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Cellular & Molecular Immunology 2021年第4期18卷 1005-1015页

作者： Xuezhen Zeng Jingying Zhou Zhewen Xiong Hanyong Sun Weiqin Yang Myth T.S.Mok Jing Wang Jingqing Li Man Liu Wenshu Tang Yu feng Hector Kwong-Sang W ang Shun-Wa Tsang King-Lau Chow Philip Chun Yeung John Wong Paul Bo-San Lai Anthony Wing-Hung Chan Ka fai To Stephen Lam Chan Qiang Xia Jing Xue Xiao Chen Jun Yu Sui Peng joseph Jao-Yiu Sung Ming Kuang Alfred Sze-Lok Cheng School of Biomedical Sciences The Chinese University of Hong KongHong Kong SARChina lnstitute of Precision Medicine The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Department of Liver Surgery The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Department of Pharmacy The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Department of Anatomical and Cellular Pathology The Chinese University of Hong KongHong Kong SARChina Department of Liver Surgery Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Department of Gastroenterology The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Division of Life Science Hong Kong University of Science and TechnologyHong Kong SARChina Department of Surgery The Chinese University of Hong KongHong Kong SARChina State Key Laboratory of Translational Oncology The Chinese University of Hong KongHong Kong SARChina Department of Clinical Oncology The Chinese University of Hong KongHong Kong SARChina State Key Laboratory of Oncogenes and Related Genes Stem Cell Research CenterRen Ji HospitalSchool of MedicineShanghai Cancer InstituteShanghai Jiao Tong UniversityShanghaiChina Department of Medicine and Therapeutics The Chinese University of Hong KongHong Kong SARChina State Key Laboratory of Digestive Disease The Chinese University of Hong KongHong Kong SARChina Clinical Trial Unit The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Cancer Center The First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina

The liver is an immunologically tolerant organ and a common metastatic site of multiple cancer *** a role for cancer cell invasion programs has been well characterized,whether and how liver-intrinsic factors drive metastatic spread is incompletely ***,we show that aberrantly activated hepatocyte-intrinsic cell cycle-related kinase(CCRK)signaling in chronic liver diseases is critical for cancer metastasis by reprogramming an immunosuppressive *** an inducible liverspecific transgenic model,we found that CCRK overexpression dramatically increased both B16f10 melanoma and MC38 colorectal cancer(CRC)metastasis to the liver,which was highly infiltrated by polymorphonuclear-myeloid-derived suppressor cells(PMNMDSCs)and lacking natural killer T(NKT)*** of PMN-MDSCs in CCRK transgenic mice restored NKT cell levels and their interferon gamma production and reduced liver metastasis to 2.7% and 0.7%(metastatic tumor weights)in the melanoma and CRC models,***,CCRK activated nuclear factor-kappa B(Nf-κB)signaling to increase the PMN-MDSC trafficking chemokine C-X-C motif ligand 1(CXCL1),which was positively correlated with liver-infiltrating PMN-MDSC levels in CCRK transgenic ***,CRC liver metastasis patients exhibited hyperaaivation of hepatic CCRK/Nf-κB/CXCL1 signaling,which was associated with accumulation of PMN-MDSCs and paucity of NKT cells compared to healthy liver transplantation *** summary,this study demonstrates that immunosuppressive reprogramming by hepatic CCRK signaling undermines antimetastatic *** findings offer new mechanistic insights and therapeutic targets for liver metastasis intervention.

关键词： Cell cycle related kinase liver metastasis liver immune microenvironment myeloid-derived suppressor cell natural killer T cell

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变暖的世界

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作者： (美)威廉·诺德豪斯(William D. Nordhaus) 约瑟夫·博耶(joseph Boyer)

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Study of Biofilm formation and Antibiotic Resistance Pattern of Bacteria Isolated from Diabetic foot Ulcers in Hôpital de Référence Saint joseph, Kinshasa, Democratic Republic of Congo

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Advances in Microbiology 2021年第5期11卷 283-295页

