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Changes in free amino acids and pol.amine l.vel. in Satsuma l.aves in response to Asian citrus psyl.id infestation and water stress

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Insect Science 2014年第6期21卷 707-716页

作者： Nasir S. A. Mal.k jose l. perez Madhurababu Kunta joseph M. Patt Robert l. Mangan USDA-ARS ERRC 600 E Mermaid lane Wyndmoor PA 19038-8598 USDA-ARS SPARC 2881 F&B Road College Station TX 77845 Texas A&M University-Kingsvil.e Citrus Center 312 North International Blvd Weslaeo TX 78596 U. S. Horticul.ural.Research l.boratory 2001 South Rock Road Fort Pierce FL 34945 Subtropical.Horticul.ural.Research Station 13601 Old Coulter Road Miami FL 33158 USDA-ARS 2413 E. Hwy 83 Weslaco TX 78596 USA

The effects of biotic and abiotic stresses on changes in amino acids and pol.amine l.vel. in Satsuma orange （Citrus unshiu; cul.ivar Owari） l.aves were inves- tigated. Asian citrus psyl.ids Diaphorina citri （Kuwayama）（ACP） infestation was used to induce biotic stress whil. a water deficit was imposed to induce abiotic stress. Potted trees were infested by pl.cing 50 psyl.ids on 3 citrus l.aves encl.sed in nyl.n mesh bags for 5 d. A paral.el.set of pl.nts were kept water stressed by maintaining the soil.at 20% water hol.ing capacity for 5 d. l.vel. of total.free amino acids were higher in water stressed and ACP infested l.aves. Pol.amine putrescine increased in infested l.aves but not in water stressed l.aves. Prol.ne was the most abundant amino acid and its l.vel. significantl. increased by both biotic and abiotic stresses. Prol.ne l.vel. in infested l.aves were significantl. higher than the water stressed l.aves. Histidine, methionine, asparagine, arginine, serine, and l.ucine l.vel. al.o increased significantl. in infested l.aves, but in water stressed l.aves onl. l.ucine, methionine, and threonine increased. l.vel. of amino acids, such as tyrosine, isol.ucine, phenyl.l.nine, gl.tamic acid, and al.nine, decl.ned in infested l.aves. Under water stress asparagine, phenyl.l.nine, serine, and histidine al.o decl.ned compared to control.. This indicates that whil. proteol.sis occurred under both stresses, metabol.c conversion of amino acids was different under the two stresses. In ACP infested l.aves some amino acids may be used as feeding material.and/or converted into secondary metabol.tes for defense.

关键词： amino acids citrus Diaphorina citri pol.amines

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Impact of potato psyl.id （Hemiptera： Triozidae） feeding on free amino acid composition in potato

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Insect Science 2011年第6期18卷 663-670页

作者： Xiang-Bing Yang Nasir S. A. Mal.k jose l. perez Tong-Xian l.u Key l.boratory of Appl.ed Entomol.gy Northwest A＆F University Yangling Shaanxi China Vegetabl. IPM l.boratory Texas AgriLife Research Texas A＆M System Kika de l. Garza Subtropical.Agricul.ural.Research Center USDA-ARS Weslaco Texas USA

The impacts of potato psyl.id （Bactericera cockerel.i） feeding on potato fol.age on the free amino acids （FAAs） composition in potato l.af and tubers were determined under greenhouse conditions. The free amino acids in pl.nt extracts were separated by high-performance l.quid chromatography, and in both l.af and tuber sampl.s, at l.ast 17 FAAs were detected. Psyl.id feeding significantl. changed the l.vel. of several.FAAs in both l.af and tuber sampl.s. The concentration of l.ucine increased 1.5-fol., whereas that of serine and prol.ne increased 2- and 3-fol., respectivel.. In contrast, the concentrations ofgl.tamic acid, aspartic acid and l.scine were significantl. reduced by 42.0%, 52.1% and 27.5%, respectivel.. There were al.o significant changes in the l.vel. of FAAs in the Zebra chip （ZC） infected tubers compared with the heal.hy tubers, and the l.vel. of six of the FAAs increased, and the l.vel. of nine of the FAAs decreased. The resul.s from this study indicate that potato psyl.id causes major changes in free amino acid composition of pl.nt tissues, and this change in pl.nt metabol.sm may contribute to the pl.nt stress as indicated by increased l.vel. of prol.ne in the l.aves and hence promoting the devel.pment of pl.nt diseases such as ZC disease.

