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Isolation and Characterization of Mercury Resistant Trichoderma Strains from Soil with High Levels of Mercury and Its Effects on Arabidopsis thaliana Mercury Uptake

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Advances in Microbiology 2018年第7期8卷 600-613页

作者： JoséLuis Hernández-Flores JoséGerardo Bernal Melo Andrés Cruz Hernández Miguel A.Ramos López Carlos Saldana Gutiérrez Sergio Romero Gomez Victor Pérez Moreno Rosa P.Calvillo Medina JoséHumberto Valenzuela Soto Sergio Pacheco Hernández George H.Jones Sergio Casas Flores juan campos-guillen Laboratorio de Bioseguridad y Análisis de Riesgo Departamento de Ingeniería Genética de PlantasCentro de Investigación y de Estudios Avanzados del IPNIrapuatoGto.México Facultad de Química Universidad Autónoma de QuerétaroCerro de las Campanas s/nQuerétaroQro.México Escuela de Agronomía Universidad De La Salle Bajíocampus Campestre.LeónGto.México Facultad de Ciencias Naturales Universidad Autónoma de QuerétaroAv.De las Ciencias S/NQuerétaroQro.México Centro de Investigación en Química Aplicada Blvd.Enrique Reyna Hermosillo No.140SaltilloCoahuilaMéxico Department of Biology Emory UniversityAtlantaGeorgiaUSA IPICYT División de Biología Molecular.Camino a la presa San JoséNo.2055Colonia Lomas 4a sección.C.P.San Luis PotosíMexico

Traditional mining activities are usually correlated with high levels of soil pollution, which is a major environmental concern. Extensive mining activities have taken place in the San Joaquin region in the State of Querétaro, México resulting in high levels of mercury soil pollution (up to 1532 ± 300 mg/kg). We isolated mercury-resistant fungal strains from the San Joaquin region soils and identified them through morphologic characteristics and ITS rDNA region sequence analysis. We determined that fungi isolated belong to the genus Trichoderma. All the isolates selected showed the ability to catalyze the volatilization of Hg. For air sampling, an active sampling device was constructed and using acid KMnO4 as an absorbent, the concentration of mercury in solution was determined through the cold vapor atomic absorption method. The results show mercury volatilization from the fungal species assay, with a maximum of 213.04 ± 32.6 μg/m3 while mycelium accumulation ranged from less than 17.5 ± 2.9 to 20.0 ± 3.4 μg/g. The fungal isolates were also evaluated for their ability to reduce mercury uptake in Arabidopsis thaliana. These observations suggest the utility of Trichoderma for the mobilization of mercury in those contaminated soils.

关键词： Volatilization Mercury Trichoderma Soil Bioremediation

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A multidimensional review of the cash management problem

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Financial Innovation 2023年第1期9卷 1840-1874页

作者： Francisco Salas‑Molina juan A.Rodríguez‑Aguilar Montserrat guillen Universitat Politècnica de València Ferrándiz y Carbonell03801 AlcoySpain IIIA‑CSIC Campus UAB08913 CerdanyolaSpain Universitat de Barcelona Av.Diagonal69008034 BarcelonaSpain

In this paper,we summarize and analyze the relevant research on the cash manage-ment problem appearing in the ***,we identify the main dimensions of the cash management ***,we review the most relevant contributions in this field and present a multidimensional analysis of these contributions,according to the dimensions of the *** this analysis,several open research questions are highlighted.

关键词： Cash management Models Dimensions Review

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Interaction of piRNA-like sequences from the 3′-UTR of SARS-CoV-2 with mRNA regions

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Genes & Diseases 2023年第6期10卷 2282-2284页

作者： Laura Pérez-campos Mayoral María Teresa Hernández-Huerta Carlos Romero Díaz Carlos Alberto Matias-Cervantes Eduardo Pérez-campos Mayoral Margarito Martínez Cruz juan de Dios Ruiz-Rosado Edgar Gustavo Ramos Martínez Marco Antonio Sánchez Medina Eduardo Pérez-campos Research Center Faculty of Medicine UNAM-UABJOAutonomous University“Benito Juárez”of Oaxaca(UABJO)Oaxaca 68020Mexico CONACyT Faculty of Medicine and Surgery.Autonomous University“Benito Juárez”of Oaxaca(UABJO)Oaxaca 68020Mexico National Technology of Mexico(TecNM)/IT Oaxaca Oaxaca de JuárezOaxaca 68030Mexico The Research Institute at Nationwide Children's Hospital Columbus 43205USA Facultad de Química Universidad Nacional Autónoma de MéxicoCoyoacán 04510Mexico

