检索结果-南通市图书馆

Study and ICH validation of a reverse-phase liquid chromatographic method for the quantification of the intact monoclonal antibody cetuximab

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2016年第2期6卷 117-124页

作者： Antonio Martinez-Ortega Agustin Herrera Antonio Salmeron-Garcia jose Cabeza luis Cuadros-Rodriguez Natalia Navas Department of Analytical Chemistry University of Granada Faculty of Science Campus Fuentenueva s/n E-18071 Granada Spain UGC intercentro Interniveles Farmacia Granada San Cecilio Hospital Biomedical Research Institute ibs. GRANADA. Hospitales Universitarios de Granada University of Granada E-18012 Granada Spain Department of Analytical Chemistry Science Faculty Biomedical Research Institute ibis. GRANADA University of Granada Campus Fuentenuevo s/n E-18071 Granada Spain

Cetuximab （CTX） is a potent chimeric mouse/human monoclonal antibody （mAb） approved worldwide for treatment of metastatic colorectal cancer. Among the various biological and physical analyses per- formed for full study on this biopharmaceutic, the determination of the concentration preparations throughout manufacturing and subsequent handling in hospital is particularly relevant. In the present work, the study and validation of a method for quantifying intact CTX by reverse-phase high-perfor- mance liquid chromatography with diode array detection （（RP）HPLC/DAD） is presented. With that end, we checked the performance of a chromatographic method for quantifying CTX and conducted a study to validate the method as stability-indicating in accordance with the International Conference on Harmo- nization guidelines （ICH） for biotechnological drugs; therefore, we evaluated linearity, accuracy, preci- sion, detection and quantification limits, robustness and system suitability. The specificity of the method and the robustness of the mAb formulation against external stress factors were estimated by compre- hensive chromatographic analysis by subjecting CTX to several informative stress conditions. As de- monstrated, the method is rapid, accurate, and reproducible for CTX quantification. It was also suc- cessfully used to quantify CTX in a long-term stability study performed under hospital conditions.

关键词： Reverse-phase high performance liquidchromatography Diode detector Stress study Biopharmaceuticals Cetuximab

Development and test verification of air temperature model for Chinese solar and Spainish Almeria-type greenhouses

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

International Journal of Agricultural and Biological Engineering 2017年第4期10卷 66-76页

作者： Jorge Antonio Sanchez-Molina Li Ming Francisco Rodriguez jose luis Guzman Wang Hui Yang Xinting The Automatic Control Robotics and Mechatronics Research GroupDepartment of InformaticsceiA3CIESOLUniversity of AlmeriaAlmeria 04120Spain Beijing Research Center for Information Technology in Agriculture/National Engineering Research Center for Information Technology in Agriculture/National Engineering Laboratory for Agri-product Quality Traceability/Key Laboratory for Information Technologies in Agriculture Ministry of AgricultureBeijing 100097China

Growth can be defined as an increment in biomass or an increment in weight or height of the organs of the plant influenced by physiological *** of these processes have their limits genetically determined,but climate and irrigation play an important *** of its importance,microclimate has been extensively studied in the modeling as a surrounding condition which is imposed by the exterior *** main objective of this work was to develop a temperature model based on the energy balance dynamics at two different greenhouse locations-South-eastern Spain and Northern China,and the traditional structures of Chinese solar greenhouse and Almería-type multi-span greenhouse were taken into *** final model was developed by combining the external conditions,the actuator influence and the crop growth,where the temperature is influenced by soil,crop,cover,actuators,back wall and greenhouse *** model took into account the energy lost by convective and conductive fluxes,as well as the energy supplied by solar radiation and heating *** soil and the back wall are the main media for energy *** temperature dynamic was determined by a physical model,which considered the energy balance from a holistic point of view-as a sub-model for a customizable interface among the external climate,the plant and the greenhouse *** influences of different subsystems included in the temperature model were analyzed and *** results showed a high R^(2)value of 0.94 for Beijing and 0.95 for Almeria,and the average error was low,of which the MAE and RMSE were 0.71 and 1.365 for Almeria and 0.62 and 1.102 for Beijing,***,the model can be considered as a powerful tool for control design purposes in microclimate systems.