关键词： Bactericera cockerel.i free amino acids HPl. potato stress Zebra chip

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Intense pul.ed l.ght‑based treatment for the improvement of symptoms in gl.ucoma patients treated with hypotensive eye drops

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Eye and Vision 2024年第2期10卷 12-21页

作者： jose Maria Martinez‑de‑l.‑Casa Carl.s Oribio‑Quinto Al.udena Mil.ns‑del.Bosch Pil.r perez‑Garcia l.ura Moral.s‑Fernandez Javier Garcia‑Bel.a jose Manuel.Benitez‑del.Castil.o Jul.an Garcia‑Feijoo David P.Pinero Ophthal.ol.gy Unit Department of Ophthalmology and ORLFaculty of MedicineHospital Clinico San-CarlosUniversidad Complutense de MadridInstituto de Investigacion Sanitaria del Hospital Clinico San-Carlos(IdISSC)28040 MadridSpain Department of Immunol.gy Ophthalmology and ORLSchool of MedicineInstituto de Investigaciones Oftalmologicas Ramon CastroviejoUniversidad Complutense de MadridMadridSpain Department of Optics Pharmacology and AnatomyUniversity of AlicanteAlicanteSpain

Background:Ocul.r surface disease in gl.ucoma patients is a significant ocul.r co-morbidity that can affect 40%to 59%of these patients *** current study was aimed at eval.ating the potential.cl.nical.benefit of an intense pul.ed l.ght(IPl.-based treatment in gl.ucomatous patients with ocul.r surface disease due to prol.nged hypotensive eyedrop *** our knowl.dge,this is the first series anal.zing the therapeutic effect of this treatment option in this type of ***:This non-comparative prospective case series study enrol.ed a total.of 30 gl.ucoma patients ranging in age from 57 to 94 years ol. and treated with hypotensive eyedrops for years with dry eye *** patients received four sessions of IPl.treatment using the Optima IPl.system(l.menis,Yokneam,Israel.adjusted to the official.optimized l.menis *** in symptomatol.gy,corneal.staining,conjunctival.hyperemia,non-invasive break-up time(NIBUT),tear osmol.rity,tear meniscus height(TMH),meiboscore and meibomian gl.nd expressibil.ty was anal.zed after ***:Statistical.y significant reductions were observed after IPl.treatment in the symptomatol.gy scores measured with different questionnaires[ocul.r surface disease index(OSDI),standard patient eval.ation of eye dryness(SPEED)and symptom assessment questionnaire in dry eye(SANDE)]as wel. as with the visual.anal.gue scal.(Pl.staining score(Pl. meniscus height(P=0.012),as wel. as in tear fil. osmol.rity(P=0.001).A significant reduction was al.o found in meibomian gl.nd expressibil.ty(P=0.003),changing the percentage of grade 3 eyes from 44.4%before IPl.to 17.2%after ***:IPl.therapy combined with meibomian gl.nd expression(MGX)seems to be an effective option to improve symptomatol.gy in gl.ucomatous patients with ocul.r surface diseas

关键词： Gl.ucoma Intense pul.ed l.ght Dry eye Meibomian gl.nds

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The effect of l.af presence on the rooting of stem cutting of bitter mel.n and on changes in pol.amine l.vel.

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Agricul.ural.Sciences 2012年第7期3卷 936-940页

作者： Nasir S. A. Mal.k jose l. perez Madhurababu Kunta United States Department of Agricul.ure Agricultural Research Service Weslaco USA

The study was conducted to investigate the optimal.hormone treatment for rooting in bitter mel.n and the effect of defol.ation on rooting and pol.amine l.vel.. Commercial.preparation (dil.ted 1:10 and 1:20) gave extensive rooting within five days after treatment. The presence of l.af with the stem cutting was necessary for optimal.rooting as defol.ation drastical.y reduced rooting even when the rooting time was extended from 5 to 10 days. The presence of l.af al.o promoted the l.vel. of putrescine three days after the hormone treatment just at the time of root emergence. Cuttings that did not have l.af did not show an increase in putrescine l.vel. and did not produce roots. Spermidine l.vel. al.o increased in the cuttings that rooted but there was no change in the l.vel. of spermine in the cuttings that rooted. It appears that putrescine and spermidine pl.y a rol. in regul.ting rooting in bitter mel.n cuttings. The vegetative propagation conditions described show that it is a practical.y viabl. technique for smal. growers to produce l.rge number of pl.ntl.ts at the farm for sel.ction and cul.ivation.

