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Nutritional value of a partially defatted and a highly defatted black soldier fly larvae (Hermetia illucens L.) meal for broiler chickens: apparent nutrient digestibility,apparent metabolizable energy and apparent ileal amino acid digestibility

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Journal of Animal Science and Biotechnology 2017年第4期8卷 897-905页

作者： Achille Schiavone Michele De Marco Silvia Martínez Sihem Dabbou Manuela Renna josefa madrid Fuensanta Hernandez Luca Rotolo Pierluca Costa Francesco Gai Laura Gasco Department of Veterinary Sciences University of Turin Largo Paolo Braccini 2 10095 Grugliasco TO Italy Department of Animal Production University of Murcia Campus de Espinardo 30071 Murcia Spain Department of Agricultural Forest and Food Sciences University of Turin Largo Paolo Braccini 2 10095 Grugliasco TO Italy Institute of Science of Food Production National Research Council Largo Paolo Braccini 2 10095 Grugliasco TO Italy

Background: The study aimed to determine the apparent total tract digestibility coefficients(ATTDC) of nutrients, the apparent metabolizable energy(AME and AMEn) and the amino acid(AA) apparent ileal digestibility coefficients(AIDC)of a partially defatted(BSFp) and a highly defatted(BSFh) black soldier fly larvae meal. The experimental diets were: a basal diet and two diets prepared by substituting 250 g/kg(w/w) of the basal diet with BSFp or BSFh, ***: Significant differences were found between BSFp and BSFh meals for ATTDC of the nutrients: BSFp resulted more digestible than BSFh, except for ATTDC of CP which did not differed between meals, while a statistical trend was observed for ATTDC of DM and EE. The AME and AMEn values were significantly(P < 0.05) different between the two BSF meals, with higher levels for BSFp(16.25 and 14.87 MJ/kg DM, respectively). The AIDC of the AA in BSFp ranged from 0.44 to 0.92, while in BSFh they ranged from 0.45 to 0.99. No significant differences were observed for the AA digestibility(0.77 and 0.80 for BSFp and BSFh, respectively), except for glutamic acid, proline and serine that were more digestible in the BSFh meal(P < 0.05).Conclusions: Defatted BSF meals can be considered as an excellent source of AME and digestible AA for broilers with a better efficient nutrient digestion. These considerations suggested the effective utilization of defatted BSF larvae meal in poultry feed formulation.

关键词： Amino acid Apparent digestibility Black soldier fly meal Broiler chicken Metabolizable energy

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Nutritional effects of the dietary inclusion of partially defatted Hermetia illucens larva meal in Muscovy duck

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Journal of Animal Science and Biotechnology 2019年第3期10卷 768-777页

作者： Marta Gariglio Sihem Dabbou Ilaria Biasato Maria Teresa Capucchio Elena Colombino Fuensanta Hernandez josefa madrid Silvia Martinez Francesco Gai Christian Caimi Sara Bellezza Oddon Marco Meneguz Angela Trocino Riccardo Vincenzi Laura Gasco Achille Schiavone Department of Veterinary Sciences University of Turinlargo Paolo Braccini 2TurinGrugliasco 10095Italy Department of Agricultural Forest and Food SciencesUniversity of Turinlargo Paolo Braccini 2TurinGrugliasco 10095Italy Department of Animal Production University of MurciaCampus de Espinardo30071 MurciaSpain Institute of Science of Food Production National Research Councillargo Paolo Braccini 2TurinGrugliasco 10095Italy Department of Comparative Biomedicine and Food Science University of Paduaviale dell’Universita 16PaduaLegnaro 35020Italy A.I.A.Agricola Italiana Alimentare S.p.A via Val Pantena 18G37142 VeronaItaly 7Institute of Interdisciplinary Research on Sustainability University of Turinvia Accademia Albertina 1310100 TurinItaly