关键词： air temperature model Chinese solar greenhouse Spanish Almería-type greenhouse energy balance dynamics microclimate

Unsteady shock front waviness in shock-buffet of transonic aircraft

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Aerodynamics 2020年第1期2卷 182-198页

作者： Razvan Marian Apetrei Vlad Ciobaca jose luis Curiel-Sosa Ning Qin Department of Mechanical Engineering The University of SheffieldSheffieldUK DLR Institute of Aerodynamics and Flow TechnologyBraunschweigGermany

The unsteady transonic aerodynamics of a wing-body configuration is investigated by solving the Unsteady Reynolds-averaged Navier-Stokes equations closed with the full Reynolds Stress *** work presents the prediction of flow field characteristics during deep shock-buffet penetration of a transport aircraftrepresentative *** number of 0.85,and Reynolds number of 5 million based on the mean chord,are selected to reproduce experimental test conditions that serve as validating *** results obtained give information about both surface and flow field shock-buffet *** unsteady shock front is observed on the suction side of the wing which gives birth to the so-called buffet *** field characteristics are dominated by the presence of lambda-shaped shocks and fully separated boundary layer over a significant part of the wing.

关键词： Aerodynamics CFD Shock-buffet

Relation between Spirals in Pre-Hispanic Design with Mathematics Spirals

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Mathematics and System Science 2015年第10期5卷 429-434页

作者： jose luis Mamani Graduated from Faculty of Engineering National University of Jujuy-Argentina.

The spiral has been studied for the first time in the book “About the spirals”, by Archimedes （Greece, 287-212 BC）. In America, the design of spirals seems to have been used previous to Archimedes＇ time, although in the Northwest of Argentina the appearance of this figure in ceramics can be dated between the years 800 and 1,400 （AD）. The spiral is found in cave paintings, in pumpkin carvings and in pottery, particularly those painted inside of plates （pucos in Quechua language）.There can be seen simple linear spirals, double spirals and spirals filled with squares, spirals in two, facing each other symmetrically, as well as spirals joined to bodies of animals. The number of spires detected in the largest spiral is 12, a number of evidently sacred characteristics. When there are several spirals in an earthenware vessel, it can be seen that all of them spin to the same side whether it is clockwise or not. This could have been caused by the predominance of the use of either one hand or the other by the ancient artist. This paper is produced with computer methods used to design spirals in the computer, either Archimedes or the logarithmic ones. Finally, the book： “Man and his symbols” of the psychiatrist Carl Gustav Jung （1875-1961） help to understand this congruencies. In this paper, Mathematics is complemented with the Archeology of art and Psychology.

关键词： Spirals complementarity art conscience unconscious.

Cantharellus(Cantharellales,Basidiomycota)revisited in Europe through a multigene phylogeny

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fungal Diversity 2017年第2期83卷 263-292页

作者： Ibai Olariaga Gabriel Moreno jose luis Manjon Isabel Salcedo Valerie Hofstetter Diego Rodrıguez Bart Buyck Department of Life Sciences(Botany) University of Alcala28805 Alcalade HenaresMadridSpain Department of Plant Biology and Ecology(Botany) University of the Basque Country(UPV/EHU)Apdo 64448080 BilbaoSpain Department of Plant Protection Agroscope ChanginsWadenswil Research StationACWRte de Duiller1260 NyonSwitzerland Kherdzmari St.Microdistrict 11.Block 58 Flat 69TemkaTbilisiGeorgia Departement Systematique et Evolution CP 39ISYEBUMR 7205 CNRS MNHN UPMC EPHEMuseum National d’Histoire Naturelle12 Rue Buffon75005 ParisFrance

Resolving species delimitation issues of European Cantharellus is crucial to correctly name chanterelles around the *** names referring to Cantharellus *** been described in Europe,some of which are used in other *** on combined analyses of ITS2,LSU,RPB2 and TEF-1,merely eight species are here recognized in Europe applying the genealogical concordance phylogenetic species recognition criteria,one of which,***,is described as ***logical characters used in species delimitation are mapped and their variability *** colour of the hymenophore in young specimens is found to be a rather constant morphological character of taxonomic *** species of Cantharellus are morphologically distinguished by unique combinations of characters,such as the presence of a pink pileal coating,pileus and hymenophore colour when young,and in some cases,the mean spore length and *** type specimens from Europe are *** on revised species concepts sixteen novel taxonomic synonyms are here proposed for European chanterelles:***(=***,***,***),***(=*** ***,*** ***,***),***(=*** ***,***),***(=*** ***),***(=***),***(=*** ***,*** ***,***),and ***sianus(=***,***,***nesianus ***).The type of Cantharellus,***,is ***,colour illustrations and a key to all European species are provided.