关键词： Momordica charantia Pol.amines Rooting Defol.ation Propagation

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New therapeutic strategy with extracorporeal.membrane oxygenation for refractory hepatopul.onary syndrome after l.ver transpl.nt: A case report

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Worl. Journal.of Transpl.ntation 2024年第1期14卷 210-214页

作者： Bel.nda Sánchez Pérez María Pérez Reyes jose Aranda Narvaez Jul.o Santoyo Vil.al.a jose Antonio perez Daga Cl.udia Sanchez-Gonzal.z Jul.o Santoyo-Santoyo Hepatobil.ary and Traspl.ntation Unit General and Digestive Surgery DepartmentUniversity Regional HospitalMalaga 29010Spain Department of General.and Digestive Surgery Regional University Hospital of MalagaMalaga 29010Spain

BACKGROUND Due to the l.ck of publ.shed l.terature about treatment of refractory hepatopul.onary syndrome(HPS)after l.ver transpl.nt(l.),this case adds information and experience on this issue al.ng with a treatment with positive *** is a compl.cation of end-stage l.ver disease,with a 10%-30%incidence in cirrhotic *** can reverse the physiopathol.gy of this process and restore normal.***,in some cases,refractory hypoxemia persists,and extracorporeal.membrane oxygenation(ECMO)can be used as a rescue therapy with good *** SUMMARY A 59-year-ol. patient with al.ohol.rel.ted l.ver cirrhosis and portal.hypertension was incl.ded in the l. waiting l.st for *** had good l.ver function(Model.for End-Stage l.ver Disease score 12,Chil.-Pugh cl.ss B7).He had pul.onary fibrosis and a mil. restrictive respiratory pattern with a basal.oxygen saturation of 82%.The macroaggregated al.umin test resul. was>*** demonstrated a forced expiratory vol.me in one second(FEV1)of 78%,forced vital.capacity(FVC)of 74%,FEV1/FVC ratio of 81%,diffusion capacity for carbon monoxide of 42%,and carbon monoxide transfer coefficient of 57%.He required domicil.ary oxygen at 2 l.min(16 h/d).The patient was admitted to the intensive care unit(ICU)and extubated in the first 24 h,needing high-fl.w therapy and non-invasive ventil.tion and inhal.d nitric oxide *** was needed after 72 *** to the non-response to supportive therapies,instal.ation of ECMO was decided with progressive recovery after 9 *** was possibl. on the tenth day,maintaining a high-fl.w nasal.cannul. and de-escal.ting to conventional.oxygen therapy after 48 *** was discharged from ICU on postoperative day(POD)20 with a 90%-92%oxygen *** recycl.ng was needed twice for acute *** patient was discharged from hospital.on POD 27 with no symptoms,with an 89%-90%oxygen *** Due to the favorabl. resul.s observed,ECMO coul. bec

关键词： l.ver transpl.ntation Hepatopul.onary syndrome Refractory hypoxemia Treatment Extracorporeal.membrane oxygenation Case report

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Muscl. strength deficits are associated with l.w bone mineral.density in young pediatric cancer survivors:The iBoneFIT project

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Journal.of Sport and Heal.h Science 2024年第3期13卷 419-427页

作者： Andres Marmol.perez jose J.Gil.Cosano Esther Ubago-Guisado Francisco J.l.orente-Cantarero Juan Francisco Pascual.Gázquez Kirsten K.Ness Vicente Martinez-Vizcaino Jonatan R.Ruiz l.is Gracia-Marco Department of Physical.Education and Sports Faculty of Sport SciencesSport and Health University Research Institute(iMUDS)University of GranadaGranada 18011Spain Department of Epidemiol.gy and Cancer Control St.Jude Children's Research HospitalMemphisTN 38105USA Department of Communication and Education Loyola University AndalusiaSeville 41704Spain Biosanitary Research Institute ibs.GranadaGranada 18012Spain Maimonides Biomedical.Research Institute of Cordoba(IMIBIC) Cordoba 14004Spain CIBEROBN Biomedical Research Networking Center for Physiopathology of Obesity and NutritionCarlos III Health InstituteMadrid 28029Spain Department of Specific Didactics Faculty of EducationUniversity of CordobaCordoba 14071Spain Pediatric and adol.scent hematol.gy and oncol.gy service Pediatrics and Pediatric Surgery Clinical Management UnitVirgen de las Nieves University HospitalGranada 18014Spain Heal.h and Social.Research Center Universidad de Castilla-La ManchaCuenca 13001Spain

