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Heat shock protein family D member 1 in boar spermatozoa is strongly related to the litter size of inseminated sows

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Journal of Animal Science and Biotechnology 2022年第4期13卷 1045-1055页

作者： Won-Ki Pang Ji-Hyun Son Do-Yeal Ryu Md Saidur Rahman Yoo-Jin park Myung-geol Pang Department of Animal Science&Technology and BET Research Institute Chung-Ang UniversityAnseongGyeonggi-do 17546Republic of Korea

Background:Sperm quality evaluation is the logical first step in increasing field *** contain cytoplasmic organelles and biomolecules known as sperm-intrinsic factors,which play key roles in sperm maturation,sperm-oocyte fusion,and embryo *** particular,sperm membrane proteins[e.g.,arginine vasopressin receptor 2,beta-actin,prohibitin,and heat shock protein family D member 1(HSPD1)]and RNA could be used as functional indicators of male *** sought to clarify the effects of differential mRNA expression of selected genes on several fertilisation parameters,including sperm motility,motion kinematics,capacitation,and litter size,in a porcine ***:Our results demonstrated that HSPD1 expression was significantly correlated with male fertility,as measured by the litter size of inseminated *** expression of HSPD1 mRNA was linked to sperm motility and other motion kinematic ***,HSPD1 had a 66.7%overall accuracy in detecting male fertility,and the high-litter size group which was selected with the HSPD1 marker had a 1.34 greater litter size than the lowlitter size ***:Our findings indicate that HSPD1 might be a helpful biomarker for superior boar selection for artificial insemination,which could boost field fertility.

关键词： Fertilisation HSPD1 Male fertility Sperm motility Sperm RNA

Proteomic identification of cryostress in epididymal spermatozoa

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Journal of Animal Science and Biotechnology 2017年第2期8卷 478-489页

作者： Sung Jae Yoon Md Saidur Rahman Woo Sung Kwon Do Yeal Ryu Yoo Jin park Myung geol Pang Department of Animal Science&Technology Chung-Ang UniversityAnseongGyeonggi-Do 456-756Republic of Korea

Background： Cryopreservation of epididymal spermatozoa is important in cases in which it is not possible to collect semen using normal methods, as the sudden death of an animal or a catastrophic injury. However, the freezing and thawing processes cause stress to spermatozoa, including cold shock, osmotic damage, and ice crystal formation,thereby reducing sperm quality. We assessed the motility（%）, motion kinematics, capacitation status, and viability of spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence ***, we identified proteins associated with cryostress using a proteomic approach and performed western blotting to validate two-dimensional electrophoresis（2-DE） results using two commercial ***： Cryopreservation reduced viability（%）, motility（%）, straight-line velocity（VSL）, average path velocity（VAP）, amplitude of lateral head displacement（ALH）, and capacitated spermatozoa, whereas straightness（STR）and the acrosome reaction increased after cryopreservation（P 3 fold, P ＆lt; 0.05） before and after cryopreservation. The proteins differentially expressed following cryopreservation are putatively related to several signaling pathways, including the ephrinR-actin pathway, the ROS metabolism pathway, actin cytoskeleton assembly, actin cytoskeleton regulation,and the guanylate cyclase ***： The results of the current study provide information on epididymal sperm proteome dynamics and possible protein markers of cryo-stress during cryopreservation. This information will further the basic understanding of cryopreservation and aid future studies aiming to identify the mechanism of cryostress responses.

关键词： Cryopreservation Cryostress Protein Spermatozoa

Rapid detection of influenza A(H1N1)virus by conductive polymer-based nanoparticle via optical response to virus-specific binding

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Nano Research 2022年第3期15卷 2254-2262页

作者： Geunseon park Hyun-Ouk Kim jong-Woo Lim Chaewon park Minjoo Yeom Daesub Song Seungjoo Haam Department of Chemical&Biomolecular Engineering Yonsei UniversitySeoul 03722Republic of Korea Division of Chemical Engineering and Bioengineering College of Art Culture and EngineeringKangwon National UniversityChuncheon-siGangwon-do 24341Republic of Korea Biohealth-machinery Convergence Engineering Kangwon National UniversityChuncheonGangwon-do 24341Republic of Korea College of Pharmacy Korea UniversitySejong 30019Republic of Korea

A recurrent pandemic with unpredictable viral nature has implied the need for a rapid diagnostic technology to facilitate timely and appropriate countermeasures against viral *** this study,conductive polymer-based nanoparticles have been developed as a tool for rapid diagnosis of influenza A(H1N1)*** distinctive property of a conductive polymer that transduces stimulus to respond,enabled immediate optical signal processing for the specific recognition of H1N1 *** poly(aniline-co-pyrrole)-encapsulated polymeric vesicles,functionalized with peptides,were fabricated for the specific recognition of H1N1 *** low solubility of conductive polymers was successfully improved by employing vesicles consisting of amphiphilic copolymers,facilitating the viral titer-dependent production of the optical *** optical response of the detection system to the binding event with H1N1,a mechanical stimulation,was extensively analyzed and provided concordant information on viral titers of H1N1 virus in 15 *** specificity toward the H1N1 virus was experimentally demonstrated via a negative optical response against the control group,***,the designed system that transduces the optical response to the target-specific binding can be a rapid tool for the diagnosis of H1N1.

