Study of Biofilm Formation and Antibiotic Resistance Pattern of Bacteria Isolated from Diabetic Foot Ulcers in Hôpital de Référence Saint Joseph, Kinshasa, Democratic Republic of Congo

作     者：Jean-Marie Liesse Iyamba Victoire Marie Hermine Ngo Bassom Cyprien Mbundu Lukukula Joseph Welo Unya Benjamin Kodondi Ngbandani Grégoire Mbusa Vihembo Nelson Nsiata Ngoma José Mulwahali Wambale Paul Tshilumbu Kantola N. B. Takaisi-Kikuni Jean-Marie Liesse Iyamba;Victoire Marie Hermine Ngo Bassom;Cyprien Mbundu Lukukula;Joseph Welo Unya;Benjamin Kodondi Ngbandani;Grégoire Mbusa Vihembo;Nelson Nsiata Ngoma;José Mulwahali Wambale;Paul Tshilumbu Kantola;N. B. Takaisi-Kikuni

作者机构：Laboratory of Experimental and Pharmaceutical Microbiology Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo Centre Universitaire de Référence de Surveillance de la Résistance aux Antimicrobiens Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo Service de Microbiologie Hô pital de Référence Saint Joseph Limete-Kinshasa Democratic Republic of Congo 

出 版 物：《Advances in Microbiology》 (微生物学（英文）)

年 卷 期：2021年第11卷第5期

页      面：283-295页

学科分类：0202[经济学-应用经济学] 02[经济学] 020205[经济学-产业经济学] 

主　　题：Biofilm Formation Antibiotic Resistance Diabetic Foot Ulcers Democratic Republic of Congo 

摘      要：Foot infections resulting from biofilm producers and multi-drug resistant organisms is one of the most important complications of diabetes mellitus, as it can impede the wound healing process. This study was carried out in order to determine the antibiotic resistance pattern and the biofilm production in diabetic foot ulcers isolates. Clinical samples were collected from patients suffering from diabetic foot ulcers by using sterile swabs. Antibiotic susceptibility test was done using disk diffusion method on Mueller Hinton Agar. Biofilm formation was assessed by Crystal Violet Staining Method. Staphylococcus aureus isolates were resistant to ofloxacin (83.3%), ciprofloxacin (75.0%), trimethoprim-sulphamethoxazole (75.0%), and gentamicin (58.8%) but very sensitive to oxacillin (100.0%) and vancomycin (91.7%). Pseudomonas aeruginosa isolates showed resistance to the commonly used antibiotics such as ofloxacin, cefotaxime, ampicillin (81.8%), ceftazidime and imipenem (72.7%). The majority of bacteria studied were biofilm producers. This study showed that bacteria isolated from diabetic foot ulcers were biofilm producers and presented resistance to commonly used antibiotics. Knowledge on antibiotic sensitivity pattern and biofilm phenotype of the isolates will be helpful in determining the drugs for the treatment of diabetic ulcers.