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Hepatic Expression of Insulin-Like Growth Factor-1 inUnderfed Pregnant Ewes

Journal of Agricultural Science and Technology(A) 2016年第5期6卷 355-362页

作者： Victoria de Brun Ana Meikle Alberto Casal Mariana Carriquiry Claudia Menezes Femando Forcada Cecilia Sosa jose alfonso abecia Laboratory of Animal Endocrinology and Metabolism Veterinary Faculty University of La Repfiblica Lasplaces 1550Montevideo 11600 Uruguay Department of Animal Production and Grazing Agronomy Faculty University of La Republica Garzon 780 Montevideo 12900Uruguay Aragon Environmental Research Institute Department of Animal Production and Food Science University of Zaragoza MiguelServet 177 Zaragoza 50013 Spain

The liver is one of the most important visceral organs, which represents a large contribution to whole animal energyexpenditure and the major synthetic site of insulin-like growth factor-I （IGF-1） peptide. Decreased plane of nutrition acts byreducing the metabolic rate and mass of metabolic tissues, such as liver. Also, undernutrition results in the reduced circulating IGF-1concentrations, due to the uncoupled growth hormone-IGF （GH-IGF） axis. This study investigated whether a 22-day period ofundernutrition （half maintenance） could affect liver mass and IGF-1 protein and gene expression. Sixteen pregnant ewes fed all （n =9） or half （n = 7） of their maintenance energy requirements were slaughtered on day 7 of pregnancy （oestrus = day 0）. Body and livermass, IGF-1 plasmatic concentrations and liver IGF-1 mRNA and protein expression were determined. Liver mass and the proportionof liver mass to empty body weight were lower in underfed animals. While IGF-1 plasmatic concentrations were lower inundernourished ewes, no differences in liver mRNA expression were found. This is the first time that differences inimmunohistochemistry intensity and total content are reported in sheep. In summary, the decreased plasma IGF-I concentrationsinduced by undernutrition in ewes was not associated with its reduced hepatic mRNA or protein expression, but to a decrease in livermass.

关键词： Undemutrition, insulin-like growth factor-l, liver mass, sheep.

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Rheological and tribological approaches as a tool for the development of sustainable lubricating greases based on nano-montmorillonite and castor oil

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Friction 2021年第2期9卷 415-428页

作者： jose Enrique MARTÍN-alfonso María jose MARTÍN-alfonso Concepción VALENCIA María Teresa CUBERES Department of Chemical Engineering and Materials Science Campus de“El Carmen”University of HuelvaChemical Product and Process Technology Research Center(Pro2TecS)Huelva 21071Spain Department of Applied Mechanics and Project Engineering University of Castilla-La ManchaAlmadén 13400Spain

This study investigates the development of novel montmorillonite/castor oil blends to formulate sustainable lubricating greases to promote the replacement of petrochemicals industry-derived materials by substances obtained from renewable ***,the effect of the thickener concentration on the rheological,chemical,thermal,tribological properties,and atomic force microscopy(AFM)microstructure of these systems were *** results showed that the C20A nanoclay content could be used to modulate the viscosity values,the linear viscoelastic functions,and tribological properties of these montmorillonite *** general,these gel-like dispersions exhibited remarkable lubricant properties;the samples showed values of the friction coefficient and wear scars similar or lower than those obtained with model bentonite grease.

关键词： lubricating greases nanoclay vegetable oil rheology tribology

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Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

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Asian Journal of Andrology 2010年第3期12卷 431-441,I0012页

作者： Juan Carlos Martinez-Soto Juan de DiosHourcade alfonso Gutierrez-Adan jose Lorenzo Landeras Joaquin Gadea Department of Physiology School of Veterinary Murcia University Murcia 30100 Spain IVI- MURCIA Murcia 30007 Spain Department of Animal Reproduction INIA Madrid 28040 Spain