关键词： influenza A(H1N1)virus conductive polymer optical property rapid detection

Improvement of Shape Recognition Performance of Sendzimir Mill Control Systems Using Echo State Neural Networks

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Journal of Iron and Steel Research International 2014年第3期21卷 321-327页

作者： Jung-hyun park Seong-ik HAN jong-shik KIM School of Mechanical Engineering Pusan National University School of Electrical Engineering Pusan National University

High rigidity twenty-high Sendzimir mills （ZRMs） are widely used for rolling stainless steels, silicon sheets, etc. A ZRM uses a small diameter work roll to produce massive rolling forces. Since a work roll with a small diameter can be bent easily, strips often have complex shapes with mixed quarter and deep edge waves in the shape of plates. In order to solve this problem, fuzzy neural network controls are generally used for shape： recognition in ZRM control systems. Among various neural network types, the multi-layer perceptron （MLP） is typically used in current ZRMs. However, an MLP causes the loss of a large amount of shape recognition data. To improve the shape recognition per- formance of ZRM control systems, echo state networks （ESNs） are proposed to be used. Through simulation re- sults, it is found that shape recognition performance could be improved using the proposed ESN method.

关键词： Sendzimir mill neural network multi-layer perceptron echo state network shape recognition

Design,fabrication,and structural safety validation of 3D-printable biporous bone augments

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Bio-Design and Manufacturing 2023年第1期6卷 26-37页

作者： Yeokyung Kang Dasol Lim Doo-Hoon Sun jong-Chul park Jungsung Kim Central Research&Development Center Corentec Company Ltd.33-2Banpo-daero 20-gilSeocho-guSeoulRepublic of Korea Cellbiocontrol Laboratory Department of Medical EngineeringYonsei University College of Medicine50-1 Yonsei-roSinchon-dongSeodaemun-guSeoulRepublic of Korea Department of Orthopaedic Surgery Daejeon Sun Hospital10-7 Mok-dongJung-guDaejeonRepublic of Korea

The use of commercial products such as a cup and liner for total hip arthroplasty for patients with severe bone defects has a high probability of *** these patients the cup alone cannot cover the bone defect,and thus,an additional augment or cage is *** this study,we designed three-dimensional(3D)printable bone augments as an alternative to surgeries using reinforcement ***-five sharp-edged bone augments of various sizes were 3D printed.A biporous structure was designed to reduce the weight of the augment and to facilitate bone *** types of frames were used to prevent damage to the augment’s porous structure and maintain its stability during ***,two types of holes were provided for easy augment fixation at various *** tests were performed on a combination of worst-case sizes derived using finite element *** test results confirmed the structural stability of the specimens at a load of 5340 *** the porosity of the specimens was measured to be 63.70%,it cannot be said that the porous nature was uniformly distributed because porosity tests were performed locally and *** summary,3D-printable biporous bone augments capable of bonding from various angles and bidirectionally through angulation and bottom-plane screw holes are *** mechanical results with bone augments indicate good structural safety in ***,further research is necessary to study the clinical applications of the proposed bone augment.

关键词： Bone augment 3D printing Biporous structure Total hip arthroplasty Metal implant

Microstructural evolution of pre-twinned Mg alloy with annealing temperature and underlying boundary migration mechanism

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Journal of Magnesium and Alloys 2023年第8期11卷 2953-2966页

作者： Ye Jin Kim jong Un Lee Gyo Myeong Lee Sung Hyuk park School of Materials Science and Engineering Kyungpook National UniversityDaegu 41566Republic of Korea

This study investigates the variations in the microstructural characteristics of a pre-twinned Mg alloy with the temperature of the subsequent annealing *** this end,a rolled AZ31 alloy is compressed to 3%plastic strain along the rolling direction(RD)to activate{10-12}twinning and is subsequently annealed at 200,250,300,350,and 400℃.Numerous{10-12}twins are formed throughout the compressed material,leading to the formation of a RD-oriented *** an annealing temperature of 200℃,no microstructural variations occur during *** the annealing temperature increases from 250 to 400℃,the residual strain energy and remaining twin boundaries of the annealed material decrease owing to the promoted static recovery and the increased area fraction of twin-free grown ***,an increase in the annealing temperature results in a gradual microstructural transition from a fully twinned grain structure to a completely twin-free grain *** microstructural evolution during annealing is predominantly governed by the movement of high-angle grain boundaries via a strain-induced boundary migration mechanism,and a few twin boundaries migrate above 350℃because of their lower boundary *** boundary migration behavior and resultant microstructural evolution are discussed in detail based on the variations in boundary mobility and driving force for boundary migration with annealing temperature.