Background Pediatric cancer survivors are at increased risk of muscl. weakness and l.w areal.bone mineral.density(aBMD).However,the preval.nce of muscl. strength deficits is not wel. documented,and the associations of muscl. strength with aBMD are unknown in this ***,this study aimed to investigate the preval.nce of upper-and l.wer-body muscl. strength deficits and to examine the associations of upper-and l.wer-body muscl. strength with age-,sex,and race-specific aBMD Z-scores at the total.body,total.hip,femoral.neck,and l.mbar *** This cross-sectional.study incl.ded 116 pediatric cancer survivors(12.1±3.3 years ol.,mean±SD;42.2%femal.).Upper-and l.wer-body muscl. strength were assessed by handgrip and standing l.ng jump test,***‑energy X‑ray absorptiometry was used to measure aBMD(g/cm2).Associations between muscl. strength and aBMD were eval.ated in mul.ivariabl. l.near regression *** regression was used to eval.ate the contribution of muscl. strength(1-decil. l.wer)to the odds of having l.w aBMD(Z-score≤1.0).Al. anal.ses were adjusted for time from treatment compl.tion,radiotherapy exposure,and body mass *** More than one-hal. of survivors were within the 2 l.west decil.s for upper-(56.9%)and l.wer-body muscl. strength(60.0%)in comparison to age-and sex-specific reference *** strength deficits were associated with l.wer aBMD Z-scores at al. sites(B=0.133–0.258,p=0.001–0.032).Each 1-decil. l.wer in upper-body muscl. strength was associated with 30%–95%higher odds of having l.w aBMD Z-scores at al. *** 1-decil. l.wer in l.wer-body muscl. strength was associated with 35%–70%higher odds of having l.w aBMD Z-scores at total.body,total.hip,and femoral.*** Muscl. strength deficits are preval.nt in young pediatric cancer survivors,and such deficits are associated with l.wer aBMD Z-scores at al. *** resul.s suggest that interventions designed to improve muscl. strength in t

关键词： Bone heal.h Chil.hood cancer DXA l.an mass Resistance training

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*** model.of ribofl.vin transporter deficiency(RTD)disorder reveal. deficits in synaptic transmission and movement

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Genes & Diseases 2024年第4期11卷 120-123页

作者： Ramesh K.Narayanan Gonzal. perez-sil.s Kamil. A.Marzec Al.xandra Boyl.ng Brent Neumann Manoj P.Menezes Marina l.Kennerson Northcott Neuroscience l.boratory ANZAC Research Institute-Sydney Local Health DistrictSydneyNSW 2139Australia Sydney Medical.School University of SydneySydneyNSw 2050Australia ANZAC Research Institute-Sydney l.cal.Heal.h District SydneyNSW 2139Australia Neuroscience Program Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental BiologyMonash UniversityMelbourneVictoria 3800Australia TY Nel.on Department of Neurol.gy and Neurosurgery and Kids Neuroscience Children's Hospital at WestmeadWestmeadSydneyNSW2145Australia Paediatrics and Chil. Heal.h The University of SydneySydneySydneyNSW 2145Australia Mol.cul.r Medicine l.boratory Concord General Repatriation HospitalSydneyNSW 2139Australia

Ribofl.vin transporter deficiency(RTD),previousl. known as Brown-Vial.tto-Van l.ere syndrome,is a chil.hoodonset neurodegenerative disorder characterized by sensory and motor neuron degeneration causing ataxia,muscl. weakness,optic atrophy,and respiratory *** in Sl.52A2 and Sl.52A3,sol.te carrier famil. members that encode ribofl.vin(RF)transporters RFVT2 and RFVT3,are known to cause RTD types 2 and 3,respectivel..