关键词： Cantharelloid clade Cantharellaceae Epitype Edible fungi Genealogical concordance Species delimitation

Inverted Meckel’s diverticulum diagnosed using capsule endoscopy: A case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2021年第36期27卷 6154-6160页

作者： Ismael El Hajra Martínez Marta Calvo Joséluis Martínez-Porras Lucia Gomez-Pimpollo Garcia jose L Rodriguez Carmen Leon Joséluis Calleja Panero Department of Gastroenterology Hospital Universitario Puerta de HierroMadrid 28222Spain Department of Radiology Hospital Universitario Puerta de HierroMadrid 28222Spain Department of Diagnostic Pathology Hospital Universitario Puerta de HierroMadrid 28222Spain Department of Surgery Hospital Universitario Puerta de HierroMadrid 28222Spain

BACKGROUND Meckel’s diverticulum is a common asymptomatic congenital gastrointestinal ***,its presentation as an inverted Meckel's diverticulum is a rare complication,of which few cases have been reported in the *** SUMMARY Here,we report the case of a 33-year-old man with iron deficiency anemia without manifestation of gastrointestinal *** upper gastrointestinal endoscopy and total colonoscopy were performed,but no abnormalities were found within the observed ***,a capsule endoscopy was performed and offered us a clue to subsequently confirm the diagnosis of inverted Meckel's diverticulum via computed tomography *** intestinal resection surgery was *** final pathology report described a Meckel’s *** Since inverted Meckel's diverticulum is an uncommon disease and its clinical presentation is not specific,it may go undetected by capsule *** diagnosis and treatment of this disease requires a high index of clinical suspicion.

关键词： Inverted Meckel’s diverticulum Capsule endoscopy Anemia study Small bowel tumor Case report

Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Regenerative Biomaterials 2021年第1期8卷 73-82页

作者： Isabel Pereira jose Eduardo Pereira luis Maltez Alexandra Rodrigues Catarina Rodrigues Manuela Oliveira Dina MSilva Ana Rita Caseiro Justina Prada Ana Colette Mauricio jose Domingos Santos Miguel Gama CEB Centre of Biological EngineeingUniversity of MinhoCampus de GualtarBraga 4710-057Portugal CECAV Animal and Veterinary Research CentreUniversity of Trias-os-Montes e Alto DouroVila Real 5001-801Portugal Department of Veterinary Sciences University of Tras-os-Montes e Alto DouroVila Real 5001-801Portugal Bios ckin Molecular and Cell Therapies S.A.Laboratorio CriovidaTecMaiaRua Engenheiro Frederico Ulrich 2650Moreira da Maia 4470-605Portugal Departamento de Clinicas Veterinarias Instituto de Ciencias Biomedicas de Abel Salazar(ICBAS)Universidade do Porto(UP)Rua de Jorge Viterbo Ferreiran°228Porto 4050-313Portugal Centro de Estudos de Ciencia Animal(CECA) Instituto de CienciasTecnologias e Agroambiente da Universidade do Porto(ICETA)Rua D.Manuel IApartado 55142Porto 4051-401 Portugal Centro de Investigaqao Vasco da Gama(CIVG)/Escola Universitaria Vasco da Gama(EUVG) Avenida Jose R.Sousa Femandesn°197 LordemaoCoimbra 3020-210Portugal REQUIMTE/LAQV Departamento de Engenharia Metalurgica e MateriaisFaculdade de EngenhariaUniversidade do PortoRua Dr Roberto FriasPorto 4200-495Portugal

The development of injectable bone substitutes(IBS)have obtained great importance in the bone re-generation field,as a strategy to reach hardly accessible defects using minimally invasive techniques and able to fit to irregular *** this scenario,the association of injectable hydrogels and bone graft granules is emerging as a well-established ***,in situ forming hydrogels have arisen as a new IBS *** in situ forming and injectable dextrin-based hydrogel(HG)was developed,aiming to act as a carrier of granular bone substitutes and bioactive *** this work,the HG was associated to a granular bone substitute(Bonelike)and implanted in goat critical-sized calvarial defects(14mm)for 3,6 and *** results showed that HG improved the han-dling properties of the Bonelike granules and did not affect its osteoconductive features,neither impairing the bone regener ation *** multipotent mesenchymal stromal cells from the umbilical cord,extracellular matrix hydrolysates and the pro-angiogenic peptide LLKKK18 were also combined with the *** bioactive agents did not enhance the new bone formation significantly under the conditions tested,according to micro-computed tomography and histological analysis.

