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Dual stimulus responsive borosilicate glass(BSG)scaffolds promote diabetic alveolar bone defectsrepair by modulating macrophage phenotype

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Bioactive Materials 2023年第8期26卷 231-248页

作者： Pengfei Tian limin Zhao jua.Kim Xian li Chunyu liu Xu Cui Tao liang Yunbo Du Xiehui Chen Haobo Pan Center for Human Tissues and Organs Degeneration Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055PR China Shenzhen Longhua District Central Hospital Shenzhen518000PR China Shenzhen Healthemes Biotechnology Co.Ltd Shenzhen518102PR China

The regeneration of alveolar bone is still clinical challenge,particularly accompanied with diabetes,causing metabolic disorder with a protracted low-grade inflammatory *** a result,the anticipated loading of biomaterials is highly suspicious in spontaneous modulation of cells function,which is mostly disturbed by constant *** this study,we developed glucose and hydrogen peroxide dual-responsive borosilicate glass(BSG)scaffolds loaded with epigallocatechin gallate(EGCG)to synergistically modulate the abnormal inflammation of diabetic alveolar bone *** was found that the release of EGCG by BSG could directly regulate the shift of macrophages from M1 to the M2 phenotype by promoting autophagy and lessening the inhibition of autophagic ***,EGCG can also indirectly regulate the polarization phenotype of macrophages by reducing the activation of NF-κb in stem cells and restoring its immunoregulatory ***,the addition of EGCG to BSG scaffold in diabetes allows for a more striking modulation of the macrophage phenotype in a timely *** altered macrophage phenotype reduces local inflammation and thus increases the ability to repair diabetic alveolar bone,showing promise for the treatment of alveolar defect in diabetic patients.

关键词： Borosilicate Epigallocatechin gallate(EGCG) Alveolar bone Autophagy Mitochondria Macrophage immunomodulatory Diabetes

Spontaneous immunomodulation and regulation of angiogenesis and osteogenesis by Sr/Cu-borosilicate glass (BSG) bone cement to repair critical bone defects

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生物活性材料(英文) 2023年第5期23卷 101-117页

作者： Shuaijie li liyan Zhang Chunyu liu jua.Kim Kun Su Tingli Chen limin Zhao Xiaomei Lu Hao Zhang Yinglin Cui Xu Cui Feng Yuan Haobo Pan Department of Orthopedics Tongji HospitalSchool of MedicineTongji UniversityShanghai200065China Center for Human Tissues and Organs Degeneration Shenzhen Institute of Advanced TechnologyChinese Academy of ScienceShenzhen518055China Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education and Tianjin College of BiotechnologyTianjin University of Science and Technology300457China Shenzhen Longhua District Central Hospital/the Affiliated Central Hospital of Shenzhen Longhua District Guangdong Medical UniversityShenzhen518110China Department of Orthopedics Shanxi Bethune HospitalTaiyuan030032China Shenzhen Key Laboratory of Marine Biomaterials Shenzhen Institute of Advanced TechnologyChinese Academy of ScienceShenzhen518055China

Injectable bone biomaterials like bone cement should be designed and fabricated with certain biological criteria,which include:1)recruitment and polarization of the macrophages from M1(pro-inflammatory)to M2(anti-inflammatory)phenotype,2)enhance vascularization,and 3)activate osteogenic differentiation of bone marrow-derived stem cells to promote bone *** far,no injectable biomaterials could spontaneously regulate the entire bone healing process that involves inflammation,angiogenesis,and ***,in this study,we designed bone cement comprised of strontium and copper-incorporated borosilicate glass(Sr/Cu-BSG)in the liquid phase of chitosan to modulate bone *** vitro studies showed that the controlled release of Sr and Cu ions up-regulated anti-inflammatory genes(IL-1Ra and TGF-β1)while down-regulating pro-inflammatory genes(IL-1βand IL-6)in macrophages at 3 *** and Cu ions also increased the expressions of angiogenic genes(VEGF and bFGF)in HUVECs at 5 days and osteogenic genes(Runx-2,OCN,and OPN)in hBMSCs at 7,14,and 21 days.5Sr3Cu-BSG bone cement exhibited the best anti-inflammatory,angiogenic,and osteogenic properties among the bone cement groups with different Sr and Cu ***-term and long-term implantation of Sr/Cu-BSGs in femoral condylar bone defects of rats and rabbits confirmed the in vitro results,where the degradation rate of Sr/Cu-BSG matched the bone healing *** to in vitro,the 5Sr3Cu-BSG group also showed the highest bone formation in *** physical and chemical properties,along with its bone repairing ability,make the Sr/Cu-BSG bone cement a good candidate biomaterial for treating bone defects.

