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Early Detection of White Spot Syndrome Virus(WSSV)in Isolated Hemocytes of Litopenaeus vannamei

CellBio 2017年第1期6卷 1-12页

作者： Ariadne hernandez-Perez Rossanna Rodriguez-Canul Edgar Torres-Irineo Fernando Mendoza-Cano Daniel Eduardo Coronado-Molina Jesus Alejandro Zamora-Briseno jorge hernandez-lopez Laboratorio de Inmunología y Biología Molecular Centro de Investigación y de Estudios Avanzados del IPN(CINVESTAV-IPN)Unidad MéridaMéridaMéxico Laboratorio de Pesquerías Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional-Unidad MéridaMéridaMéxico UMDI-Sisal Facultad de CienciasUniversidad Nacional Autónoma de MéxicoSierra PapacalMéxico Centro de Investigaciones Biológicas del Noroeste HermosilloMéxico

To date, White Spot Syndrome (WSS) produced by the White Spot Syndrome Virus (WSSV) causes one of the most severe diseases infecting penaeid shrimps worldwide. Although a vast amount of studies has elucidated pathogenesis in live infection models, there is still little information about the interaction of WSSV infections using in vitro models in the whiteleg shrimp Litopenaeus vannamei (L. vannamei) hemocytes. In this study, a WSSV infection kinetics was performed using total hemocytes isolated from healthy L. vannamei organisms and maintained in in vitro conditions using isotonic solution for shrimp (ISS). The infected experimental cells received ≈ 30,000 viral copies of WSSV. The viability of the hemocytes (control and infected group) was measured during the kinetics with trypan blue exclusion method and cells were maintained up to 6 hpi (post-infection) with non-significant differences of viability between both groups. WSSV replication was assessed using RT- PCR at the RNA expression level of the early viral gene Ie1 and transcripts were detected as early as 30 min pi. Hemocytes from WSSV group showed disrupted integrity, degranulation and irregular shape. This study provides evidence of the capability of WSSV to infect and replicates in L. vannamei hemocytes using in vitro assays in short times as 30 min.

关键词： WSSV Litopenaeus vannamei Ie1 Gene Hemocytes In Vitro Infection GLS Analysis

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Effect of an electron gas on a neutron-rich nuclear pasta

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Nuclear Science and Techniques 2015年第2期26卷 75-82页

作者： jorge Alberto lopez Enrique Ramirez-Homs Department of Physics University of Texas at El Paso

We studied the role that electron gas has on the formation of nuclear structures at subsaturation densities and low temperatures(T<1 Me V).Using a classical molecular dynamics model we studied isospin symmetric and asymmetric matter at subsaturation densities and low temperatures varying the Coulomb interaction *** effect of such variation was quantified on the fragment size multiplicity,the inter-particle distance,the isospin content of the clusters,the nucleon mobility and cluster persistence,and on the nuclear structure *** found that the presence of an electron gas distributes matter more evenly,disrupts the formation of larger objects,reduces the isospin content,and modifies the nucleon average displacement,but does not affect the inter-nucleon distance in *** nuclear structures are also found to change shapes by different degrees depending on their isospin content,temperature and density.

关键词：电子气体分子动力学模型面食中子相互作用强度核结构同位旋平均位移

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Surface Modification by Nitrogen Plasma Immersion Ion Implantation on Austenitic AISI 304 Stainless Steel

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Journal of Iron and Steel Research International 2016年第4期23卷 380-384页

作者： Miguel CASTRO-COLIN William DURRER jorge A. lopez Enrique RAMIREZ-HOMS Max Plank Institute for Intelligente Systems Stuttgart 70569Baden-wurttembergGermany Physics Department University of TexasEl Paso TX79968-0515TexasUSA

Surfaces of AISI 304 austenitic stainless steel plates nitrided by plasma immersion ion implantation （PⅢ） technology were studied by means of Auger electron spectroscopy （AES） and X-ray photoelectron spectroscopy （XPS） to determine the effect of the nitriding process on the surface and subjacent layers. Elemental compositions obtained by AES and XPS at varying depths indicate that the saturation of N is relatively constant as a function of depth, indicating the reliability of PⅢ technology for substirface saturation. It is concluded that the concentrations of both Cr and O increase with depth, the subjacent oxide is driven by the Ar^＋ sputtering process used to access the lower layers, and then N is bound to Cr.

