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detection of Gram-Negative Bacteria Carrying the blaKPC-2 Gene from Mangrove Sediments

Journal of Pharmacy and Pharmacology 2019年第8期7卷 485-492页

作者： Wallace Ribeiro Nunes Neto Jessica Mayara Mendes Araujo Darlan Ferreira da Silva joveliane de melo monteiro Wallace Borges Pachêco Jessflan Rafael Nascimento Santos Maria Raimunda Chagas Silva Maria Rosa Quaresma Bomfim Andrea de Souza monteiro Laboratory of Applied Microbiology CEUMA UniversitySao Luis Maranhao 65000-000Brazil Laboratory of Environmental Sciences CEUMA UniversitySao Luis Maranhao 65000-000Brazil Laboratory of Molecular Biology Sciences CEUMA UniversitySao Luis Maranhao 65000-000Brazil

Mangroves are environments that have fast cycles associated with high concentration of bacterial *** are impacted by anthropogenic pollution due to contamination of bacterial species carrying resistance *** study aims to evaluate the metabolic profile of the microbiota in mangrove sediments,and verify the presence of Gram-negative bacteria resistant to *** samples were obtained from location along the Anil river and were seeded in medium supplemented with the antibiotic meropenem in increasing *** DNA was analyzed by multiplex PCR for detecting resistance genes forβ-lactam *** bacteria were identified by Matrix-assisted laser desorption ionization–time of flight mass spectrometry(MALDI-TOF MS).The genes were sequenced by ABI PRISM 3100,analyzed by MEGA 6.0 program,and the sequence identified was assessed by GenBank using the BLAST ***■the kit was used to determine the metabolic profile of the *** results of the six bacterial isolates showed the blaKPC-2 gene and were identified as Stenotrophomonas maltophilia and Pseudomonas *** samples showed a greater diversity Shannon index,a rich substrate consumption and high *** was a metabolic richness such as carbon consumption profiles,being a factor of adaptation of pathogenic bacteria carrying resistance genes toβ-lactamics antibiotics.

关键词： Antibiotic resistant meropenem bacterial isolates

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