In this study, we evaluated the effects of genistein supplementation of the thawing extender on frozen-thawed human semen parameters. We analyzed the effect of supplementation on sperm motility, capacitation （membrane lipid disorder）, reactive oxygen species （ROS） generation, chromatin condensation and DNA damage. Using this preliminary information, it maybe possible to improve the cryopreservation process and reduce the cellular damage. We have confirmed that the isoflavone genistein （10 μmol L-1） has antioxidant properties on the frozen-thawed spermatozoa. This results in a decreased ROS production that shows a slight improvement in the sperm motility, and decreases the membrane lipid disorder and DNA damage caused by cryopreservation. These results suggest an effect of genistein on sperm functionality that could be of interest for assisted reproduction treatments using frozen- thawed human spermatozoa, but further studies will be necessary to confirm our findings and to evaluate the possible clinical applications.

关键词： antioxidants cryopreservation DNA damage genistein isoflavones sperm motility

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Repeatability of in-vitro optical quality measurements of intraocular lenses with a deflectometry technique effect of the toricity

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International Journal of Ophthalmology(English edition) 2018年第7期11卷 1139-1144页

作者： Teresa Ferrer-Blasco Alberto Dominguez-Vicent Santiago Garcia-Lazaro Maria Amparo Diez-Ajenjo jose F.alfonso jose J.Esteve-Taboada Department of Optics and Optometry and Visual Science University of Valencia Valencia 46100 Spain Clinica Optometrica Fundacio Lluis Alcanyis Universitat de Valencia Valencia 46020 Spain Surgery Department Fernandez-Vega Ophthalmological Institute Oviedo 33012 Spain School of Medicine University of Oviedo Oviedo 33006 Spain

AIM： To evaluate the repeatability of an optical device for measuring the Zernike coefficients of toric intraocular lenses（IOLs） and assess whether its toricity has any impact in its repeatability. METHODS： An experienced technician used the NIMO TR1504 to measure the Zernike coefficients 30 times for an aperture of 4.50 mm for all lenses included. The IOLs included were divided into two group： toric and nontoric ones. The cylindrical powers of the toric lenses included in the present study were 1.00, 1.50, 2.25, 3.00 and 3.75 D. Finally, the repeatability of the NIMO TR1504 was described in terms of within subject standard deviation（Sw） and repeatability limit. RESULTS： The Sw was smaller than 0.011 μm for both lens groups and all Zernike coefficients, and the difference between both groups was smaller than 0.004 μm for all Zernike coefficients. Regarding the repeatability limit, this value was smaller than 0.025 μm for the toric lens group, and smaller than 0.031 μm for the non-toric lens one for all Zernike coefficients. Furthermore, the maximum difference between both lens groups was 0.010 μm. CONCLUSION： The repeatability of the NIMO TR1504 to measure the optical quality is high and independent of the lens toricity. These results reflect that this system is robust and could be used to measure the in-vitro optical quality of either toric or non-toric IOLs.

关键词： in-vitro measurements optical quality intraocular lens repeatability

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Tribological performance of tributylmethylammonium bis(trifluoromethylsulfonyl)amide as neat lubricant and as an additive in a polar oil

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Friction 2019年第3期7卷 282-288页

作者： Rubén GONZáLEZ Diego RAMOS David BLANCO alfonso FERNáNDEZ-GONZáLEZ jose L.VIESCA Mark HADFIELD Antolin HERNáNDEZ BATTEZ Department of Marine Science and Technology University of OviedoAsturias 33203Spain Department of Construction and Manufacturing Engineering University of OviedoAsturias 33203Spain Department of Physical and Analytical Chemistry University of OviedoAsturias 33006Spain Department of Design and Engineering Bournemouth UniversityPoole BH12 5BBUK

The ionic liquid(IL) tributylmethylammonium bis(trifluoromethylsulfonyl)amide([N4441][NTf2]) was used as neat lubricant and as an additive(1.5 wt%) in a polar oil to study its friction and wear reducing *** tests were completed for 90 minutes at room temperature and 100 °C in a reciprocating configuration at loads of 30 and 70 N,10 Hz-frequency,and 4 mm stroke *** volume was measured by confocal microscopy and the surface-IL interaction determined by *** main findings were that neat IL showed the best tribological behavior;the IL-containing mixture behaved similar to the base oil regarding friction,however outperformed the antiwear behavior of the base oil under higher temperature;surface-IL chemical interaction was found mainly at 100 °C.

