp on ses to con tacts with P. canaliculata were those that suffered less mortality by corporal contact, whereas snails with high crawling away responses suffered from higher mortality. The effects of P. canaliculata seem to be highly negative to small non operculate sn ails that lay gelati nous egg masses, whereas large operculate ovoviviparous snails are not affected in their survival and reproducti on. This apple snail may exert biotic resista nee agai nst P. acuta but could favor the establishme nt of M. tuberculata and other function ally similar species in new habitats in South America.

关键词： Ampullariidae behavior biotic resistance interspecific interactions

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Experimental and Numerical Studies of Paraguayan Chaco Natural Gas in a Counterflow Diffusion Flame

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Journal of Mathematics and System Science 2015年第9期5卷 369-375页

作者： Hemando maldonado Dario Alviso Miguel Mendieta juan Carlos Rolon Facultad de Ingenieria Universidad Nacional de Asuncion San Lorenzo 2160 Paraguay.

Combustion is a chemical phenomenon in which a multitude of elementary chemical reactions take place, resulting in the overall process of fuel oxidation. Natural gas fuel has been explored for a few decades and extracted for a few years in the region of Paraguayan Chaco, near Bolivia border. Currently, natural gas is not very important in Paraguay＇s energy matrix, however it could be in the near future if higher volumes are extracted and transported to the most populated cities, specially to the capital. In order to improve Paraguayan natural gas combustion performance, an understanding of its fundamental properties and the combustion pathways is required. This study presents new data for Paraguayan Chaco natural gas combustion in a laminar counterflow diffusion flame configuration at atmospheric pressure. Visible chemiluminescence of excited radicals CH＊ and C2^＊ is employed experimentally. 1D numerical simulation was carried out using Paraguayan Chaco natural gas chemical composition and a standard kinetic mechanism, to which we added CH＊ and C] reactions. Typical flame structures resulting from simulation are presented and a validation of the model is realized comparing experimental and numerical CH＊ and C~ radicals profiles.

关键词： Natural gas counterflow diffusion flames numerical simulation kinetic modeling chemiluminescence

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Effect of L-carnitine supplementation during in vitro maturation and in vitro culture on oocyte quality and embryonic development rate of bovines

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Asian pacific Journal of Reproduction 2019年第6期8卷 289-296页

作者： Diego F.Carrillo-González Nélida Rodríguez-Osorio Charles R.Long Neil A.Vásquez-Araque juan G.maldonado-Estrada One Health and Veterinary Innovative Research and Development Group School of Veterinary MedicineUniversity of Antioquia050034 MedellinColombia Faculty of Agricultural Sciences School of ZootechnicsUniversidad de SucreColombia Genomics and Bioinformatics Lab Department of Biological SciencesUniversity of the Republic of UruguaySalto CampusUruguay Department of Veterinary Physiology and Pharmacology College of Veterinary MedicineTexas A&M UniversityCollege StationTexas 77843-4466USA Grupo de investigación Biotecnología Animal Facultad de CienciasUniversidad Nacional de ColombiaSede MedellínMedellínColombia

Objective:To assess the effect of L-carnitine supplementation during in vitro oocyte maturation and in vitro culture process of bovine ***:L-carnitine(3.8 mM)was added to maturation medium and the effect was assessed in the quality(Experiment 1)and in the cleavage and 4-cells stage(Experiment 2).Besides,the effect of L-carnitine addition on maturation medium(3.8 mM)and culture medium(1.5 mM)on embryo rate production was *** Experiment 1,bovine oocytes from abattoir were randomly separated into two groups(the control group and L-carnitine group)forin vitro *** oocytes were examined for cumulus cells expansion as an indicator of maturation,and the content of the mitochondrial activity,the presence of lipid droplets,the reduced glutathione,and the reactive oxygen species were measured by using specific *** Experiment 2,oocytes were matured as performed in Experiment 1,afterward fertilized and cultured until day 3,and cleavage rate and 4-cells stage rate were *** Experiment 3,in vitro maturation and fertilization were done as performed in Experiment 2,but at day 3 of culture,each group of embryos was separated into two new groups,and L-carnitine(1.5 mM)was added in culture media until day *** cleavage and embryo development rate were determined on the basis with the oocytes put on *** rate was calculated from cleaved ***:The cumulus expansion rate at gradeⅢand mitochondrial activity were significantly higher in the L-carnitine group in comparison with the control group(P0.05).In addition,cleavage and the proportion of embryo development and hatching rate were similar for all groups(P>0.05).Conclusions:L-carnitine as a supplement in culture media improves the cumulus expansion and increases the mitochondrial activity during in vitro maturation process but has no apparent effect on the cleavage and development of bovine *** investigations of L-carnitine addition on in vit

关键词： Antioxidants Culture supplement Embryo development Lipid droplets Maturation Mitochondria

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Zeolite-templated carbons as effective sorbents to remove methylsiloxanes and derivatives:A computational screening

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Green Energy & Environment 2021年第6期6卷 884-892页

作者： Shiru Lin Kaitlyn A.Jacoby Jinxing Gu Dariana R.Vega-Santander Arturo J.Hernaandez-maldonado Zhongfang Chen Department of Chemistry University of Puerto RicoRío PiedrasSan JuanPR 00931USA Department of Chemistry&Biochemistry Elizabethtown CollegeElizabethtownPA 17022USA Department of Chemical Engineering University of Puerto RicoMayaguez CampusMayaguezPR 00681USA