关键词： Rolled Mg alloy {10-12}twin Grain growth Boundary migration Annealing

Interfacial Behaviors of Basalt Fiber‑Reinforced Polymeric Composites: A Short Review

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Advanced Fiber Materials 2022年第6期4卷 1414-1433页

作者： Seong‑Hwang Kim jong‑Hoon Lee jong‑Woo Kim Seul‑Yi Lee Soo‑Jin park Department of Chemistry Inha University100 InharoIncheon 22212South Korea

Basalt fibers(BFs)have emerged as a promising thermal insulation material for various applications,such as fireproof clothes/walls and protective equipment in military and civil *** have many desirable characteristics,such as low thermal conductivity,excellent flame resistance,exceptional mechanical strength,facile manipulability,environmental friendliness,and ***,the low intrinsic interfacial properties of BFs due to their chemical inertness and macro-scaled sizes have been a challenge for high performance of BFs-reinforced polymeric composites(BFRPs).Since the mechanical properties of BFRPs significantly depend on the interfacial interaction between the fibers and the matrix,it is critical to understand how incorporating BFs influences the properties of the *** this end,the aim of this review is to report on recent research progress with emphasis on the interfacial behavior in *** relationships between the fiber–matrix interfacial adhesion and the mechanical properties of the BFRPs are briefly described with systematic and up-to-date surface modification techniques summarized into two categories:surface modifications(“wet”and“dry”)and multi-scaled ***,several strategies for increasing the interfacial adhesion of BFs within the polymeric matrix to provide new ideas and insight for future research on the BFRPs are discussed.

关键词： Basalt fibers Polymeric matrix Surface modification Interface

Establishment of a male fertility prediction model with sperm RNA markers in pigs as a translational animal model

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Journal of Animal Science and Biotechnology 2022年第6期13卷 1743-1753页

作者： Won-Ki Pang Shehreen Amjad Do-Yeal Ryu Elikanah Olusayo Adegoke Md Saidur Rahman Yoo-Jin park Myung-geol Pang Department of Animal Science&Technology and BET Research Institute Chung-Ang UniversityAnseongGyeonggi-do 17546Republic of Korea

Background:Male infertility is an important issue that causes low production in the animal *** solve the male fertility crisis in the animal industry,the prediction of sperm quality is the most important *** RNA is the potential marker for male fertility *** hypothesized that the expression of functional genes related to fertilization will be the best target for male fertility prediction *** investigate optimum male fertility prediction marker,we compared target genes expression level and a wide range of field data acquired from artificial insemination of boar ***:Among the genes related to acrosomal vesicle exocytosis and sperm–oocyte fusion,equatorin(EQTN),zona pellucida sperm-binding protein 4(ZP4),and sperm acrosome membrane-associated protein 3 exhibited high accuracy(70%,90%,and 70%,respectively)as markers to evaluate male *** of EQTN-ZP4,ZP4-protein unc-13 homolog B,and ZP4-regulating synaptic membrane exocytosis protein 1(RIMS1)showed the highest prediction value,and all these markers are involved in the acrosome ***:The EQTN-ZP4 model was efficient in clustering the high-fertility group and may be useful for selection of animal that has superior fertility in the livestock *** to the EQTN-ZP4 model,the ZP4-RIMS1 model was more efficient in clustering the low-fertility group and may be useful in the diagnosis of male infertility in humans and other *** appointed translational animal model and established biomarker combination can be widely used in various scientific fields such as biomedical science.

关键词： Male fertility Multiple markers Pig model Prediction model Sperm RNA

Non-isotropic weighted L^p estimates for the -equation on piecewise smooth strictly pseudoconvex domains

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Science China Mathematics 2008年第12期51卷 2161-2171页

作者： Heungju AHN Hong Rae CHO jong-Do park Nims 3F TowerKoreana 628 Daeduk-Boulevard Yuseong-gu Department of Mathematics Pusan National University Department of Mathematics Seoul National University Department of Mathematics Pohang University of Science and Technology

In this paper we obtain non-isotropic weighted Lp estimates with the boundary distance weight function for the-equation on piecewise smooth strictly pseudoconvex domains under a hypoth- esis of complex transversalit... 详细信息

关键词： non-isotropic norm Cauchy-Riemann equation piecewise strictly pseudoconvex domain