关键词： PⅢ technology AISI 304 XPS AES surface modification

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Properties of nuclear pastas

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Frontiers of physics 2021年第2期16卷 119-173页

作者： jorge A.lopez Claudio O.Dorso Guillermo Frank Department of Physics University of Texax at El PasoEl PasoTexas 79968USA Departamento de Fisica FCENUniversidad de Buenos AiresNunezArgentina Unidad de Investigacion y Desarrollo de las Ingenierias Universidad Tecnologica NacionalFacultad Regional Buenos AiresBuenos AiresArentina

In this review we study the nuclear pastas as they are expected to be formed in neutron star *** start with a study of the pastas formed in nuclear matter(composed of protons and neutrons),we follow with the role of the electron gas on the formation of pastas,and we then investigate the pastas in neutron star matter(nuclear matter embedded in an electron gas).Nuclear matter(NM)at intermediate temperatures(1 MeV≤T≤***),at saturation and and sub-saturation densities,and with proton content ranging from 30%to 50%was found to have liquid,gaseous and liquid-gas mixed *** isospin-dependent phase diagram was obtained along with the critical points,and the symmetry energy was calculated and compared to experimental data and other *** low temperatures(T≤1 MeV)NM produces crystal-like structures around satura-tion densities,and pasta-like structures at sub-saturation *** of the pasta structures were studied with cluster-recognition algorithms,caloric curve,the radial distribution function,the Lindemann coefficient,Kolmogorov statistics,Minkowski functionals;the symmetry energy of the pasta showed a connection with its *** star matter(NSM)is nuclear matter embedded in an electron *** electron gas is included in the calculation by the inclusion of an screened Coulomb *** connect the NM pastas with those in neutron star matter(NSM),the role the strength and screening length of the Coulomb interaction have on the formation of the pastas in NM was *** was found to exist even without the presence of the electron gas,but the effect of the Coulomb interaction is to form more defined pasta structures,among other ***,it was determined that there is a minimal screening length for the developed structures to be independent of the cell *** star matter was found to have similar phases as NM,phase transitions,symmetry energy,structure function and thermal *** in NM,pasta f

关键词： nuclear pasta neutron star matter nuclear symmetry energy molecular dynamics nuclear phase transitions

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Mechanical and natural ventilation systems in a greenhouse designed using computational fluid dynamics

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International Journal of Agricultural and Biological Engineering 2014年第1期7卷 1-16页

作者： jorge Flores-Velazquez Juan I.Montero Esteban J.Baeza Juan C.lopez Mexican Institute of Water Technology.Paseo Cuauhnáhuac 8532 Col.ProgresoC.P.62550JiutepecMor.Mexico Institut de Recerca i Tecnologia Agroalimentàries 08348Cabrils(Barcelona)Spain IFAPA Centro La Mojonera(Junta de Andalucía) Camino San Nicolás 104745 La MojoneraAlmeríaSpain Fundación Cajamar.Estación Experimental“Las Palmerillas”Autovía del Mediterráneo Km 416 704710El EjidoAlmeríaSpain

The aim of this study was to analyse air exchange and temperature distribution in a greenhouse with combined mechanical and natural ventilation and to design more efficient mechanical ventilation *** this purpose,a computational fluid dynamics(CFD)model of the greenhouse was *** configurations were considered:Configuration 1(mechanical ventilation and closed roof ventilators),Configurations 2 and 3(mechanical ventilation and roof ventilators open 30%and 100%,respectively).After validation,the CFD model was used to improve the design of the greenhouse mechanical ventilation system in each of the three configurations *** greenhouse lengths,28 m,50 m,75 m and 100 m,were used in the *** to fan ventilation only,roof ventilation improved the climate of fan-ventilated greenhouses in terms of the air exchange rate(22%)and climate uniformity because the internal air was mixed better than with mechanical ventilation *** the greenhouse length increased,more advantages were achieved with natural ventilation compared to mechanical *** most configurations,there was a strong linear correlation between temperature gradient and greenhouse *** greenhouse whose regression line had the steepest slope was the one with closed roof *** the fan capacity produced a general reduction in temperature,but the effect was less intense for the greenhouses with open roof *** to box inlet ventilators,an enlarged continuous inlet in the wall opposite the fans increased overall system performance because it eliminated backflow recirculation zones,which are prone to produce high temperatures.

