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色谱 2012年第11期30卷 1194-1202页

作者： André Moreni LOPEs jorge sánchez romeu Rolando Páez MEIRELEs Gabriel Marquez PERERA Rolando Perdomo MORALEs Adalberto PEssOA Lourdes Zumalacárregui CáRDENAs Department of Biochemical and Pharmaceutical Technology School of Pharmaceutical SciencesUniversity of So Paulo(FCF/USP) Purification Development Department Center for Genetic Engineering and Biotechnology(CIGB) Center for Pharmaceuticals Research and Development(CIDEM) Higher Polytechnic Institute José Antonio Echeverría(CUJAE)

Endotoxins(also known as lipopolysaccharides(LPs)) are undesirable by-products of recombinant proteins,purified from Escherichia *** can be considered stable under a wide range of temperature and pH,making their removal one of the most difficult tasks in downstream processes during protein *** inherent toxicity of LPs makes their removal an important step for the application of these proteins in several biological assays and for a safe parenteral *** metal affinity chromatography(IMAC) enables the affinity interactions between the metal ions(immobilized on the support through the chelating compound) and the target molecules,thus enabling high-efficiency separation of the target molecules from other components present in a *** chromatography is applied with Ca2+-iminodiacetic acid(IDA) to remove most of the LPs contaminants from the end product(more than90%).In this study,the adsorption of LPs on an IDA-Ca2+ was *** adsorption Freundlich isotherm of LPs-IDA-Ca2+ provides a theoretical basis for LPs *** was found that LPs is bound mainly by interactions between the phosphate group in LPs and Ca2+ ligands on the *** factors such as pH(4.0 or 5.5) and ionic strength(1.0 mol/L) are essential to obtain effective removal of LPs for contaminant levels between endotoxin' concentration values less than100 EU/mL and 100 000 EU/*** new protocol represents a substantial advantage in time,effort,and production costs.

关键词： immobilized metal affinity chromatography(IMAC) lipopolysaccharides(LPs) endotoxin removal recombinant proteins isotherms protein purification

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Targeting harmful effects of non-excitatory amino acids as an alternative therapeutic strategy to reduce ischemic damage

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Neural Regeneration Research 2025年第9期20卷 2454-2463页

作者： Victoria Jiménez Carretero IrisÁlvarez-Merz jorge Hernández-Campano sergei A.Kirov Jesús M.Hernández-Guijo Department of Pharmacology and Therapeutic School of MedicineUniv.Autónoma de MadridMadridSpain Ramón y Cajal Institute for Health Research(IRYCIs) Neurobiology-Research ServiceHospital Ramón y CajalMadridSpain Institute of Neurobiology Faculty of Mathematics and Natural SciencesHeinrich Heine University DüsseldorfDüsseldorfGermany Department of Neuroscience and Regenerative Medicine&Department of Neurosurgery Medical College of Georgia at Augusta UniversityAugustaGAUSA

The involvement of the excitatory amino acids glutamate and aspartate in ce rebral ischemia and excitotoxicity is ***,the role of non-excitatory amino acids in brain damage following a stroke or brain trauma remains largely *** release of amino acids by necrotic cells in the ischemic core may contribute to the expansion of the *** findings indicated that the reversible loss of field excitato ry postsynaptic potentials caused by transient hypoxia became irreversible when exposed to a mixture of just four non-excitatory amino acids(L-alanine,glycine,L-glutamine,and L-serine)at their plasma *** amino acids induce swelling in the somas of neurons and astrocytes during hypoxia,along with permanent dendritic damage mediated by N-methyl-D-aspartate *** N-methyl-D-aspartate receptors prevented neuronal damage in the presence of these amino acids during *** is likely that astroglial swelling caused by the accumulation of these amino acids via the alanine-serine-cysteine transporter 2 exchanger and system N transporters activates volume-regulated anion channels,leading to the release of excitotoxins and subsequent neuronal damage through N-methyl-D-aspartate receptor ***,previously unrecognized mechanisms involving non-excitatory amino acids may contribute to the progression and expansion of brain injury in neurological emergencies such as stroke and traumatic brain *** these pathways co uld highlight new therapeutic targets to mitigate brain injury.