关键词： polysaccharide injec table hydrogel granular ceramics Bonelike' bone regen eration calvarial defect

Molecular cloning and characterization of a lipid transfer protein gene(PsLTP1) from Pinus sylvestris(L.)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Forestry Research 2019年第3期30卷 1149-1158页

作者： Nataliya Hrunyk Valentina Kovaleva Hryhoriy Krynytskyy Ivan Gout Francisco Amil-Ruiz Juan Munoz-Blanco jose luis Caballero Roman Gout Laboratory of Molecular-Genetic Markers in Woody Plants Ukrainian National Forestry UniversityGen. Chuprynka Str. 103Lviv 79057Ukraine Departamento de Bioquimica y Biologia Molecular Campus de Excelencia Internacional Agroalimentario ceiA3-Campus de RabanalesEdificio Severo Ochoa-C6Universidad de Cordoba14071 CordobaSpain Department of Structural and Molecular Biology University College LondonLondon WC1E 6BTUK

Plant nonspecific lipid transfer proteins (nsLTPs) are widely distributed through plant kingdom and are characterized by the presence of a central hydrophobic cavity, suitable for binding various hydrophobic molecules. Despite extensive research on nsLTP in different plant species, mostly angiosperm, and the great diversity of physiological processes in which they seem to be involved, their exact functions still remain unclear. Also, very limited experimental data are available on nsLTP in gymnosperm. In this study, we report for the first time on the molecular cloning of nsLTP, from Pinus sylvestris L.(PsLTP1, GenBank accession JN980402.1) and the expression pattern of PsLTP1 during ontogenesis and in response to environmental stress conditions. Total RNA from roots of 7-day old pine seedlings was used to isolate the cDNA clone, corresponding to Scots pine lipid transfer protein. The open reading frame of PsLTP1 consists of 372 bp encoding a protein of 123 amino acids. Amino acid sequence alignment revealed that mature PsLTP1 shares high level of similarity with nsLTP from other conifers and with well-studied nsLTPs from angiosperms. The PsLTP1 contains a 27-amino-acid N-terminal signal sequence and presents all the features of a plant nsLTP. Amino acid comparison analysis and 3D structure prediction showed that PsLTP1 is a type 1 nsLTP. The results of the expression analysis of Scots pine PsLTP1 gene revealed that its transcripts accumulate in actively growing tissues. Furthermore, transcription of PsLTP1 was upregulated in response to cold and salt treatments, and downregulated during acidic, osmotic and water stresses.

关键词： Scots pine Nonspecific lipid transfer protein (nsLTP) Molecular cloning Expression Abiotic stresses

The different root apex zones contribute to drought priming induced tolerance to a reoccurring drought stress in wheat

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2021年第5期9卷 1088-1097页

作者： Xiao Wang Jing Chen Jiakun Ge Mei Huang Jian Cai Qin Zhou Tingbo Dai luis Alejandro jose Mur Dong Jiang National Technique Innovation Center for Regional Wheat Production/Key Laboratory of Crop Ecophysiology Ministry of Agriculture/Nanjing Agricultural UniversityNanjing 210095JiangsuChina Institute of Biological Environmental and Rural SciencesAberystwyth UniversityAberystwyth SY233DAUK

Drought priming is a promising approach to improve tolerance to further drought in *** root apex plays important roles in drought however,its contribution to drought priming remains *** provide mechanistic insights into this process,the transcriptomes and proteomes at three different zones along the root axis under drought stress were *** assessment of root growth indicated that priming augmented roots growth in response to drought and also the levels of protective proline and glycine *** across the proximal to the distal zones of the root apex indicated increases the transcription of genes involved in primary and secondary ***,genes related to translation,transcription,folding,sorting and degradation,replication and repair were increased in the apex compared to the proximal zone.A single drought episode suppressed their expression but prior drought priming served to maintain expression with recurrent drought *** differentially primed responses genes were mainly involved in the pathways related to plant hormone signaling,stress defense and cell wall *** prediction of regulatory hubs using Cytoscape implicated signaling components such as the ABA receptor PYL4 as influencing antioxidant status and the cell *** our integrative transcriptomic-proteomic assessments we present a model for drought priming protected plant hormone signaling transduction pathways to drive the cell cycle and cell wall loosening to confer beneficial effects on roots to counter the effects of *** model provides a theoretical basis for improvement of drought tolerance in wheat,via an increased understanding of drought priming induced drought tolerance.

关键词： Wheat Drought priming Drought tolerance Root Transcriptome