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A method to deliver patterned electrical impulses to Schwann cells cultured on an artificial axon

Neural Regeneration Research 2019年第6期14卷 1052-1059页

作者： Antonio merolli Yong mao Gregory Voronin joseph a.m.steele N.Sanjeeva murthy Joachim Kohn New Jersey Center for Biomaterials Rutgers-The State University of New Jersey In Vivo Research Services Rutgers-The State University of New Jersey

Information from the brain travels back and forth along peripheral nerves in the form of electrical impulses generated by neurons and these impulses have repetitive patterns. Schwann cells in peripheral nerves receive molecular signals from axons to coordinate the process of myelination. There is evidence, however,that non-molecular signals play an important role in myelination in the form of patterned electrical impulses generated by neuronal activity. The role of patterned electrical impulses has been investigated in the literature using co-cultures of neurons and myelinating cells. The co-culturing method, however, prevents the uncoupling of the direct effect of patterned electrical impulses on myelinating cells from the indirect effect mediated by neurons. To uncouple these effects and focus on the direct response of Schwann cells,we developed an in vitro model where an electroconductive carbon fiber acts as an artificial axon. The fiber provides only the biophysical characteristics of an axon but does not contribute any molecular *** our "suspended wire model", the carbon fiber is suspended in a liquid media supported by a 3D printed scaffold. Patterned electrical impulses are generated by an Arduino 101 microcontroller. In this study, we describe the technology needed to set-up and eventually replicate this model. We also report on our initial in vitro tests where we were able to document the adherence and ensheath of human Schwann cells to the carbon fiber in the presence of patterned electrical impulses(hSCs were purchased from ScienCell Research Laboratories, Carlsbad, CA, USA; ScienCell fulfills the ethic requirements, including donor's consent). This technology will likely make feasible to investigate the response of Schwann cells to patterned electrical impulses in the future.

关键词： Schwann cell carbon fiber myelination electrical impulse artificial axon in vitro system Arduino microcontroller myelin basic protein

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Comparison of methods for deriving phenotypes from incomplete observation data with an application to age at puberty in dairy cattle

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Journal of Animal Science and Biotechnology 2024年第2期15卷 535-545页

作者： melissa A.Stephen Chris R.Burke Jennie E.Pryce Nicole m.steele Peter R.Amer Susanne meier Claire V.C.Phyn Dorian J.Garrick DairyNZ Ltd 605 Ruakura RoadHamilton 3240New Zealand AL Rae Centre for Genetics and Breeding-massey University RuakuraHamilton 3214New Zealand Agriculture Victoria Research AgriBioCentre for AgriBioscienceBundooraVictoria 3083Australia School of Applied Systems Biology La Trobe UniversityBundooraVictoria 3083Australia AbacusBio DunedinNew Zealand

Background many phenotypes in animal breeding are derived from incomplete measures,especially if they are challenging or expensive to measure *** include time-dependent traits such as reproductive status,or *** measures for these traits result in phenotypes that are subject to left-,interval-and rightcensoring,where phenotypes are only known to fall below an upper bound,between a lower and upper bound,or above a lower bound *** we compare three methods for deriving phenotypes from incomplete data using age at first elevation(>1 ng/mL)in blood plasma progesterone(AGEP4),which generally coincides with onset of puberty,as an example *** We produced AGEP4 phenotypes from three blood samples collected at about 30-day intervals from approximately 5,000 Holstein–Friesian or Holstein–Friesian×Jersey cross-bred dairy heifers managed in 54 seasonal-calving,pasture-based herds in New *** used these actual data to simulate 7 different visit scenarios,increasing the extent of censoring by disregarding data from one or two of the three *** methods for deriving phenotypes from these data were explored:1)ordinal categorical variables which were analysed using categorical threshold analysis;2)continuous variables,with a penalty of 31 d assigned to right-censored phenotypes;and 3)continuous variables,sampled from within a lower and upper bound using a data augmentation *** Credibility intervals for heritability estimations overlapped across all methods and visit scenarios,but estimated heritabilities tended to be higher when left censoring was *** sires with at least 5 daughters,the correlations between estimated breeding values(EBVs)from our three-visit scenario and each reduced data scenario varied by method,ranging from 0.65 to *** estimated breed effects also varied by method,but breed differences were smaller as phenotype censoring *** Our results indicate that using

关键词： Cattle Gibbs sampler markov-chain monte Carlo(mCmC) Puberty

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Intensity of effort and momentary failure in resistance training:Are we asking a binary question for a continuous variable?

