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BIOCEll 2022年第12期46卷 2615-2624页

作者： HAIFENG WANG juan GUO TIEQIAO WEN School of Environmental Engineering Central Plains Specialty Food Engineering&Technology Research CenterKaifeng Key Laboratory of Food Composition and Quality AssessmentYellow River Conservancy Technical InstituteKaifeng475004China laboratory of Molecular Neural Biology School of Life SciencesShanghai UniversityShanghai200444China

The characterization and functions of transmembrane protein 59-like(TMEM59l),a type I transmembrane protein,are not clearly understood until *** TMEM59l and fluorescent fusion proteins constructs were transfected in cell lines and liposomes,and their localization was *** effects of protein constructs were studied by fluorescence microscopy and western *** study reports a novel function of human TMEM59l(hTMEM59l)related to the expression and location of some *** addition,we report two novel splice variants of human TMEM59l(hTMEM59l).The localization of mutants of this protein,lacking a middle region,and a Cterminal deletion,markedly differed from that of full-length *** movement assessment in living cells showed the localization of TMEM59l to vesicular structures in the Golgi bodies,and the cell membrane was observed in living *** of TMEM59l markedly increased the level of amyloid precursor protein because of TMEM59l-mediated inhibition of cell membrane transport but did not affect the expression of betasecretase ***59l overexpression also significantly increased the levels of Rab GDP dissociation inhibitor alpha and Rab GDP dissociation inhibitor beta *** results suggest that TMEM59l is involved in the packaging of acidic vesicles and thereby functions in the trafficking and processing of intracellular proteins.

关键词： TMEM59l localization GDI1 GDI2 Protein processing

Block Compressed Sensing Image Reconstruction Based on Sl0 Algorithm

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Journal of Beijing Institute of Technology 2017年第3期26卷 357-366页

作者： juan Zhao Xia Bai Jieqiong Xiao School of Information and Electronics Beijing Institute of TechnologyBeijing 100081China Beijing Key laboratory of Fractional Signals and Systems Beijing 100081China)

By applying smoothed l0norm（Sl0）algorithm,a block compressive sensing（BCS）algorithm called BCS-Sl0 is proposed,which deploys Sl0 and smoothing filter for image ***,BCS-ReSl0 algorithm is developed to use regularized Sl0（ReSl0）in a reconstruction process to deal with noisy *** study shows that the proposed BCS-Sl0 takes less execution time than the classical BCS with smoothed projected landweber（BCS-SPl）algorithm in low measurement ratio,while achieving comparable reconstruction quality,and improving the blocking artifacts *** experiment results also verify that the reconstruction performance of BCS-ReSl0 is better than that of the BCSSPl in terms of noise tolerance at low measurement ratio.

关键词： compressed sensing (CS) block smoothed l0 norm (SlO)

Sublethal effects of sulfoxaflor on the fitness of two species of wheat aphids, Sitobion avenae(F.) and Rhopalosiphum padi(l.)

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Journal of Integrative Agriculture 2019年第7期18卷 1613-1623页

作者： XIN juan-juan YU Wen-xin YI Xiao-qing GAO Jun-ping GAO Xi-wu ZENG Xiao-peng Department of Entomology China Agricultural UniversityBeijing 100193P.R. China Haidian District Center for Disease Prevention and Control Beijing 100094P. R. China Beijing Center for Disease Prevention and Control Beijing 100013P.R. China

Sitobion avenae(F.) and Rhopalosiphum padi(l.) are two important pests of wheat in *** typically coexist in fields during the late period of wheat *** is a novel sulfoximine insecticide that demonstrates broad-spectrum efficacy,especially in targeting sap-feeding *** study was carried out to investigate the sublethal effects of sulfoxaflor on the development,longevity,and reproduction of two species of wheat *** results showed that sublethal concentrations of sulfoxaflor did not cause significant effects on the fecundity or the longevity of the parent generation(F0 generation) of either *** or ***,it caused transgenerational sublethal *** ***,adult longevity of F1 generation was significantly *** significant differences were observed on the population parameters of *** in the F1 *** ***,the adult preoviposition period(APOP) and the total preoviposition period(TPOP) of F1 generation were significantly *** mean generation time(T) was significantly reduced in the *** F1 ***’s more,the intrinsic rate of increase(rm) and the finite rate of increase(λ) were significantly increased in the *** F1 *** together,these results suggest that exposure to the lC25 of sulfoxafl had no effects on the parent generation of *** or ***,but it reduced adult longevity of *** as a negative effect and increased the rm and λ of *** in the first progeny generation,which may have an impact on the population dynamics of ***.