关键词： ionic liquid additive friction wear polar oil

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Pre-fire aboveground biomass, estimated from LiDAR, spectral and field inventory data, as a major driver of burn severity in maritime pine (Pinus pinaster) ecosystems

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Forest Ecosystems 2022年第2期9卷 234-246页

作者： jose Manuel Fernandez-Guisuraga Susana Suarez-Seoane Paulo MFernandes Victor Fernandez-Garcia alfonso Fernandez-Manso Carmen Quintano Leonor Calvo Area of Ecology Department of Biodiversity and Environmental ManagementFaculty of Biological and Environmental SciencesUniversity of Leon24071LeonSpain Centro de Investigacao e de Tecnologias Agroambientais e Biologicas Universidade de Tras-os-Montes e Alto Douro5000-801Vila RealPortugal Department of Organisms and Systems Biology(Ecology Unit)and Research Unit of Biodiversity(IMIB UO-CSIC-PA)University of OviedoOviedoMieresSpain Agrarian Science and Engineering Department School of Agricultural and Forestry EngineeringUniversity of Leon24400PonferradaSpain Electronic Technology Department School of Industrial EngineeringUniversity of Valladolid47011ValladolidSpain Sustainable Forest Management Research Institute University of Valladolid-Spanish National Institute for Agriculture and Food Research and Technology(INIA)34004PalenciaSpain

Background:The characterization of surface and canopy fuel loadings in fire-prone pine ecosystems is critical for understanding fire behavior and anticipating the most harmful ecological effects of ***,the joint consideration of both overstory and understory strata in burn severity assessments is often *** aim of this work was to assess the role of total,overstory and understory pre-fire aboveground biomass(AGB),estimated by means of airborne Light Detection and Ranging(LiDAR)and Landsat data,as drivers of burn severity in a megafire occurred in a pine ecosystem dominated by Pinus pinaster *** the western Mediterranean ***:Total and overstory AGB were more accurately estimated(R^(2) equal to 0.72 and 0.68,respectively)from LiDAR and spectral data than understory AGB(R^(2)=0.26).Density and height percentile LiDAR metrics for several strata were found to be important predictors of *** severity responded markedly and non-linearly to total(R^(2)=0.60)and overstory(R ^(2)=0.53)AGB,whereas the relationship with understory AGB was weaker(R ^(2)=0.21).Nevertheless,the overstory plus understory AGB contribution led to the highest ability to predict burn severity(RMSE=122.46 in dNBR scale),instead of the joint consideration as total AGB(RMSE=158.41).Conclusions:This study novelty evaluated the potential of pre-fire AGB,as a vegetation biophysical property derived from LiDAR,spectral and field plot inventory data,for predicting burn severity,separating the contri-bution of the fuel loads in the understory and overstory strata in Pinus pinaster *** evidenced relationships between burn severity and pre-fire AGB distribution in Pinus pinaster stands would allow the implementation of threshold criteria to support decision making in fuel treatments designed to minimize crown fire hazard.

关键词： Aboveground biomass Burn severity Landsat LiDAR Pinus pinaster

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Electrifying Ba_(0.5)Sr_(0.5)Co_(0.8)Fe_(0.2)O_(3-δ) for focalized heating in oxygen transport membranes

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Journal of Energy Chemistry 2024年第4期91卷 99-110页

作者： Marwan Laqdiem Julio García-Fayos Laura Almar alfonso J.Carrillo Álvaro Represa JoséM.López Nieto Sonia Escolástico David Catalán-Martinez jose M.Serra Instituto de Tecnología Química(Universitat Politècnica de València–Consejo Superior de Investigaciones Científicas) Av.Los Naranjos s/nE-46022 ValenciaSpain