关键词： cell swelling N-methyl-D-aspartate receptor non-excitatory amino acids stroke synaptic transmission

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Endoscopic techniques for the diagnosis of pancreatic cystic lesions

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World Journal of Gastroenterology 2025年第1期31卷 1-7页

作者： sahib singh saurabh Chandan Rakesh Vinayek Jahnvi Dhar Jayanta samanta Gabriele Capurso Ivo Boskoski Cristiano spada jorge D Machicado stefano Francesco Crinò Antonio Facciorusso Department of Internal Medicine Sinai HospitalBaltimoreMD 21215United States Division of Gastroenterology and Hepatology Creighton University School of MedicineOmahaNE 68131United States Department of Gastroenterology Sinai Hospital of BaltimoreBaltimoreMD 21215United States Department of Gastroenterology Postgraduate Institute of Medical Education and ResearchChandigarh 160012India Division of Pancreato-Biliary Endoscopy and Endosonography Pancreas Translational and Clinical Research CenterIRCCS San Raffaele Scientific InstituteVita-Salute San Raffaele UniversityMilano 20132Italy Digestive Endoscopy Unit Fondazione Policlinico Universitario Agostino Gemelli IRCSSUniversitàCattolica del Sacro Cuore di RomaCenter for Endoscopic Research Therapeutics and TrainingRoma 00168Italy Department of Gastroenterology and Endoscopy Catholic UniversityRoma 00168Italy Department of Gastroenterology University of Michigan HealthAnn ArborMI 48107United States Department of Endoscopy University of VeronaVerona 37121VenetoItaly Gastroenterology Unit Department of Medical and Surgical SciencesUniversity of FoggiaFoggia 71122Italy

Pancreatic cysts are mostly incidental findings on computed tomography or magnetic resonance imaging scans,with few patients presenting with abdominal pain or other *** accurate diagnosis of cysts is important as management depends on the type(neoplastic or non-neoplastic).Cross-sectional imaging is fast being replaced with endoscopic ultrasound(EUs)and various techniques based on that such as EUs-guided fine needle aspiration,EUs-guided needle confocal laser endomicroscopy,EUs-through-the-needle biopsy,and contrast-enhanced *** studies have reported varying diagnostic and adverse event rates with these *** addition,American,European,and Kyoto guidelines for the diagnosis and management of pancreatic cysts have provided different *** this editorial,we elaborate on the clinical guidelines,recent studies,and comparison of different endoscopic methods for the diagnosis of pancreatic cysts.

关键词： Endoscopic ultrasound Fine needle aspiration Needle confocal laser endomicroscopy Through-the-needle biopsy Contrast-enhanced endoscopic ultrasound

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Ultrasonic spray pyrolysis deposition of snse and snse_2 using a single spray solution

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Journal of semiconductors 2013年第1期34卷 12-15页

作者： jorge sergio Narro-Rios Manoj Ramachandran Dalia Martínez-Escobar Aarón snchez-Jurez Centro de investigación en Energía UNAMP.O.Box 3462580 TemixcoMorelosMxico

Thin films of snse and snse2 have been deposited using the ultrasonic spray pyrolysis（UsP） *** the best of our knowledge this is the first report of the deposition of snse and snse2 thin films using a single spray *** use of a single spray solution for obtaining both a p-type material,snse,and an n-type material,snse2,simplifies the deposition *** snse2 thin films have a bandgap of 1.1 eV and the snse thin films have a band gap of 0.9 *** Hall measurements were used to determine the resistivity of the thin *** snse2 thin films show a resistivity of 36.73 Ωcm and n-type conductivity while the snse thin films show a resistivity of 180 Ωcm and p-type conductivity.