Background: The present work is aimed at evaluating the effect of different inclusion levels of a partially defatted black soldier fly(Hermetia illucens,L.;HI) larva meal for ducks.A total of 192 female 3-day-old Muscovy ducklings(Cairina moschata domestica,Canedins R71 L White,Grimaud Freres Selection,France) were divided into 4 groups,assigned 4 different dietary treatments(6 replicates/treatment and 8 birds/replicate) and reared from 3 to 50 days of *** larva meal was included at increasing levels(0,3%,6% and 9%,HI0,HI3,HI6 and HI9,respectively) in isonitrogenous and isoenergetic diets formulated for 3 feeding phases: starter(3–17 days of age),grower(18–38 days of age) and finisher(39–50 days of age).The growth performance and apparent total tract digestibility were evaluated during the trial using titanium dioxide as an inert marker(0.3% of inclusion).At 51 days of age,two birds per pen were slaughtered and histomorphological investigations were ***: The live weight and average daily gain showed a quadratic response to increasing HI meal in the grower period(minimum corresponding to the HI6 group).No effects of dietary inclusion levels were observed for the daily feed intake or feed conversion *** apparent dry matter and organic matter digestibility were not affected by the dietary treatment.A linear decrease was observed for the crude protein apparent digestibility in the starter period(minimum for the HI9 groups).The ether extract apparent digestibility increased linearly during the grower and finisher periods(minimum for the HI0 group).The morphometric indices were not influenced by the dietary ***: The inclusion of up to 9% of HI partially defatted larva meal in the diet of ducks did not cause any effect on growth performance,as well as the apparent ***,dietary HI inclusion preserved the physiological intestinal development.

关键词： Black soldier fly Digestibility Ducks Histopathology Insect Performance

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Nutritional effects of the dietary inclusion of partially defatted Hermetia illucens larva meal in Muscovy duck

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Journal of Animal Science and Biotechnology 2019年第3期

作者： Marta Gariglio Sihem Dabbou Ilaria Biasato Maria Teresa Capucchio Elena Colombino Fuensanta Hernández josefa madrid Silvia Martínez Francesco Gai Christian Caimi Sara Bellezza Oddon Marco Meneguz Angela Trocino Riccardo Vincenzi Laura Gasco Achille Schiavone

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Maternal Diaphragmatic Hernia Correction During Pregnancy

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Maternal-Fetal Medicine 2023年第4期5卷 257-259页

作者： Juan Sebastián Barona-Wiedmann Mauricio Velásquez Maria josefa Franco Henry Muñoz Albaro José Nieto-Calvache Postgrado en Ginecología y Obstetricia Universidad IcesiFacultad de Ciencias de la SaludCali 760032Colombia Departamento de Cirugía General Fundación Valle del LiliCali 760032Colombia Clínica de la mujer y el niño Universidad Cooperativa de ColombiaPasto 52001Colombia Clínica de Espectro de Acretismo Placentario Fundación Valle del LiliCali 760032Colombia

Congenital diaphragmatic hernia consists of a defect in the embryonic development of the diaphragm that allows the passage of the abdominal viscera into the thoracic cavity,its diagnosis during pregnancy is quite *** present the case of a 31-year-old woman,with 23 weeks of gestation,who consulted for epigastric pain,nausea,and repetitive emetic episodes,without improvement with the medication *** to the intense abdominal pain,a computed tomography of the abdomen and thorax was performed where the 28 mm defect was found at the left diaphragmatic level with protrusion of the gastric fundus to the thoracic *** was taken to surgical management by laparoscopy with abdominal and thoracic approach,with a successful result and without maternal perinatal *** the integrity of the diaphragmatic suture could be feared in relation to the increase in intraabdominal pressure due to uterine growth,the evolution of our patient and previous reports show that postoperative complications are not *** vaginal delivery has even been described in some *** hernias diagnosed during pregnancy are quite *** suggest that the optimal management of them during pregnancy is immediate surgical correction in case of persistent symptoms,more studies are needed to establish firm recommendations on the management of this pathology.

关键词： Hernias diaphragmatic congenital Bochdalek Pregnancy

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Dual role of FGF in proliferation and endoreplication of Drosophila tracheal adult progenitor cells

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Journal of Molecular Cell Biology 2020年第1期12卷 32-41页

作者： Cristina de Miguel josefa Cruz David Martin Xavier Franch-Marro Institute of Evolutionary Biology(Consejo Superior de Investigaciones Cientificas-Universitat Pompeu Fabra) Passeig Maritim de la Barceloneta 3708003 BarcelonaSpain

Adult progenitor cells activation is a key event in the formation of adult *** Drosophilat formation of abdominal adult trachea depends on the specific activation of tracheal adult progenitors(tracheoblasts)at the Tr4 and Tr5 spiracular *** of these tracheoblasts generates a pool of tracheal cells that migrate toward the posterior part of the trachea by the activation of the branchless/fibroblast growth factor(Bnl/FGF)signaling to form the abdominal adult ***,we show that,in addition to migration,Bnl/FGF signaling,mediated by the transcription factor Pointed,is also required for tracheoblast *** tracheoblast activation relies on the expression of the FGF ligand bnl in their nearby ***,we show that,in the absence of the transcription factor Cut(Ct),Bnl/FGF signaling induces endoreplication of tracheoblasts partially by promoting fizzy-related ***,our results suggest a dual role of Bnl/FGF signaling in tracheoblasts,inducing both proliferation and endoreplication,depending on the presence or absence of the transcription factor Ct,respectively.