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Journal of Sport and Health Science 2022年第6期11卷 644-647页

作者： James P.Fisher James steele Dave Smith School of Sport Health and Social ScienceSolent UniversitySouthamptonSO140YNUK Research Centre for musculoskeletal and Sports medicine Manchester Metropolitan UniversityManchesterM156BHUK

*** There is a growing body of research supporting the idea that a plethora of health benefits can result from resistance training using a low volume,high-effort approach(e.g.,increased metabolic rate and bone mineral density,a reduction in blood pressure,and improved muscle quality and insulin sensitivity,among ***,muscular strength and muscle massprimary goals and benefits of participation in resistance trainingare independently strong predictors of longevity and quality of life. Thus,many have sought to identify how the manipulation of resistance training variables might lead to“optimal”adaptations in such outcomes,as evidenced by repeated attempts over recent decades to review the literature and provide consensus statements on this topic.5-10 One variable that is often hotly debated within resistance training is training to failure.

关键词： resistance binary continuous

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Environmental variation shifts the relationship between trees and scatterhoarders along the continuum from mutualism to antagonism

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Integrative Zoology 2018年第3期13卷 319-330页

作者： Gina m.SAWAYA Adam S.GOLDBERG michael A.steele Harmony J.DALGLEISH Department of Biology College of William and MaryWilliamsburgVirginiaUSA Department of Biology Wilkes UniversityWilkes-BarrePennsylvaniaUSA

The conditional mutualism between scatterhoarders and trees varies on a continuum from mutualism to antagonism and can change across time and space,and among *** examined 4 tree species(red oak[Quercus rubra],white oak[Quercus alba],American chestnut[Castanea dentata]and hybrid chestnut[***×Castanea] mollissima)across 5 sites and 3 years to quantify the variability in this conditional *** used a published model to compare the rates of seed emergence with and without burial to the probability that seeds will be cached and left uneaten by scatterhoarders to quantify variation in the conditional mutualism that can be explained by environmental variation among sites,years,species,and seed provenance within *** species tested had increased emergence when ***,comparing benefits of burial to the probability of caching by scatterhoarders indicated a mutualism in red oak,while white oak was nearly always *** was variable around the boundary between mutualism and antagonism,indicating a high degree of context dependence in the relationship with *** found that different seed provenances did not vary in their potential for *** did not explain microsite differences in seed emergence in any of the species *** hybrid chestnut only,emergence on the surface declined with soil moisture in the *** quantifying the variation in the conditional mutualism that was not caused by changes in scatterhoarder behavior,we show that environmental conditions and seed traits are an important and underappreciated component of the variation in the relationship between trees and scatterhoarders.

关键词： Castanea dentata conditional mutualism Quercus alba Quercus rubra temperate forest

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Invasive oaks escape pre-dispersal insect seed predation and trap enemies in their seeds

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Integrative Zoology 2018年第3期13卷 228-237页

作者： michałBOGDZIEWICZ Raul BONAL Josep mESPELTA Ewa m.KALEmBA michael A.steele RafałZWOLAK Department of Systematic Zoology Faculty of BiologyAdam Mickiewicz UniversityPoznańPoland CREAF Cerdanyola del VallesCataloniaSpain Forest Research Group INDEHESAUniversity of ExtremaduraPlasenciaSpain DITEG Research Group University of Castilla-La ManchaToledoSpain Laboratory of Seed Biochemistry Institute of DendrologyPolish Academy of SciencesKórnikPoland Department of Biology Wilkes UniversityWilkes-BarrePennsylvaniaUSA

