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Pharmacology & Pharmacy 2023年第11期14卷 482-492页

作者： juan fabian Taylor Pierce Shenell Brown Jazmyne Smith Dolapo Adedeji Gloria Payne Department of Natural Sciences Elizabeth City State University Elizabeth City NC USA Department of Health & Human Studies Elizabeth City State University Elizabeth City NC USA

The combination of Artesunate (ART) and Paclitaxel (PTX) in two human prostate cancer (PCa) cell lines (PC-3 and LNCaP) was evaluated to investigate the effects on proliferation, apoptosis and morphological changes. The half maximal inhibitory concentration (IC50) values that were observed by ART and PTX on both LNCaP and PC-3 cell lines at 72-and 120-hour exposure were used to assess these effects. Early and late apoptosis was detected in Annexin V-FITC/PI assay revealed a shift in population of cells towards early and mid-apoptosis with ART + PTX than with ART and PTX individually. More effects were observed on LNCaP cell lines at both 72-hour and 120-hour exposure. The results for the Caspase 3/7 activity assay showed shift of viable population in all induced samples compared to control. Morphological changes occurred in both cell lines;this was validated in qualitative assessment when examined under the inverted microscope. These findings indicated that ART + PTX suppressed PCa cell proliferation in a dose- and time-dependent manner.

关键词： Combination Prostate Cancer Artesunate Paclitaxel Anticancer Activities

Regulation of the New Arabidopsis Imprinted Gene AtBMIIC Requires the Interplay of Different Epigenetic Mechanisms

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Molecular Plant 2012年第1期5卷 260-269页

作者： fabian Bratzel ChaoYang Alexandra Angelova Gema Lopez-Torrejon Marcus Koch juan Carlos del Pozo Myriam Calonje Centre for Organismal Studies Heidelberg Department of Biodiversity and Plant Systernatics University of Heidelberg Im Neuenheirner Feld 345 69120 Heidelberg Germany Centro de Biotecnologia y Genomica de Plantas （UPM-INIA） Campus de Montegancedo s/n Boadilla del Monte 28660 Madrid Spain

Recently, it has been shown that plants contain homologs to the animal Polycomb repressive complex I （PRC1） components BM11 and RINGIA/B. In Arabidopsis, there are three BMIl-like genes, two of which, AtBMIIA and B, are required during post-embryonic plant growth to repress embryonic traits and allow cell differentiation. However, little is known about the third BMIl-like gene, AtBMIIC. In this work, we show that AtBMIIC is only expressed during endosperm and stamen development. AtBMIIC is an imprinted gene expressed from the maternal allele in the endosperm but bialleli- cally expressed in stamen. We found that the characteristic expression pattern of AtBMIIC is the result of a complex epigenetic regulation that involves CG DNA methylation, RNA-directed non-CG DNA methylation （RdDM）, and PcG activity. Our results show the orchestrated interplay of different epigenetic mechanisms in regulating gene expression throughout development, shedding light on the current hypotheses for the origin and mechanism of imprinting in plant endosperm.

关键词： Epigenetics gene silencing development Polycomb Group proteins.

In vitro Evaluation with 5SBF of Alloy ASTM F75 Thermally Treated with Wollastonite

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Journal of Wuhan University of Technology(Materials Science) 2016年第3期31卷 487-490页

作者： MUNIZ Rodrigo ORTIZ juan LUNA Jesus MARTINEZ Jesus RODRIGEZ Nelly HERNANDEZ Marco OROZCO Pilar EQUIHUA fabian Faculty of Engineering Autonomous University of CoahuilaCUSaltilloMexico Department of Metal Mechanics Technological Institute of SaltilloMexico Faculty of Engineering Mechanical and Electrical Autonomous University of Nuevo LeonMexico Faculty of Engineering Autonomous University of Zacatecas Faculty of Mechanical and Electrical Engineering Autonomous University of Coahuila North UnitMonclovaMexico

Samples of a cobalt-based alloy that underwent a surface treatment were evaluated. The samples, which were obtained by casting alloy ASTM F 75, were ground and polished on one side until a mirror finish was obtained. The samples were encapsulated in wollastonite （W） using uniaxial pressure, treated at 1 220 ~C for 1 h and subsequently tempered in water. The characterisation of the sample indicated that part of the ceramic encapsulating material was mechanically incorporated on the metallic surface by growth of the oxide layer of the alloy. After thermal treatment, a series of specimens were submerged in a solution with 5-fold simulated body fluid （5SBF） for 3, 5 and 21 days. Characterisation by scanning electron microscopy （SEM）, energy dispersive spectroscopy （EDS） and X-ray diffraction （XRD） indicated nucleation and growth of a homogenous layer of apatite, beginning on the third day when the sample was submerged.