关键词： Borosilicate glass bone cement Immunomodulation Angiogenesis Osteogenesis

酸枣仁皂苷A对抑郁小鼠行为及突触可塑性相关蛋白的作用

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现代食品科技 2024年第2期40卷 36-43页

作者：李会涛李剑男张桐贡济宇长春中医药大学药学院吉林长春130117

该文探究了酸枣仁皂苷A(Jujuboside A,jua.对抑郁模型小鼠行为及神经突触可塑性相关蛋白表达的作用。通过悬尾实验、强迫游泳实验、旷场实验、Morris水迷宫实验评价小鼠的抑郁状态,免疫组织化学染色法检测小鼠海马组织中脑源性神经营养... 详细信息

该文探究了酸枣仁皂苷A(Jujuboside A,jua.对抑郁模型小鼠行为及神经突触可塑性相关蛋白表达的作用。通过悬尾实验、强迫游泳实验、旷场实验、Morris水迷宫实验评价小鼠的抑郁状态,免疫组织化学染色法检测小鼠海马组织中脑源性神经营养因子(Brain Derived Neurotrophic Factor,BDNF)的表达水平,Western blot实验检测小鼠海马组织酪氨酸激酶受体B(Tyrosine Kinase Receptor B,TrkB)、cAMP反应元件结合蛋白(cAMP Responsive Element Binding,CREB)、突触后密度蛋白95(Postsynaptic Density Protein 95,PSD95)、自噬效应蛋白Beclin1(Autophagy Effector Protein Beclin1,Beclin1)的表达水平。结果表明,jua.著改善抑郁模型小鼠的抑郁状态。同时,jua.用后海马组织BDNF、TrkB、CREB、PSD95、Beclin1的表达水平较模型组分别上调了190.23%、137.24%、76.29%、169.32%、82.53%。综上所述,jua.抑郁模型小鼠具有显著的抗抑郁作用,并能明上调BDNF、TrkB、CREB、PSD95、Beclin1等神经突触可塑性相关蛋白的表达。实验结果为jua.抑郁症临床治疗方面的应用提供理论依据,并为探究抑郁症药物治疗靶点提供参考。

关键词：酸枣仁皂苷A 抑郁小鼠抗抑郁作用神经突触可塑性

Roadheader rear support system modeling and mechanical-hydraulic coupling system co-simulation research

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Roadheader rear support system modeling and mechanical-hydra...

Synthesis of potassium hexatitanate whiskers using hydrothermal method

第一届液压技术与矿山装备国际学术会议

作者： ZHAO li-jua.ZHAO Xin liaoning Technology University

In order to estimate the stability of the roadheader rear support system,took the heavy cantilever roadheader of a coal company as the research object,rigid-flex coupled dynamic model of its rear support system was established based on Pro/E,ADAMS and *** hydraulic control system model of rear support was researched in *** two models built the mechanical-hydraulic coupling *** the mechanical dynamic simulation results and verified the reliability of hydraulic system when the co-simulation of rear support was made.