关键词： greenhouse cooling fan ventilation roof ventilators combined ventilation

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Helmholtz Decomposition of Vector Fields Using an Optimal Preconditioned Conjugate Gradient Algorithm

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Journal of Applied Mathematics and Physics 2023年第5期11卷 1337-1348页

作者： jorge lopez UJAT Tabasco México

In this article, we study numerically a Helmholtz decomposition methodology, based on a formulation of the mathematical model as a saddle-point problem. We use a preconditioned conjugate gradient algorithm, applied to an associated operator equation of elliptic type, to solve the problem. To solve the elliptic partial differential equations, we use a second order mixed finite element approximation for discretization. We show, using 2-D synthetic vector fields, that this approach, yields very accurate solutions at a low computational cost compared to traditional methods with the same order of approximation.

关键词： Helmholtz Decomposition Self-Adjoint Operator Optimal Preconditioning Finite Element

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Lesser kestrels of the same colony do not overwinter together

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Current Zoology 2024年第4期70卷 505-512页

作者： jorge Garcia-Macia Munir Chaouni Sara Morollon Javier Bustamante Lina lopez-Ricaurte Juan Martinez-Dalmau Beatriz Rodriguez-Moreno Vicente Urios Grupo de Investigacion Zoologia de Vertebrados Universidad de AlicanteApdo.99E-03080 AlicanteSpain Department of Wetland Ecology Estacion Biologica de Donana(CSIC)C/Americo Vespucio 2641092SevillaSpain GREFA C/MontedelPilarS/NE-28220MajadahondaMadridSpain

Migratory connectivity describes the linkage between breeding and nonbreeding sites,having major ecological implications in birds:1 season influence the success of an individual or a population in the following *** studies on migratory connectivity have used large-scale approaches,often considering regional populations,but fine-scale studies are also necessary to understand colony *** lesser kestrel Falco naumanni,an insectivorous migratory raptor which form colonies during the breeding period,was considered to have strong connectivity based on regional ***,no small-scale studies on migratory connectivity have been ***,we GPS(Global Positioning System)-tracked 40 adult lesser kestrels from 15 different Spanish breeding colonies,estimating the overlap index between home ranges and the distance between their *** was found that lesser kestrels from the same breeding colony placed their nonbreeding areas at 347±281 km(mean±standard deviation)away from each other(range=23-990),and their home ranges over-lapped by 38.4±23.6%.No differences between intra-colony and inter-colony metrics were found,which suggests that lesser kestrels from the same breeding cluster do not overwinter together,but they spread out and mixed independently of the colony belonging throughout the nonbreeding range of the ***,this study highlights the importance of performing connectivity studies using fine-scale approaches.

关键词： Falco naumanni GPS telemetry migratory connectivity nonbreeding raptor spatial ecology wintering.

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Eucalyptus grandis plantations:effects of management on soil carbon,nutrient contents and yields

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Journal of Forestry Research 2020年第2期31卷 601-611页

作者： Diego Martin Sandoval lopez Marcelo Fabian Arturi Juan Francisco Goya Carolina Alejandra Perez jorge Luis Frangi Laboratorio de Investigacion de Sistemas Ecologicos y Ambientales(LISEA) Universidad Nacional de La PlataDiagonal 113 N°4691900 La PlataBuenos AiresArgentina

The expansion of fast-growing tree plantations is a worldwide process,with consequences on soil fertility and soil carbon *** results were found on the effects of afforestation with Eucalyptus on soil carbon and other nutrient *** discrepancies are usually caused by differences in climate,land use history,soil texture as well as by management related factors such as plantation age,number of rotations,method of establishment(plantation or coppice),harvest residue management and soil *** studied the effect of plantation age,number of rotations,and method of establishment on soils and plant nutrient concentrations in Eucalyptus grandis plantations in NE Argentina on different textured *** also determined if yields changed with nutrient variations in soils,and compared soils under plantations to soils under grasslands they ***-one *** stands of different ages,number of rotations and method of establishment were evaluated as well as eight grassland *** of carbon,nitrogen,phosphorus,potassium,calcium and magnesium were determined for soils and *** carbon and nitrogen decreased over the number of rotations and were more pronounced in soils with 50-60%sand than soils with>75%*** stands showed higher soil carbon and nitrogen levels than plantations,suggesting a negative effect of site preparation before planting on soil nutrient conservation,especially in fine-textured *** nutrient concentrations did not follow the trends observed for soil nutrients nor did they reflect nutrient *** was no evidence of decreased yields over successive *** carbon and nitrogen contents decrease when grasslands are replaced by *** plantations,and therefore a yield limitation may occur in a medium to long-term frame,especially in stands re-established for short-rotation *** residue management and site preparation must be specifically designed for improving so