Piwi-interacting RNAs(piRNAs of 26-32 nt in length)regulate gene expression,epigenetics,and transcriptional and post-transcriptional processes.1 In humans,piRNAs are expressed in germline and somatic tissues,and generally have a uracil at the 5'-end(position+1),an adenine at the+10 position,and are 2'-0 methylated at the 3'-end.2 Onice piRNAs form the RNA-induced silencing complex(RISC)with PIWl proteins,they serve as guides to find their complementary sequences of the target mRNA,thus initiating their *** Wuhan patient's genome is approximately 265 nucleotides(nts)in its 3'-UTR;piRNAs-like sequences have been reported in this region.3 Our objective was to search,through bioinformatics,for mRNA sequences that were homologous to the reverse complementary of the previously reported piRNA-like sequences from the 3'-UTR of SARS-Cov-2,3 and identify the possible interaction between them.

关键词： sequences complementary initiating

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Depolarizing metrics in the biomedical field:Vision enhancement and classification of biological tissues

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Journal of Innovative Optical Health Sciences 2023年第5期16卷 3-19页

作者： Mónica Canabal-Carbia Albert Van Eeckhout Carla Rodríguez Emilio González-Arnay Irene Estévez JoséJ.Gil Enrique García-Caurel Razvigor Ossikovski juan campos Angel Lizana Grup d'Òptica Departamento de Fisica Universitat Autònoma de BarcelonaBellaterra 08193Spain ALBA Synchrotron Light Source Carrer de la Llum 2-26 08290 Cerdanyola del VallèsBarcelonaSpain Servicio deAnatomia Humana Departamento de Ciencias Médicas Básicas Universidad de La Laguna Santa Cruz de Tenerife 38200 Spain Universidad de Zaragoza Pedro Cerbuna 12 Zaragoza 50009Spain LPICM CNRSEcole Polytechnique Institut Politechnique de ParisPalaiseau 91120France

Polarimetry encompasses a collection of optical techniques broadly used in a variety of ***,such techniques have provided their suitability in the biomedical field through the study of the polarimetric response of biological samples(retardance,dichroism and depolarization)by measuring certain polarimetric *** of these features,depolarization,is mainly produced by scattering on samples,which is a predominant efiect in turbid media as biological *** turn,retardance and dichroic efiects are produced by tissue anisotropies and can lead to depolarization *** depolarization is a predominant efiect in tissue samples,we focus on studying difierent depolarization metrics for biomedical *** report the suitability of a set of depolarizing observables,the indices of polarimetric purity(IPPs),for biological tissue *** review some results where we demonstrate that IPPs lead to better performance than the depolarization index,which is a well-established and commonly used depolarization observable in the *** also provide how IPPs are able to significantly enhance contrast between difierent tissue structures and even to reveal structures hidden by using standard intensity ***,we also explore the classificatory potential of IPPs and other depolarizing observables for the discrimination of difierent tissues obtained from ex vivo chicken samples(muscle,tendon,myotendinous junction and bone),reaching accurate models for tissue classification.

关键词： Polarimetry indices of polarimetric purity organic tissues visualization artificial intelligence.

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Endothelial progenitor cells as a therapeutic option in intracerebral hemorrhage

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Neural Regeneration Research 2017年第4期12卷 558-561页

作者： juan Pías-Peleteiro Francisco campos José Castillo Tomás Sobrino Clinical Neurosciences Research Laboratory Department of NeurologyStroke UnitUniversity Clinical HospitalUniversidade de Santiago de CompostelaHealth Research Institute of Santiago de Compostela (IDIS)