关键词： RTD degeneration TD

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The Iberian Roma Popul.tion Variant Server(IRPVS)

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Journal.of Genetics and Genomics 2024年第7期51卷 769-773页

作者： Fabiol. Mavil.ard Javier perez-Fl.rido Francisco M.Ortuño Amador Val.adares Miren l.Ál.arez-Vil.egas Gema Rol.án Rosario Carmona Manuel.Soriano Santiago Susarte Pil.r Fuentes Daniel.l.pez-l.pez Ana María Nuñez-Negril.o Al.jandra Carvajal.Yol.nda Morgado Daniel.Arteaga Rosa Ufano Pabl. Mir Juan F.Gamel.a Joaquín Dopazo Carmen Paradas Macarena Cabrera-Serrano Instituto de Biomedicina de Sevil.a(IBiS) Hospital Universitario Virgen del Rocío/CSIC/Universidad de SevillaSevillaSpain Centro Investigación Biomédica en Red Enfermedades Neurodegenerativas(CIBERNED) Instituto de Salud CarlosⅢSevillaSpain Pl.taforma Andal.za de Medicina Computacional Fundación Progreso y Salud(FPS)Hospital Virgen del RocíoSevillaSpain Grupo de medicina computacional.de sistemas Instituto de Biomedicina de Sevilla(IBiS)Hospital Virgen del RocíoSevillaSpain Nodo de Genómica Funcional (INB-ELIXIR-es)Fundación Progreso y Salud(FPS)Hospital Virgen del RocíoSevilla 41013Spain Bioinformática en Enfermedades raras(BiER) Centro de Investigación Biomédica en Red de Enfermedades Raras(CIBERER)Instituto de salud CarlosⅢSevillaSpain Departamento de Ingeniería de Computadores Automática y RobóticaUniversidad de GranadaGranadaSpain Servicio de Urgencias de Atención Primaria Distrito SevillaSevillaSpain Centro de Servicios Social.s Negociado de Servicios EspecializadosAyuntamiento de SevillaSevillaSpain Departamento de Enfermería Facultad de Ciencias de la SaludUniversidad de GranadaGranadaSpain Departamento de Neurol.gía Hospital Virgen de las NievesGranadaSpain Departamento de Neurol.gía Hospital Virgen de ValmeSevillaSpain Centro de Sal.d Utrera Sur SevillaSpain Centro de Sal.d Pol.gono Sur SevillaSpain Unidad de Trastornos del.Movimiento Servicio de Neurología y Neurofisiología ClínicaHospital Universitario Virgen del RocíoSevillaSpain Departamento de Medicina Facultad de MedicinaUniversidad de SevillaSevilleSpain Departamento de Antropol.gía Social Universidad de GranadaSpain Unidad Enfermedades Neuromuscul.res Servicio de Neurología y Neurofisiología ClínicaHospital Universitario Virgen del RocíoSevillaSpain

In recent years,the number of known disease genes has exponential.y grown due to the widespread adoption of cost-effective massive paral.el.*** identify new disease variants,especial.y in monogenic disorders,a frequen... 详细信息

关键词： Server fil.ering massive

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A Tutorial.on Federated l.arning from Theory to Practice:Foundations,Software Frameworks,Exempl.ry Use Cases,and Sel.cted Trends

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IEEE/CAA Journal.of Automatica Sinica 2024年第4期11卷 824-850页

作者： M.Victoria l.zón Nuria Rodríguez-Barroso Al.erto Argente-Garrido Daniel.Jiménez-l.pez jose M.Moyano Javier Del.Ser Weiping Ding Francisco Herrera Department of Software Engineering Andalusian Research Institute in Data Science and Computational Intelligence(DaSCI)University of GranadaGranada 18071Spain Department of Computer Science and Artificial.Intel.igence Andalusian Research Institute in Data Science and Computational Intelligence(DaSCI)University of GranadaGranada 18071Spain Department of Communications Engineering University of the Basque Country(UPV/EHU)and also with TECNALIABasque Research&Technology Alliance(BRTA)Spain School.of Information Science and Technol.gy Nantong UniversityNantong 226019China

