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Safety of intravitreal quinupristin/dalfopristin in an animal model

International Journal of Ophthalmology(English edition) 2016年第3期9卷 373-378页

作者： Veronica E.Giordano Sergio E.Hernandez-Da Mota Tania N.A dabache-Guel Armando Castillejos-Cheve Sonia Corredor-Casas Samantha M.Salinas-Longoria Rafael Romero-Vera juan M.Jimenez-Sierra Jose L.Guerrero-naranjo Virgilio Morales-Canton Department of Ophthalmology Retina and Vitreous Service Asociacion para Evitar la Ceguera en Mexico Vicente Garcia Torres 46 Coyoacan Barrio San Lucas Mexico City 04030 Mexico Ophthalmology Service Clinica David Boulevard Garcia de Leon 598 Nueva Chapultepec Morelia Michoacan 58280Mexico Department of Ophthalmology Glaucoma Service Asociacion para Evitar la Ceguera en Mexico Vicente Garcia Torres 46 Coyoacan Barrio San Lucas Mexico City 04030 Mexico Department of Ophthalmology Pathology Service Asociacion para Evitar la Ceguera en Mexico Vicente Garcia Tortes 46 Coyoacan Barrio San Lucas Mexico City 04030 Mexico

AIM： To determine whether different intravitreal doses of quinupristin/dalfopristin lead to electroretinographic or histological changes in the rabbit retina over one month period after injection. METHODS： Eighteen New Zealand white rabbits were divided into three treatment groups （groups 1 to 3） and different intravitreal doses of quinupristin/dalfopristin were tested in each group. The right eye was injected with the drug and the left eye received intravitreal injection of 5% dextrose water and served as control eye. The doses delivered to each group were 0.1 mg/0.1 mL, 1 mg/0.1 mL and 10 mg/0.1 mL. Simultaneous, bilateral, dark-adapted electroretinography and clinical images of both eyes were obtained in all groups before injection （baseline） and after 7, 14, 21 and 28d, followed by enucleation for histological examination. RESULTS： Subjects in the group 1 showed no signs of toxicity in the electroretinogram when compared with groups 2 and 3 （Kruskall-Wallis test, P=0.000）. By day 7, no electrical response to light stimuli was recorded in the treated eyes in groups 2 and 3, consistent with severe damage due to retinal toxicity. Light microscopy revealedno significant histopathological changes in the group 1, while rabbits in groups 2 and 3 had signs of granulomatous inflammation in most cases. CONCLUSION： Intravitreal 0.1 mg/0.1 mL doses of quinupristin/dalfopristin do not lead to electroretinographic or histological signs of retinal toxicity compared with 1 mg/0.1 mL and 10 mg/O.1 mL in this rabbit model.

关键词： endophthalmitis quinupristin/dalfopristin retinal toxicity

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Statistical data driven approach of COVID-19 in Ecuador: R_(0) and R_(t) estimation via new method

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Infectious Disease Modelling 2021年第1期6卷 232-243页

作者： Raúl Patricio Fernandez-naranjo MSc Eduardo Vasconez-Gonzalez MD Katherine Simbana-RiveraMD,MSc Lenin Gomez-Barreno MD juan S.Izquierdo-Condoy MD Domenica Cevallos-RobalinoMD,MPH(c) Esteban Ortiz-Prado MD,MSc,MPH One Health Research Group Faculty of MedicineUniversidad de las AmericasQuitoEcuador Resident of Intensive Care Unit Hospital Quito Sur IESSQuitoEcuador