Intracerebral hemorrhage （ICH） is the most severe cerebrovascular disease, which represents a leading cause of death and disability in developed countries. However, therapeutic options are limited, so is mandatory to investigate repairing processes after stroke in order to develop new therapeutic strategies able to promote brain repair processes. Therapeutic angiogenesis and vasculogenesis hold promise to improve outcome of ICH patients. In this regard, circulating endothelial progenitor cells （EPCs） have recently been suggested to be a marker of vascular risk and endothelial function. Moreover, EPC levels have been associated with good neurological and functional outcome as well as reduced residual hematoma volume in ICH patients. Finally, experimental and clinical studies indicate that EPC might mediate endothelial cell regeneration and neovascularization. Therefore, EPC-based therapy could be an excellent therapeutic option in ICH. In this mini-review, we discuss the present status of knowledge about the possible therapeutic role of EPCs in ICH, molecular mechanisms, and the future perspectives and strategies for their use in clinical practice.

关键词： cellular therapy endothelial progenitor cells growth factors intracerebral hemorrhage neurorepair outcome

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Diversity of plants and mammals as indicators of the effects of land management types in woodlands

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Forest Ecosystems 2021年第4期8卷 991-1005页

作者： Carolina R.Szymanski Solana Tabeni juan A.Alvarez Claudia M.campos Instituto Argentino de Nivologia Glaciologia y Ciencias AmbientalesCCT CONICET MendozaAv.Ruiz Leal s/nParque General San Martin5000 MendozaArgentina Facultad de Ciencias Agrarias Universidad Nacional de CuyoAlte.Brown s/nChacras de Coria5505 MendozaArgentina Instituto Argentino de Investigaciones de las Zona Aridas(UNCuyo-Gobierno de Mendoza-CONICET) CCT CONICET MendozaAv.Ruiz Leal s/nParque General San Martin5000 MendozaArgentina

Background:The ecological indicators are useful tools to determine the effects of human disturbances on woodland ***,ecological indicators not always responded in the same way to disturbances,and the responses can differ among *** arid and semiarid woodlands,the use of deadwood associated with cattle raising can affect biodiversity and Nature’s contributions to ***:Our study aimed to assess changes in taxonomic and functional diversity of two assemblages,plants and mammals,in Prosopis woodlands under different land management types:grazed woodlands and a protected *** plants,changes in structural diversity were also *** trees under different land management types were selected and their deadwood characteristics were *** live traps and camera traps,we obtained data on the presence-absence of mammals per tree to estimate diversity *** plants,we measured the abundance of vegetation by species and by cover type through the Line-Intercept Method to estimated ***,we built generalized linear models to assess the responses of diversity of each assemblage to covariables concerning deadwood and different land management ***:We found that all diversity indeces for plants were either negatively affected by the presence of deadwood on the ground,or favored by its *** mammals,removal of deadwood increased taxonomic diversity,while functional diversity increased with deadwood on the *** structural diversity of plants and functional diversity of mammals were greater in grazed ***:The sustainable use of woodland resources is essential for the activities of rural *** study results indicated that land management of grazed woodlands promoted the structural diversity of plant assemblages and the functional diversity of *** presence of deadwood negatively affected plant diversity but it increased mammal functional *** is a

关键词： Central Monte Cattle raising Deadwood extraction Taxonomic diversity Functional traits

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Irbesartan desmotropes:Solid-state characterization,thermodynamic study and dissolution properties

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Journal of Pharmaceutical Analysis 2019年第5期9卷 339-346页

作者： Andrea Mariela Araya-Sibaja Carlos Eduardo Maduro de campos Cinira Fandaruff Jose Roberto Vega-Baudrit Teodolito guillen-Giron Mirtha Navarro-Hoyos Silvia Lucía Cuffini Laboratorio Nacional de Nanotecnología LANOTEC-CeNAT-CONARE 1174-1200 Pavas San Jose Costa Rica Escuela de Ciencia e Ingeniería de los Materiales Tecnologico de Costa Rica Cartago 159-7050 Costa Rica Escuela de Química Universidad de Costa Rica San Pedro de Montes de Oca 2060 San Jose Costa Rica Programa de Pos-Graduacao em Física Universidade Federal de Santa Catarina Florianopolis Brazil Independent Researcher 06472-005 Barueri Sao Paulo Brazil Laboratorio de Investigacion y Tecnología de Polímeros POLIUNA Escuela de Química Universidad Nacional de Costa Rica Heredia 86-3000 Costa Rica Instituto de Ciencia e Tecnica Universidade Federal de Sao Paulo Sao Jose dos Campos Sao Paulo Brazil