Species introduced to habitats outside their native range often escape control by their natural *** competing with native species,an alien species might also affect the native herbivores by introducing a new source of different quality ***,we describe the case of northern red oak(Quercus rubra)invasion in *** collected data on insect(moth Cydia *** weevil Curculio spp.)seed predation of northern red oak in its native(USA,North America)and invasive(Poland,Europe)range,as well as for sessile oaks(Quercus petrea)in *** also evaluated the quality of acorns as hosts for weevil larvae by collecting infested acorns and measuring weevil developmental success,and quantifying acorn traits such as seed mass,tannins,lipids and protein *** used DNA barcoding to identify insects to the species *** predation by moths was similar and very low in both species and in both ***,red oaks escape pre-dispersal seed predation by weevils in *** infestation rates of northern red oak acorns in their invasive range were 10 times lower than that of sessile oaks,and also 10 times lower than that of red oaks in North ***,even when weevils oviposited into northern red oaks,the larvae failed to develop,suggesting that the exotic host created a trap for the *** phenomenon might gradually decrease the local abundance of the seed predator,and further aid the invasion.

关键词： biotic invasions ecological trap enemy release hypothesis seed predation weevils

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Neurosurgical treatment for addiction: lessons from an untold story in China and a path forward

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National Science Review 2020年第3期7卷 702-712页

作者： Shuo ma Chencheng Zhang Ti-fei Yuan Douglas steele Valerie Voon Bomin Sun Department of Functional Neurosurgery Ruijin Hospital Shanghai Jiao Tong University School of Medicine Shanghai Key Laboratory of Psychotic Disorders Shanghai Mental Health CenterShanghai Jiao Tong University School of Medicine Co-innovation Center of Neuroregeneration Nantong University Division of Imaging Science and Technology Medical School University of Dundee Department of Psychiatry University of Cambridge

Addiction is a major public-health crisis associated with significant disability and mortality. Although various pharmacological and behavioral treatments are currently available, the clinical efficacy of these treatments is limited. Given this situation, there is a growing interest in finding an effective neurosurgical treatment for addiction. First, we discuss the use of ablative surgery in treating addiction. We focus on the rise and fall of nucleus accumbens ablation for addiction in China. Subsequently, we review recent studies that have explored the efficacy and safety of deep-brain-stimulation treatment for addiction. We conclude that neurosurgical procedures, particularly deep-brain stimulation, have a potentially valuable role in the management of otherwise intractable addictive disorders. Larger well-controlled clinical trials, however, are needed to assess clinical efficacy and safety. We end by discussing several key issues involved in this clinical field and identifying some areas of progress.

关键词： drug addiction psychosurgery ablative surgery deep-brain stimulation medical ethics

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A mixed-studies systematic review and meta-analysis of school-based interventions to promote physical activity and/or reduce sedentary time in children

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Journal of Sport and Health Science 2020年第1期9卷 3-17,102页

作者： michelle Jones Emmanuel Defever Ayland Letsinger James steele Kelly A mackintosh Research Office Plymouth Marjon UniversityPlymouth PL48AAUK School of Sport Health and Social SciencesSolent UniversitySouthamptonSO14 OYNUK Department of Health and Kinesiology Texas A&M UniversityCollege StationTX 77843USA The ukactive Research Institute LondonWC1R 4HEUK Applied Sports Science Swansea UniversitySwanseaSA28PPUK