关键词： snse snse2 ultrasonic spray pyrolysis technique

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Non-Canonical Functions of the E2F/DP Pathway with Emphasis in Plants

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Phyton-International Journal of Experimental Botany 2021年第2期90卷 307-330页

作者： Víctor A.sánchez-Camargo samantha Romero-Rodríguez jorge M.Vázquez-Ramos Facultad de Química Departamento de BioquímicaUniversidad Nacional Autónoma de MéxicoAvenida Universidad y CopilcoCiudad de México04510México Plant Development and(Epi)Genetics Group Swammerdam Institute for Life SciencesUniversiteit van AmsterdamAmsterdam1098XHNetherlands

The E2F/DP pathway is a widely conserved regulatory mechanism in pluricellular *** family of E2F and DP transcription factors was originally described having a role in the transition from the G1 to the s phase of the cell ***,the discovery of hundreds of possible gene targets and their involvement in many other biochemical processes,soon showed that they participated in cell development and differentiation,chromatin remodeling,DNA repair and *** E2F/DP transcription factors can act as either activators or repressors of transcription depending on their association to other regulatory proteins,particularly the retinoblastoma protein,or even depending to their protein structure that can define their *** plants the E2F/DP pathway also regulates endoreduplication,a process present along the life cycle,from organ elongation to root differentiation and *** transcription factors also help plant cells to respond to environmental disturbances such as those caused by different types of radiation,or by *** review focuses on the“so called”non-canonical functions of the E2F/DP family proteins in animal and plant cells,that are in fact essential activities that connect regulatory circuits among multiple metabolic pathways by means of their atypical functions.

关键词： E2F DP transcription factors atypical functions plant cells

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Biodiversity, dynamics, and impact of chakras on the Ecuadorian Amazon

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Journal of Plant Ecology 2019年第1期12卷 34-44页

作者： Roy R.Vera V J.Hugo Cota-sánchez jorge E.Grijalva Olmedo University of saskatchewan Department of Biology112 Science PlaceSaskatoon SK S7N 5E2Canada University of saskatchewan Department of Biology and W.P.Fraser Herbarium(SASK)112 Science PlaceSaskatoon SK S7N 5E2Canada Universidad Central del Ecuador Facultad de Medicina Veterinaria y ZootecniaJerónimo Leiton y Gatto SobralQuito 170521Ecuador Estación Experimental santa Catalina Panamericana Sur Km1Instituto Nacional de Investigaciones AgropecuariasMejía 171101Ecuador

Aims Deforestation and biodiversity loss are two alarming,closely related problems,and the main factors triggering changes in land *** agricultural practices in the western Amazon Basin are known as chakras,and their structure and dynamics are seemingly optimal for forest ***,the variability in tree spe-cies and the degree of forest recovery after abandonment is poorly documented in this agroforestry system(AFs).The goals of this study were:(i)to investigate whether the different AFss(chakras)preserve similar levels of forest diversity,(ii)to determine the effect of trans-formation of mature forests(MF)to chakras,in particular,forest alpha and beta diversity levels,and(iii)to investigate whether native tree species recovery leads to the original forest structure following chakra *** We assessed the floristic composition in three AFss(cassava,corn,and cocoa),the secondary forest(sF),and the forest remnants in the buffer zone of the Northern Ecuadorian Amazon(NEA).All tree spe-cies with a diameter at breast height(dbh)≥10 cm were inventoried in 61 plots(0.28 ha average)representing 17.44 *** diversity was calculated in all systems to determine the levels of variabil-ity using species richness and the shannon diversity ***,beta diversity was examined to evaluate the degree of dissimilarity among all AFss with the MF in order to analyze changes in floristic *** divergence between the sF and the MF was ana-lyzed to ascertain forest recovery after chakra *** Findings A total of 4,060 trees(dbh≥10 cm)representing 109 species,96 genera,and 43 plant families were inventoried in 17.44 ha sampled in five systems in the buffer zone of the *** most dominant plant families were Arecaceae,Myristicaceae,Fabaceae,Meliaceae,and Malvaceae,and the most representative genera included Iriartea,Virola,Guarea,Ocotea,Cordia,Chrysophyllum,and *** MF in this zone is composed of 81 tree species circums