Though widely used in our daily lives,volatile methylsiloxanes and derivatives are emerging contaminants and becoming a high-priority environment and public health *** effective sorbent materials can remove siloxanes in a cost-effective ***,by means of Grand Canonical Monte Carlo(GCMC)simulations,we evaluated the potentials of the recently proposed 68 stable zeolite-templated carbons(ZTCs)(PNAS 2018,115,E8116-E8124)for the removal of four linear methylsiloxanes and derivatives as well as two cyclic methylsiloxanes by the calculated average loading and average adsorption energy *** ZTCs,namely ISV,FAU1,FAU3,and H8326836,were identified with the top 50%adsorption performance toward all the six targeted contaminants,which outperform activated *** first principles computations revealed that steric hindrance,electrostatic interactions(further enhanced by charge transfer),and CH-p interactions account for the outstanding adsorption performance of these *** work provides a quick procedure to computationally screen promising ZTCs for siloxane removal,and help guide future experimental and theoretical investigations.

关键词： Zeolite-templated carbons Contaminants of emerging concern Methylsiloxanes GCMC simulations Density functional theory calculations

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Oral allopurinol to prevent hyperamylasemia and acute pancreatitis after endoscopic retrograde cholangiopancreatography

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World Journal of Gastroenterology 2009年第13期15卷 1600-1606页

作者： Hector Martinez-Torres Xochilt Rodriguez-Lomeli Carlo Davalos-Cobian Jesus Garcia-Correa juan Manue maldonado-Martinez Fabiola Medrano-Muoz Clotilde Fuentes-Orozco Alejandr Gonzalez-Ojeda Department of Gastroenterology an Endoscopy UMAE-Hospital de Especialidades del Centr Medico Nacional de Occidente-IMSS Research Unit in Clinical Epidemiology UMAE-Hospital de Especialidades del Centro Medico Naciona de Occidente-IMSS

AIM:To assess the efficacy of allopurinol to prevent hyperamylasemia and pancreatitis after endoscopic retrograde cholangiopancreatography(PEP).METHODS:One hundred and seventy patients were enrolled and randomized to two groups:a study group(n=85)who received 300 mg of oral allopurinol at 15 h and 3 h before endoscopic retrograde cholangiopancreatography(ERCP)and a control group(n=85)receiving an oral placebo at the same *** Outcome Measurements included serum amylase levels and the number severity of the episodes of *** amylase levels were classified as normal(151 IU/L).Episodes of PEP were classified following Ranson's criteria and CT severity ***:Gender distribution was similar between *** age was 53.5±18.9 years for study group and 52.8±19.8 years for ***,the distribution of benign pathology was similar between *** was more common in the control group(P=0.003).Mild PEP developed in two patients from the study group(2.3%)and eight(9.4%) from control group(P=0.04),seven episodes were observed in high-risk patients of the control group(25%) and one in the allopurinol group(3.3%,P=0.02).Risk factors for PEP were precut sphincterotomy(P=0.02),pancreatic duct manipulation(P=0.002)and multiple procedures(P=0.000).There were no deaths or side ***:Oral allopurinol before ERCP decreased the incidences of hyperamylasemia and pancreatitis in patients submitted to high-risk procedures.

关键词： Endoscopic retrograde cholangiopancreatography Hyperamylasemia Acute pancreatitis Oralallopurinol Risk factors

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Diagnosis and treatment of hepatocellular carcinoma: An update

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World Journal of Hepatology 2015年第3期7卷 362-376页

作者： Javier Tejeda-maldonado Ignacio García-Juárez Jonathan Aguirre-Valadez Adrián González-Aguirre Mario Vilatobá-Chapa Alejandra Armengol-Alonso Francisco Escobar-Penagos Aldo Torre juan Francisco Sánchez-ávila Diego Luis Carrillo-Pérez Department of Internal Medicine Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán Department of Gastroenterology Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán Department of Interventional Radiology Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán Department of Transplantation Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán Department of Hematology and Oncology Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

Hepatocellular carcinoma(HCC) is one of the most common malignancies leading to high mortality rates in the general population; in cirrhotic patients, it is the primary cause of death. The diagnosis is usually delayed in spite of at-risk population screening recommendations, i.e., patients infected with hepatitis B or C virus. Hepatocarcinogenesis hinges on a great number of genetic and molecular abnormalities that lead to tumor angiogenesis and foster their dissemination potential. The diagnosis is mainly based on imaging studies such as computed tomography and magnetic resonance, in which lesions present a characteristic classical pattern of early arterial enhancement followed by contrast medium "washout" in late venous phase. On occasion, when imaging studies are not conclusive, biopsy of the lesion must be performed to establish the diagnosis. The Barcelona Clinic Liver Cancer staging method is the most frequently used worldwide and recommended by the international guidelines of HCC management. Currently available treatments include tumor resection, liver transplant, sorafenib and locoregional therapies(alcoholization, radiofrequency ablation, chemoembolization). The prognosis of hepatocarcinoma is determined according to the lesion's stage and in cirrhotic patients, on residual liver function. Curative treatments, such as liver transplant, are sought in patients diagnosed in early stages; patients in more advanced stages, were not greatly benefitted by chemotherapy in terms of survival until the advent of target molecules such as sorafenib.

关键词： Hepatocellular carcinoma Surveillance Liver transplant Sorafenib Catheter ablation

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