关键词： Soil texture Grasslands Foliar nutrient concentrations Successive harvest Coppice Eucalyptus grandis

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The variability of juvenile dispersal in an opportunistic raptor

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Current Zoology 2023年第3期69卷 244-254页

作者： jorge Garcia-Macia Gabriel lopez-Poveda Javier De La Puente Ana Bermejo-Bermejo Manuel Galan Ernesto Alvarez Sara Morollon Vicente Urios Vertebrates Zoology Research Group University of AlicanteApdo.99E-03080 AlicanteSpain SEO/BirdLife Bird Monitoring UnitC/Melquiades Biencinto34E-28053MadridSpain GREFA C/MontedelPilarS/NE-28220 MajadahondaMadridSpain

The juvenile dispersal of raptors is a crucial stage that stretches from parental independence to the establishment of the first breeding *** 2012 and 2020,44 juvenile red kites Milvus milvus from the Spanish breeding population were tagged using GPS telemetry to study their *** left the parental breeding area at the end of their first summer and performed wandering movements throughout the Iberian Peninsula,returning to the parental breeding area the following year,repeating the same pattern until they settled in their first breeding *** analyzed the mean distance from the nest,the maximum reached distances,and the traveled distances(daily and hourly)during the first 2 years of dispersal and compared *** the high individual variability,variables describing the dispersal movements of juveniles showed a decreasing trend during the second dispersal year:80% of individuals reached a shorter maximum distance in the second year,70% decreased their mean distance to the nest,65% decreased their hourly traveled distances,and 50% decreased their daily traveled *** the other hand,the red kites usually combined wandering movements with the establishment of temporary settlement areas(TSA).The average duration of settlement in the TSAs was 75±40 days(up to 182 days)and was located at 182±168 km from the *** those areas,juveniles used 781.0±1895.0 km^(2)(KDE 95%).Some of the TSAs were used by several individuals,which suggests that these areas might be good targets for conservation in future management plans.

关键词： GPS telemetry landfills movement ecology natal dispersal red kite spatial ecology

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Increased virulence of the oral microbiome in oral squamous cell carcinoma revealed by metatranscriptome analyses

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International Journal of Oral Science 2018年第4期10卷 263-272页

作者： Susan Yost Philip Stashenko Yoonhee Choi Maria Kukuruzinska Caroline A.Genco Andrew Salama Ellen O.Weinberg Carolyn D.Kramer jorge Frias-lopez Forsyth Institute 245 First StreetCambridgeMA 02142USA Boston University Henry M.Goldman School of Dental Medicine 100 East Newton StreetBostonMA 02118USA Department of Integrative Physiology and Pathobiology Tufts University School of Medicine136 Harrison AvenueBostonMA 02111USA Department of Oral Biology College of DentistryUniversity of Florida1395 Center DriveGainesvilleFL 32610-0424USA

Oral squamous cell carcinoma(OSCC) is the most prevalent and most commonly studied oral cancer. However, there is a void regarding the role that the oral microbiome may play in OSCC. Although the relationship between microbial community composition and OSCC has been thoroughly investigated, microbial profiles of the human microbiome in cancer are understudied. Here we performed a small pilot study of community-wide metatranscriptome analysis to profile mRNA expression in the entire oral microbiome in OSCC to reveal molecular functions associated with this disease. Fusobacteria showed a statistically significantly higher number of transcripts at tumour sites and tumour-adjacent sites of cancer patients compared to the healthy controls analysed. Regardless of the community composition, specific metabolic signatures were consistently found in disease. Activities such as iron ion transport, tryptophanase activity, peptidase activities and superoxide dismutase were over-represented in tumour and tumour-adjacent samples when compared to the healthy controls. The expression of putative virulence factors in the oral communities associated with OSCC showed that activities related to capsule biosynthesis, flagellum synthesis and assembly, chemotaxis, iron transport, haemolysins and adhesins were upregulated at tumour sites. Moreover, activities associated with protection against reactive nitrogen intermediates, chemotaxis, flagellar and capsule biosynthesis were also upregulated in non-tumour sites of cancer patients. Although they are preliminary, our results further suggest that Fusobacteria may be the leading phylogenetic group responsible for the increase in expression of virulence factors in the oral microbiome of OSCC patients.

关键词： profiles analyses

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