关键词： rear support ADAMS EASY5 co-simulation

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Rare Metals 2009年第1期28卷 24-32页

作者： WANG Ju li Chun liANG Bin WANG Xiaoqing College of Chemical Engineering Siehuan University Chengdu 610065 China Department of Chemistry Tsinghua University Beijing 100084 China

High quality potassium hexatitanate whiskers were hydrothermally synthesized in one step under moderate temperature and pressure conditions. Effects of the titanium source and reaction conditions on the hydrothermal reaction rate, product phase component, and morphology of whiskers were investigated. The results show that the reactivity of hydrated titania, anatase TiO2, and rutile TiO2 with KOH decreases in turn, and with hydrated titania as titanium source, it is difficult to obtain potassium hexatitanate whiskers with good morphology. In contrast, uniform potassium hexatitanate whiskers with a length of 10-20 μm and a diameter of 200-700 nm were obtained using anatase TiO2 as titanium source. The investigation demonstrates that the initial KOH concentration, annealing temperature and time, molar ratio of K2O/TiO2, etc. significantly affect the morphology of the as-synthesized whiskers. The optimized synthesis condition is as follows： anatase as a titanium source 10 wt.% KOH solution; annealing temperature and time of 300℃ and 5 h, respectively; K2O/TiO2 molar ratio of 5, etc. A rhombic potassium hexatitanate was prepared under the optimum condition and the whisker grew along the [110] direction. The reaction mechanism was discussed.

关键词： potassium hexatitanate whiskers hydrothermal method synthesis

PD-1、FOXP3和CSF-1R蛋白表达对霍奇金淋巴瘤患者预后的影响

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中国实验血液学杂志 2018年第5期26卷 1372-1377页

作者：黎晓鹃何龙济南市第三人民医院肿瘤血液科山东济南250132

目的:研究集落刺激因子1受体(CSF-1R)、叉头转录因子3(FOXP3)和程序性死亡1(PD-1)蛋白表达对霍奇金淋巴瘤患者预后的影响。方法:记录本院收治的54例霍奇金淋巴瘤患者的临床特征资料和治疗方案。采集患者标本,采用免疫组织化学染色法检... 详细信息

目的:研究集落刺激因子1受体(CSF-1R)、叉头转录因子3(FOXP3)和程序性死亡1(PD-1)蛋白表达对霍奇金淋巴瘤患者预后的影响。方法:记录本院收治的54例霍奇金淋巴瘤患者的临床特征资料和治疗方案。采集患者标本,采用免疫组织化学染色法检测微环境相关的预后因子CSF-1R、FOXP3及PD-1蛋白表达,同时通过原位杂交技术检测EB病毒及其小编码的mRNA(EBER);探讨CSF-1R、FOXP3及PD-1 3种蛋白表达与霍奇金淋巴瘤患者预后的关系,并采用单因素分析和多因素分析(Cox比例风险模型)研究影响霍奇金淋巴瘤患者预后的相关因素。结果:54例霍奇金淋巴瘤患者中,CSF-1R^+22例(40. 74%),FOXP3高表达28例(51. 85%),PD-1^+9例(16. 67%)。单因素分析结果发现,国际预后指数(IPI)评分、EBER和FOXP3蛋白表达是霍奇金淋巴瘤患者无进展生存(PFS)的影响因素(均P <0. 05);临床分期(Ann Arbor分期)、IPI评分、EBER、CSF-1R和FOXP3蛋白表达是患者总生存(OS)的影响因素(均P <0. 05)。多因素分析(Cox比例风险模型)结果发现,EBER状态是霍奇金淋巴瘤患者PFS、OS的影响因素(P <0. 05); FOXP3蛋白表达是患者PFS的影响因素(P <0. 05); Ann Arbor分期、CSF-1R蛋白表达均是患者OS的影响因素(均P <0. 05)。结论:CSF-1R、FOXP3与霍奇金淋巴瘤患者的预后有密切联系,可为该病的靶向治疗提供一定的依据。

关键词：集落刺激因子1受体叉头转录因子3 程序性死亡1 霍奇金淋巴瘤

光谱法、量热法以及分子对接研究酸枣仁皂苷A与中心蛋白的作用

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食品安全质量检测学报 2024年第3期15卷 286-292页