Industry decarbonization requires the development of highly efficient and flexible technologies relying on renewable energy resources,especially biomass and solar/wind *** the case of pure oxygen production,oxygen transport membranes(OTMs)appear as an alternative technology for the cryogenic distillation of air,the industrially-established process of producing ***,OTMs could provide oxygen from different sources(air,water,CO_(2),etc.),and they are more flexible in adapting to current processes,producing oxygen at 700^(-1)000℃.Furthermore,OTMs can be integrated into catalytic membrane reactors,providing new pathways for different *** first part of this study was focused on electrification on a traditional OTM material(Ba_(0.5)Sr_(0.5)Co_(0.8)Fe_(0.2)O_(3-δ)),imposing different electric currents/voltages along a capillary *** to the emerging Joule effect,the membrane-surface temperature and the associated O_(2) permeation flux could be ***,the OTM is electrically and locally heated and reaches 900℃on the surface,whereas the surrounding of the membrane was maintained at 650℃.The O_(2)permeation flux reached for the electrified membranes was~3.7 NmL min^(-1)cm^(-2),corresponding to the flux obtained with an OTM non-electrified at 900℃.The influence of depositing a porous Ce_(0.8)Tb_(0.2)O_(2-δ) catalytic/protective layer on the outer membrane surface revealed that lower surface temperatures(830℃)were detected at the same imposed electric ***,the electrification concept was demonstrated in a catalytic membrane reactor(CMR)where the oxidative dehydrogenation of ethane(ODHE)was carried *** reaction is very sensitive to temperature,and here,we demonstrate an improvement of the ethylene yield by reaching moderate temperatures in the reaction chamber while the O_(2) injection into the reaction can be easily fine-tuned.

关键词： Oxygen permeation Oxidative dehydrogenation of ethane Oxygen transport membranes Joule effect Mixed ionic-electronic conductors Catalytic membrane reactors

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Intracameral 5-fluorouracil and viscous dispersive viscoelastic for diffuse epithelial downgrowth management in aphakia

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International Journal of Ophthalmology(English edition) 2020年第5期13卷 845-847页

作者： Santiago Montolío-Marzo Ester Fernández-López jose Vicente Piá-Ludena Juan María Davó-Cabrera Enrique Antonio alfonso-Munoz Cristina Peris-Martínez FISABIO Oftalmología Médica Valencia 46015Spain

Dear Editor,I am *** Montolío-Marzo from FISABIO Oftalmología Médica,Valencia,Spain.I write you to present a case report of epithelial downgrowth in an aphakic *** epithelial downgrowth is one of the most feared complications in cases of penetrating ocular trauma or recurrent ocular *** progress can affect all the ocular structures,giving rise to corneal decompensation,retinal detachment,difficult-to-treat glaucoma or phthisis bulbi and many cases end in enucleation[1].Fortunately,the incidence of this condition is very low,there being between 0.08%and 0.12%after cataract surgery and 0.25%after penetrating *** infrequency means that scientific evidence is reduced to case series and the opinions of experts without there being a homogeneous criterion to guide the work of the doctor in *** these reasons,epithelial downgrowth is still a diagnostic and therapeutic challenge.

关键词： viscoelastic viscous camera

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A new oxidative pathway of nitric oxide production from oximes in plants

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Molecular Plant 2024年第1期17卷 178-198页