关键词： FGF trachea adult progenitors cut proliferation endoreplication Pnt Fzr

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Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams

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Journal of Animal Science and Biotechnology 2023年第6期14卷 2395-2410页

作者： Alejandro Jurado‑Campos Pedro Javier Soria‑Meneses Maria Arenas‑Moreira Carlos Alonso‑Moreno Virginia Rodriguez‑Robledo Ana josefa Soler Jose Julian Garde Maria del Rocio Fernandez‑Santos SaBio IREC(CSIC-UCLM-JCCM) Campus UniversitarioS/N02071 AlbaceteSpain. Departamento de Quimica Inorganica Organica Y Bioquimica‑Centro de Innovacion en Quimica Avanzada(ORFEO‑CINQA)Facultad de FarmaciaUniversidad de Castilla-La Mancha02008 AlbaceteSpain. Centro Regional de Investigacion Biomedicas Unidad nanoDrugUniversidad de Castilla-La Mancha02008 AlbaceteSpain. Facultad de Farmacia Universidad de Castilla La Mancha02071 AlbaceteSpain.

Background Artificial insemination(AI)is a routine breeding technology in animal ***,the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in *** this sense,nanotechnology is an interesting tool to improve sperm protection due to the development of nanomaterials for AI,which could be used as delivery *** this work,we explored the feasibility of vitamin E nanoemulsion(NE)for improving sperm quality during *** With the aim of evaluating this proposal,ejaculates of 7 mature rams of Manchega breed were collected by artificial vagina and extended to 60×10^(6)spz/mL in *** containing control and NE(12 mmol/L)with and without exogenous oxidative stress(100μmol/L Fe2+/ascorbate)were stored at 22 and 15℃and motility(CASA),viability(YO-PRO/PI),acrosomal integrity(PNA-FITC/PI),mitochondrial membrane potential(Mitotracker Deep Red 633),lipoperoxidation(C11 BODIPY 581/591),intracellular reactive oxygen species(ROS)production and DNA status(SCSAR)monitored during 96 *** results show that NE could be used to maintain ram spermatozoa during transport at 15 and 22℃for up to 96 h,with no appreciable loss of kinematic and physiological characteristics of freshly collected *** The storage of ram spermatozoa in liquid form for 2-5 d with vitamin E nanoemulsions may lead more flexibility to breeders in AI *** view of the potential and high versatility of these nanodevices,further studies are being carried out to assess the proposed sperm preservation medium on fertility after artificial insemination.

关键词： Breeding technology Nanoemulsions Nanotechnology Sperm oxidative stress Vitamin E

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Impairment of pre-mRNA splicing in liver disease: Mechanisms and consequences

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World Journal of Gastroenterology 2010年第25期16卷 3091-3102页

作者： Carmen Berasain Saioa Gońi josefa Castillo Maria Ujue Latasa Jesús Prieto Matias A Avila Division of Hepatologyand Gene Therapy CIMA University of Navarra Pamplona31008 Spain

Pre-mRNA splicing is an essential step in the process of gene expression in eukaryotes and consists of the removal ofintrons and the linking of exons to generate mature mRNAs. This is a highly regulated mechanism that allows the alternative usage of exons, the retention ofintronic sequences and the generation of exonic sequences of variable length. Most human genes undergo splicing events, and disruptions of this process have been associated with a variety of diseases, including cancer. Hepatocellular carcinoma (HCC) is a molecularly heterogeneous type of tumor that usually develops in a cirrhotic liver. Alterations in pre-mRNA splicing of some genes have been observed in liver cancer, and although still scarce, the available data suggest that splicing defects may have a role in hepatocarcinogenesis. Here we briefly review the general mechanisms that regulatepre-mRNA splicing, and discuss some examples that illustrate how this process is impaired in liver tumorigenesis, and may contribute to HCC development. We believe that a more thorough examination of pre-mRNA splicing is still needed to accurately draw the molecular portrait of liver cancer. This will surely contribute to a better understanding of the disease and to the development of new effective therapies.