Irbesartan (IBS) is a tetrazole derivative and antihypertensive drug that has two interconvertible structures, 1H- and 2H-tautomers. The difference between them lies in the protonation of the tetrazole ring. In the solid-state, both tautomers can be isolated as crystal forms A (1H-tautomer) and B (2H-tautomer). Studies have reported that IBS is a polymorphic system and its forms A and B are related monotropically. These reports indicate form B as the most stable and less soluble form. Therefore, the goal of this contribution is to demonstrate through a complete solid-state characterization, thermodynamic study and dissolution properties that the IBS forms are desmotropes that are not related monotropically. However, the intention is also to call attention to the importance of conducting strict chemical and in solid-state quality controls on the IBS raw materials. Hence, powder X-ray diffraction (PXRD) and Raman spectroscopy (RS) at ambient and non-ambient conditions, differential scanning calorimetry (DSC), hot stage microscopy (HSM), Fourier transform infrared (FT-IR) and scanning electron microscopy (SEM) techniques were applied. Furthermore, intrinsic dissolution rate (IDR) and structural stability studies at 98% relative humidity (RH), 25℃ and 40 ℃ were conducted as well. The results show that in fact, form A is approximately four-fold more soluble than form B. In addition, both IBS forms are stable at ambient conditions. Nevertheless, structural and/or chemical instability was observed in form B at 40℃ and 98% RH. IBS has been confirmed as a desmotropic system rather than a polymorphic one. Consequently, forms A and B are not related monotropically.

关键词： Irbesartan Solid-state characterization Intrinsic dissolution rate Tautomerism Desmotropic forms

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αKLOTHO and sTGFβR2 treatment counteract the osteoarthritic phenotype developed in a rat model

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Protein & Cell 2020年第3期11卷 219-226页

作者： Paloma Martinez-Redondo Isabel guillen-guillen Noah Davidsohn Chao Wang Javier Prieto Masakazu Kurita Fumiyuki Hatanaka Cuiqing Zhong Reyna Hernandez-Benitez Tomoaki Hishida Takashi Lezaki Akihisa Sakamoto Amy NNemeth Yuriko Hishida Concepcion Rodriguez Esteban Kensaku Shojima Ling Huang Maxim Shokhirev Estrella Nunez-Delicado Josep MCampistol Isabel guillen-Vicente Elena Rodriguez-Inigo juan Manuel Lopez-Alcorocho Marta guillen-Vicente George Church Pradeep Reddy Pedro guillen-Garcia Guang-Hui Liu juan Carlos Izpisua Belmonte Gene Expression Laboratory Salk Institute for Biological Studies10010 North Torrey Pines RoadLa JollaCA 92037USA Wyss Institute for Biologically Inspired Engineering Harvard UniversityCambridgeMA 02115USA Department of Genetics Harvard Medical SchoolBostonMA 02115USA Integrative Genomics and Bioinformatics Core Salk Institute for Biological Studies10010 North Torrey Pines RoadLa JollaCA 92037USA Universidad Catolica San Antonio de Murcia(UCAM) Campus de los JeronimosNo 1351230107 GuadalupeSpain Hospital Clinic of Barcelona Career Villarroel17008036 BarcelonaSpain Department of Traumatology and Research Unit Clinica CEMTRO28035 MadridSpain National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Institute for Stem cell and Regeneration Chinese Academy of SciencesBeijing 100101China Translational Medical Center for Stem Cell Therapy Shanghai East HospitalTongji University School of MedicineShanghai 200120China

Dear Editor,Homeostasis and repair are critical biological processes that allow for tissue and organ preservation and function in multicellular *** regulation and extension vary drastically across the animal kingdom,and mammals show limited tissue-specific regenerative capacity that declines with *** aging,articular cartilage is one of the tissues that undergo substantial changes in the matrix structure,molecular composition,metabolic activity,and mechanical properties(Loeser et al.2016).