The growth of COVID-19 pandemic throughout more than 213 countries around the world have put a lot of pressures on governments and health services to try to stop the rapid expansion of the *** 2009,H1N1 Influenza pandemic,statistical and mathematical methods were used to track how the virus spreads around *** of these models that were developed at the beginning of the XXI century are based on the classical susceptible-infected-recovered(SIR)model developed almost a hundred years *** evolution of this model allows us to forecast and compute basic and effective reproduction numbers(R_(t) and R_(0)),measures that quantify the epidemic potential of a pathogen and estimates different *** this study,we present a traditional estimation technique for R_(0) with statistical distributions by best fitting and a Bayesian approach based on continuous feed of prior distributions to obtain posterior distributions and computing real time R_(t).We use data from COVID-19 officially reported cases in Ecuador since the first confirmed case on February *** of the lack of data,in the case of R_(0) we compare two methods for the estimation of these parameters below exponential growth and maximum likelihood *** do not make any assumption about the evolution of cases due to limited information and we use previous methods to compare scenarios about R_(0) and in the case of R_(t) we used Bayesian inference to model uncertainty in contagious proposing a new modification to the well-known model of Bettencourt and Ribeiro based on a time window of m days to improve *** R_(0) with exponential growth criteria was 3.45 and with the maximum likelihood estimation method was *** results show that Guayas,Pichincha and Manabíwere the provinces with the highest number of cases due to *** reasons explain the increased transmissibility in these localities:massive events,population density,cities dispersion patterns,and

关键词： Bayesian inference R0 COVID-19 Real time reproduction factor Basic reproduction factor Ecuador

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Pathogenesis of glomerular haematuria

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World Journal of Nephrology 2015年第2期4卷 185-195页

作者： Claudia Yuste Eduardo Gutierrez Angel Manuel Sevillano alfonso Rubio-Navarro juan Manuel Amaro-Villalobos Alberto Ortiz Jesus Egido Manuel Praga juan Antonio Moreno Department of Nephrology Gregorio Mara?on Hospital Department of Nephrology Renal Vascular and Diabetes Research LabⅡS-Fundación Jiménez Díaz Autonoma University (UAM) Renal Vascular and Diabetes Research LabⅡS-Fundación Jiménez DíazAutonoma University (UAM)

Haematuria was known as a benign hallmark of some glomerular diseases, but over the last decade, new evidences pointed its negative implications on kidneydisease progression. Cytotoxic effects of oxidative stress induced by hemoglobin, heme, or iron released from red blood cells may account for the tubular injury observed in human biopsy specimens. However, the precise mechanisms responsible for haematuria remain unclear. The presence of red blood cells （RBCs） with irregular contours and shape in the urine indicates RBCs egression from the glomerular capillary into the urinary space. Therefore glomerular haematuria may be a marker of glomerular filtration barrier dysfunction or damage. In this review we describe some key issues regarding epidemiology and pathogenesis of haematuric diseases as well as their renal morphological fndings.

关键词： Haematuria Pathogenesis Glomerular filtration barrier Dysmorphic red blood cells Chronic kidney disease Microscopic haematuria

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Ecological-Genetic Studies and Conservation of Endemic <i>Quercus sideroxyla</i>(Trel.) in Central Mexico

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Natural Resources 2014年第9期5卷 442-453页

作者： Cecilia alfonso-Corrado Ricardo Clark-Tapia Alejandro Monsalvo-Reyes Carlos Rosas-Osorio Gabriel González-Adame Francisco naranjo-Luna Crystian-Sadiel Venegas-Barrera Jorge E. Campos Instituto de Estudios Ambientales Universidad de la Sierra Juárez Ixtlán de Juárez México InstitutoTecnológico de Ciudad Victoria Ciudad Victoria México Laboratorio de Bioquímica Molecular-Unidad de Biotecnología y Prototipos (UBIPRO) Fes-IZTACALA UNAM Tlalnepantla México

This study examines the distribution, ecology and genetic diversity of Quercus sideroxyla Humb. Bonpl, with an emphasis on its conservation on the Natural Protected Area of Sierra Fría in North Central Mexico. Twenty-nine locations were selected, and in each location, one plot of 1500 m2 was established. At every location, we obtained an abundance of Q. sideroxyla and a basal area for each individual tree, including geographical and climatological data. We used the Outlying Mean Index (OMI) to examine whether environmental conditions had a distributional effect on Q. sideroxyla populations and to obtain the static size population structure of the species. For the genetic analysis, we collected 18 adult individuals from each population, four polymorphic loci were used to estimate genetic diversity. Q. sideroxyla abundance was associated with narrow environmental conditions, especially when considering the topographical and meteorological environmental variables. The allelic richness value was 84 alleles (21 privatealleles), and the expected mean heterozygosity was 0.855 ± 0.009. The high vulnerability of the species to changes in the land use at the local scale and to global climatic changes increases the species’ susceptibility to local disappearance.