关键词： Cell signalling Hepatocarcinogenesis PremRNA splicing Splicing factors Targeted anticancer therapy

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Effect of Coordinate Bond in Molecular Recognition of Enrofloxacin with Imprinted Polymers

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复旦学报（自然科学版） 2007年第5期46卷 803-804页

作者： Recilas-mota J Jesús Gracia-mora Jesús Bernad-bernad josefa Department of Inorganic Chemistry National Autonomous of Mexico University México City Department of Inorganic Chemistry National Autonomous of Mexico University México City04510.México Department of Pharmacy National Autonomous of México UniversityMéxico City04510México

1 Results Molecular imprinting is a technique for the preparation of functional materials with molecular recognition *** imprinted polymers (MIPs) have become an increasingly active field of study for the construction of new material capable of molecular *** general,MIPs are synthesized by polymerization of cross-linking complexes of template molecules and functional *** removing the template molecules from de polymers,binding sites are formed by functional monomer...

关键词： imprinted polymers coordinated bond molecular recognition

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Low-cost experimental application of real-time kinematic positioning for increasing the benefits in cereal crops

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International Journal of Agricultural and Biological Engineering 2021年第3期14卷 194-199页

作者： Abdelhamid Tayebi josefa Gómez Marián Fernández Francisco Sáez de Adana Oscar Gutiérrez Computer Science Department University of AlcaláAlcaláde Henares 28801Spain

Traditional agriculture is facing several challenges worldwide such as increased population growth,rapid forestry and urbanization,resource scarcity,climate change,environmental pollution,competition among different ***,farmers need to improve productivity in order to maintain the output *** study attempted to evaluate the benefits of using Real-Time Kinematic(RTK)positioning in precision agriculture through a series of real measurements carried out when farming *** farming management actions involved in the cereal crop process(raise fallow,plow,sow,fertilize,mow,and harvest)have been done using an automatic guidance system that has reduced costs.A reduction of 20%has been quantified in the fuel,the amount of fertilizer,the labor costs and the hours of ***,the environmental impact has been also *** inexpensive system consisting of a reference base station near the field and a mobile unit mounted on the test vehicle has been installed in order to increase the benefits in cereal *** Navigation Satellite System(GNSS)systems including Global Positioning System(GPS),GLONASS,Galileo and Beidou have been used in the *** research serves as a practical guide to implementing a low-cost guidance system to achieve best management practice.

关键词： real-time kinematic low-cost positioning benefits guidance system cereal

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Current status and potential of morphometric sperm analysis

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Asian Journal of Andrology 2016年第6期18卷 863-870页

作者： Alejandro Maroto-Morales Olga Garcia-Alvarez Manuel Ramon Felipe Martinez-Pastor M Rocio Fernandez-Santos A josefa Soler Jose Julian Garde SaBio IREC (CSIC - UCLM -JCCM) Albacete Spain Biomedical Center Medical Faculty in Pilsen Charles University in Prague Pilsen Czech Republic Regional Center of Animal Selection and Reproduction (CERSYRA) JCCM Valdepenas Spain 4Institute for Animal Health and Cattle Development University of Leon LeOn Spain Department of Molecular Biology University of Leon Leon Spain Faculty of Pharmacy (UCLM) Albacete Spain

The spermatozoon is the most diverse cell type known and this diversity is considered to reflect differences in sperm function. How the diversity in sperm morphology arose during speciation and what role the different specializations play in sperm function, however, remain incompletely characterized. This work reviews the hypotheses proposed to explain sperm morphological evolution, with a focus on some aspects of sperm morphometric evaluation; the ability of morphometrics to predict sperm cryoresistance and male fertility is also discussed. For this, the evaluation of patterns of change of sperm head morphometry throughout a process, instead of the study of the morphometric characteristics of the sperm head at different stages, allows a better identification of the males with different sperm cryoconservation ability. These new approaches, together with more studies employing a greater number of individuals, are needed to obtain novel results concerning the role of sperm morphometry on sperm function. Future studies should aim at understanding the causes of sperm design diversity and the mechanisms that generate them, giving increased attention to other sperm structures besides the sperm head. The implementation of scientific and technological advances could benefit the simultaneous examination of sperm phenotype and sperm function, demonstrating that sperm morphometry could be a useful tool for sperm assessment.

关键词： computer-assisted sperm morphometric analysis mammals sperm function sperm morphometry

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