作者：张文龙胡颖媛郭丽丽裴科山西中医药大学中药与食品工程学院晋中030619

目的研究酸枣仁皂苷A(jujuboside A,jua.与八肋游仆虫中心蛋白(Euplotes octocarinatus centrin,EoCen)的相互作用。方法采用圆二色光谱(circular dichroism spectroscopy,CD)以及三维荧光光谱研究EoCen的构象变化;采用稳态荧光光谱与等温滴定量热法(isothermal titration calorimetry,ITC)研究jua.EoCen作用的机制;分子对接技术模拟jua.EoCen的结合位点。结果jua.摩尔比1:1结合于EoCen的C端(二者反应的结合常数约为10^(5)L/mol),由ITC拟合结果可知,jua.EoCen复合物的形成是放热的过程,熵变对自由能的贡献较小,主要由焓变驱动。EoCen与jua.合后,构象发生变化,α-螺旋含量减少,酪氨酸残基的微环境变化,EoCen的C端不再结合蜂毒素。结论jua.要通过范德华力与EoCen结合,从而改变EoCen的构象,抑制其与靶蛋白的结合。

关键词：八肋游仆虫中心蛋白酸枣仁皂苷A 圆二色光谱三维荧光光谱稳态荧光光谱等温滴定量热法分子对接技术

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一例晚期肺癌脊柱转移患者的循证治疗

中国循证医学杂志 2013年第6期13卷 768-772页

作者：李娟石锐余萍四川省肿瘤医院肺部肿瘤病区成都610041 四川大学华西医院骨科成都610041

目的针对一例晚期肺癌脊柱转移患者病情和治疗预期,结合最新的循证医学证据,制定治疗决策。方法按照PICO原则,对患者在治疗中面临的问题进行转换并制定检索策略后,在多个数据库中进行文献检索。按照循证医学5级证据分级标准,选择最佳的... 详细信息

目的针对一例晚期肺癌脊柱转移患者病情和治疗预期,结合最新的循证医学证据,制定治疗决策。方法按照PICO原则,对患者在治疗中面临的问题进行转换并制定检索策略后,在多个数据库中进行文献检索。按照循证医学5级证据分级标准,选择最佳的临床证据进行解读,并指导治疗决策。结果检出相关文献148篇,最终纳入4篇系统评价/Meta分析。针对患者关心的4个方面的问题均获得1级证据支持,包括恢复脊髓功能(行走和括约肌功能)、局部疼痛控制、远期生存率和治疗并发症。根据患者意愿,最终选择了治疗后并发症相对高发,但能够更好恢复神经功能,显著缓解疼痛,提高远期生存率的手术减压治疗。术后患者行走功能完全恢复。结论采用循证治疗方法,能为肺癌脊柱转移伴脊髓压迫患者选择合理的治疗方案。对于恶性脊髓压迫患者,如出现行走功能障碍,且具备手术条件,应尽早进行减压手术,以尽早恢复脊髓功能,缓解疼痛,提高远期生存率。但医患双方均应对手术治疗存在较高的并发症风险有充分的共识和谅解。

关键词：肺癌脊柱转移循证治疗

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高龄慢性肾功能衰竭患者血液透析的临床探讨

国际移植与血液净化杂志 2009年第6期7卷 29-30页

作者：张锋陈丽君吴芳李彬宁夏医科大学第二附属医院肾内科

目的评估高龄肾功能衰竭患者血液透析的临床疗效。方法观察总结62例高龄肾功能衰竭患者血液透析治疗后的生存率和死亡原因,心脑血管并发症的发生情况及与透析充分性和营养状况相关的指标。结果高龄血液透析患者的长期存活率逐步提高,死... 详细信息

目的评估高龄肾功能衰竭患者血液透析的临床疗效。方法观察总结62例高龄肾功能衰竭患者血液透析治疗后的生存率和死亡原因,心脑血管并发症的发生情况及与透析充分性和营养状况相关的指标。结果高龄血液透析患者的长期存活率逐步提高,死亡原因主要为心血管疾病、感染及严重营养不良,常见心血管并发症为低血压。低白蛋白血症发生率仍明显高于非老年组。结论施行个体化的透析和治疗,积极改善营养,减少感染,有助于减少高龄透析患者的并发症发生和提高生存率。

关键词：血液透析高龄患者肾功能衰竭