关键词： Ecology Genetic Diversity Local Conservation Niche Oaks

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Novel TINF2 gene mutation in dyskeratosis congenita with extremely short telomeres:A case report

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World Journal of Clinical Cases 2022年第33期10卷 12440-12446页

作者： Verónica Judith Picos-Cárdenas Saúl Armando Beltrán-Ontiveros Joséalfonso Cruz-Ramos JoséAlfredo Contreras-Gutiérrez Eliakym Arámbula-Meraz Carla Angulo-Rojo Alma Marlene Guadrón-Llanos Emir Adolfo Leal-León Dora María Cedano-Prieto juan Pablo Meza-Espinoza Laboratorio de Genética Facultad de MedicinaUniversidad Autónoma de SinaloaCuliacán 80018SinaloaMexico Centro de Investigación y Docencia en Ciencias de la Salud Hospital Civil de CuliacánUniversidad Autónoma de SinaloaCuliacán 80030SinaloaMexico Departamento de Clínicas Médicas Centro Universitario de Ciencias de la SaludUniversidad de GuadalajaraGuadalajara 44340JaliscoMexico Facultad de Medicina Universidad Autónoma de SinaloaCuliacán 80018SinaloaMexico Laboratorio de Genética y Biología Molecular Facultad de Ciencias Químico-BiológicasUniversidad Autónoma de SinaloaCuliacán 80010SinaloaMexico Laboratorio de Neurociencias Facultad de MedicinaUniversidad Autónoma de SinaloaCuliacán 80018SinaloaMexico Laboratorio de Diabetes y Comorbilidades Facultad de MedicinaUniversidad Autónoma de SinaloaCuliacán 80018SinaloaMexico Facultad de Medicina Universidad Autónoma de TamaulipasMatamoros 87349TamaulipasMexico

BACKGROUND Dyskeratosis congenita is a rare disease characterized by bone marrow failure and a clinical triad of oral leukoplakia,nail dystrophy,and abnormal skin *** genetics of dyskeratosis congenita include mutations in genes involved in telomere maintenance,including *** SUMMARY Here,we report a female patient who presented thrombocytopenia,anemia,reticulate hyperpigmentation,dystrophy in fingernails and toenails,and leukoplakia on the tongue.A histopathological study of the skin showed dyskeratocytes;however,a bone marrow biopsy revealed normal cell *** patient was diagnosed with dyskeratosis congenita,but her family history did not reveal significant ***-exome sequencing showed a novel heterozygous punctual mutation in exon 6 from the TINF2 gene,namely,NM_001099274.1:-c.854delp.(Val285-Alafs*32).An analysis of telomere length showed short telomeres relative to the patient’s *** The disease in this patient was caused by a germline novel mutation of TINF2 in one of her parents.

关键词： Dyskeratosis congenita TINF2 Germline mutation Novel mutation Short telomeres Case report

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人工牛黄甲硝唑胶囊致男性患者外生殖器多形红斑型药疹的诊疗分析

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抗感染药学 2024年第2期21卷 136-138页

作者：初丽娟吴涛王蓉华青岛大学附属威海市立第二医院山东威海264200

目的:分析1例人工牛黄甲硝唑胶囊致男性患者外生殖器多形红斑型药疹的诊疗过程,为临床该药的用药安全提供参考。方法与结果:该患者因牙痛而自行购买人工牛黄甲硝唑胶囊服用,但第2天患者发现外生殖器不行米粒至豆粒大小的红斑,呈密集对... 详细信息