Purpose:The aim of this mixed-studies systematic review was to ascertain the effectiveness of school-based interventions in increasing physical activity(PA) and/or reducing sedentary time(ST) in children aged 5-11 years,as well as to explore their effectiveness in relation to categories of the theory of expanded,extended,and enhanced opportunity(TEO).methods:Adhering to the Preferred Reporting Items for Systematic Reviews and meta-Analyses(PRISmA) guidelines,5 databases were searched using predefined search *** title and abstract screening of 1115 records,the removal of duplicates(n=584) and articles that did not meet the inclusion criteria agreed to a priori(n=419) resulted in 112 records that were full-text *** independent reviewers subsequently used the mixed-methods appraisal tool to assess the methodological quality of 57 full-text studies that met the inclusion criteria after full-text *** interventions were summarised using the TIDierR checklist and *** strength of evidence was determined using a 5-level rating system utilising a published decision ***:Overall evidence ratings for interventions implemented within school settings were:no evidence of effects on moderate-to-vigorous physical activity(mVPA) and inconclusive evidence of effects on sedentary *** relation to the TEO,expansion of PA appeared to be the most promising intervention type for mVPA,with moderate evidence of effect,whereas extension and enhancement of PA opportunity demonstrated no evidence of effect.A critical issue of possible compensatory behavior was identified by analysis of intervention effect in relation to PA measurement duration;when studies measured changes in PA during the actual intervention,there was moderate evidence of effect,whereas those that measured changes in PA during the school day presented inconclusive evidence of effect,and those that measured changes in PA over a whole day yielded no evidence of *** meta-analys

关键词： Children Intervention Physical activity School Sedentary time

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Design and Characterization of an Aerosol Test Chamber for Emergency Response Patient Contamination Control Simulation and Research

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Occupational Diseases and Environmental medicine 2023年第1期11卷 78-96页

作者： megan L. steele Emily m. Spatz George P. Lemmer Jacob m. Denney Jeremy m. Slagley Casey W. Cooper Robert m. Eninger Fenic Ltd. Dayton USA Department of Systems Engineering and management Air Force Institute of Technology Wright-Patterson Air Force Base Montgomery County USA Air Force materiel Command Office of the Command Surgeon Wright-Patterson Air Force Base Montgomery County USA

Contaminated or infected patients present a risk of cross-contamination for emergency responders, attending medical personnel and medical facilities as they enter a treatment facility. The controlled conditions of an aerosol test chamber are required to examine factors of contamination, decontamination, and cross-contamination. This study presents the design, construction, and a method for characterizing an aerosol test chamber for a full-sized manikin on a standard North Atlantic Treaty Organization litter. The methodology combined air velocity measurements, aerosol particle counts and size distributions, and computational fluid dynamics modeling to describe the chamber’s performance in three dimensions. This detailed characterization facilitates future experimental design by predicting chamber performance for a variety of patient-focused research.

关键词： Test Chamber Characterization Aerosol Generation Exposure Chamber Design Air Velocity mapping Spatial Variability CFD modeling Patient Decontamination

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吞咽筛查证据总结：对中国开展吞咽障碍康复实践的思考

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中华物理医学与康复杂志 2011年第12期33卷 946-948页

作者： Catriona.m.steele 加拿大大学健康网络多伦多康复研究所；加拿大多伦多大学言语-语言病理学系加拿大

吞咽障碍是指吞咽困难，病因可能为神经损伤或疾病，或者头颈的先天性、结构性或手术后改变，如头颈部癌治疗后。吞咽障碍的诊断和干预相对来说是一个新兴领域，

关键词：吞咽障碍康复中国证据筛查头颈部癌吞咽困难神经损伤

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Syringoid eccrine carcinoma of the eyelid presenting as cicatricial entropion

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International Journal of Ophthalmology(English edition) 2014年第5期7卷 912-913页

作者： manjool m.Shah Eric A.steele Kevin P.White David J.Wilson Department of Ophthalmology Casey Eye InstituteOregon Health&Science University Portland Veteran's Affairs medical Center Department of Dermatology Oregon Health&Science University

Dear Sir,my name is manjool ***,from the Casey Eye Institute at Oregon Health&Science University in Portland,Oregon,USA.I wish to write to you with regards to an interesting presentation of a rare eyelid malignancy.A ... 详细信息

关键词： mICROCYSTIC ADNEXAL CARCINOmA SYRINGOmATOUS CARCINOmA ORBIT

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