关键词： cobalt-based alloy wollastonite 5SBF apatite.

In the era of rapid mRNA-based vaccines:Why is there no effective hepatitis C virus vaccine yet?

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World Journal of Hepatology 2021年第10期13卷 1234-1268页

作者： Natalia Echeverria Victoria Comas fabian Aldunate Paula Perbolianachis Pilar Moreno juan Cristina Laboratorio de Virologia Molecular Centro de Investigaciones NuclearesFacultad de CienciasUniversidad de la RepublicaMontevideo 11400Uruguay Laboratorio de Evolucion Experimental de Virus Institut PasteurMontevideo 11400Uruguay Departamento de Desarrollo Biotecnologico Instituto de HigieneFacultad de MedicinaUniversidad de la RepublicaMontevideo 11600Uruguay

Hepatitis C virus(HCV)is responsible for no less than 71 million people chronically infected and is one of the most frequent indications for liver transplanta-tion *** direct-acting antiviral therapies fuel optimism in controlling HCV infections,there are several obstacles regarding treatment accessibility and reinfection continues to remain a ***,the majority of new HCV infections in developed countries occur in people who inject drugs and are more plausible to get *** achieve global epidemic control of this virus the development of an effective prophylactic or therapeutic vaccine becomes a *** coronavirus disease 19(COVID-19)pandemic led to auspicious vaccine development against severe acute respiratory syndrome coronavirus-2(SARSCoV-2)virus,which has renewed interest on fighting HCV epidemic with *** aim of this review is to highlight the current situation of HCV vaccine candidates designed to prevent and/or to reduce HCV infectious cases and their *** will emphasize on some of the crossroads encountered during vaccine development against this insidious virus,together with some key aspects of HCV immunology which have,so far,ham-pered the progress in this *** main focus will be on nucleic acid-based as well as recombinant viral vector-based vaccine candidates as the most novel vaccine approaches,some of which have been recently and successfully employed for SARS-CoV-2 ***,some ideas will be presented on which methods to explore for the design of live-attenuated vaccines against HCV.

关键词： Hepatitis C virus Vaccine candidates Nucleic acid-based vaccines Recombinant vector-based vaccines Challenges COVID-19

Eucalyptus grandis plantations:effects of management on soil carbon,nutrient contents and yields

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Journal of Forestry Research 2020年第2期31卷 601-611页

作者： Diego Martin Sandoval Lopez Marcelo fabian Arturi juan Francisco Goya Carolina Alejandra Perez Jorge Luis Frangi Laboratorio de Investigacion de Sistemas Ecologicos y Ambientales(LISEA) Universidad Nacional de La PlataDiagonal 113 N°4691900 La PlataBuenos AiresArgentina

The expansion of fast-growing tree plantations is a worldwide process,with consequences on soil fertility and soil carbon *** results were found on the effects of afforestation with Eucalyptus on soil carbon and other nutrient *** discrepancies are usually caused by differences in climate,land use history,soil texture as well as by management related factors such as plantation age,number of rotations,method of establishment(plantation or coppice),harvest residue management and soil *** studied the effect of plantation age,number of rotations,and method of establishment on soils and plant nutrient concentrations in Eucalyptus grandis plantations in NE Argentina on different textured *** also determined if yields changed with nutrient variations in soils,and compared soils under plantations to soils under grasslands they ***-one *** stands of different ages,number of rotations and method of establishment were evaluated as well as eight grassland *** of carbon,nitrogen,phosphorus,potassium,calcium and magnesium were determined for soils and *** carbon and nitrogen decreased over the number of rotations and were more pronounced in soils with 50-60%sand than soils with>75%*** stands showed higher soil carbon and nitrogen levels than plantations,suggesting a negative effect of site preparation before planting on soil nutrient conservation,especially in fine-textured *** nutrient concentrations did not follow the trends observed for soil nutrients nor did they reflect nutrient *** was no evidence of decreased yields over successive *** carbon and nitrogen contents decrease when grasslands are replaced by *** plantations,and therefore a yield limitation may occur in a medium to long-term frame,especially in stands re-established for short-rotation *** residue management and site preparation must be specifically designed for improving so