关键词： agroforestry biodiversity chakra climate change tropical forest

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sticky-MARTINI as a reactive coarse-grained model for molecular dynamics simulations of silica polymerization

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npj Computational Materials 2022年第1期8卷 467-479页

作者： AndréP.Carvalho sérgio M.santos Germán Pérez-sánchez JoséD.Gouveia JoséR.B.Gomes Miguel jorge CICECO-Aveiro Institute of Materials Department of ChemistryUniversity of AveiroCampus Universitário de Santiago3810-193AveiroPortugal Department of Chemical and Process Engineering University of Strathclyde75 Montrose StreetGlasgowG11XJUK

We report a molecular modeling paradigm to describe silica polymerization reactions in aqueous solutions at conditions that are representative of realistic experimental processes like biosilicification or porous silica synthesis–*** close to ambient temperatures and over a wide range of *** key point is to describe the si-O-si chemical bond formation and breakage processes through a continuous potential with a balance between attractive and repulsive interactions between suitably placed virtual sites and sticky *** simplicity of the model,its applicability in standard parallelized molecular dynamics codes,and its compatibility with the widely used MARTINI coarse-grained force-field allows for the study of systems containing millions of atoms over microsecond time *** model is calibrated to match experimental results for the temporal evolution of silica polymerization in aqueous solution close to the isoelectric point,and can describe silica polymerization and self-assembly processes during encapsulation of a surfactant micelle.

关键词： silica polymerization dynamics

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Arrhythmogenic cardiomyopathy with left ventricular involvement versus ischemic heart disease: lessons learned from the family study and the reviewed autopsy of a young male

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Forensic sciences Research 2019年第3期4卷 274-279页

作者： Pilar Molina jorge sanz-sánchez Manuel Fenollosa Marina Martínez-Matilla Juan Giner Esther Zorio servicio de Patología Instituto de Medicina Legal y Ciencias Forenses de ValenciaValenciaEspana Unidad de Valoración del Riesgo de Muerte súbita Familiar and Unidad de Cardiopatías Familiares Muerte Súbita y Mecanismos de Enfermedad(CaFaMuSMe)Instituto de Investigación Sanitaria La FeValenciaEspana servicio de Cardiología Hospital Universitario y Politécnico La FeValenciaEspana Unidad Genómica Instituto de Investigación Sanitaria La FeValenciaEspana

Ischemic heart disease (IHD) is the leading cause of sudden cardiac death (sCD) and often non-thrombosed severe coronary stenoses with or without myocardial scars are *** dominant arrhythmogenic cardiomyopathy (LDAC) is a life-threating rare disease which has been more thoroughly studied in the last *** macroscopic study of an sCD victim was conducted and re-evaluated 9years *** cardiological work-up in his firstdegree relatives initially comprised an electrocardiogram (ECG) and an *** they were re-evaluted 9years later,a cardiac magnetic resonance,an ECG-monitoring,an exercise testing and a genetic study were performed and the pedigree was extended *** 2008,an IHD was suspected in the sports-triggered sCD of a 37-year-old man upon the postmortem (75% stenosis of the left main and circumflex coronary arteries;the subepicardial left ventricular fibrofatty infiltration with mild myocardial degeneration was assumed to be a past myocardial infarction).No cardiomyopathy was identified in any of the two proband's *** years thereafter,distant relatives were diagnosed with LDAC due to a pathogenic desmoplakin *** reanalysis of the two sisters showed ventricular arrhythmias in one of them without structural heart involvement and the reviewed postmortem of the proband was reclassified as LDAC based on the fibrofatty infiltration;both were mutation *** completion of the family study on 19 family members yielded one sCD due to LDAC (the proband),three living patients diagnosed with LDAC (two with a defibrillator),one mutation carrier without structural ventricular involvement,and 14 healthy relatives (who were discharged) with a very good co-segregation of the *** rare,LDAC exists and sometimes its differential diagnosis with iHD has to be *** previous postmortem misdiagnoses can help family screening to further prevent sCDs.