作者： Pedro Lopez-Gomez Javier Buezo Marina Urra alfonso Cornejo Raquel Esteban Jorge Fernandez de los Reyes Estibaliz Urarte Estefania Rodriguez-Dobreva Alejandro Chamizo-Ampudia Alejandro Eguaras Sebastian Wolf Daniel Marino Victor Martinez-Merino jose F.Moran Institute for Multidisciplinary Research in Applied Biology(IMAB) Department of SciencesPublic University of Navarre(UPNA)Avda.de Pamplona 12331192 MutilvaSpain Institute for Advanced Materials and Mathematics(INAMAT2) Department of SciencesPublic University of Navarre(UPNA)Campus de Arosadia31006 PamplonaSpain Department of Plant Biology and Ecology University of the Basque Country(UPV/EHU)Sarriena s/nApdo.64448080 BilbaoSpain Center for Plant Molecular Biology(ZMBP) University of TubingenGeschwister-Scholl-Platz72074 TubingenGermany Institute of Natural Resources and Agrobiology of Salamanca(IRNASA) Department of Plant-Microorganism InteractionsSpanish National Research Council(CSIC)Cordel de Merinas40-5237008SalamancaSpain Department of Biochemistry Area of Molecular BiologyVeterinary FacultyUniversity of LeonCampus de Vegazanas/n.24071 LeonSpain

Nitric oxide(NO)is an essential reactive oxygen species and a signal molecule in *** several studies have proposed the occurrence of oxidative NO production,only reductive routes for NO production,such as the nitrate(NO_(3)^(-))-upper-reductase pathway,have been evidenced to date in land ***,plants grown axenically with ammonium as the sole source of nitrogen exhibit contents of nitrite and NO3−,evidencing the existence of a metabolic pathway for oxidative production of *** hypothesized that oximes,such as indole-3-acetaldoxime(IAOx),a precursor to indole-3-acetic acid,are intermediate oxidation products in NO *** detected the production of NO from IAOx and other oximes catalyzed by peroxidase(POD)enzyme using both 4-amino-5-methylamino-2′,7′-difluorescein fluorescence and *** stimulated the reaction,while superoxide dismutase inhibited ***,mouse NO synthase can also use IAOx to produce NO at a lower rate than *** provided a full mechanism for POD-dependent NO production from IAOx consistent with the experimental data and supported by density functional theory *** showed that the addition of IAOx to extracts from Medicago truncatula increased the in vitro production of NO,while in vivo supplementation of IAOx and other oximes increased the number of lateral roots,as shown for NO donors,and a more than 10-fold increase in IAOx dehydratase ***,we found that in vivo supplementation of IAOx increased NO production in Arabidopsis thaliana wild-type plants,while prx33-34 mutant plants,defective in POD33-34,had reduced *** data show that the release of NO by IAOx,as well as its auxinic effect,explain the superroot ***,our study reveals that plants produce NO utilizing diverse molecules such as oximes,POD,and flavins,which are widely distributed in the plant kingdom,thus introducing a long-awaited oxidative pathway to NO production in plants.T

关键词： ammonium oxidation auxin nitric oxide oxime peroxidase root phenotype

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Wound healing reaction:A switch from gestation to senescence

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World Journal of Experimental Medicine 2014年第2期4卷 16-26页

作者： Maria-Angeles Aller jose-Ignacio Arias Luis-alfonso Arraez-Aybar Carlos Gilsanz Jaime Arias Surgery Department School of MedicineComplutense University of Madrid28040 MadridSpain General Surgery Unit Monte Naranco Hospital Monte Naranco Hospital OviedoAsturias33012 OviedoSpain Department of Human Anatomy and Embryology Ⅱ School of MedicineComplutense University of Madrid28040 MadridSpain General Surgery Unit Sureste HospitalArganda del Rey28040 MadridSpain

The repair of wounded tissue during postnatal life could be associated with the upregulation of some functions characteristic of the initial phases of embryonic development. The focusing of these recapitulated systemic functions in the interstitial space of the injured tissue is established through a heterogeneous endothelial barrier which has excretory-secretory abilities which in turn,would induce a gastrulation-like process. The repair of adult tissues using upregulated embryonic mechanisms could explain the universality of the inflammatory response against injury,regardless of its etiology. However,the early activation after the injury of embryonic mechanisms does not always guarantee tissue regeneration since their long-term execution is mediated by the host organism.

关键词： Wound healing Repair Embryonic mechanisms Vitelline Amniotic

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