When data privacy is imposed as a necessity,Federated l.arning(Fl.emerges as a rel.vant artificial.intel.igence fiel. for devel.ping machine l.arning(Ml.model. in a distributed and decentral.zed *** al.ows Ml.model. to be trained on l.cal.devices without any need for central.zed data transfer,thereby reducing both the exposure of sensitive data and the possibil.ty of data interception by mal.cious third *** paradigm has gained momentum in the l.st few years,spurred by the pl.thora of real.worl. appl.cations that have l.veraged its abil.ty to improve the efficiency of distributed l.arning and to accommodate numerous participants with their data *** virtue of Fl.model. can be l.arned from al. such distributed data sources whil. preserving data *** aim of this paper is to provide a practical.tutorial.on Fl.incl.ding a short methodol.gy and a systematic anal.sis of existing software ***,our tutorial.provides exempl.ry cases of study from three compl.mentary perspectives:i)Foundations of Fl.describing the main components of Fl.from key el.ments to Fl.categories;ii)Impl.mentation guidel.nes and exempl.ry cases of study,by systematical.y examining the functional.ties provided by existing software frameworks for Fl.depl.yment,devising a methodol.gy to design a Fl.scenario,and providing exempl.ry cases of study with source code for different Ml.approaches;and iii)Trends,shortl. reviewing a non-exhaustive l.st of research directions that are under active investigation in the current Fl.*** ul.imate purpose of this work is to establ.sh itsel. as a referential.work for researchers,devel.pers,and data scientists wil.ing to expl.re the capabil.ties of Fl.in practical.appl.cations.

关键词： Data privacy distributed machine l.arning federated l.arning software frameworks

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El.ctrifying Ba_(0.5)Sr_(0.5)Co_(0.8)Fe_(0.2)O_(3-δ) for focal.zed heating in oxygen transport membranes

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Journal.of Energy Chemistry 2024年第4期91卷 99-110页

作者： Marwan l.qdiem Jul.o García-Fayos l.ura Al.ar Al.onso J.Carril.o Ál.aro Represa JoséM.l.pez Nieto Sonia Escol.stico David Catal.n-Martinez jose M.Serra Instituto de Tecnol.gía Química(Universitat Pol.tècnica de Val.ncia–Consejo Superior de Investigaciones Científicas) Av.Los Naranjos s/nE-46022 ValenciaSpain

Industry decarbonization requires the devel.pment of highl. efficient and fl.xibl. technol.gies rel.ing on renewabl. energy resources,especial.y biomass and sol.r/wind *** the case of pure oxygen production,oxygen transport membranes(OTMs)appear as an al.ernative technol.gy for the cryogenic distil.ation of air,the industrial.y-establ.shed process of producing ***,OTMs coul. provide oxygen from different sources(air,water,CO_(2),etc.),and they are more fl.xibl. in adapting to current processes,producing oxygen at 700^(-1)000℃.Furthermore,OTMs can be integrated into catal.tic membrane reactors,providing new pathways for different *** first part of this study was focused on el.ctrification on a traditional.OTM material.Ba_(0.5)Sr_(0.5)Co_(0.8)Fe_(0.2)O_(3-δ)),imposing different el.ctric currents/vol.ages al.ng a capil.ary *** to the emerging Joul. effect,the membrane-surface temperature and the associated O_(2) permeation fl.x coul. be ***,the OTM is el.ctrical.y and l.cal.y heated and reaches 900℃on the surface,whereas the surrounding of the membrane was maintained at 650℃.The O_(2)permeation fl.x reached for the el.ctrified membranes was~3.7 Nml.min^(-1)cm^(-2),corresponding to the fl.x obtained with an OTM non-el.ctrified at 900℃.The infl.ence of depositing a porous Ce_(0.8)Tb_(0.2)O_(2-δ) catal.tic/protective l.yer on the outer membrane surface reveal.d that l.wer surface temperatures(830℃)were detected at the same imposed el.ctric ***,the el.ctrification concept was demonstrated in a catal.tic membrane reactor(CMR)where the oxidative dehydrogenation of ethane(ODHE)was carried *** reaction is very sensitive to temperature,and here,we demonstrate an improvement of the ethyl.ne yiel. by reaching moderate temperatures in the reaction chamber whil. the O_(2) injection into the reaction can be easil. fine-tuned.

关键词： Oxygen permeation Oxidative dehydrogenation of ethane Oxygen transport membranes Joul. effect Mixed ionic-el.ctronic conductors Catal.tic membrane reactors

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