关键词： Forensic sciences forensic pathology ischemic heart disease arrhythmogenic cardiomyopathy family study

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Avian responses to an extreme weather event:The case of the‘Filomena’snowstorm in Madrid(central spain)

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Avian Research 2024年第2期15卷 180-186页

作者： Carlos Lazaro Carlos sanchez-García Fabian Casas E.jorge Tizado Department of Research Fundacion Artemisan13001 Ciudad RealSpain Department of Zoology Faculty of SciencesUniversity of GranadaGranadaSpain Department of Biodiversity and Environmental Management Universidad de Leon24401 PonferradaSpain

Cold spells and severe weather may have detrimental effects in wild birds,but owing to the stochastic nature of these events,it is not always possible to measure their *** January 2021,a strong snowstorm known as‘Filomena’affected spain,mainly the region of Madrid(Central spain),which was fully covered with snow and suffered freezing temperatures for a *** bird surveys conducted in the winter-spring of 2020 and 2021(before and after Filomena)at same locations,we addressed the possible impact of the cold spell in three widespread gamebirds:Wood Pigeon(Columba palumbus),Eurasian Magpie(Pica pica)and Red-legged Partridge(Alectoris rufa).No significant abundance differences were found between years for none of the species and hunting pressure was only significantly reduced for Partridges,hence this species may have been negatively affected by *** suggest that in response to Filomena,Pigeons conducted partial migration in search of better conditions,while Magpies aggregated in certain areas of Madrid and Partridges endured the harsh conditions within their home *** results highlight the importance to alleviate negative impacts of extreme weather events in wild birds through management actions,especially for sedentary species.

关键词： Alectoris rufa Climate change Cold spell Columba palumbus Migration Pica pica Winter

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OPTIMAL WINDOW GEOMETRY FACTORs FOR ELEMENTARY sCHOOL BUILDINGs IN PORTUGAL

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Journal of Green Building 2018年第1期13卷 185-197页

作者： jorge s.Carlos Centre of Materials and Building Technologies(CMADE-UBI) Universidade da Beira InteriorRua Marquês D’Ávila e Bolama6200-001CovilhãPortugal

INTRODUCTION With respect to thermal performance, windows are the weakest component of the building envelope, essentially because the U-value is usually higher than the opaque envelope. This would allow the highest heat conductance of the building envelope. However, it also helps buildings to gain useful solar heat during winter. Therefore, it has been generally accepted that passive buildings would have small windows towards the poles and large windows facing the equator (Persson, Roos, and Wall 2006). In spite of this guideline, large or fully glazed facades have been used in modern architecture. The intensive use of air conditioning is the result of overheating and high thermal loss problems, which otherwise would lead to thermal discomfort. This extensive use of large windows associated with high energy consumption has motivated researchers to study this building component. Window areas were investigated by Persson et al. (Persson, Roos, and Wall 2006) on 20 terraced houses with larger windows facing the equator and built in Gothenburg. The building envelope was well insulated and fitted with energy efficient windows. It was found that energy efficient windows do not have a major influ-ence on the heating demand in the winter, but it is relevant for the cooling need in summer. Therefore, reduced indoor illuminance due to small windows can be solved by enlarging them in order to obtain relevant daylighting conditions. When efficient windows are designed for a warm climate, as in Mexico, reducing heat flux and solar transmittance indoors was the best option for energy savings (Aguilar et al. 2017). However, reducing solar transmittance influences the indoor illuminance, which was not analyzed.

关键词： school building classroom windows solar heat gain daylight

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