Detection of Gram-Negative Bacteria Carrying the blaKPC-2 Gene from Mangrove Sediments
作者机构：Laboratory of Applied MicrobiologyCEUMA UniversitySao Luis Maranhao 65000-000Brazil Laboratory of Environmental SciencesCEUMA UniversitySao Luis Maranhao 65000-000Brazil Laboratory of Molecular Biology SciencesCEUMA UniversitySao Luis Maranhao 65000-000Brazil
出 版 物：《Journal of Pharmacy and Pharmacology》 (药剂与药理学（英文版）)
年 卷 期：2019年第7卷第8期
页 面：485-492页
学科分类：10[医学]
主 题：Antibiotic resistant meropenem bacterial isolates
摘 要：Mangroves are environments that have fast cycles associated with high concentration of bacterial *** are impacted by anthropogenic pollution due to contamination of bacterial species carrying resistance *** study aims to evaluate the metabolic profile of the microbiota in mangrove sediments,and verify the presence of Gram-negative bacteria resistant to *** samples were obtained from location along the Anil river and were seeded in medium supplemented with the antibiotic meropenem in increasing *** DNA was analyzed by multiplex PCR for detecting resistance genes forβ-lactam *** bacteria were identified by Matrix-assisted laser desorption ionization–time of flight mass spectrometry(MALDI-TOF MS).The genes were sequenced by ABI PRISM 3100,analyzed by MEGA 6.0 program,and the sequence identified was assessed by GenBank using the BLAST ***■the kit was used to determine the metabolic profile of the *** results of the six bacterial isolates showed the blaKPC-2 gene and were identified as Stenotrophomonas maltophilia and Pseudomonas *** samples showed a greater diversity Shannon index,a rich substrate consumption and high *** was a metabolic richness such as carbon consumption profiles,being a factor of adaptation of pathogenic bacteria carrying resistance genes toβ-lactamics antibiotics.
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