关键词： aging treatment critical

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Foliar persistence and residual activity of methoxyfenozide against beet armyworm （Lepidoptera： Noctuidae）

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Insect Science 2013年第6期20卷 734-742页

作者： Omar-Ulises Aguirre Ana-Mabel Martinez Jesus campos-Garcia Luis-Antonio Hernaindez Jose-Isaac Figueroa Philippe Lobit Elisa Vinuela juan-Manuel Chavarrieta Guy Smagghe Samuel Pineda Instituto de Investigaciones Agropecuarias y Forestales Universidad Michoacana de San Nicolas de Hidalgo Michoacan Instituto de Investigaciones Quimico Biologicas Universidad Michoacana de San Nicolas de Hidalgo Edif. B-3 Ciudad Universitaria CP 58030 Morelia Michoacan Mexico Proteccion de Cultivos Escuela Tecnica Superior de Ingenieros Agronomos Madrid E-28040 Spain Department of Crop Protection Faculty of Bioscience Engineering Ghent University Ghent B-9000 Belgium

A greenhouse study was conducted to investigate the persistence of methoxyfenozide in pepper （Capsicum annuum L.） foliage. An aqueous suspension of methoxyfenozide was sprayed on pepper plants at concentrations of 72 and 144 mg of active ingredient （a.i.）/L. Foliage was collected at different intervals of time （0, 3, 5, 10, 20, 30, 40, and 50 days） after the treatment, and the methoxyfenozide residue was measured by high-performance liquid chromatography. The foliage was also used in bioas- says to determine the residual toxicity on and the consumption rate of the third-instar larvae of the beet armyworm, Spodoptera exigua （Htibner）（Lepidoptera： Noctuidae）. The methoxyfenozide concentrations observed after 50 days had decreased to 19 and 69μg/g per sample, corresponding to a loss of 61% and 28% from the application concentrations of 72 and 144 mg a.i./L, respectively. When fitting a first-order kinetics degradation model, the half-life （DT50） of this compound was 76 days. Both application concentrations of methoxyfenozide caused a high mortality rate （〉97%） when the larvae were fed the pepper foliage collected at all of the time intervals. Lastly, at all of the time points, the consumption rate by the larvae was reduced to between 57% and 92% for both concentrations that were bioassayed. Our results indicate that, under the present greenhouse conditions, the degradation of methoxyfenozide was slower than that reported by other authors and that its residues were highly toxic to S. exigua larvae. The implications of these results for the management programs of this pest are discussed.

关键词： consumption rate foliar persistence methoxyfenozide residue residualactivity Spodoptera exigua

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Hepatocellular carcinoma and hepatitis C virus infection in Latin America: epidemiology, diagnosis and treatment

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Hepatoma Research 2020年第5期6卷 1-18页

作者： Marina Galicia-Moreno Hugo Christian Monroy-Ramirez Marina campos-Valdez Jaime Sanchez-Meza Laura Sanchez-Orozco juan Armendariz-Borunda University of Guadalajara Institute of Molecular Biology in Medicine and Gene TherapyDepartment of Molecular Biology and GenomicsHealth Science University Center(CUCS)Jalisco 44340Mexico Tecnologico de Monterrey Campus GuadalajaraJalisco 45138Mexico

Hepatocellular carcinoma(HCC)is the most common cancer associated with chronic liver disease and *** most common cause of HCC is chronic hepatitis C virus infection and many studies in Europe,Asia and North America have focused on its etiology,epidemiology,diagnostic tools,and therapeutic ***,little is known about these issues in Latin *** aim of this review is to address these aspects of HCC in Latin *** main risk factors associated with developing HCC in this region are:age,concomitant cirrhosis,hepatitis C infection,obesity and hereditary disease such as *** the other hand,screening tests and diagnostic methods of HCC are mostly serum alpha fetoprotein quantification,liver ultrasound,computed tomography,magnetic resonance,and *** diagnostic methods include gut microbiota analysis and the use of nanotechnology and they continue to be ***,according to the Barcelona Clinic Liver Cancer,curative treatments used in HCC patients are mainly liver resection,liver transplantation,and local ablation,each with advantages and *** conclusion,clear strategies are urgently needed to understand the extent of HCC and related problems in this part of the *** review provides greater knowledge of HCC for the proper design of preventive programs by taking into consideration specific characteristics of our ***,this review allows for an understanding of individualizing treatments according to the patient’s needs.

关键词： Liver hepatitis C epidemiology diagnosis treatment hepatocellular carcinoma Latin America

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