作者： Jean-Marie Liesse Iyamba Victoire Marie Hermine Ngo Bassom Cyprien Mbundu Lukukula joseph Welo Unya Benjamin Kodondi Ngbandani Grégoire Mbusa Vihembo Nelson Nsiata Ngoma José Mulwahali Wambale Paul Tshilumbu Kantola N. B. Takaisi-Kikuni Laboratory of Experimental and Pharmaceutical Microbiology Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo Centre Universitaire de Référence de Surveillance de la Résistance aux Antimicrobiens Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo Service de Microbiologie H&ocirc pital de Référence Saint joseph Limete-Kinshasa Democratic Republic of Congo

foot infections resulting from biofilm producers and multi-drug resistant organisms is one of the most important complications of diabetes mellitus, as it can impede the wound healing process. This study was carried out in order to determine the antibiotic resistance pattern and the biofilm production in diabetic foot ulcers isolates. Clinical samples were collected from patients suffering from diabetic foot ulcers by using sterile swabs. Antibiotic susceptibility test was done using disk diffusion method on Mueller Hinton Agar. Biofilm formation was assessed by Crystal Violet Staining Method. Staphylococcus aureus isolates were resistant to ofloxacin (83.3%), ciprofloxacin (75.0%), trimethoprim-sulphamethoxazole (75.0%), and gentamicin (58.8%) but very sensitive to oxacillin (100.0%) and vancomycin (91.7%). Pseudomonas aeruginosa isolates showed resistance to the commonly used antibiotics such as ofloxacin, cefotaxime, ampicillin (81.8%), ceftazidime and imipenem (72.7%). The majority of bacteria studied were biofilm producers. This study showed that bacteria isolated from diabetic foot ulcers were biofilm producers and presented resistance to commonly used antibiotics. Knowledge on antibiotic sensitivity pattern and biofilm phenotype of the isolates will be helpful in determining the drugs for the treatment of diabetic ulcers.

关键词： Biofilm formation Antibiotic Resistance Diabetic foot Ulcers Democratic Republic of Congo

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止消通脉宁对转化生长因子-β_1诱导的人肾小管上皮细胞Ⅰ、Ⅲ型胶原及纤连蛋白mRNA表达的影响

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中国中医药信息杂志 2012年第11期19卷 33-35页

作者：张邦能杨丽霞程涛刘铜华吴丽丽 Margetts Peter joseph 甘肃省中医院甘肃省中医药研究院甘肃兰州730050 北京中医药大学北京100029 加拿大麦克马斯特大学

目的探讨止消通脉宁含药血清对转化生长因子-β1(TGf-β1)诱导的人肾小管上皮细胞(HK-2)Ⅰ型胶原(ColⅠ)、Ⅲ型胶原(ColⅢ)和纤连蛋白(fN)mRNA表达的影响。方法将HK-2细胞用含10%胎牛血清的DMEM/f12(1∶1)培养基培养,分为空白对照组、T... 详细信息

目的探讨止消通脉宁含药血清对转化生长因子-β1(TGf-β1)诱导的人肾小管上皮细胞(HK-2)Ⅰ型胶原(ColⅠ)、Ⅲ型胶原(ColⅢ)和纤连蛋白(fN)mRNA表达的影响。方法将HK-2细胞用含10%胎牛血清的DMEM/f12(1∶1)培养基培养,分为空白对照组、TGf-β1诱导组(TGf-β110 ng/mL)、空白血清对照组(TGf-β110 ng/mL+10%空白血清)、干预1组(TGf-β110 ng/mL+10%低剂量止消通脉宁含药血清)、干预2组(TGf-β110 ng/mL+10%中剂量止消通脉宁含药血清)、干预3组(TGf-β110 ng/mL+10%高剂量止消通脉宁含药血清)。药物干预24 h后,荧光定量PCR检测ColⅠ、ColⅢ和fN的mRNA表达。结果 HK-2细胞经TGf-β1诱导后,ColⅠ、ColⅢ和fN的mRNA表达显著上升,与空白对照组比较,差异有统计学意义(Pf-β1诱导组比较,差异有统计学意义(Pf-β1诱导的人肾小管上皮细胞ColⅠ、ColⅢ和fN的mRNA表达,减少细胞外基质成分的分泌,具有防治肾间质纤维化作用。

关键词：止消通脉宁转化生长因子-β1 人肾小管上皮细胞 Ⅰ型胶原 Ⅲ型胶原纤连蛋白

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止消通脉宁干预转化生长因子-β_1诱导人肾小管上皮细胞表型转化的研究

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中国中医药信息杂志 2013年第1期20卷 37-39,42页

作者：杨丽霞李晓东程涛刘铜华吴丽丽 Margetts Peter joseph 甘肃省中医药研究院甘肃兰州730050 北京中医药大学北京100029 加拿大麦克马斯特大学

目的观察止消通脉宁药物血清对转化生长因子-β1(TGf-β1)诱导人肾小管上皮细胞(HK-2)表型转化的影响,并探讨其机制。方法将HK-2细胞用含10%胎牛血清的DMEM/f12(1∶1)培养基培养并分为空白对照组、TGf-β1诱导组(TGf-β110 ng/mL)、空... 详细信息