关键词： Soil texture Grasslands Foliar nutrient concentrations Successive harvest Coppice Eucalyptus grandis

Scarcity events analysis in adequacy studies using CN2 rule mining

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能源与人工智能(英文) 2022年第2期8卷 116-123页

作者： fabian Heymann Ricardo Bessa Mario Liebensteiner Konstantinos Parginos juan Carlos Martin Hinojar Pablo Duenas Center for Power and Energy Systems INESC TEC Porto Portugal School of Business Economics and Society Friedrich-Alexander-Universitat Erlangen-Nuremberg NurembergGermany System Development ENTSO-E Brussels Belgium MIT Energy Initiative Massachusetts Institute of Technology Cambridge(MA) United States PERSEE Mines ParisTech

Dealing with scarcity events is nowadays gaining relevance in electricity market studies, as traditionally predictable generation and consumption patterns are fading. Policymakers and system planners use therefore adequacy studies to a) understand if the current market design will attract sufficient generation capacity to meet electricity demand in the future and b) to comprehend what drives system inadequacy or resource scarcity when future scenarios lack adequate capacity. This work addressed the latter and showcases a first in-its-kind rulebased methodology that filters scarcity events from a large set of electricity market simulations. In this proof-of-concept, a rule-mining algorithm is applied to outputs from ENTSO-E’s Pan-European electricity market model, which is run for 700 model scenarios, each covering 8760 time steps. The developed methodology shows how to unveil potential reasons behind scarcity events in an automated, interpretable, and scalable manner.

关键词： Electricity markets Data mining Security of supply Renewable energy Interpretability

Prevalence of Primary Open Angle Glaucoma among Patients with Diagnosis of Systemic Hypertension and Diabetes Mellitus: The Colombian Glaucoma Study

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Open Journal of Ophthalmology 2020年第2期10卷 99-114页

作者： Carlos E. Rivera Erika Cantor Andres Castillo Alexander Martinez Lyle Newball juan C. Rueda Alejandro Valencia Sandra Belalcazar Tulio Cabal Oscar Albis-Donado fabian Mendez Fundación Oftalmológica del Valle Cali Colombia Institute of Statistics Universidad de Valparaiso Valparaiso Chile Department of Biology Faculty of Natural & Exact Sciences Universidad del Valle Cali Colombia Ophthalmology Residency Program Universidad del Valle Centro Médico Imbanaco Cali Colombia Clínica Lynd Newball San Andrés Islas Colombia FOSCAL Internacional Bucaramanga Colombia Glaucoma Unit Medellin Colombia Fundación Oftalmológica Nacional Bogotá Colombia Clínica Visión Cabal Buga Colombia Visual Sense Private Practice Mexico City Mexico School of Public Health GESP Group Universidad del Valle Cali Colombia

Objective: To establish the prevalence and risk factors of Primary Open Angle Glaucoma (POAG) among patients with Systemic Hypertension (SH) and Diabetes Mellitus (DM) in six cities of Colombia. Methods: A cross-sectional study among hypertensive and diabetic patients was conducted in Colombia. This study included 2067 subjects older than 50 years of age diagnosed with SH and/or DM. Participants underwent a complete ophthalmic examination including intraocular pressure (IOP) measurement by Goldmann tonometry and blood pressure measurement. The glaucoma diagnosis was confirmed by structural and functional evidence. Interviews and standardized questionnaires were used to evaluate participants’ lifestyle and other health conditions. Results: Among participants with DM/SH, 142 cases of POAG were confirmed for a prevalence of 5.6% [95% CI: 4.6 - 6.6], while 9.1% were glaucoma suspects [95% CI: 7.8% - 10.4%]. The majority of confirmed cases (77.5%) were undiagnosed. The prevalence of POAG was significantly higher with male gender, greater age, and diastolic blood pressure > 90 mmHg as risk factors. Conclusion: We found a high prevalence of POAG in patients with adequate SH and DM care in a novel Latino population. We also found great unawareness of the disease in this population. Our results have potentially enormous public health implications for Colombia and other Latino populations.