关键词： Sitobion avenae Rhopalosiphum padi sulfoxaflor sublethal effect

Activation of CFTR-mediated Cl^- Transport by Capsaicinoids in Cell Culture Model

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Chemical Research in Chinese Universities 2009年第2期25卷 220-223页

作者： ZHAO Xue-hang HOU Tmg-ting GE Hong SUN juan-juan YANG Hong MA Tong-hui Membrane Channel Research laboratory Northeast Normal University Changchun 130024 P. R. China liaoning Provincial Key laboratory of Biotechnology and Drug Discovery School of Life Sciences Liaoning Normal University Dalian 116029 P. R. China

Previous studies reported that capsaicin potentiates AF508 mutant cystic fibrosis transmembrane conductance regulator（CFTR） channel gating defect by transfected cell-based assays. It has been postulated that orally ingested capsaicin may conceptually be used to develop a therapeutic strategy to treat gastrointestinal disorders in CF patients. We tried to reproduce and extend those pre-clinical data of previous studies. Cell-based fluorescence func- tional measurements in Fischer thyroid epithelial cells（FRT） expressing CFTR showed no effect of capsaicin on potentiating AF508-CFTR, while genistein showed a strongly positive activity. Studies show that capsaicin and dihy- drocapsaicin activated cAMP-prestimulated wild-type CFTR in a dose-dependent manner with a maximal response of 70% of that activated by genistein, thus gave an apparent EC50 of （40.4±6.8）μmol/l and （150.2±7.4） μtmol/l respectively. Preliminary study shows that the binding sites for capsaicin and dihydrocapsaicin may be probably partially overlapped with that for genistein because the maximal activation of wild-type CFTR with genistein is partially blocked by caosaicin and dihydrocapsaicin.

关键词： Capsaicinoid Cystic fibrosis transmembrane conductance regulator(CFTR) Activation

Photosynthetic characteristics and nitrogen distribution of large-spike wheat in Northwest China

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Journal of Integrative Agriculture 2016年第3期15卷 545-552页

作者： WANG li-fang CHEN juan SHANGGUAN Zhou-ping State Key laboratory of Soil Erosion and Dryland Farming on the loess Plateau Northwest A&F University

The leaf photosynthesis and nitrogen（N） translocation in three large-spike lines and control cultivar（Xi＇nong 979） of winter wheat（Triticum aestivum l.） were studied in 2010–2011 and 2011–2012. The objectives of this study were to investigate the differences in the physiological characteristics of large-spike lines and control cultivar and identify the limiting factors that play a role in improving the yield of breeding materials. The average yield, grain number per spike, kernel weight per spike, and 1 000-kernel weight of the large-spike lines were 16.0, 26.8, 42.6, and 15.4%, respectively, significantly higher than those of control. The average photosynthetic rates（Pn） were not significant between the large-spike lines and control cultivar during the active growth period. The average PSII maximum energy conversion efficiency（Fv/Fm）, PSII actual quantum efficiency（Ф（PSII））, photochemical quenching coefficient（qP）, PSII reaction center activity（Fv′/Fm′） and water-use efficiency（WUE） of the large-spike lines were 1.0, 5.1, 3.6, 0.8, and 43.4%, respectively, higher than those of the control during the active growth stages. The N distribution proportions in different tissues were ranked in the order of grains〉culms＋sheathes〉rachis＋glumes〉flag leaves〉penultimate leaves〉remain leaves. This study suggested that utilization of the large-spike wheat might be a promising approach to obtain higher grain yield in Northwest China.

关键词： wheat nitrogen distribution large-spike lines photosynthetic characteristics yield

Effects of Silver Nanoparticles on Soil Microbial Communities and Bacterial Nitrification in Suburban Vegetable Soils

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Pedosphere 2017年第3期27卷 482-490页

作者： WANG juan SHU Kunhui ZHANG li SI Youbin School of Resources and Environment Anhui Agricultural University