目的观察止消通脉宁药物血清对转化生长因子-β1(TGf-β1)诱导人肾小管上皮细胞(HK-2)表型转化的影响,并探讨其机制。方法将HK-2细胞用含10%胎牛血清的DMEM/f12(1∶1)培养基培养并分为空白对照组、TGf-β1诱导组(TGf-β110 ng/mL)、空白血清对照组(TGf-β110 ng/mL+10%空白血清)、干预1组(TGf-β110 ng/mL+10%低剂量止消通脉宁药物血清)、干预2组(TGf-β110 ng/mL+10%中剂量止消通脉宁药物血清)、干预3组(TGf-β110 ng/mL+10%高剂量止消通脉宁药物血清)。药物干预24 h后,免疫组化检测α-平滑肌肌动蛋白(α-SMA)和E-钙黏蛋白(E-cadherin)的表达。在24、48、72 h应用酶联免疫吸附法检测细胞培养上清中Ⅰ型胶原(ColⅠ)、Ⅲ型胶原(ColⅢ)和纤连蛋白(fN)的含量。结果正常HK-2细胞表达E-cadherin,不表达α-SMA,经TGf-β1刺激后细胞表型发生转变,E-cadherin的表达明显减弱,α-SMA表达明显增强,且ColⅠ、ColⅢ和fN的分泌明显增加,与空白对照组比较差异有统计学意义(PfN的分泌,与TGf-β1诱导组比较差异有统计学意义(P<0.05)。结论止消通脉宁在一定程度上能够抑制人肾小管上皮细胞表型转化,减少细胞外基质成分的分泌,具有防治肾间质纤维化的作用。

关键词：止消通脉宁转化生长因子-β1 人肾小管上皮细胞表型转化 Ⅰ型胶原 Ⅲ型胶原纤连蛋白

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Potential factors influencing lymphatic filariasis transmission in"hotspot"and"control"areas in Ghana:the importance of vectors

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Infectious Diseases of Poverty 2019年第1期8卷 45-55页

作者： Sellase Pi-Bansa joseph Harold Nyarko Osei Kwadwo Kyeremeh frempong Elizabeth Elhassan Osei Kweku Akuoko David Agyemang Collins Ahorlu Maxwell Alexander Appawu Benjamin Guibehi Koudou Michael David Wilson Dziedzom Komi de Souza Samuel Kweku Dadzie Jurg Utzinger Daniel Adjei Boakye Swiss Tropical and Public Health Institute BaselSwitzerland University of Basel BaselSwitzerland Noguchi Memorial Institute for Medical Research College of Health SciencesUniversity of GhanaLegonGhana Department of Animal Biology and Conservation Science University of GhanaLegonGhana SightSavers International Ghana OfficeAccraGhana African Regional Postgraduate Programme in Insect Science University of GhanaLegonGhana Vector Biology Department Liverpool School of Tropical MedicineLiverpoolUK Centre Suisse de Recherches Scientifiques en Cote d'Ivoire AbidjanCote d'Ivoire

Background:Mass drug administration(MDA)programmes for the control of lymphatic filariasis in Ghana,have been ongoing in some endemic districts for 16 *** current study aimed to assess factors that govern the success of MDA programmes for breaking transmission of lymphatic filariasis in ***:The study was undertaken in two"hotspot"districts(Ahanta West and Kassena Nankana West)and two control districts(Mpohor and Bongo)in *** were collected and identified using morphological and molecular tools.A proportion of the cibarial armatures of each species was *** were performed onAnopheles gambiae for filarial worm detection.A questionnaire was administered to obtain information on MDA compliance and vector control *** were compared between districts to determine factors that might explain persistent transmission of lymphatic ***:High numbers of mosquitoes were sampled in Ahanta West district compared to Mpohor district(f=16.09,P=0.002).There was no significant difference between the numbers of mosquitoes collected in Kassena Nankana West and Bongo districts(f=2.16,P=0.185).Mansonia species were predominant in Ahanta West *** mosquitoes were prevalent in all *** with infected and infective filarial worms was found only in Ahanta West *** differences were found in cibarial teeth numbers and shape for mosquito species in the surveyed *** MDA coverage was high in all *** average use of bednet and indoor residual spraying was 82.4 and 66.2%,*** was high compliance in the five preceding MDA rounds in Ahanta West and Kassena Nankana West districts,both considered hotspots of lymphatic filariasis ***:The study on persistent transmission of lymphatic filariasis in the two areas in Ghana present information that shows the importance of local understanding of factors affecting control and elimination of lymphatic