目的:分析1例人工牛黄甲硝唑胶囊致男性患者外生殖器多形红斑型药疹的诊疗过程,为临床该药的用药安全提供参考。方法与结果:该患者因牙痛而自行购买人工牛黄甲硝唑胶囊服用,但第2天患者发现外生殖器不行米粒至豆粒大小的红斑,呈密集对称分布,伴轻微瘙痒,遂自行停药;但停药多日后仍未见好转,皮损还逐渐增大,且表面出现糜烂、渗出,遂入院治疗;入院后,对患者予以了抗炎、抗感染治疗,2周后患者红斑基本消失,1个月后患者皮损也基本痊愈;其间,医生采用诺氏评估量表考察多形红斑型药疹与人工牛黄甲硝唑胶囊的关联性,结果评分为6分,即关联性为“很可能”。结论:人工牛黄甲硝唑胶囊所致药疹仅出现在外生殖器部位而未涉及身体其他部位的情况较为罕见,临床在宣教时应做好用药交代,以便于患者早分辨、早治疗。

关键词：人工牛黄甲硝唑胶囊男性患者外生殖器多形红斑型药疹药物不良反应

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Historical relationships of areas of endemism of the Brazilian Atlantic rain forest： a cladistic biogeographic analysis of harvestman taxa （Arachnida： Opiliones）

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Current Zoology 2017年第5期63卷 525-535页

作者： Marcio B. DASILVA Ricardo PINTO-DA-ROCHA juan J. MORRONE Departamento de Sistematica e Ecologia CCEN Universidade Federal da Paraiba Cidade Universitaria Joao Pessoa PB Brazil Departamento de Zoologia Instituto de Biociencias Universidade de Sao Paulo Sao Paulo SP Brazil Museo de Zoologia "alfonso L. Herrera" Departamento de Biologia Evolutiva Facultad de Ciencias Universidad Nacional Autdnoma de Mexice (UNAM) 04510 Mexico City Mexico

Based on a cladistic biogeographic analysis of 6 species-level phylogenies of harvestman taxa, wesearched for congruence in the historical relationships of 12 areas of endemism of the BrazilianAtlantic Rain Forest. We constructed general area cladograms using Primary Brooks ParsimonyAnaJysis （BPA）, BPA of nodes, and paralogy-free subtree analysis. These analyses resulted in 6general area cladograms, that allow to infer a general pattern of the relationships among areas ofendemism from the Brazilian Atlantic Rain Forest. Northern areas resulted related basally showingmain disjunctions at the Doce River Valley and Todos os Santos Bay/S^o Francisco River *** remaining areas of endemism were included in a southern and a southeastern block, sepa-rated by the Ribeira do Iguape Valley. Incongruence Length Differences tests showed no significantincongruence among the resulting cladograms and other matrix partitions. We concluded that tec-tonism and ancient marine transgressions were the probable processes responsible for the maindisjunctions, whereas Neogene refugia seem to have caused the more recent disjunctions. Thegeneral pattern and redundancy in area relationships suggest a model of main reiterative barriersin diversification at multiple times for the evolution of the Atlantic Rain Forest. The renewal of cla-distic biogeography and the search for common biogeographic patterns are discussed.

关键词： dispersal, historical biogeography, Mata Atlantica, speciation, vicariance.

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HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium

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World Journal of Gastroenterology 2022年第29期28卷 3886-3902页

作者： Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama Laboratorio de Investigación en Enfermedades Infecciosas Hospital Infantil de México Federico GómezMexico City 06720Mexico Posgrado en Biomedicina y Biotecnología Molecular Laboratorio de Producción y Control de BiológicosEscuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalMexico City 11340Mexico Laboratorio de Adquisición de Hierro Universidad Autónoma de la Ciudad MéxicoPosgrado Ciencias GenómicaMexico City 03100Mexico Laboratorio Central de Microscopía Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalMexico City 11340Mexico Centro de estudios económicos y sociales en salud Hospital Infantil de México Federico GómezMexico City 06720Mexico Laboratorio de Investigación en Biología del Desarrollo y Teratogénesis Experimental Hospital Infantil de México Federico GómezMexico City 06720Mexico

BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence *** To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) *** Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional *** HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium *** technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS *** analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein

关键词： Hypothetical protein HP0953 Adherence Helicobacter pylori Glycocalyx Virulence factor Persistence