Silver nanoparticles （AgNPs） are widely used antimicrobial compounds; however, they may pose a threat to non-targeted bacteria in the environment. In this study high-throughput sequencing was used to investigate the effects of different concentrations of AgNPs （10, 50, and 100 mg kg-1） on soil microbial community structure during short-term （7 d） exposure. The amounts of Acidobacteria, Actinobacteria, Cyanobacteria, and Nitrospirae significantly decreased with increasing AgNP concentration; meanwhile, several other phyla （e.g., Proteobacteria and Planctomycetes） increased and dominated. Nitrosomonas europaea, a well-characterized ammonia- oxidizing bacterium, was used to study the sensitivity of bacteria to AgNPs and ionic silver （Ag＋）. Flow cytometry was used to monitor the toxicity of low （1 mg l-l）, middle （10 mg l-l）, and high concentrations （20 mg l-1） of AgNPs, as well as Ag＋（1 mg l-1） released into the medium from 20 mg l-1 concentration of AgNPs, towards N. europaea. After 12 h of exposure, the survival rate of N. europaea treated with 1 mg l-1 Ag＋ was significantly lower than those treated with low （1 mg l-1） and middle concentrations （10 mg l-1） of AgNPs, but the survival rate in the treatment with high concentration （20 mg l-1） of AgNPs was much lower. Additionally, necrosis rates were higher in the treatment with 20 mg l-1 AgNPs. Electron microscopy showed that Ag＋ caused serious damage to the cell wall of N. europaea, disintegrated the nucleoids, and condensed next to the cell membrane; however, dissolved Ag＋ is only one of the antibacterial mechanisms of AgNPs.

关键词： ammonia-oxidizing bacterium cell necrosis cytotoxicity silver ions survival rate

Antenna Array Structures Effect on Water-Filling Capacity of Indoor NlOS MIMO Channel

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The Journal of China Universities of Posts and Telecommunications 2005年第3期12卷 17-20页

作者： lü Jian-gang lü Ying-hua DU juan lI Yun-zhuang WANG Xu-ying Continuing Education School Beijing University of Posts and Telecommunications Beijing 100876 P.R. China STMicroelectronics Beijing 100080 P.R. China China Telecommunication Corporation Beijing 100032 P.R. China

A 2-D Shooting and Bouncing Ray-tracing method （ SBR ） is used to analyze the different antenna array structure effect on the water-filling Capacity Complementary Cure ulative Distribution Functions （ CCDFS） of indoor Non-lineof-Sight （NlOS） Multiple-Input Multiple-Output （MIMO） channel. The results have shown that in NlOS indoor environment different antenna array structures affect on the CCDFS differently. The CCDFS of MIMO systems with antenna spacing 5λ change slightly with antenna array structures and all approach the in independent and identically distribution （ i. i.d. ） rayl,eigh channel water-filling capacity. When antenna spacing decreased to 0.53., the capacities of MIMO systerns drop also, and change with antenna array structures greatly. The results on outage water-filling capacity also show that there exist a fixed relationship that i. i.d. rayleigh channel capacity is larger than the capacity equipped with linear antenna array which is larger than the capacity equipped with rectangular antenna array and the capacity equipped with circular antenna array.

关键词： MIMO ray-tracing CCDFS antenna array structure water-filling principle

Zm HAK5 and Zm HAK1 function in K^+ uptake and distribution in maize under low K^+ conditions

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Journal of Integrative Plant Biology 2019年第6期61卷 691-705页

作者： Ya-juan Qin Wei-Hua Wu Yi Wang State Key laboratory of Plant Physiology and Biochemistry (SKlPPB) College of Biological SciencesChina Agricultural UniversityBeijing 100193China Center for Crop Functional Genomics and Molecular Breeding China Agricultural UniversityBeijing 100193China

Potassium (K^+) is an essential macronutrient for plant growth and development. Transporters from the KT/HAK/KUP family play crucial roles in K^+ homeostasis and cell growth in various plant species. However, their physiological roles in maize are still unknown. In this study, we cloned ZmHAKs and ZmHAKi and investigated their functions in maize (Zea mays l.). In situ hybridization showed that ZmHAKs was mainly expressed in roots, especially in the epidermis, cortex, and vascular bundle. ZmH AK5 was characterized as a high-affinity K^+ transporter. loss of function of ZmHAK5 led to defective K^+ uptake in maize, under low K^+ conditions, whereas ZmHAK5-over-expressing plants showed increased K^+ uptake activity and improved growth. ZmHAKi was upregulated under low K^+ stress, as revealed by RT-qPCR. ZmHAKi mediated K^+ uptake when heterologously expressed in yeast, but its transport activity was weaker than that of ZmHAK5. Overexpression of ZmHAKi in maize significantly affected K^+ distribution in shoots, leading to chlorosis in older leaves. These findings indicate that ZmHAKs and ZmHAKi play distinct roles in K^+ homeostasis in maize, functioning in K^+ uptake and K^+ distribution, respectively. Genetic manipulation of ZmHAK5 may represent a feasible way to improve K^+ utilization efficiency in maize.