关键词： Open Angle-Glaucoma Glaucoma Systemic Hypertension Diabetes Mellitus

Superior biocompatibility and comparable osteoinductive properties: Sodium-reduced fluoride-containing bioactive glass belonging to the CaO–MgO–SiO2 system as a promising alternative to 45S5 bioactive glass

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Bioactive Materials 2020年第1期5卷 55-65页

作者： S.I.Schmitz B.Widholz C.Essers M.Becker D.U.Tulyaganov A.Moghaddam I.Gonzalo de juan F.Westhauser Center of Orthopedics Traumatology and Spinal Cord InjuryHeidelberg University HospitalSchlierbacher Landstraße 200a69118HeidelbergGermany Disperse Solid Materials Technical University DarmstadtOtto-Berndt-Straße 364287DarmstadtGermany Department of Natural-Mathematical Sciences Turin Polytechnic University in Tashkent17 Small Ring Street100095TashkentUzbekistan ATORG-Aschaffenburg Trauma and Orthopedic Research Group Center for Trauma SurgeryOrthopedicsand Sports MedicineKlinikum Aschaffenburg-AlzenauAm Hasenkopf 163739AschaffenburgGermany

Bioactive glasses(BGs)are promising bone substitute ***,under certain circumstances BGs such as the well-known 45S5 Bioglass®(composition in wt%:45.0 SiO2,24.5 Na2O,24.5 CaO,6.0 P2O5)act cytotoxic due to a strong increase in pH caused by a burst release of sodium ions.A potential alternative is a sodiumreduced fluoride-containing BG belonging to the CaO–MgO–SiO2 system,namely BG1d-BG(composition in wt%:46.1 SiO2,28.7 CaO,8.8 MgO,6.2 P2O5,5.7 CaF2,4.5 Na2O),that has already been evaluated in-vitro,in-vivo and in preliminary clinical *** further application,however,BG1d-BG should be compared to the benchmark amongst BGs,the 45S5 Bioglass®composition,to classify its effect on cell viability,proliferation and osteogenic differentiation of human mesenchymal stem cells(MSCs).Therefore,in this study,the biocompatibility and osteogenic potential of both BGs were investigated in an indirect and direct culture setting to assess the effect of the ionic dissolution products and the BGs’physical presence on the *** results indicated an advantage of BG1d-BG over 45S5 Bioglass®regarding cell viability and *** BGs induced an earlier onset of osteogenic differentiation and accelerated the expression of late osteoblast marker genes compared to the control *** conclusion,BG1d-BG is an attractive candidate for further experimental *** basic mechanisms behind the different impact on cell behavior should be assessed in further detail,*** further alteration of the BG compositions.

关键词： 45S5 Bioglass® Sodium-reduced bioactive glass Fluoride-containing bioactive glass Bone tissue engineering Human mesenchymal stromal cells

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集成电路电磁适应性的时钟信号调制(英文)

上海师范大学学报（自然科学版） 2010年第5期39卷 455-461页

作者：费利裴拉瓦迪蕾迪西亚波尔士法比安瓦嘎斯乔治赛米拉茱安安迪纳伊莎贝尔特谢拉保罗特谢拉格兰德天主教大学PUCRS 埃尔加夫大学INESC-ID 维果大学 IST INESC-ID

电磁干扰(EMI)是瞬时功能故障的主要来源之一,原因是电源供电线上的噪声注入引起了VDD和GND的额定值的波动.介绍了一种新的方法来增强片上系统(SoC)关于电源和接地电压瞬变时的信号完整性,并且这种方法完全符合IEC 61000—4—29标准.其... 详细信息

电磁干扰(EMI)是瞬时功能故障的主要来源之一,原因是电源供电线上的噪声注入引起了VDD和GND的额定值的波动.介绍了一种新的方法来增强片上系统(SoC)关于电源和接地电压瞬变时的信号完整性,并且这种方法完全符合IEC 61000—4—29标准.其基本思想是设计和IEC 61000—4—29兼容的集成电路,通过局部和动态地将时钟占空比去适应传播延迟的变化和扰动的逻辑路径.当无法满足时,该方法导致暂时把集成电路的工作频率减小到满足该标准的最小值.根据异常电网活动,时钟占空比调制(CDCM)是通过使用正和/或负边沿时钟展宽逻辑(CSL)块来实现的.基于这个概念,在尽可能保持时钟高速频率的同时,数字电路对于电源供电线上波动的耐受性将会更强.该方法可被视作一种在线提供动态自适应同步的监视技术.通过SPICE模拟,实验结果表明此方法的有效性.

关键词：信号完整性 IEC 61000—4—29 集成电路