关键词： Ghana Hotspots Lymphatic filariasis Mass drug administration Microfilariae Systematic non-compliance Vector control

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低压大流量热式气体质量流量计分流测试方法研究

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计量科学与技术 2022年第8期66卷 3-6,12页

作者：黎荣发凌光盛赵豪张富源谢代梁徐雅卓度计量技术(深圳)有限公司深圳518116 中国计量大学杭州310018

热式气体质量流量计具有量程比大、精度高以及可靠性好等优点,可广泛应用于工业天然气、家用煤气以及医疗中氧气等气体流量的测量,在工业以及民用领域中有着广阔的应用前景。目前国内市场上低压天然气实流测试标准装置的最大测试流量通... 详细信息

热式气体质量流量计具有量程比大、精度高以及可靠性好等优点,可广泛应用于工业天然气、家用煤气以及医疗中氧气等气体流量的测量,在工业以及民用领域中有着广阔的应用前景。目前国内市场上低压天然气实流测试标准装置的最大测试流量通常只能达到40 m^(3)·h^(-1),无法对低压大流量气体质量流量计进行实流测试,给生产上带来很多不便。本文利用分流组件对热式气体质量流量计进行改装,通过数值模拟对分流组件与整表的流场特性进行了对比验证。采用实流测试设备对该分流组件进行实流测试,利用计算获得的流量比例系数获得流量计整表的流量性能,从而解决了低压天然气大流量气体质量流量计的实流测试问题。

关键词：气体质量流量计分流组件实流测试流量比例系数数值模拟

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东亚奇迹的反思

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作者： (Stiglitz joseph E.)斯蒂格利茨 (Yusuf Shahid)尤素福

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山东省临朐县胃癌高发现场的流行病学与预防研究

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中华预防医学杂志 1999年第Z1期33卷 54-54页

作者：游伟程昌云生张联马峻岭刘伟东金懋林李吉友 William J Blot Mitchell H Gail joseph f fraumeni Jr 徐光炜北京市肿瘤防治研究所北京医科大学临床肿瘤学院山东省临朐县卫生局美国国立癌症研究所

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糖耐康对转化生长因子-β_1诱导的HK-2细胞分泌细胞外基质成分的影响

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中国中医药信息杂志 2013年第8期20卷 39-41页

作者：李钦杨丽霞张邦能刘铜华吴丽丽孙文 Margetts Peter joseph 甘肃省中医学校甘肃兰州730050 甘肃省中医院甘肃省中医药研究院甘肃兰州730050 北京中医药大学北京100029 麦克马斯特大学

目的观察糖耐康对转化生长因子-β1(TGf-β1)诱导的人肾小管上皮细胞(HK-2)对细胞外基质成分表达的影响,探讨糖耐康治疗肾间质纤维化的作用机制。方法将HK-2细胞用含10%胎牛血清的DMEM/f12(1∶1)培养基培养,并分为6组:空白对照组、TGf-... 详细信息

目的观察糖耐康对转化生长因子-β1(TGf-β1)诱导的人肾小管上皮细胞(HK-2)对细胞外基质成分表达的影响,探讨糖耐康治疗肾间质纤维化的作用机制。方法将HK-2细胞用含10%胎牛血清的DMEM/f12(1∶1)培养基培养,并分为6组:空白对照组、TGf-β1诱导组(TGf-β110 ng/mL)、空白血清对照组(TGf-β110 ng/mL+10%空白血清)、干预1组(TGf-β110 ng/mL+5%糖耐康药物血清)、干预2组(TGf-β110 ng/mL+10%糖耐康药物血清)、干预3组(TGf-β110 ng/mL+20%糖耐康药物血清)。药物干预24 h,荧光定量PCR检测Ⅰ型胶原(ColⅠ)、Ⅲ型胶原(ColⅢ)和纤连蛋白(fN)的mRNA表达。结果 HK-2细胞经TGf-β1诱导后,ColⅠ、ColⅢ和fN的mRNA表达显著上升,与空白对照组比较,差异有统计学意义(Pf-β1诱导组比较,差异有统计学意义(Pf-β1诱导的人肾小管上皮细胞ColⅠ、ColⅢ和fN的mRNA表达,减少细胞外基质成分的分泌,具有防治肾间质纤维化的作用。

关键词：糖耐康转化生长因子-β1 HK-2细胞 Ⅰ型胶原 Ⅲ型胶原纤连蛋白

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