关键词： ZmHAK5 and ZmHAK1 function in K^+uptake and distribution in maize under low K^+conditions^FA ZmHAK5 and ZmHAK1 function in K^+uptake distribution

nNOS and Neurological, Neuropsychiatric Disorders: A 20-Year Story

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Neuroscience Bulletin 2023年第9期39卷 1439-1453页

作者： li-juan Zhu Fei li Dong-Ya Zhu Key laboratory of Developmental Genes and Human Diseases Ministry of EducationDepartment of Histology and EmbryologySchool of MedicineSoutheast UniversityNanjing210009China Department of Pharmacology School of PharmacyNanjing Medical UniversityNanjing211166China Department of Medicinal Chemistry School of PharmacyNanjing Medical UniversityNanjing211166China

In the central nervous system,nitric oxide(NO),a free gas with multitudinous bioactivities,is mainly produced from the oxidation of l-arginine by neuronal nitric oxide synthase(nNOS).In the past 20 years,the studies in our group and other laboratories have suggested a significant involvement of nNOS in a variety of neurological and neuropsychiatric *** particular,the interactions between the PDZ domain of nNOS and its adaptor proteins,including post-synaptic density 95,the carboxy-terminal PDZ ligand of nNOS,and the serotonin transporter,significantly influence the subcellular localization and functions of nNOS in the *** nNOS-mediated protein-protein interactions provide new attractive targets and guide the discovery of therapeutic drugs for neurological and neuropsychiatric ***,we summarize the work on the roles of nNOS and its association with multiple adaptor proteins on neurological and neuropsychiatric disorders.

关键词： nNOS nNOS-PSD95 nNOS-CAPON nNOS-SERT Neurological and neuropsychiatric disorder

Endoscopic ultrasound evaluation in the surgical treatment of duodenal and peri-ampullary adenomas

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World Journal of Gastroenterology 2013年第4期19卷 511-515页

作者： lilian C Azih Brett l Broussard Milind A Phadnis Martin J Heslin Mohamad A Eloubeidi Shayam Varadarajulu juan Pablo Arnoletti Department of General Surgery University of Alabama BirminghamBirminghamAL 35233United States Department of Biostatistics Kansas University Medical CenterKansas CityKS 66160United States Department of Surgical Oncology University of Alabama BirminghamBirminghamAL 35233United States Department of Gastroenterology American University of BeirutBeirut 1107 2020Lebanon Department of Gastroenterology the Center for Specialized Surgery at Florida HospitalOrlandoFL 32804United States Department of Surgical Oncology The Center for Specialized Surgery at Florida HospitalOrlandoFL 32804United States

AIM:To investigate endoscopic ultrasound(EUS) for predicting depth of mucosal invasion and to analyze outcomes following endoscopic and transduodenal ***:Records of 111 patients seen at our institution from November 1999 to July 2011 with the postoperative pathological diagnosis of benign ampullary and duodenal adenomas were *** of patients who underwent preoperative EUS for diagnostic purposes were *** accuracy of EUS in predicting the absence of muscular invasion was assessed by comparing EUS reports to the final surgical pathological *** addition,the incidence of the post-operative complications over a period of 30 d and the subsequent long-term outcome(recurrence) over a period of 30 mo associated with endoscopic and transduodenal surgical resection was recorded,compared and ***:Among 111 patients with benign ampullary and duodenal adenomas,47 underwent preoperative EUS for 29 peri-ampullary lesions and 18 duodenal *** addition,computed tomography was performed in 18 patients,endoscopic retrograde cholangio-pancreatography in 10 patients and esophagogastroduodenoscopy in 22 *** were 43 patients with sporadic adenomas and 4 patients with familial adenomatous polyposis(FAP)/other polyposis *** 38(81%,P liably identified absence of submucosal and muscularis *** 4 cases,EUS underestimated submucosal invasion that was proven by *** the other 5 patients,EUS predicted muscularis invasion which could not be demonstrated in the resected *** predicted tumor muscularis invasion with a specificity of 88% and negative predictive value of 90%(P luded endoscopic resection in 22 cases,partial duodenectomy in 9 cases,transduodenal ampullectomy with sphincteroplasty in 10 cases and pancreaticoduodenectomy in 6 *** main post-operative final pathological results included villous adenoma(n = 5),adenoma(n =

关键词： Endoscopic ultrasound Duodenal periampullary adenoma Esophagogastroduodenoscopy Cholangio-pancreatography