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Water Efflux in Red Blood Cells of Sickle Cell Patients under Spontaneous Deoxygenation

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Journal of Biosciences and Medicines 2016年第12期4卷 152-161页

作者： Manuel Arsenio Lores Guevara Yulianela Mengana Torres juan Carlos García naranjo Andrés Ramírez Aguilera Lidia Clara Suárez Beyrio María Altagracia Marichal Felue Teresa Simón Brada Jan Philippé Centro de Biofí sica Mé dica Santiago de Cuba Cuba Hospital General “juan Bruno Zayas alfonso” Santiago de Cuba Cuba Department of Clinical Chemistry Immunology and Microbiology Ghent University Ghent Belgium

The water transport through Red Blood Cells (RBC) membrane has been previously studied in Sickle Cell Disease (SCD) using oxygenated RBC or under complete deoxygenation. In this work, the water efflux in RBC of sickle cell patients was studied under spontaneous deoxygenation conditions. With that purpose, a magnetic resonance method was used to evaluate the water exchange time (τe) and the permeability through the erythrocyte membrane (P) measuring the spin-spin relaxation time (T2) in doped and non-doped RBC. Carr-Purcell-Meiboon-Gill (CPMG) pulse sequence was used to measure T2 in a magnetic resonance console coupled to one homogeneous magnet system (0.095 T). An increase of the water transport in RBC from sickle cell patients was observed and characterized with a τe value of 15.2 ± 0.8 ms. The abnormal activation of the Psickle, Gardos, and potassium chloride cotransporter channels starting from deoxygenation, as well as, the possible appearance of new pores due to the increase of the hemoglobin-membrane interaction, are suggested to explain this abnormal transport phenotype. The change of the water volume to surface ratio (V/S) in the sickle cells is also suggested to be considered in P calculation under deoxygenation. The results obtained in this work increase the fundamental knowledge about molecular mechanism involved in SCD and could be useful in the development of new methods for diagnostic and treatment evaluation.

关键词： Red Blood Cells Water Permeability Nuclear Magnetic Relaxation Sickle Cell Disease

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Gold nanoshells： Contrast agents for cell imaging by cardiovascular optical coherence tomography

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Nano Research 2018年第2期11卷 676-685页

作者： Jie Hu Francisco Sanz-Rodríguez Fernando Rivero Emma Martín Rodríguez Río Aguilar Torres Dirk H. Ortgies José García Solé Fernando alfonso Daniel Jaque Fluorescence Imaging Group Departamento de Física de Materiales Instituto Nicolás Cabrera Facultad de Ciencias Universidad Autónoma de Madrid Madrid Spain Instituto Ramón y Cajal de Investigación Sanitaria Hospital Ramón y Cajal Madrid Spain Departamento de Biología Universidad Autónoma de Madrid Madrid Spain Cardiology Department Hospital Universitario de la Princesa IIS-IP Universidad Autónoma de Madrid Madrid Spain

Optical coherence tomography （OCT） has gained considerable attention in interventional cardiovascular medicine and is currently used in clinical settings to assess atherosclerotic lesions and to optimize stent placement. Artery imaging at the cellular level constitutes the first step towards cardiovascular molecular imaging, which represents a major advance in the development of personalized noninvasive therapies. In this work we demonstrate that cardiovascular OCT can be used to detect individual cells suspended in biocompatible fluids. Importantly, the combination of this catheter-based clinical technique with gold nanoshells （GNSs） as intracellular contrast agents led to a substantial enhancement in the backscattered signal produced by individual cells. This cellular contrast enhancement was attributed to the large backscattering cross-section of GNSs at the OCT laser wavelength （1,300 nm）. A simple intensity analysis of OCT cross-sectional images of suspended cells makes it possible to identify the sub-population of living cells that successfully incorporated GNSs. The generalizability of this method was demonstrated using two different cell lines （HeLa and Jurkat cells）. This work provides novel insights into cardiovascular molecular imaging using specifically modified GNSs.

关键词： optical coherencetomography (OCT)intravascular imaging gold nanoparticles three-dimensional (3D)imaging

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